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Home > Isolation, Purification and Characterization of Zn++-Dependent Acid Phosphatase from Chicken’S Heart and its Comparison With the Enzyme of Chicken’S Liver

Isolation, Purification and Characterization of Zn++-Dependent Acid Phosphatase from Chicken’S Heart and its Comparison With the Enzyme of Chicken’S Liver

Thesis Info

Access Option

External Link

Author

Saeed, Asma

Program

PhD

Institute

Gomal University

City

Dera Ismail Khan

Province

KPK

Country

Pakistan

Thesis Completing Year

2009

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/2845/1/315S.pdf

Added

2021-02-17 19:49:13

Modified

2023-01-06 19:20:37

ARI ID

1676726557814

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زلفی بھٹو

زلفی بھٹو

                                                                                                                                محمد احمد ترازی

زلفی بھٹو تاریخ پاکستان کا لازوال کردار ہے ۔

یہ اپریل 1945ء کی بات ہے جب تحریک پاکستان کے قائد اعظم محمد علی جناح کی ولولہ انگیز قیادت میں اپنے بام عروج پر تھی اور مسلمانانِ ہند کے ’’لے کے رہیں گے پاکستان بٹ کے رہے گا ہندوستان‘‘کے نعروںسے پورا برصغیر گونج رہا تھا ۔بچے ،بوڑھے اور جوان سب کا ایک ہی مطالبہ تھا ایک آزاد و خود مختار سر زمین کا حصول جس میں وہ اپنی زندگی اپنی معاشرتی روایات اور مذہبی اقدار کے مطابق بسر کر سکیں ۔گویا حصول پاکستان مسلمانانِ برصغیر کا خواب ہی نہیں ان کی جدو جہد کی حقیقی منزل بھی تھا اس زمانے میں ایک طالب علم نے اپنے محبوب لیڈر قائد اعظم محمد علی جناح کو ایک خط  لکھا ۔جس میں اس نے لکھا ۔’’ڈئیر سر ! صوبہ سرحد میں سیاسی صورتحال پیدا ہوئی ہے اس نے مجھے اتنا جذباتی اور برانگیختہ کر دیا ہے کہ میں اپنے قائد کو اس کے متعلق کچھ لکھنے کی جرأت کر رہا ہوں ۔ ایسا معلوم ہوتا ہے کہ آج کے مسلمانوںکو یہ محسو س کر لینا چاہیے کہ ہندو بنیے ہمارے ساتھ کبھی مخلص و متحد نہیں ہو سکتے وہ ہمارے قرآن پاک اور ہمارے پیغمبر کے شدید ترین دشمن ہیں ۔یہ بھی اچھی طرح جان لینا چاہیے کہ آپ ہی ہمارے قائد اوررہنما ہیں ۔جناب آپ نے ہمیں ایک پلیٹ فارم اور ایک جھنڈے تلے اکٹھا کیا ہے اور ہر مسلمان کا یہی نعرہ ہے کہ ’’پاکستان کی طرف بڑھو ،ہماری قسمت پاکستان ہے ‘‘ہماری منزل و مقصد پاکستان ہے ۔ہمیں آپ کی ذات میں ایک قابل رہنما مل گیا ہے ۔اب ہمیں کوئی بھی منزل مقصود کی طرف جانے سے نہیں روک سکتا ۔میں...

اقتصادی بحران اور ہنگامی صورتحال پر قابو پانے کیلئے سیدنا عمر کے اقدامات کا تحقیقی جائزہ

Hazrat Abdul Rehman (may Allah be pleased with him) belonged to Arab tribe of Quraish and was a close relative of Mohammad (peace be upon him). At the time of conquest of Makkah He (may Allah be pleased with him) entered the circle of Islam. He (may Allah be pleased with him) is counted among the companions of Muhammad (may Allah be pleased with him) who came to sub-continent specially Balochistan in order to preach for Islam and Jihad during the Khilafat of orthodox caliphs. He (may Allah be pleased with him) came to Balochistan twice for Jihad and conquests first during the Khilafat of Hazrat Usman (may Allah be pleased with him) and second time in the early era of Hazrat Muawia (may Allah be pleased with him). He (may Allah be pleased with him) played a vital role in the wars of Balochistan. He (may Allah be pleased with him) established Zehri his abode and capital after conquering Kalat, Khuazdar (Sajistan), Kachi, Gandhava, and Chaghi, and from here he expanded the series of his conquests till Kabul and Qandar. Besides this, he included many areas of sub-continent in the Islamic empire of conquered areas. His (may Allah be pleased with him) life is consists of great chapters of sincerity in deeds. Wisdom and valor, determination fearlessness, strife, hospitality, simplicity and patience. He (may Allah be pleased with him) is counted among the great generals of Islam had the honour to have carried the message of Holy faith in every corner of Balochistan in tough and unfavorable conditions and planted the flag of Islam in Balochistan forever.  

Synthesis of Biocompatible Self-Assembling Amphiphiles for Vesicular Drug Delivery Applications.

Oral route is preferred for drug administration due to convenience in dose administration, lack of pain, effectiveness and increased patient compliance. Drug’s decreased aqueous solubility, gastric instability, enzymatic degradation, decreased biological membrane permeability, P-gp based drug efflux, first pass metabolism, mucus membrane barrier and early clearance from the body are various factors leading to poor oral bioavailability and less therapeutic efficacy of orally administered drugs. Conventional drug dosage forms have not been effective in addressing these issues. Recently, nanotechnology has got much scientific interests in various fields. The biomedical applications of nano-carriers have been the subject of recent interests due to their evident role in addressing the above mentioned drug related issues. Amphiphilic molecules are capable of self-assembling into vesicles upon their contact with aqueous media. They are able to dissolve both hydrophilic and lipophilic drugs either in their interior aqueous compartment or exterior lipid bilayer respectively. Self-assembling amphiphilic molecules like phospholipids, nonionic surfactants, block copolymers and peptides are widely used for designing vesicular nanocarrier drug delivery systems. They are physically stable, economic, efficient and release the loaded drug in a sustain manner, thus leading to enhance oral bioavailability with better clinical outcomes. The current study was designed for the synthesis of four different biocompatible amphiphiles for nano-vesicular drug delivery applications. They were synthesized in neucleophilic substitution reactions by reacting hydrophilic moieties (Creatinine and Pyridoxine) with lipophilic acyl chlorides and aliphatic alkyl bromide respectively. The Vitamin E based amphiphile was synthesized in multi step reaction to introduce polar hydrophilic moiety in lipophilic alpha tocopherol (Vitamin E). These synthetic amphiphiles were characterized through 1H NMR and xii Mass spectrometric analytical techniques. All the amphiphiles were subjected to biocompatibility studies and their nano-vesicular drug delivery applications were investigated using model poorly aqueous soluble drugs i.e. Clarithromycin, Azithromycin and Amphotericin B. All the amphiphiles were less cytotoxic, hemo-compatible and non-toxic to animals. They were capable of forming nano-vesicles of almost spherical shape with homogenous size distribution. The drug loaded vesicles exhibited increased surface negativity and loaded increased amounts of the selected model drugs. They released the loaded drugs in a controlled pattern at various pHs when tested for in-vitro release. Amphiphiles based drug loaded vesicles demonstrated higher storage stability and retained maximum drugs upon incubation with simulated gastric fluid. Invivo bioavailability of the selected drugs was increased in a controlled manner in rabbits upon their oral administration in the form of amphiphiles based nano-vesicles. Results of the current study authenticates biocompatibility of these synthesized amphiphiles and their effectiveness for oral drug delivery of poor aqueous soluble drugs. The results are interesting and provide a scientific base for their use in vesicular drug delivery systems. The results will open new research areas for formulation scientists to investigate their membrane permeability mechanisms with ultimate increased therapeutic efficacy of loaded drugs.