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Molecular Detection of Salmonella Typhi Strains & Their Drug Resistance Pattern

Thesis Info

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Author

Haque, Asma

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2005

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1864

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726697726

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Typhoid fever has become a major problem due to the emergence of MDR strains of Salmonella enterica serovar Typhi, the causative agent, at an alarming rate during recent years. The situation is worsened due to lack of quick, sensitive and reliable diagnostic tools for determining the drug resistance pattern. Conventional methods are time consuming and lack sensitivity. It was envisaged that a multiplex PCR diagnosing typhoid and detecting resistance against standard typhoid drugs, ampicillin, chloramphenicol, trimethoprim-sulfamethoxazole and ciprofloxacin would be very useful. After determining drug resistance patterns by standard disc diffusion method on a pool of 23 MDR S. Typhi isolates, a PCR amplification technique was used for various drug resistance related genes found universally in S. Typhi. These were tem, catP, and sul2 genes. None of these isolates was resistant to ciprofloxacin, so a fragment of gyrA gene (related to ciprofloxacin resistance) was amplified from an MDR E.coli isolate, cloned, and transformed into an MDR S. Typhi isolate that was naturally resistant to other drugs. A regular multiplex PCR was subsequently developed by using this cloned bacterium which was followed by the development of a nested multiplex PCR for increasing specificity and sensitivity. This diagnostic multiplex PCR has been successfully optimized to be directly applicable to clinical samples.
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ملکِ عرب

ملک عرب:
محل وقوع: براعظم ایشیاء کے جنوب مغرب میں واقع ملک عرب کے تین اطراف سمندر اور چوتھی طرف خشکی ہے ۔ مغرب میں بحیرہ قلزم ہے جو مکہ معظمہ سے مغربی جانب تقریباََ ستتر (۷۷) کلو میٹر کے فاصلہ پر ہے ۔اس کے علاوہ مغرب میں آبنائے سویز اور بحیرہ روم ہے ۔ مشرق میں خلیج فارس اور عمان ‘ جنوب میں بحرہند اور اس کے شمال میں شام و عراق اور شمال سے جنوب تک ایک سلسلہ کوہ پھیلا ہو ا ہے‘ جسے جبل السراۃ کہتے ہیں ‘ شمال میں یہ شام اور فلسطین کے پہاڑوں سے جا ملتا ہے ۔ اس سلسلہ کو جگہ جگہ سے وادیاں قطع کرتی ہیں ۔ سلسلہ جبال السراۃ کو توریت میں سلسلہ کو ہ فاران کا نام دیا گیا ہے ۔ ( سیرت النبی اعلان نبوت سے پہلے ۔ٖص۲۰)جغرافیہ کے ماہرین نے اس ملک کو آٹھ حصوں میں تقسیم کیا ہے ۔ جن میں سے ایک حجاز ہے ۔ چونکہ ’’تہامہ ‘‘ اور ’’ نجد ‘‘ کے درمیان حاجز ہے جو تہا مہ اور نجد کو الگ کرتا ہے اس لیے ملک کے اس حصہ کو حجاز کہتے ہیں ۔ حجاز کا زیادہ حصہ بنجر اور بے آب وگیاہ ہے جسے قرآن مجید نے ’’ وادی غیر ذی زرع‘‘ کہا ہے ۔اس میں صدیوں سے موجود (عرب کا قدیم شہر مکہ )یا بکہ ایک تجارتی مرکز تھا ۔ یمن ،شام اور حبشہ کے مابین تجارتی قافلوں کی گزر گاہ پر واقع تھا ۔ یہا ں کے باشندے بھی خوب تجارت کرتے تھے ۔ یمن اور حبشہ کی مصنوعات اور پیداوار خرید لاتے اور انہیں مصر اور شام کے بازاروں میں جا کر فروخت کرتے ۔وہ بصری ٰاور دمشق کے میلوں سے بھی سامان خرید لاتے اوراس کے بدلے میں اپنا سامان فروخت کرتے تھے ۔موسم گرما...

Historical, Cultural and Geographical Ties Between Pakistan and Iran

In order to get a vivid picture of Pakistan-Iran relations, the paper delves into the past with Iranian history and Iranian sphere of influence in South Asia. Both South Asia and Iran have ancient historical, cultural and religious ties since the times of the Cyrus the Great to the present. The Persian language and literature particularly the Persian classical poetry have left a great impression on sub-continent’s cultural and educational canvass. Pakistan-Iran border which was drawn by the British in the 1880s, remains still intact. Though the border is a wasteland with little agriculture but on the sea-front both the countries have access to the Strait of Hormuz which is of great strategic importance. Moreover, ethnic Baloch live on both sides of the border and crossborder and trade and livestock is the mainstay of cross-border economy. During the Cold War period, both Pakistan and Iran were subservient to the US policies against the Soviet Union; consequently, both received massive arms and equipment. This paper also indicates that during Mohammed Reza Shah’s time, the relations between Pakistan and Iran were quite friendly but they underwent a change after the Iranian revolution and during General Zia’s time. The main issues have been spelled out up-till 1979 and the minor frictions between the two countries could be managed through diplomacy. External pressures are also influencing the contemporary relations between the two countries and have put Pakistan in a dilemma. Historical and secondary sources have been used for the conduct of the study. Historical research has an important role to play in the Social Sciences. It helps us to understand the present by highlighting the past events.

Investigating of Nano Composite Materials for out Door Insulation Applications

This research deals with the preparation of silicone rubber (SiR), epoxy and EPDM based composite materials for outdoor high voltage insulationapplications. These composites were fabricated by incorporating nanosilica 2.5~5 % by weight, while the microsilica loading were varied from 10~20 % by weight. Uniform and homogenous dispersion of fillers were achieved through process parameters optimization. The tensile strength of fabricated polymer compositesincreases with the incorporation of filler contents. Even in case of hybrid composites, it increases up to 400 %. The prepared composite samples were found thermally more stable than their virgin counterparts. An approximate 25% increase in dielectric strength was also obtained. The novelty of the research work is promise of nano and hybrid composite towards their aging performance under multi-stress conditions. To comprehend the aging phenomena (weathering characteristics) under multi-stress conditions, the entirepolymer composites were aged for a maximum of 5,000 hrs in a multi-stress aging chamber developed as per IEEE standard 1109. Different stresses like ultraviolet, thermal, salt-fog, acid rain and voltage were applied for specifically designed weather cycles. After multi-stress aging according to the data of hydrophobicity loss silicone rubber composites were placed in HC-3 while epoxy and EPDM composites were grouped in HC-4 and HC-5 respectively. The results of leakage current during the multi-stress aging for silicone rubber, epoxy and EPDM composites were found 0.8μA, 1μA and 1.2μA correspondingly. The hybrid polymer composites due to synergic effects responded better towards aging. Percentage deterioration of fabricated compositesalso demonstrated that hybrid composites performed better thannano and micro composites respectively.