Search or add a thesis

Advanced Search (Beta)
Home > Nano-Antibiotics: Nano Encapsulation of Natural and Synthetic Antimicrobials to Combat Multi Drug Resistant Pathogens

Nano-Antibiotics: Nano Encapsulation of Natural and Synthetic Antimicrobials to Combat Multi Drug Resistant Pathogens

Thesis Info

Access Option

External Link

Author

Jamil, Bushra

Program

PhD

Institute

COMSATS University Islamabad

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/8108/1/Bushra_Jamil_Biosciences_2017_HSR_CIIT_26.05.2017.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726741782

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


Nano-antibiotics: Nano Encapsulation of Natural and Synthetic Antimicrobials to Combat Multi Drug Resistant Pathogens Increasing rates of bacterial resistance has invalidated the utility of even the most potent antibiotics, resulting in mortality due to failure in infection control and high health care costs. Therefore, design, discovery, and delivery of antimicrobial drugs with improved efficacy and avoidance of resistance are highly demanded. Use of nanotechnology in design and delivery of antimicrobial drugs, particularly in overcoming antibioticsresistant pathogens, is considered to be the most promising alternative for this purpose. The present study was designed with the aims to fabricate diverse bio-based nanoparticles (NP), which are non-toxic, biodegradable and biocompatible. Bio-based nanoparticles fabricated from soya lecithin (liposomes), chitosan and hybrid nanoparticles (a mixture of chitosan and β cyclodextrin) were developed and characterized. Natural antimicrobial agents (bacteriocin and essential oils) and synthetic antibiotics were encapsulated in these bio based nano-carrier systems. These nano-delivery systems were characterized by scanning electron microscope (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR) and Zeta sizer. It was observed that chitosan nano-particles and liposomes were in the size of less than 100 nm whereas the hybrid nano-particles were more than 300 nm in size. The stability of nanoparticles were measured from zeta potential it was concluded that chitosan nano-particles were bearing almost +50 mV zeta potential so were very stable whereas the stability of hybrid nano-particles and liposomes were considered to be compromised. In the first phase of experimentation cefazolin was encapsulated in above mentioned nano-systems and all these nano-systems were tested against multi drug resistant (MDR) Escherichia coli and Methicillin-resistant Staphylococcus aureus. Antibacterial studies confirmed that chitosan and hybrid nano-particles were effectively killing both MDR pathogens. Whereas, liposomes were not effective in killing them. xii However, chitosan was finally selected for further encapsulation of essential oils, nisin and other antibiotics. In the next phase, cefotaxime was encapsulated in chitosan to explore the antibacterial potential in depth by assessing colony forming unit (CFU) and anti-biofilm assay as well. It was determined that the bare chitosan nano-particles could control the growth of pathogens to maximum 48 hours and after that there was a sharp rise in optical density. Anti-biofilm activity confirmed that bare chitosan nano-particles inhibited the bacterial growth by forming agglomerates but they were not effectively combatting biofilm formation and that is the reason bacteria regrow after wards. However, in the case of drug encapsulated chitosan nano-particles the biofilm is totally eradicated. In the third phase, natural anti-microbial agents like essential oils and nisin were also encapsulated in chitosan and they were also found to be effective in killing the MDR pathogens. It is expected that the nano-encapsulation of antimicrobial agents will aid in control of resistance phenomenon in pathogenic microbes.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

شفیق الرحمن قدوائی

شفیق الرحمن قدوائی
شفیق الرحمن صاحب قدوائی اگرچہ آصف علی صاحب کی طرح آل انڈیا شہرت کے مالک نہیں تھے لیکن اس میں شبہ نہیں کہ نہایت مخلص قومی کارکن تھے۔ ملک کی تحریک آزادی کے سلسلہ میں وہ جیل بھی گئے، لیکن وہ طبعاً ہنگامہ پسند اور اسٹیج کے لیڈر نہیں تھے، خاموشی مگرنہایت مستعدی اورگرم جوشی کے ساتھ ٹھوس اور تعمیری کام کرنے کے عادی تھے۔انھیں سب سے زیادہ دلچسپی تعلیم بالغان کے کام سے تھی، اس سلسلہ میں انھوں نے جس بیدار مغزی،تن دہی اورقوت تنظیم و عملی سرگرمی کاثبوت دیا ہے وہ ان کی تاریخ حیات کاروشن باب ہے۔ اسی بنا پر یونیسکو کی طرف سے وہ انڈونیشیا میں تعلیم بالغان کے ناظم اعلیٰ بناکربھیجے گئے، وہاں سے واپسی پردہلی اسٹیٹ کے وزیر تعلیم منتخب ہوگئے۔ اس حیثیت سے ان کا ایک یادگار کارنامہ یہ ہے کہ اردو زبان دلّی کی علاقائی زبان تسلیم کرلی گئی لیکن غالباً ان کی آزاد طبیعت وزارت کی گراں بار ذمہ داریوں کی متحمل نہیں ہوسکی۔ اور وہ چند ماہ بیمار کر۵۲،۵۳ سال کی عمر میں ہی عالم جادوانی کوسدھار گئے۔نجی زندگی میں مرحوم بڑی خوبیوں کے انسان تھے۔نماز روزہ اور اسلامی شعائر کے پابند، نہایت سادہ اوربے تکلف، ہر ایک کے ساتھ ہمدردی اورخلوص کاتعلق رکھنے والے، بڑے ملنسار اورہنس مکھ، متواضع اور بامروت،ذہین اورمعتدل المزاج۔ حق تعالیٰ ان پر اپنے انواروبرکات کانزول فرمائے اور جنت الفردوس میں ان کو مقام عطافرمائے۔ [مئی۱۹۵۳ء]

 

سیرت طیبہ صلی اللہ علیہ وآلہ وسلم کا عدلیاتی پہلو: ایک تحقیقی جائزہ A Research Review of the Prophetic Justiceﷺ

Islam is a religion of peace and it has provided a strong system of justice for the establishment of peace and order, the practical picture of which is available to the Muslim Ummah in the form of Sira-e-Taiba of the Holy Prophetﷺ. On which the seal of beauty has been recorded by Allah Himself. Therefore, the system of justice of Islam can be used only when the solution of all issues is sought from Sira-e-Taiba because Heﷺ has eliminated the distinction between rich and poor in the supremacy of law. The Holy Prophet ﷺ has set great and enlightening examples of justice for humanity, the pursuit of which leads to peace and tranquility of social life. In his Supreme Court, the Qur'an and Sunnah and consensus and analogy were of fundamental importance. In the Qur'an and Hadith, Sira-e-Taiba had the status of Shāri' and Legislator, while in consensus and speculation, Sira-e-Taiba has promoted thinking and counseling. The center and axis of Islamic law is your caste. He ﷺ has established justice, supremacy, and law in every field of worship, affairs, disputes, and crime. Therefore, in this article, the judicial aspect of His ﷺ Sira-e-Taiba has been explained in the light of the Qur'an and Hadith, the Sahabah and the followers, and the edicts of the Imams and jurists.

Efficient Utilization of Bandwidth for Ofdm Wsn

Wireless Sensor Networks (WSNs) have gained popularity in a lot of emerging areas since their evolution, like the monitoring of natural phenomena concerning geosciences in various disciplines. Some relevant areas include the monitoring of oil and gas exploration field, earth quake and volcanic eruptions etc. In these processes real time phenomena are monitored in remote fields and after data collection through WSN, it is ported to the far flung research centers for further investigation and decision making. Here 2-tier WSN is considered with lower tier of sensor nodes in WiFi and upper tier of WiMAX as a backhaul for data transportation to distant research facility. However WiMAX has already popularity for applications with main configuration of downlink data delivery direction, like serving internet hot spots and similar amenities. This innovative research work is concerned with previously mentioned phenomena monitoring which needs high data throughput efficiency in the uplink direction. For dense sensor node concentration, in order to transport consolidated output form all sensor nodes in real time phenomena of impulsive nature, sufficient uplink throughput is needed with low latency which forms a bottleneck in these cases for WiMAX backhaul. Real time applications constrain end-to-end delay and hence throughput which severely affect the performance and the accuracy of the monitoring. To the system only fixed bandwidth is available, for which resources of slots have to be shared between downlink and uplink. Here WiMAX OFDM in TDD mode is considered. Initially analysis has been presented to point out main important constituent parameters of WiMAX which contribute to throughput. This research work proposes a solution to enhance uplink bandwidth allocation efficiency for these phenomena through adaptive shift of WiMAX frame ratio. In this regard for WiMAX adaptive ratio shift researchers have used a number of scenarios but all of them are not very efficient and have drawbacks. Some of them are using either fixed margins or some other are using fixed step sizes for upward or downward (increment) without any calculation. If a link (uplink or downlink) is running near full capacity then any kind of sudden appearance of internal system overheads in the form of system message or broadcast may bring the link to fragmentation. And fragmentation successively may contribute to increase the link overheads further, causing either increased delay or some other problem related to packet drop, re-transmission or transmission failure. If problems are induced, this may take relatively longer at a reduced data transfer rate. In order to avoid similar problems, relevant calculations have to be performed to guarantee good QoS values of maximum link throughput while keeping low delay and packet drop, which needs adequately more resources. In fact keeping in view the complex trade-off between QoS parameters and system resources, the optimization problem is formulized to maximize the uplink throughput while keeping the latency and packet drop of downlink to minimum limit, to facilitate the efficient operation of uplink’s momentarily bulky traffic. A novel solution to this problem is obtained through the incorporation of stochastic processes with random variable in finite state space. The analytical and mathematical expressions are contributed for the different analytical models. A MMPP traffic model is formed for OFDMA transmission. This is supported with a DTMC system model for queuing theoretic performance modeling. Analytical and numerical values of performance parameters like throughput, packet delay and probability of packet drop are estimated for resource allocation through mathematical models of stochastic process. First of all to restrict the downlink traffic to minimum level a traffic pattern is defined from downlink to WSN sensor nodes. While considering packet scheduling, two scenarios are taken into account, one is concerned with known MCS distribution and the other is concerned with unknown MCS distribution. In this process mainly affecting factors of frame overheads are also taken into account. Then by using all these analytic and mathematical models, an algorithm is formulated to find out minimum optimal resource requirement on down link after considering these QoS parameters. Through this manipulation rest of the resources can be transferred to facilitate uplink operation. Another DTMC model is designed to check and quantify the uplink frame utilization. When uplink frame utilization is beyond normal, the frame resources have to be incremented to previously calculated maximum limit by adaptively remapping the frame ratio. Also when utilization on uplink reduces below normal, it is remapped to normal frame ratio. The main algorithm is formed by appropriately including all these previous analytical models. This sets the adaptive ratio to the best suitable maximum value, to facilitate the uplink bulky traffic, and saves the link from congestion and slowing down. This also ensures minimization of previously mentioned errors of delay, drop or timeout related problems. This process achieves highest degree of convergence in just one step by providing maximum throughput on uplink without degrading QoS parameters on the downlink operation. In order to prove the results firstly analytical results are obtained from the computations of the algorithm in MATLAB. Secondly intensive simulations are conducted around Rayleigh flat fading channel through models in three steps. One simulation model gives results for downlink minimum traffic, next model is for uplink maximum traffic and another model is for uplink normal ratio traffic. All these results together prove the accuracy and superiority of the algorithm by showing an outstanding uplink bandwidth efficiency enhancement without degrading the downlink operation. Future research directions are to further enhance the analytical models for more states. Also more variations to overhead analysis can be added by more realistic models and with more overhead reduction techniques.