Group A rotavirus (RVA) is the leading cause of diarrhea in children <5 years in many countries. The objectives of this pre-vaccination era study were to explore the prevalence and the genetic diversity of RVA strains in <5 years children, hospitalized due to gastroenteritis during 2014 – 2016. Two tertiary care hospitals Benazir Bhutto Hospital (BBH) located in Rawalpindi and Kharadar General Hospital (KGH) in Karachi were the study sites. A total of 1227 children were tested through ELISA for the presence of RVA and 28.5% (n=350) were found positive. From the 956 children enrolled in BBH (n=502 in 2014 and n=454 in 2015), 29.1% (n=279) were RVA positive. Among the 271 children enrolled in KGH during 2016, 26% (n=71) were found to be infected with RVA. A majority (78%; n=272) of RVA gastroenteritis cases were found in children <1 year of age and the virus was detected throughout the study period. Genotyping of ELISA positive samples through RT-PCR showed G12P[6] (21%) as the most dominant genotype followed by G3P[8] (16%), G2P[4] (12%), G1P[8] (9%), G9P[6] (8%) and G3P[6] (6%). A high proportion (10%) of mixed infection was also observed. Phylogenetic analysis clustered Pakistani G1 strains into two lineages, G1 lineage 1 & 2 along with strains from Russia, Australia, Thailand, India, Bhutan, Belgium Turkey, and the USA. The G2 strains clustered into G2 lineage IV, sub lineage IVa-3 along with strains reported worldwide. Pakistani G2 strains showed the D96N and S242N substitutions which are characteristic of sub-lineage IVa-3 and have been linked to the reemergence of these strains in many countries. Pakistan G3 viruses clustered into G3 lineage 3, sub-lineage 3d and were closely related to strains from China, Russia, Japan, and the USA. High amino acid similarities existed among indigenous G3 and the new variant G3 viruses that were first reported from Japan in 2003-2004. We hereby report the first finding of these variant G3 viruses from our country. The G9 strains grouped into two lineages G9 lineage 3 & 4 with the majority of strains belonging to lineage 3. Pakistani G12 strains belonged to the G12 lineage 3 together with G12 from different countries. The P[4], P[6] and P[8] Pakistani strains were linked to VP4 genotypes found globally. Comparative analysis of wild-type rotaviruses with RotarixTM and RotaTeqTM revealed several amino acid differences in the VP7 and VP8* antigenic epitopes. Notably, Pakistani G3 isolates contained a K238N amino acid change that generates an extra N linked glycosylation site which could have an effect on the antigenicity of these strains. This is the first report on the predominance of G12P[6] and the emergence of G3P[8] from Pakistan. Our findings provide important information pertinent to the genetic diversity of RVA strains circulating in the two cities of Pakistan (Rawalpindi and Karachi) and emphasize the need for large-scale epidemiological studies across the country.
قدیم مصری تہذیب میں اکثر و بیشتر بادشاہ اپنی بہن سے شادی کرتا حتیٰ کہ بیٹی سے بھی شادی رچائی جاتی تھی ۔اس کے لیے تاویل یہ پیش کی جاتی کہ شاہی خون خالص رہے۔ فرعونی دور کی تحریروں کو جب ڈی کوڈ کیا گیا تو معلوم ہو ا کہ مصری شاعری میں لفظ بھائی بہن محبوب اور محبوبہ کے معنوںمیں بھی استعمال ہوتا تھا ۔بادشاہوں کے حرموں میں بہنوں کے علاوہ سینکڑوں کنیزیں رکھنے کا شوق اپنی جگہ مگر متوسط آمدنی والے مصر کے عام لوگ یک زوجگی پر قانع رہتے تھے ۔خانگی زندگی بدیہی طور پر بڑی حد تک بہتر تھی۔عورت کو طلاق دینا آسان نہ تھا ۔عقد میں آنے والی عورت کو جائیداد میں اچھا خاصا حصہ ملتا۔ایک مغربی مفکر کا قول ہے کہ کسی بھی قدیم یا جدید تہذیب نے عورت کو وہ بلند قانونی رتبہ نہیں دیا جتنا وادی ِ نیل کے باشندوں نے دیا۔ اپنی تند خو (سقراطی) بیویوں کو گھر میں بند رکھنے کے عادی یونانی سیاح یہ آزادی دیکھ کر ششدر رہ جاتے ۔ فرعونی دور کے ادب میں عورت کی حیثیت اور عظمت کے گُن گائے جاتے تھے۔ مصری عورت سے محبت ایک قومی فریضہ سمجھا جاتا تھا۔ مصری مرد کو صرف مصری عورت سے ہی قلبی اور جنسی وابستگی کی ترغیب دی جاتی۔ ایک مصری بزرگ اپنے سننے والوں کو سمجھاتے ہیں کہ’’ باہر سے آنے والی ایسی عورتوں سے ہوشیار رہو ۔یہ گہرے پانیوں کے بھنور کی مانند ہوتی ہیں‘‘۔اسی طرح ایک مصری اپنے بیٹے کو نصیحت کرتے ہوئے لکھتا ہے کہ’’اگر تم نے اپنا گھر کامیابی کے ساتھ سجا سنوار لیا ہے اور خوب صورت ترین بیوی تمھاری آغوش میں ہے تو اس کا پیٹ بھرو اور کمر پر کپڑا ڈالو۔اس کی خوشی کا سامان مہیا کرو کیوں...
Japan and the land of present Pakistan had cultural linkages since ages. Sindh has remained important part of this land. The Indus civilization had trade links with other countries including Sumerian and Babylonian civilizations. This trend continued afterwards, and trade relations were established between people of Sindh and Japan in the nineteenth century. This interaction led both the countries to have cultural linkages. Presently many Sindhis are residing in Japan who have adopted Japanese culture besides maintaining their own cultural traditions and customs. Sindhi poetry adopted Japanese Haiku and ‘Safarnamas’ were written in Sindhi language grasping the living and traditions, etc. Of Japan. This research paper also discusses that both countries’ people have similar style of sitting together and exchange of opinions and happenings of the day which is traditionally called ‘Katchehry’ in Sindh.
The concept of organizational learning is area of attention for academicians and practitioners because of its
potential to sustain the organization performance while the SMEs are an emerging business structures in
Pakistan. The organizational learning contributes significantly in the value of performance of corporate and as
well to small business structures. The empirical purpose of this study was to investigate impact of
organizational learning on perceived SMEs performance and further with moderation of leadership role.
The theoretical consideration was empirically tested through research design of cross-sectional, non-contrived
and IT base industry of SMEs. The survey was conducted for collection of inductive data and 129 respondents
were valid and considered for analysis. The statistical tests were applied for empirical investigation and results
reveal that; there is significant contribution of organizational learning in perceived SMEs performance and each
dimension of organizational learning is also significant predictor of perceived SMEs performance while shared
vision has higher rank among system thinking, personal mastery, mental model and team learning.
The further results depict that leadership role is not significant moderator in relationship of organizational
learning and perceived SMEs performance however team leadership may moderate this relationship which is
also recommended for future research in IT base SMEs which configuring the organizational learning and
performance.