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Home > Molecular Characterization of Local Human Extraintestinal Escherichia Coli Isolates

Molecular Characterization of Local Human Extraintestinal Escherichia Coli Isolates

Thesis Info

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Author

Bashir, Saira

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2010

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1801

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726686579

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Extraintestinal pathogenic E.coli are major pathogens responsible for causing urinary tract infections. These are associated with multiple drug resistance (MDR) and high virulence. A triplex PCR was employed to segregate each isolate into different phylogenetic groups. Among fifty nine uropathogenic E.coli isolates majority belonged to phylogenetic group B2 (50%) where as 19% each belonged to groups A and B1, and 12% to group D. Phylogenetic group D isolates were found to be less frequent but harbored multiple drug resistance and virulence related genes to a greater extent. Drug resistance was evaluated by disc diffusion method and relevant drug resistance gene detection. It was found that most effective drugs were gentamicin and amikacin among aminoglycosides; aztreonam, ceftriaxone, cefoperazone and cefixime among β lactams, and ciprofloxacin among quinolones. Streptomycin, trimethoprim-sulfamethoxazole, tetracycline and nalidixic acid were relatively ineffective. Chloramphenicol was found to be very effective for urinary tract infections. Highly prevalent drug resistance gene was mutated gyrA (83%) followed by tem (46%), bltTEM (41%), tetB (34%), catP (31%), and tem β lactamase (15%); blaSHV and tetA were not detected. Among virulence related genes most prevalent was hlyA (37%) followed by sfaDE (27%), papC (24%), cnf1 (20%), eae A (19%) and afaBC3 (14%). Some of isolates were highly hemolytic to human erythrocytes and some were highly cytotoxic to Vero cells. Highly hemolytic isolates mainly belonged to group D, where as highly verotoxic isolates belonged to group B2 followed by group D. It was also noted that in cnf1 negative isolates cytotoxicity was related to papC, sfaDE, afaBC3, eae A and hlyA genes.
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کامل مرشد دی پہچان

کامل مُرشد دی پہچان

پھڑ دامن پیر منا لَے توں
اِنج سوکھا ای رب نوں پا لَے توں
رکھ پہلی پوڑی پیر میاں
انج کردا جا توں سیر میاں
تیری ہر دم ہووے خیر میاں
من اپنے نوں سمجھا لَے توں
پھڑ دامن پیر منا لَے توں
پھڑ مرشد کر انکار نہیں
بنِ مرشد بیڑا پار نہیں
لینی ہور کسے وی سار نہیں
اُس در تے ڈیرے لا لَے توں
پھڑ دامن پیر منا لَے توں
اوہ بستی یار مدینہ ہے
اوتھے ملدا نور خزینہ ہے
اوتھے ہوندا ٹھنڈا سینہ ہے
کر ہمت درشن پا لَے توں
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سوہنا مرشد ماڑی والا اے
جس کیتا نور اجالا اے
سدا وسن جھوکاں شالا اے
اُس در توں فیض کما لَے توں
پھڑ دامن پیر منا لَے توں

ایہو طرز طریقہ سارا جے
ایہو سب توں راز نیارا جے
اُس رب دا نام پیارا جے
اُس نام دا ورد پکا لَے توں
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ڈِٹھا خواجہ عبد رحمن میاں
ہِن دُھماں وچ جہان میاں
جتھے جھکدے شاہ سلطان میاں
اُس در توں فقر کما لَے توں
پھڑ دامن پیر منا لَے توں

اوہ مٹھا ہے خلق طبیعت دا
پابند اے بڑا شریعت دا
اوہ اچھا ہے ذہنیت دا
اوہدے در تے کلّی پا لَے توں
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اوہدا میتھوں سن توں حالا نی
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وچ ماڑی جھوکاں لائیاں نیں
اوتھے ذکری جلیاں پائیاں نیں
گیاں ہر جاء ہو...

Agile Practices and Intention to Stay: Mediation Effects Through Job Characteristics

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Trust Based Security Framework for Secure Routing in Mobile Ad Hoc Networks

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