Plant growth promoting rhizobacteria (PGPR) are the soil and plant associated bacteria that promote growth and yield of plant by various mechanisms. PGPR are important in agriculture as it has replaced the use of chemical fertilizers, pesticides and other supplements. Rhizosphere microorganisms produce the substances that promote growth in large quantities that affect plant’s morphology indirectly. In the proposed study, root nodulating bacteria were isolated from chickpea and pea. Their nodulation activity, biofilm formation, root colonization were observed and characterized for solubilization of phosphate, production of indole acetic acid and fixation of nitrogen. Potential root nodulating bacteria were identified using 16S rRNA, ITS and nodC gene sequence analysis. AHLs were detected using thin layer chromatography. Potential root nodulating bacteria were evaluated to enhance yield and growth of chickpea and pea. All the strains were analyzed for growth promotion abilities in growth chamber experiment. Soil analysis was done before pot experiment. Strains were evaluated for their improvement in yield and growth of chickpea and peas. Rhizobium bangladeshense was isolated from pea nodules for the first time. Overall Mesorhizobium ciceri strain CM1 and CM2 showed the best results on chickpea as compared to control. It was confirmed for the first time that Rhizobium bangladeshense nodulates pea. Rhizobium bagladeshense PM3 and Rhizobium leguminosarum strains PM1 showed the best results on peas. Co-inoculation with Rhizobium bangladeshense PM3 and Rhizobium leguminosarum PM2 strain showed the best result on peas as compared to single inoculation.