تفسیر جلالین میں اسرائیلی روایات کا تجزیاتی مطالعہ

Holy Quran is the most sacred scripture bestowed by Allah Almighty upon his bellowed prophet Muhammad (PBUH). Unlike other scriptures, Allah Almighty himself took the responsibility of safety and safe custody of the Holy Quran. That is why, despite passage of more than 1400 years, the Holy Quran remained intact, wordwise as well as meaningwise and will remain intact till the end of the world. Such a unique status of the Holy Quran could not be tolerated by the enemies of Islam and Prophet of Islam. Therefore, they decided to present Holy Quran as a defective document before its readers. For this purpose, they chose to highlight and exploit the short and abbreviated incidents, described by the Holy Quran, under the term of “Muthashalihat”, by adding and narrating false, nonsense and most astonishing stories, just to divert attention of the readers from the basic learning of Holy Quran, towards un-realistic and bogus stories. Accordingly majority of the readers have shown great interest in these stories, which fall under the title of Israiliat Unfortunately, the said stories are included in dozens of Translations Tafaseer of the Holy Quran. In order to acquaint readers of the actual status of Israiliat, the undersigned has started working on the subject. Since Tafseer-e-Jalalain is included in the Syllabus of Maddaris and being taught for the last many years, therefore, I have chosen the same in the first instance, and pray Allah Almighty to help me out in completing my assignment.

Molecular Diagnosis and Prevalence of Brucellosis in N. W. F. P Pakistan

Brucellosis is a disease with major social and financial implications in many countries, particularly in the developing world. In Pakistan, although there are systems for the detection of brucellosis based on serum agglutination testing, more sophisticated methods such as Polymerase Chain Reaction (PCR), Real-Time PCR, Enzyme Linked Immunosorbent Assay (ELISA) and Fluorescence Polarization Assay (FPA) are not applied routinely. Similarly, the classical and particularly molecular characterization approaches to understand the epidemiological features of Brucella are in infancy. In the present study, serum was used as clinical specimen for serological and molecular diagnosis of brucellosis whereas field culture isolates were used for conventional and molecular characterization purposes. Milk samples were also tested where possible. In total, 277 serum samples of both human and animal origin were collected from 6 districts of Khyber Pakhtunkhwa Province of Pakistan. These samples were then tested at the Animal Health and Veterinary Laboratories Agency, United Kingdom for further validation. From 167 animal sera, 4%, 6%, 13%, 21% and 31% animals were positive by RBPT, cELISA, PCR BCSP31, PCR IS711 and Real-Time PCR, respectively. Out of total 110 human sera belonging to various occupational groups, 5%, 9%, 31%, 38%, 53% and 60% were detected positive by RBPT, cELISA, FPA, PCR BCSP31, PCR IS711 and Real-Time PCR, respectively. A total of 51 milk samples were tested which revealed the prevalence of brucellosis as 12% with Indirect ELISA and none of the sample produced positive results by Milk Ring Test (MRT). In human, positive male showed clinical complaints of arthritis and hepatitis while in female abortion in 4-5 month of pregnancy, headache and arthritis were the main clinical signs. Amongst the positive detected animals, abortion in last trimester, retention of placenta and mastitis were the key clinical features. Cultured field isolates when subjected to phenotypic and molecular characterization techniques found to be in resemblance with Brucella melitensis biovar1. These results give an indication of the prevalence of brucellosis in Pakistan and show that the disease is not only confined to livestock but is also present in the human population. In Pakistan, where there are few centres for brucellosis testing and large areas to cover, serum might provide the best specimen for the determination of infection, based on its stability over time.