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Nanoparticles-Based Sensing Strategies for Detection of Bacteria in Water

Thesis Info

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Author

Mumtaz, Shazia

Program

PhD

Institute

Lahore University of Management Sciences

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12921/1/Shazia_Mumtaz_Chemistry_HSR_2018_LUMS_lahore_24.08.2018.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726742416

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The present study was designed to develop facile, reproducible and economically viable bacterial sensing assays using functionalized metal/metal oxide nanoparticles/nanoclusters. For this purpose, iron oxide and mixed ferrite nanoparticles functionalized with dopamine were produced and evaluated for their peroxidase mimicking ability. Dopamine capped iron oxide (DOPA) nanoparticles were then utilized to develop antibody-free as well as enzymefree colorimetric assay for the sensing of Escherichia coli (E.coli) and Bacillus subtilis (B.sub) with detection limit as 104 cfu/mL by naked eye and 102 cfu/mL by spectroscopic measurements respectively. The 2-(3-(3,4-dihydroxyphenyl)propanamide)-N,N,Ntrimethylethanaminium chloride capped MnFe2O4 nanoparticles (DPTMA- MnFe2O4) were integrated into the electrochemical sensor as both transducer and recognition element to develop bio-label as well as bio-recognition element free sensing methodology with differentiation limit of bacteria down to 102 cfu/mL. In yet another effort, blue fluorescent polyethylenimine coated silver nanoclusters (PEI-AgNCs) were evaluated for the detection of bacteria in solution. The fluorescent intensity of nanoclusters was gradually decreased in the presence of bacteria resulting in differentiation of bacteria up to limit of 104 cfu/mL.
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مولانا محمد اعزاز علی

آہ! الا ستاذ الاجل
۸؍مارچ کے اخبار الجمعیۃ میں جب یہ خبر نظر سے گزری کہ حضرت الاستاذ مولانا محمد اعزازعلی صاحب پرقلب کادورہ پڑگیا اوراس کی وجہ سے کچھ بے ہوشی رہی اوراب تھوڑی تھوڑی دیرکے بعد دورے پڑرہے ہیں تواسی وقت ماتھا ٹھنکا کہ خدا خیرکرے۔چٹان جب گرتی ہے تو مٹی کے تودہ کی طرح رِس رِس کے نہیں اچانک ہی گرتی ہے۔چنانچہ دوسرے دن کااخبار آیاتو دل کے دغدغہ کی تصدیق ہوگئی اور جس خبرِوحشت اثر کوسننے کے لیے کان تیار نہ تھے اس کایقین کرنا پڑا۔ یعنی حضرت الاستاذ راہی ملک بقا ہوگئے۔اِنّا لِلّٰہِ وَاِنَّا اِلَیْہِ رَاجِعُوْنَ۔
دارالعلوم دیوبند شروع سے معدنِ لعل وگہر رہا ہے۔کتنے ہی ذرے اس کی آغوش میں پلے اور بڑھے اورعلم وفضل کے آسمان پرآفتاب بن کر چمکے، کتنے چاند اورستارے اس کے آسمان پرطلوع ہوئے اوراپنی اپنی روشنی دکھا کراسی دارالعلوم کے دامن میں روپوش ہوگئے، کیسے کیسے گُہر ہائے آبدار اس کی خاکِ پاک سے اٹھے اورعلم وعمل، تقوی وطہارت اورزہد و ورع کی بزم قدس کوجگمگاکر پھرخاکِ لحد میں جاملے ۔آج وہ نہیں ہیں لیکن ان کی یادگاریں باقی ہیں خودان کا وجود فنا ہوگیا لیکن ان کے کارنامے زندہ ہیں اور وہ گویا خود زبانِ حال سے کہہ رہے ہیں:
تلک آثارنا تدل علینا

فانظر وا بعدنا الی الآثار
دارالعلوم دیوبند اگر شاندار عمارتوں ،درسگاہوں،اقامت خانوں اور وسیع و فراخ دروازوں اور اونچی اونچی دیواروں کانام نہیں بلکہ درحقیقت وہ انھیں نفوسِ قدسیہ کاایک پیکرِ محسوس اور انھیں ارواحِ طیبہ کاایک مظہرِ مادی وجسمانی ہے تو کوئی شبہ نہیں کہ حضرت الاستاذ اس عمارت کے ایک اہم ستون اوراس بزمِ انس و قدس کے ایک لعلِ شب چراغ تھے۔گزشتہ نصف صدی میں اس درسگاہ کوتعلیم و تعلم کے اعتبارسے جوشہرت وعظمت حاصل رہی ہے اس میں ایک بڑا حصہ...

اسلام اور دوسرے مذاہب میں خدا کے تصور کا تحقیقی و تقابلی جائزہ

The word “God” and its derivatives apparently seen common knowledge. However when the concept of God is studied through various religions, it becomes evident that this word has undergone a prolonged evolutionary stages. Therefore, it may safely be said that this vital concept has evolved and later undergoing through various transformation culminated on the concept of “Tawheed”. This article attempts to present an overview of the concept of God with reference to different religions as this concept of God has remained a focus of man’s activities.    

The Study of Shortcomings in Conventional Banking System: A Maqasid-E-Shariah Perspective

It is unanimous quote that the life blood of development is the sound economy of a country. The trend reveals the fact, all the developed countries across globe are symbols of cited above quote. Contrary to the said saying, countries deficient of it are still striving for survival and the world ranks them in the list of under developed countries. It is also pertinent to mention that along with other relevant factors to boost up the economy, banking sector play a very keen role in providing the smooth ground one way or other. The fact beyond it is quite clear as this Industry has depth involvement in almost all life businesses. Nobody can deny the importance of this sector that is why the fast growth has been observed in Banking Industry so for as comparative to other economic sectors. Similarly, the financial history depicts the datum about gray aspect of this sector. The downfall of financial industry leads towards the collapsing of entire economy as almost all the core economic activities based on this industry directly or indirectly. In last centuries, financial crises has been observed numerous time affecting the global economies and put the adverse impact on routine economic cycles for long time. The economist played a vital role in evaluating these financial crises and presented thousands of research papers and reports aimed to explain the issue and causes of its occurrences. Likewise different surveys has been conducted in order to get core and real causes of these disasters but all in vain. After practicing a large number of remedial measures and opting thousands of suggestion in this regard results almost same consequences. The world economy faced huge financial disaster on average after decade or two like Wall Street Crash of 1929 and Great Depression, Oil Crises in 1973, Latin American Debt Crises in 1982, Black Monday in1987, U.S Saving and Loan Crises in 1989, Japanese Asset Bubble in 1990, Mexican Peso Crises in 1994, Asian Financial Crises in 1997, Russian Financial Crises in 1998 and Dot-Com bubble in 2000. The worst case occurred in 2008 that affect the world economy severely. Almost all the dollar base countries felt the shocks of these crises. The summary of almost all recommendations towards its healing and to bringing them in a streamline of rationality shows that all are primarily made on derived assumptions of mankind and based theoretical phenomena’s constantly leaving the space for individual’s curiosity and interest which leads the system to repeat itself in year or decade in the shape of new financial disaster or financial crises. Important to mention that after the use of bulk of energies and utilization of all type resources, why the economist are unable to explore the solid and firm system to accomplish the financial objectives of humanity and why the world used to face such disaster in a cyclic manner and why the financiers remains in risk of “anything may happen anytime” and in fear of uncertainty. The only answer to all raised question is very clear that we always construct our system on man-made policies and procedures rather than opting the divine rules and orders. “An interesting irony that every dollar note has the admission: “In God we trust”, but when it comes to develop theories to earn dollars or to distribute or spend them, trust is placed only on human ideas based on personal assessments; God is held totally out of picture, as being irrelevant to economic activities’. [Taqi Usmani, 2009].This is the quandary that high financial experts and economist didn’t realize and nobody confer attention towards it to know the root cause of downfall. Manmade theories are always limited to specified era and restricted to particular setting. That is why the world economy consistently running with high risk. So there is dire need felt to observe the banking system in the light of divine rules to ensure its smooth operations with full confidence of sound and firm procedures from customer to organizations along with reliance of all stakeholders operating in the said system directly or indirectly. This research study will mainly covers the identification of shortcomings in conventional banking system with respect to sharia’s principles and laws only. Those products, trends, policies and dealings of it violating the cultural norms, international accounting or auditing standards and any other sort of disrespect and lacking is not the considerable part of this research. Along with that, it also focus on describing the remedial measures of the loopholes in order to eliminate them from the system to ensure the smooth flow of financial operations and to protect it from adverse violations and exploitations. The study will present the some in vogue practices of Islamic banking system in the form of portraying the detail discussion of mostly used modes of financing like Mudarabah, Murābaḥa, Ijarah, and Diminishing Musharakah along with their procedural documentations and patterns exist in the Islamic Banks as sample that how these modes are free from all such lacking and weakness what the conventional Banks have which is the need of the day to address properly in order to bring the entire system in full conformity of divine standards and laws. For the said purpose, the study includes all the sources of Shariah i.e. Qura’n, Sunnah, Ijma and Qias classical literature and contemporary financial reports, surveys and procedural laws of different financial Institutions special emphases on conventional Banks. On the basis of findings of the study, the suggestions will be stated to bring the deviations in accord with the mainstream Sharia.