Present study was designed to isolate and characterize the persistent organic pollutants (POPs) metabolizing bacteria from tannery effluents and petrol contaminated soil samples collected from gasoline stations of Bahawalpur, Pakistan. For the isolation of individual bacteria capable of metabolizing different POPs methods of selective enrichment culture and serial dilution were employed. Followed by isolation, growth behavior, corresponding POPs removal efficiencies, biochemical characteristics and antibiotics resistance profiles were documented. For molecular analysis, 16S rRNA gene was amplified and sequenced. Retrieved sequences were analyzed through BLAST analysis and to reflect relationship phylogenetic trees were constructed using software MEGA version 7. To detect the possible metabolic pathways responsible for POPs degradation, the isolates with high removal efficiencies were selected. Extracellular and intracellular metabolites of the selected bacteria were extracted and subjected to GC-MS and LC-MS analysis. Effect of selected carbon source on proteins production of bacteria was elucidated through LC-MS analysis of the intracellular proteins extracted from various bacterial isolates. The 16S rRNA metagenome sequencing was performed in order to confirm the POPs metabolizing potential of isolated bacteria among the bacterial community of tannery effluent and petrol contaminated soil. Shotgun metagenome sequencing helped to determine the possible genes and pathways associated with POPs degradation. Total forty-one bacteria were isolated during present study of which twenty-eight were toluene metabolizing, four were naphthalene metabolizing and nine were petrol metabolizing bacteria. The top BLAST similarity analysis revealed similarity of isolates with Brevibacillus agri strain NBRC 15538 (n = 27), Bacillus paralicheniformis strain KJ-16 (n = 4), Burkholderia lata strain 383 (n = 6), Burkholderia pyrrocinia strain LMG 14191 (n = 1) and Brevibacillus formosus strain NBRC 15716 (n = 3). All isolates were found sensitive to teicoplanin and linezolid and resistant to oxacillin and chloramphenicol. However, two bacteria, IUBT9 and IUBT26, exhibited sensitivity to oxacillin. Present study bacteria were examined to have considerable toluene removal potential (28-93%), naphthalene removal potential (70-83%) and petrol removal potential (41-69.5%). Biochemical characterization revealed presence of many valuable enzymes like arginine dehydrolase, naphthylamidase, glucosaminidase and phosphatase and ability of isolates to ferment variety of sugars like esculin, mannitol, sorbitol and inulin. GC-MS and LC-MS based analysis of bacterial metabolites confirmed degradation potential of the isolated bacteria to metabolize toluene, naphthalene and petrol through not only the earlier reported metabolic pathways but also some novel metabolic pathways which have not been reported so far. LC-MS analysis of bacterial proteins revealed the presence of enzymes involved in the degradation of toluene, synthesis of secondary metabolites, central dogma of gene expression, chemotaxis, spore development, transport of ions and central carbon metabolism further confirming the utilization of the provided carbon sources (toluene, naphthalene, petrol) by present study bacteria.
اردو کے اہم مدونین (بابائے اردومولوی عبدالحق) حالات زندگی: مولوی عبدا لحق برِ صغیر پاک ہند کے عظیم اردو مفکر، محقق، ماہر لسانیات، معلم، بانی انجمن ترقی اردو اور اردو کالج کے بانی تھے، ا?پ کو بابائے اردو کے لقب سے یاد کیا جاتا ہے۔آپ کی تاریخ پیدائش کے حوالے سے کافی اختلاف پایا جاتا ہے ،لیکن بقول ممتاز حسین مولوی عبدالحق 20 اپریل،1870ء کوبرطانوی ہندوستان کے ضلع میرٹھ کے ہاپوڑ کے قریب سراوہ نامی ایک گاؤں میں پیدا ہوئے۔مولوی صاحب کے والد شیخ علی حسین نے عہد مغلیہ میں اسلام قبول کیا۔ان کے سپرد محکمہ مال کی اہم خدمات رہیں۔مغلیہ دور میں انہیں جو مراعات حاصل تھیں وہ انگریز وں کے دور میں بھی برقرار رہیں۔ تعلیم و تربیت: مولوی عبدالحق نے ابتدائی تعلیم گھر پرحاصل کی۔ثانوی تعلیم میرٹھ سے حاصل کی۔ 1894ء میں علی گڑھ سے بی اے کیا۔علی گڑھ میں سر سید احمد خان کی شخصیت سے بہت متاثر ہوئے۔1895ء میں حیدر آباد کے ایک سکول میں ملازمت اختیار کی۔اورنگ آباد میں صدر محتتم تعلیمات کے عہدے پر فائز رہے۔عثمانیہ کالج اورنگ آباد میں 1920ء تک بحیثیت پرنسپل فرائض سر انجام دیتے رہے۔ انجمن ترقی اردو: جنوری 1902ء میں آل انڈیا محمڈن ایجوکیشن کانفرنس علی گڑھ کے تحت ایک علمی شعبہ قائم کیا گیا جس کانام انجمن ترقی اردو تھا۔ مولانا شبلی نعمانی اس کے سیکرٹری رہے تھے۔ عزیز مرزا کے بعد 1912ء میں مولوی عبدالحق سیکرٹری منتخب ہوئے جنھوں نے بہت جلد انجمن ترقی اردو کو ایک فعال ترین علمی ادارہ بنا دیا۔ مولوی عبدالحق اورنگ آباد (دکن ) میں ملازم تھے ،وہ انجمن کو اپنے ساتھ لے گئے اور اس طرح حیدر آباد دکن اس کا مرکز بن گیا۔ فروغ اردو کے لیے انجمن کی خدمات: انجمن کے زیر اہتمام ایک لاکھ سے زائد جدیدعلمی ، فنی اور سائنسی اصطلاحات کا اردو...
Human beings are the masterpieces of the Creator of the universe and He enjoined them to preserve human life. One form of the preservation of life is the utmost care for the rights of even an embryo. The study of the rights of the embryo laid down in the primary scriptures of Islam suggests that the embryo is accorded respect like living people. The rights of children start well before their birth. At present, cases ofabortion are reported daily. Muslim jurists have dwelled on ordinances about abortion for the benefit of people. At present, medical science has revealed all the stages of childbirth from conception to delivery. The effects of abortion, both positive and negative, are not hard to grasp in the light of modern scientific research Not to talk of living people, Islam guarantees the life of the early developmental stage of a baby within the uterus of the mother. This extraordinary care for a child during its embryonic period demonstrates that Islam attaches high priority to human life even before its start on the face of the Earth. Should one abort without a legitimate cause, the legal experts from the main schools of Islamic jurisprudence hold that the perpetrator will pay a fine and make compensation. If, on the other hand, a fetus is removed from the womb to end a pregnancy as it may harm a woman physically, it is allowable; only a healthy mother may give birth to healthy babies. In a like manner, when a couple feels unable to train and educate somany kids, they may have an operation to end a pregnancy by removing an embryo. However, while resorting to abortion, parents must not lose sight of the do’s and don’ts of abortion jurists have laid down and not just fall victim to the catchy slogan of “family planning”.
Teachers‟ self-efficacy in inclusive settings works as a strong agent to promote inclusive education. Purposely it is important to investigate the level of self-efficacy of regular school teachers who are expecting to work in inclusive classroom. Present study was a practical attempt to investigate the level of self-efficacy of regular classroom teachers for teaching in inclusive classrooms. Survey research design has been used in this study to measure self-efficacy of regular school teachers who were teaching at primary, elementary and secondary grade levels in all regular schools were taken as population of the study. For sampling, purposive sampling was done to select twelve districts out of thirty six (36) districts from Punjab Province on the basis of density of their population. As sample total 500 teachers who were teaching at primary, elementary and secondary grade levels were selected through simple random sampling procedure. Data was collected through a self-designed scale named “Teacher self-efficacy in inclusive settings (TSEIS)”. Through this scale regular classroom teachers‟ self- efficacy was measured on five level Likert scale raging; (1) I am very much confident, (2) I am confident, (3) No opinion (4) I am less confident, (5) I am not confident. Indicators for measuring self-efficacy of regular teachers were respectively as follow; (1) teachers‟ efficacy for need assessment for differentiated instructions (2) teachers‟ efficacy for adapting assessment tools (3) teacher‟s efficacy for managing classroom disruptive behaviors of diverse learners in inclusive settings (4) teachers‟ motivation for teaching diverse learners in inclusive settings (5) teacher‟s efficacy for collaborating with parents in inclusive settings (6) teacher‟s efficacy for teaching in collaboration with other professionals through team teaching techniques. Finally, completely filled tools were collected back from 446 teachers. The return response rate was 90% from the respondents. The reliability of the tool was 0.823. Through findings of present study the researcher found negative relationship between gender and self-efficacy components used in study tool. The principal analysis of 06 components yielded the value (0.424) of only adaptation in assessment which was less than 0.5 that showed the reinvestigation of remaining five components. Regression analysis of students‟ need assessment for differentiated instruction as reported by general education teachers found them untrained to assess the needs of diverse students. Through this study the researcher concluded that implementation of full inclusion would not be possible unless regular classroom teachers would ready to work. At the end it is recommended that school administration and the stakeholders may plan better school improvement initiatives while they get the reflection of each teacher‟s personal professional feedback in accordance to their professional development demands for future.