Search or add a thesis

Advanced Search (Beta)
Home > Molecular and Functional Characterization of Bioactive Peptides from the Venom of Bracon Hebetor Say. Hymenoptera: Braconidae

Molecular and Functional Characterization of Bioactive Peptides from the Venom of Bracon Hebetor Say. Hymenoptera: Braconidae

Thesis Info

Access Option

External Link

Author

Manzoor, Atif

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/3086/1/Atif_Manzoor_Agri_Entomology_HSR_UAF_2016_13.02.2017.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726674349

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


Venom is a key-factor in the regulation of host physiology by parasitic Hymenoptera and a potentially rich source of novel bioactive substances for biotechnological applications. The limited study of venom of ectoparasitic wasp Bracon hebetor (Say.) (Hymenoptera, Braconidae) has not described the full complement and composition of these biomolecules or the biotechnological potential that they could represent. To provide a more complete insight, the transcripts expressed in the venom glands of the wasp B. hebetor were described using a high-throughput approach with Illumina technology. The transcriptome of the B. hebetor venom glands was analysed by high throughput sequencing using Illumina technology followed by de novo assemblies of the 18,143,814 sequence reads yielded 22,425 contigs of which 10,578 have significant BLASTx homologies in the available databases. The majority of sequence homology was with the ectoparasitoid, Diachasma alloeum, a wasp from the same taxonomic family. Homology was also observed with other wasps, bees, and ants. Gene ontology was used to group sequences by molecular functions in which catalytic activity with 42.2% was the most highly represented, cellular components comprised 33.8% of homologies and biological and metabolic processes represented 30% of the relationships. In this study, we highlight the most abundant sequences, and those that are likely to be functional components of the venom for parasitization. Specifically, we focused on genes encoding proteins that are reported in the literature to be involved in host developmental arrest, disrupting the host immune system, host paralysis, and transcripts that support these functions. The full length ORFs of Calreticulin, the Venom Acid Phosphatase Acph-1 like protein and Arginine Kinase proteins were isolated and their tissue specific expression was studied by RT-PCR. The data showed that these putative venom genes were not expressed in tissues other than venom glands and whole females. Cloning of these genes was also done successfully and Calreticulin was further processed for expression analysis. Functional analysis of the venom of the wasp was performed by artificial microinjections of both crude and treated venom (heat and proteinase) of the wasp containing 0.3 μl and 0.5 μl in non-parasitized and synchronized host insects. Last instars of the host greater wax moth Galleria mellonella (Lepidoptera, Pyralidae) were selected for venom injection bioassays and mortality data of the host insects was recorded after 1, 12, and 24 hours of venom injections while host receiving saline injections were acted as control. Our findings demonstrate that maximum mortality of the host insects (98%) was obtained by microinjections containing volume (0.5 μl) of crude venom. On the other hand heat and proteinase treated venom did not show any significant mortality of the host insect. This is the first report of large-scale analysis of genes transcribed by the venomous gland of the wasp B. hebetor. In addition, results also demonstrate that de novo transcriptome assembly allows useful venom gene expression analysis in a species lacking a genome sequence database, which ultimately provides useful information for devising control tools for insect pests of Pyralidae and other applications.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

مرشیہ مرشد پاک

مرثیہ مرشد پاک
(مرثیہ جو مرشد پاک کے وصال کے بعد دربار پر حاضری کے وقت پڑھا گیا )

تان ساڑھی وچ ماڑی سوں گیوں دلدار وے
عاشق خالی ویکھ حجرہ رون ڈھائیں مار وے

سخن تیرے یاد کرکے رون زارو نذار وے
نکل آ، لا محفلاں، توں آ سُہا دربار وے

کون دیسی آن تسلیاں شہزادیاں نوں یار وے
کون دھرسی ہتھ سر تے ‘ کون کرسی پیار وے

عاشقاں دربار ملّا رون سانگاں مار وے
کد ملسی یار سوہنا بنھ کے دستار وے

حضورمحمد فیض علی شاہ ہَے نام سوہنا دل دیندا ٹھار وے
عاشقاں نے تانگ رکھی آ کرادے دیدار وے

سنج کچہری کر چلے عاشقاں سالار وے
باہجھ دلبر یار جانی جیونا بیکار وے

ویکھ جگہ مجلس والی نین مینہ برسائوندے
کتھ گئے دلدار جانی جیہڑے رونقاں لائوندے

باغ تیرا ذکر والا باہجھ تیرے سکیا
قمر نوری حسن والا ہیٹھ بدلی لکیا

نین کجلے دار تیرے عاشقاں من بھائوندے
حسن والے مان کردے عاشقاں ترسائوندے

اے محبوبا ویکھ تینوں نین کدی نہ رجدے
نوری مکھڑا حسن بھریا زلفاں کنڈل پھبدے

قادری سائیںؔ مرشد دے در رو رو ہاڑے پائوندا
باہجھ تیرے اے محبوبا چین نہ ہے آئوندا

منو دھرم شاستر اور ہندو نظام میراث

It is said that “Mony makes the mare go ” and it is so because AH’ah Subhdn-au-Taa’la Has willed it so. Wealth is thus, a great blessing and a means of great trial for man at the same time. It is utility and worth-no matter how a man may amass wealth- is restricted to this world alone. A man enters into the world hereafter-empty handedly. All his treasured troves of gold and silver are bequeathed to his loved ones after his demise. Had there been no proper mechanism or a modus-oerandi for the division of this left-over money/inheritance then violence and bloody feuds would have sparked amongst the heirs. Hence we find that almost all religions offer guidelines for the division of ( bequeathed mony or) wealth and valuables left after the death of a person. So is the case of Hindu religion. The purpose of this article is to focus, assess and analyze the Hindu system of inheritance-as to how to disperse the left-over wealth amongst the closest relatives of the decease

Dynamical Control Systems: Design, Modelling, Simulation in Distributed and Local Environment

The work presented in this thesis addresses the dynamical control systems regarding manufacturing, industrial processing and transportation, in local and distributed environment adapting simplified design techniques. This enhances the control strategies giving multi-agents based autonomous capabilities. A new design model of fuzzy logic discrete event (DEV) control system under time constrain is proposed and implemented for industrial applications. Three systems: grinding and mixing fuzzy logic time control system, liquids mixing fuzzy logic time control system, and multi- dimensional supervisory control industrial processing system using fuzzy time control are designed. In this regard, a simplified design approach is adapted to reduce the complexity of memory based fuzzy systems and to enhance the controllability and stability of the systems. Design of: fuzzifier, inference engine, rule base, deffuzifiers, and DEV control system, is discussed. Time control fuzzy rules are formulated, applied and tested using MATLAB simulation for the systems. The simulation results of each proposed application are found in agreement with the design based calculated results. For vehicles automation, multi-agents based autonomous railway vehicles control model is designed. In this regard, a new speed scheduling, management and control model is established to meet the requirements of modern autonomous train systems. This research work proposes to develop a novel control system to enhance the efficiency of the vehicles under constraints of various conditions: hard conditions; junction track condition, track clearance, and crossing gates condition, flexible conditions; environment monitoring, track condition, and tilting condition. Various development techniques to establish the multi-agents based autonomous railway vehicles control system are discussed and proposed for implementation using high tech microelectronics technology. The design and simulation work is carried out at the laboratories of GC University Lahore Pakistan, and The School of Electronics and Engineering-SEE, Edinburgh University U.K during research fellowship to work with system level integration group SLIg using MATLAB-simulink, and Xilinx 10.1 suit for ISE and DSP tools.