Search or add a thesis

Advanced Search (Beta)
Home > Molecular Epidemiology of Clostridium Perfringens Isolated from Small Ruminants

Molecular Epidemiology of Clostridium Perfringens Isolated from Small Ruminants

Thesis Info

Access Option

External Link

Author

Mohiuddin, Mudassar

Program

PhD

Institute

Isra University

City

Islamabad

Province

Islamabad Campus

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/8861/1/Mudassar_Mohiuddin_Microbiology_HSR_2016_Isra_Univ_hyd_10.01.2017.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726700312

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


Introduction Clostridium perfringens presents persistent threat to the small animals in causing moderate to severe enterotoxemia. The pathogenicity of C. perfringens depends on the production of four major toxins. Therefore, typing of toxins elaborated by C. perfringens is essential. Specific toxin types are involved in causing particular enteric diseases in sheep and goats. Multiplex PCR has been introduced, which has the potential to detect the genes that code for these toxins. Also, the sensitivity and specificity of this method has been confirmed by amplification of specific target DNA under unique conditions. Therefore, the present project was designed to understand the molecular epidemiology of C. perfringens types isolated from sheep and goats. Objectives This study was carried out with following objectives: Ø To isolate, identify and characterize field strains of Clostridium perfringens isolated from sheep and goats in selected districts of Punjab, Pakistan. Ø To determine molecular epidemiology and heterogeneity of Clostridium species. Experimental Design It was an experimental study of two years duration, conducted at Multidisciplinary Research Laboratory, Isra University, Islamabad, Pakistan. vi Duration September 2013 to August 2015 Material & Methods Fecal samples were collected from healthy and diseased sheep and goats. Collected samples were cultured on cooked meat broth, blood agar and tryptose sulphite cycloserine agar. Biochemical characterization was carried out by using API 20A kits. Pure cultures of C. perfringens were used for DNA isolation by using spin column genomic DNA extraction kit. Isolated DNA was amplified by multiplex PCR with specific primers. Based on the length of the amplified fragments, bacterial strains were identified. The amplified DNA fragments were sequenced using Sanger dideoxy sequencing method. Alignment studies were carried out by using ClustalW, T-Coffee software. Results Results revealed that the major C. perfringens type among all healthy and diseased isolates was type A followed by type D. In addition to this, beta2 toxin was found in both healthy and diseased type A and D isolates. However the prevalence of beta2 toxin gene in diseased sheep and goat population was 64% as compared to 37% in healthy ones. The identified genes were found equally dispersed in both sheep and goat isolates. Nucleotide sequences of alpha, beta2 and epsilon gene revealed variations in the identified isolates. vii Conclusions It was concluded from this study that: Ø C. perfringens type A and D were prevalent in Punjab province of Pakistan while locally produced enterotoxemia vaccine did not include type A. Ø Nucleotide sequences of alpha, beta2 and epsilon gene revealed variations in the identified isolates which confirmed bacterial population heterogeneity. Ø Sequence analysis of the amplified cpb2 gene revealed two genetically different populations of the gene. Keywords Molecular epidemiology, C. perfringens, sheep, goats, multiplex PCR
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

صراطِ مستقیم

صراط مستقیم
ہم جب نماز اد کرنے کے لیے کھڑے ہوتے ہیں۔ فرض ادا کرنے ہوں ، وترادا کرنے ہوں ،نفل ادا کرنے ہوں ، واجب کی ادائیگی ہورہی ہے یا سنن ادا کرنے کی غرض سے حالت قیام میں سجدہ کی جگہ پر نظر جما کرمحوعبادت ہوں۔ ہر حال میں اور ہر رکعت میں باہوش وحوا س اہد ناالصراط المستقیم کی تکرار کرتے ہیں ، اس کے معنی یہ ہیں کہ ’’ اے اللہ ہمیں سیدھے رستے کی طرف ہدایت فرما‘‘ پابند صوم وصلوۃ شخص دن میں اڑتالیس مرتبہ بہ صدائے عجز و نیاز بلند کرتا ہے، اللہ تعالیٰ کے سامنے دعا کرتا ہے، ہر کلمہ گو شخص خواہ رنگ میں ،نسل میں ، نسب میں، حسب میں اختلاف ہی کیوں نہ رکھتا ہو طلب راہ راست میں برابر ہوتا ہے۔
اسلام دین فطرت ہے۔ یہ اپنے ماننے والے کے لیے ہر میدان میں ترقی وعروج کے دروازے وا کر دیتا ہے۔ اس کی تعلیمات پرعمل پیرا شخص صحت مند معاشرے کے ماتھے کا جھومر ہوتا ہے، ایساکبھی نہیں ہوتا کہ اللہ تعالیٰ سے دست سوال دراز کرنے والا شخص دراز قامت ہو ، کثیر الوجود ہو، کثیر العیال ہو، انعام و کرام سے نواز دیا جائے اور کوتاہ قد،نحیف، او ر قلیل العیال شخص بارگاہ ایزدی میں دست بدعا ہو اور وہ نامراد واپس لوٹ آئے۔ اللہ تعالیٰ کی رحمت ،نعمت اور انعام و کرام کے دروازے ہر ایک کے لئے ہیں۔
لیکن حصول رحمت ایزدی کے لیے سلیقہ مند ہونا انتہائی ناگزیر ہے اور سلیقہ بجز حُبِّ مصطفیٰ صلی اللہ علیہ و آلہٖ وسلم نہیں آ سکتا۔ کیونکہ اطاعت ِمصطفیٰ صلی اللہ علیہ و آلہٖ وسلم ہی اطاعتِ الٰہی ہے’’ جس نے رسول اللہ صلی اللہ علیہ و آلہٖ وسلم کی اطاعت کی اس نے اللہ تعالیٰ کی اطاعت کی‘‘ مناصب ِجلیلہ پر...

Human Organ Transplantation in the Light of Islam

Organ transplantation and organ donation are an urgent problem of our time. This was done in different ways. The medical field introduces new treatments, including organ transplantation and service to humanity. It is allowed to donate organs both in Judaism and Christianity. What is allowed in the Islamic perspective, transplantation and organ donation only if they follow the goals of Islamic sharia? The human body has the honor and dignity that must be preserved in every movement of life. In the case of organ transplants, if it leads to the destruct-tion of a human being and the violation of his honor or paves the way for the abduction, which offers immorality, and in these circumstances, it should not be done. Organ donation and transplantation will be beneficial, under the supervision of a qualified transplant team. In this article, the permissibility of organ transpla-ntation in the light of Islam has been discussed.  

The Influence of Seed Priming With P & Zn Solutions and its Optimization With Respect to Rate and Duration on Yield of Cereals

Benefits of seed priming can be best achieved if seed are primed in proper concentration of nutrient for optimum duration. A series of experiments were conducted in laboratory, greenhouse and field during 2008-2011 at The University of Agriculture, Peshawar to study the effect of P and Zn seed priming on germination, emergence and yield of cereals (wheat and maize). Laboratory experiments were conducted to determine the optimal duration and maximal concentration of zinc (Zn) and phosphorus (P) for priming wheat and maize seed. The seeds were soaked with water through a range of durations (0, 3, 6, 12, 18, 24, 30, 36, 42 and 48 h) and tested for germination. Seed soaked for 18 h resulted in higher mean germination and final germination whereas less time to 50% germination. In subsequent experiments, seeds were primed for 18 h in various concentrations of Zn (0.01%, 0.05%, 0.1%, 0.5%, 1%, 1.5%, 2.0%, 2.5%, 3.0% and 3.5%) and P (0.1%, 0.5%, 1%, 2%, 3% and 5%) along with dry and water soaked seeds as control treatment. Less time to 50% germination and higher mean and total germination was noted for seed primed with 0.1% and 0.5% P (wheat and maize); and 0.05% and 0.1% Zn (wheat) or 0.1% and 0.5% Zn solutions (maize). Chemical analysis of the Zn- and P-primed seed of wheat and maize revealed that increasing Zn and P concentration in the priming solution caused an increase in the Zn and P concentration of the primed seed, respectively. The two maximal concentrations of Zn and P were further evaluated in subsequent pot experiment in glasshouse. Results of the pot experiment revealed that lower time to 50% emergence and higher total emergence was recorded for seed primed in 0.05% Zn (wheat) or 0.1% Zn (maize) and 0.1% P (both crops). Seedlings resulted from seed primed with high concentration of P and Zn had more P and Zn, respectively. The field experiment consisted of three priming treatments i.e. NP (no primed seeds), ZnP ( wheat seed primed in 0.05% Zn and maize in 0.1% Zn) and PP (wheat and maize seed primed in 0.1% P); two levels of P (45 and 90 kg ha-1 for wheat whereas 30 and 60 kg ha-1 for maize) and two levels of Zn (10 and 15 kg ha-1 for both crops) as soil fertilization. Dry and water soaked seeds were used as control treatment. Urea and SSP were applied to provide N and P, respectively. Results showed that priming significantly affected emergence and yield parameters. Lower days to 50% emergence and higher emergence m-2, plant height, number of tillers m-2, number of spikes/cobs m-2, grains per spike/cob, thousand grain weight, grain yield and dry matter yield were recorded for seed primed in 0.05% Zn (wheat) or 0.1% Zn (maize) along with 90 kg P ha-1 (wheat) or 60 kg P ha-1 (maize) and 15 kg Zn ha-1 as soil application followed by seed primed in 0.1% P along with the same amount of fertilizer application. Phosphorus and zinc concentration in wheat and maize seedling, leaf and grain were significantly affected by different combination of priming and soil application of fertilizer. This simple, low-cost, low-risk and easily adoptable technology is expected to possibly reduce, to some extent, fertilizer and irrigation water requirements of the crop and significantly increase grain yield which will largely benefit economy of the resource-poor farmers and the country.