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Home > Kras Oncogenic Mutation Pattern in Pakistani Patients Presenting With Colorectal Adenocarcinoma in a Northern Tertiary Referal Centre

Kras Oncogenic Mutation Pattern in Pakistani Patients Presenting With Colorectal Adenocarcinoma in a Northern Tertiary Referal Centre

Thesis Info

Access Option

External Link

Author

Qayyum, Azhar.

Program

PhD

Institute

University of Health Sciences

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Pathology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9363/1/Azhar%20Qayyum_HIstopatholoby_2016_UHS_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726572360

Similar


Veterinary Pathology Pathology Abstract Colorectal cancer is a global disease with marked morbidity and mortality. Involvement of genetic and environmental factors produces variability in its prevalence. KRAS (Kirsten rat sarcoma viral oncogene homologue) is one of the important genes responsible for sporadic CRC. The constitutive GTPase activity of the gene is lost when it gets mutated and so the deregulated cells undergo unrestricted proliferation leading to oncogenesis. From therapeutic point of view, patients with KRAS mutations do not benefit from epidermal growth factor receptor inhibition drugs as those who possess normal KRAS. The analysis of KRAS mutations is therefore important from both diagnostic as well as therapeutic purposes. Diagnostic methodology and the valuable information of this study will be quite helpful in future. The objectives of this study were to document the KRAS oncogenic mutation pattern mostly in Northern Pakistani residents presenting in AFIP, Rawalpindi after their intestinal resection due to CRC. The other objective was to relate this mutation pattern with histopathological categorical variables like age, gender, presenting clinical complaint, tumor site, size, histological differentiation, modified Duke Stage of tumor, nodal metastasis and metastasis in other organs. Hundred and eighty one cases of CRC from persons residing in northern area of Pakistan were collected and observed for the above mentioned parameters. DNA was extracted from the tumors and normal tissues of all the study subjects. PCR was done for gene amplification and the status of KRAS mutations in Pakistani CRC patients was analyzed by gene sequencing. The results showed more prevalence of CRC in the males of northern areas of Pakistan. Both males and females with higher average age had high tendency to acquire CRC. Rectum was found to be the most common location of CRC, followed by sigmoid. Exophytic well differentiated adenocarcinomas were the most common form of CRC. Gene sequencing did not show even a single KRAS mutation in the tested samples. It can be inferred from this study that either there is no KRAS mutation in northern area patients suffering from CRC or the mutation frequency of this particular gene is relatively very low as compared to the results of studies reported in the literature. A wider scale study of these patients may prove the cause of CRC to be due to mutation of some other genes or may be due to some non-genetic factors.
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غزل

 جیون کی

 

 اس درد کتھا کا

 

میں اک عنواں ہوں

 

میرے اندر صدیوں کا

 

سنتاپ چھپا ہے

 

میرے  باطن میں ہیں کتنے

 

 گہرے گہرے  درد  سمندر

 

جن کی تشنہ سی کچھ لہریں

 

میرے بنجر خوابوں کی

 

تعبیریں ڈھونڈ رہی ہیں

 

خواب سفر کی منزل

 

کتنی کٹھن ہوئی ہے

 

میں اس منزل کا راہی ہوں

 

اپنی ذات کے صحراؤں میں

 

سر گرداں ہوں

 

یعنی آج کا انساں ہوں میں

 

حقوق الإنسان المدنية من خلال وثيقة المدينة: دراسة مقارنة بالمواثيق الدولية

حقوق الإنسان المدنية من خلال وثيقة المدينة: دراسة مقارنة بالمواثيق الدولية This research aims to give the reader a comprehensive view of civil human rights through a comparative study of the Charter of Medina and international conventions. The Charter of Medina designed the foundation of a multi-religious Islamic state in Medina, as it was signed to end the rancorous intertribal aggression among the opposing tribes of Banu ’Aws and Banu Khazraj in Medina and to uphold harmony and co-operation among all Medinan groups. Its major accomplishment was fetching confrontational clans together to form a community and inaugurating long term peace among them. It put an end to the predominant disorder and sheltered the life, self-determination, property and religious freedom for all people. The paper highlights the relevance and importance of civil human rights through the Charter of Medina as well as international conventions in the up-to-date worldwide civilization. The present research examines the historical document of Charter of Medina and elucidates it through examples from Quran and Sunnah as well as compares its core values with international conventions. In this regard the views of the past and contemporary scholarship are also discussed to analyze the challenges and issues of current time. In recent times the efforts and implications of civil human rights have unfolded in many different ways so it is very important for Muslims to know and to relate the Sharī’ah ruling regarding it. The research concludes that as compare to the international convections the system of justice in the Islamic Sharī’ah ensures all rights and with liabilities.

Impact of Changing Women Status on Pakhtun Society.

Prior to the independence of Pakistan, during the Muslim and British rule in india subcontinent was mostly based on customs and environment produced by the integration of various cultural and religious groups. Status accorded to women was not subordination and they faced economic inequality and male domination. With the passage of time and the advancement of western education through increased impact on intellectual, social, political and economic issues of Europe and the Muslim world in the 19 th century influenced the traditional society and the role of women. Change in the status of women from that of subordination to gender equality was becoming visible in the middle of 20 th century, and at the same time, cultural and traditional practices continued to have a strong hold and influence on the status of women in the region, which brought forth a new situation of restlessness in traditional structure on one hand and empowerment of women on the other. Continuous efforts had been made by the government and private sector to recognize the ability, competence and input of women in the development of society at national and regional levels. The researcher having the social science background was interested to understand the trends and practices toward women and their status in Pakhtun society. This study had been carried out to explore various factors influencing the traditional society in general and women with their ascribed and prescribed roles. The study focused on the following objectives. 1. To find out the factors influencing the life of Pakhtun woman in her social setup. 2. To find out factors bringing change of status of woman in society. 3. To find out the impact of change of woman status on family structure. 4. To explore problems faced by men and women due to change in the status of women. The study focussed on five districts of Khyber Pakhtunkhwa i.e Peshawar, Mardan, Charsadda, Nowshera and Swabi with equal gender representation of geographical area. The data was collected from 500 respondents including 250 females and 250 males with equal representation of rural and urban areas. The study was based on the following hypothesis. 1. Educated Pakhtun women are empowered and have improved status in the society and the findings are that the research hypothesis was not accepted.(reference page:189) vi 2. Educated Pakhtun women are empowered in making independent decisions and the findings are that the hypothesis was not accepted. (reference page:193) 3. Working woman gets respect and love in the family and the findings are that the hypothesis was not accepted. (reference page:195) 4. Improved economic status of woman has psychologically empowered her in Pakhtun society and the findings are that the research hypothesis was not accepted. (reference page:198) Major findings of the study revealed that education, science, technology and employment of women were the basic tools and instrument of both economic growth and social change which lead to enhance women status and their participation in decision making process. The research findings showed that majority 83.8 percent respondents (both males and females), that females are involved in the family decisions, while 16.2 percent respondents declined any such involvement. With reference to participation of women in political activities, it was found that men still enjoy decisive power in allowing women to contest in elections casting vote or supporting any political party independently. Further it was found that 51.2 percent male encouraged and 48.8 percent were reluctant to allow women participation in politics. Regarding the share of women’s ownership in property very few Pakhtun women own property, despite of the injunctions of sharia law in respect of female inheritance. The study findings show that 45.8 percent of the respondents (including both male and female) reported that females were not given share in property due to cultural constraints pertaining to patrilineal kinship structure, remission of haq mehr, dowry, and bride price. Arranged marriages still persist, gradually accommodating educated children’s consent regarding selection of marriage partners. Regarding spouse selection of marriage 71.2 percent females reported that their marriages were arranged by parents or in their families, 26.8 percent female respondents reported that the marriages of females in their family were arranged by the parents with their consent. Education and employment are enabling factors to give women a status of controlling family activities systematically. Violence against women still persists. 44.4 percent female respondents reported violence in their families while 55.6 percent female respondents denied violence in their families.