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Home > Neurochemical & Behavioral Effects of Non Steroidal Antinflammatory Drugs Nsaids in Animal Model of Learned Helplessness.

Neurochemical & Behavioral Effects of Non Steroidal Antinflammatory Drugs Nsaids in Animal Model of Learned Helplessness.

Thesis Info

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External Link

Author

Emad, Shaista

Program

PhD

Institute

University of Karachi

City

Karachi

Province

Sindh

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Biochemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/10335/1/Shaista%20Emad_BioChem_2017_UoK_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726746863

Similar


Stress and inflammation are mutually linked processes that encompass many pathological conditions. Inflammation is a body‟s defensive mechanism which is activated in response to tissue injury or infection with the release of inflammatory mediators. Studies on animal models provide useful information regarding psychological and physiological affliction leading to modifications in behavioral, biochemical and neurochemical changes. Stressful events in life often precipitate depressive behaviors, dysfunction metabolic pathways and triggers metabolic syndromes. Stress also encourage generation of oxidative species and reduced activities of endogenous enzyme system, prompts neuronal damages and concomitant development of neurodegenerative diseases. Similarly changes in the neurotransmission system more specifically serotonergic and dopaminergic system have implications on variety of functions ranging from impaired motor coordination, memory deficits, aggression and anhedonia.The present work highlighted effects of stress responses in modulating inflammation when rats were subjected to different psychological and physical stress paradigms such as 2h restraint stress, repeated restraint (2h/day for 5 days) stress and dissimilar stress. Antiinflammatory approaches by the use of non selective NSAIDs (indomethacin and diclofenac sodium) were used to identify possible mechanism of actions of these drugs in the reduction of stress induced inflammatory responses and treatment of stress associated neurological disorders in animal models of stress. Important findings of the thesis are as per the following: 1. The primary finding of present study revealed that repeated administration of indomethacin and diclofenac sodium improved behavioral functions in rats suggesting neuromodulatory effects of NSAIDs on central nervous system. 2. Exposure to 2h restraint stress causes behavioral deficits in the form of reduced exploratory activity, anxiogenic and depressive behaviors in rats. Whereas, augmented memory functions were observed after 2h restraint stress. Pretreatment xxiv with indomethacin and diclofenac sodium was found to normalized 2h restraint stress related alterations in rats when compared with unrestrained rats. 3. Following repeated restraint (2h/day for 5 days) stress both adaptive and maladaptive changes were observed in rats. The adaptive responses as observed on exploratory activity and anxiety states were comparable in restrained rats as compared to unrestrained rats. However, the maladaptive outcomes due to repeated restraint stress were observed in the form of depressive behaviors and decline in memory functions. Indomethacin and diclofenac sodium improved behaviors in rats exposed to repeated restraint stress. 4. Rats exposed to dissimilar stress showed marked behavioral deficits. Continuous exposure to a variety of stressors also served as a model of depression in animals. Indomethacin and diclofenac sodium showed improvement in behaviors. This suggests the role of NSAIDs as a potential therapeutic agent against a variety of neurological disorders. 5. An episode of 2h restraint stress, repeated restraint (2h/day for 5 days) stress and dissimilar stress showed alterations in corticosterone, glucose and cholesterol levels. Our results indicate that these biochemical changes were successfully attenuated by the administration of indomethacin and diclofenac sodium. 6. The study was further aimed to investigate the effect of 2h restraint, repeated restraint (2h/day for 5 days) and dissimilar stress exposure on the oxidative status and also on the activities of antioxidant enzymes (SOD,CAT and GPx) in rat‟s brain. Both indomethacin and diclofenac sodium decreased lipid peroxidation and enhanced functions of endogenous antioxidant enzymes system. This suggests antioxidant properties of these drugs against a variety of stressors. 7. In present study acetylcholinesterase enzyme activity on exposure to 2h restraint stress, repeated restraint (2h/day for 5 days) stress and dissimilar stress showed changes in cholinergic functions in rats. Indomethacin and diclofenac sodium was xxv found to inhibit activity of AChE enzyme similar to those of AChE inhibitors and hence improved memory functions of rats in stress conditions. 8. The study also revealed effect of 2h restraint stress, repeated restraint (2h/day for 5 days) stress and dissimilar stress situations on neurotransmitters levels in rats. Variable results were observed such as increased or decreased serotonergic or dopaminergic activities on exposure to various stress situations. The changes in neurotransmitters also reflected impairments in behavioral activities in rats. Indomethacin and diclofenac sodium was found to normalize the disturbed activities of neurotransmitters in rats. The study tends to suggest that non steroidal antiinflammatory drugs might be used for several stress related pathological features of the central nervous system (CNS). As these drugs have an overall positive outcomes as observed in present thesis by improving the general behaviors, reducing oxidative stress and increasing the levels of serotonergic and dopaminergic neurotransmitters in brain and could be effective to prevent neurological changes accompanied with stress.
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مولاناعبدالماجد بدایونی

مولانا عبدالماجد بدایونی
افسوس ہے کہ اس سال کا خاتمہ بھی ماتم پر ہوتا ہے، خطیب الامت مولانا عبدالماجد بدایونی رحمۃ اﷲ علیہ کا ناگہانی سانحۂ ارتحال ہمارے لئے ذاتی اور قومی دونوں حیثیتوں سے وہ غم ہے جو بھلائے نہیں بھولتا۔
۱۴؍ دسمبر ۱۹۳۱؁ء کی نصف شب کو یہ واقعہ لکھنؤ صدر میں پیش آیا تو میں وہاں اس صبح کو موجود تھا، ۸ بجے صبح کو خبر ہوئی جب ۹ بجے کے بعد وہاں پہنچا تو مرحوم کی زندہ روح خدا کے پاس اور مردہ لاش بدایوں کو منتقل ہوچکی تھی۔
مولانا عبدالماجد بدایونی کون تھے؟ لکھنے والے ان کے محامد و اوصاف صفحوں میں لکھیں گے اور بیان کرنے والے گھنٹوں بیان کریں گے، لیکن اس سارے دفتر کو صرف ایک لفظ میں اگر ادا کرنا چاہیں تو کہہ سکتے کہ وہ ہستی جو سرتاپا محبت تھی، خدا سے محبت رسول سے محبت، آل رسول سے محبت، اکابر سے محبت، دوستوں سے محبت، کارکنوں سے محبت، عزیزوں سے محبت۔
حضرات علماء کے طبقہ میں ان کی ذات ہر حیثیت سے قابل فخر تھی، ان تمام لوگوں پر جنھوں نے طرابلس کے زمانہ سے اسلامی جدوجہد میں شرکت کی، ان بیس برسوں میں مختلف دور گزرے، یعنی کچھ آرام و سکون، پھر کچھ سعی و محنت، کچھ عزلت گزینی اور پھر ہنگامہ آرائی، کچھ توقف، پھر تیزرفتاری، اس طرح ان کی زندگی کے ایام وقتاًفوقتاً گزرتے رہے، مگر جماعت علماء میں یہی ایک ہستی تھی جس کی زندگی کے ایک لمحہ کو بھی اس وقت سے چین نصیب نہ ہوا، ہر وقت و ہر نفس ان کو کام کی ایک دھن لگی ہوئی تھی، جس کے پیچھے ان کا آرام چین خانگی سکون، اہل و عیال اور جان و مال ہر چیز قربان تھی، یہ بھی سماں گزرا ہے کہ ان کے گھر میں کفن دفن کا...

Admissibility of a Video As Evidence in a Court: its Presentation and Probative Value

To general public, all videos are perceived to be true, but they may not have probative value in the Court of law. The undertaken article analyzes the admissibility and probative value of a video presented as evidence before a court in the Criminal Justice System of Pakistan (CJSP). It analyzes the relevant law and diagnoses the problems with the video evidence through the lens of the judgments of Superior Courts. The court of law objectively ascertains that a video presented as evidentiary means bears significant relevance to the fact in question. It must be admissible under the law, and it must be proved to be genuine. To fill up the gap between a “Video” and a “Video Evidence”, there is a process, which is known as video authentication. It determines that the video contents are genuine, authentic, credible, unaltered, untampered and unfabricated. The study discusses various modes of video authentication. Precedents set by superior courts of Pakistan show that convictions have been made once the courts are satisfied with the credibility of video evidence. In the court of law, video evidence is normally presented after the completion of prosecution evidence. The video is played in court and is watched by the presence. But the researcher establishes that such process does not have legal justification. The article suggests that it would be legal and proper for the prosecution to produce the video evidence through the witness, during his evidence, who is either victim, witness, recorded and/or copied the video directly from original source such as C.C.T.V system and that witness would be subjected to cross examination.

Effect of Chemically Treated Wood Coal on the Physiochemical Properties of Tomato Riogrande

In a pot experiment, wood coal was applied to tomato plants of Rio Grande cultivar in the form of 32 different treatments, which were made by treating the same number of powdered wood coal portions (250g each ) with one liter each of 32 different acid base solutions i.e. 4% HCl, 4% H2SO4, 15% HNO3, 5% NaOH, 5% KOH abbreviated as ''C'', ''F'',''N'', ''S'', ''P'' and their combinations CF, CN, CS, CP, FN, FS, FP, NS, NP, SP, CFN, CFS, CFP, FNS, FNP, FSP, CNS, CNP, CSP, FSP, NSP, CFNS, CFNP, CFSP, CNSP, FNSP, CFNSP in one ratio one respectively. Quality of the treated wood coal and its role as fertilizer was studied in term of physicochemical parameters of pots soil and tomato plants in comparison to controls i.e. NPK, humic acid, F.Y.M, wood coal and untreated soil. pH and EC of the treated wood coal showed significant difference (P<0.05) with ranges of 1.48 to 11.9 and 3.45 to 28.4µs/dl respectively, where most of pH values were in acidic region, except S, P, CS, FP, SP, CSP and FSP treatments while EC was highest in acidic or acid containing treatments. Soil parameters showed significant variation (P<0.05) where the least EC 96.60 µS/dl was found in untreated soil while highest 190.10 µS/dl in NSP treated soil.Acidity of soil ranged from 0.97x10-2g/100mL in S to 1.41x10-2g/100mL in FP treated soils. The highest value 6.58x10-3% of inorganic nitrogen was observed in CFNSP treated soil. An increase in soil pH from 7.85 to 8.61 was observed after treatments where treatment wise comparison showed a range from 8.10 to 8.32 in CFP and CNS respectively. The effect of treatments on soil minerals in mg/kg indicated greatest variation in Fe from 2.759 to 5.358, Mn from 0.324 to 0.73, Na from 3.9 to 11.9 and K from 8.60 to 15.30 while other minerals like Cu, Zn, P, Pb and Ni showed moderate variability. Higher trend of soil minerals were found in sulfuric or nitric acids containing treatments. Data of tomato plants physical parameters,taken in intervals each of 15 days, varied significantly (P<0.05) where higher values of plant height 51.53cm was found in CN, leaf area 26.77 cm2 in FSP, No. of leaves plant-113.13 in CFN, No. of leaflets plant-1 53.93 in NP and No. of flower plant-1week-1 13.80 in SP treated plants with lower values of these parameters25.20 cm, 15.94cm2, 8.07, 33.87 and 1.42 in untreated soil respectively, while No. of branches 4.56 of CNP were higher than humic acid 1.56 and No. of fruits plant-1week-1 4.75 of FNS than 1.00 of CSP. Proximate composition of plant parts taken in percent showed the higher values of moisture 96.28, crude protein 1.30 and NFE 88.36 in roots, ash 4.75 and crude fiber 7.32 in stem and crude fat 2.64 in young leaves. Percent proximate composition of treated plants varied from 0.5 to 3, where maximum values ofmoisture 95.67, ash 4.03, crude fat 2.05, crude protein 1.21, crude fiber 6.05 and NFE 87.94 were detected in C, CFP CNS, CNSP, CFNP and FN treated plants respectively. Except Na and K with highest values of 16.10 and 37.63, other minerals of plant parts, like Fe, Zn, Mn, P etc., varied from 0.01 to 3.00mgKg-1 where most of them were higher in roots and leaves of tomato. Treatment variation of these mineral were also significant at P=0.05 where highest values of Fe was found in CNSP, Zn, Cu and in C, Mn in FNSP, Na and P in NSP, K in N and Ni was highest in FSP treated plants. Most of the fruits proximate parameters except moisture 94.82% and NFE 96.88% ranged from 0.02 to 3% with higher values in KOH containing treatments. The lowest values of these minerals were present in untreated soils. Among the mineral content of fruits Fe content of FNSP, Zn of CFSP, Cu and Na of CFNS, Mn and P of CS, Pb of CNP, Ni of SP were highest compared to other treatments. Highest values of Carbon 84.16% and lycophene 4.50mgKg-1 were found in CFP and FSP treatments, while chlorophyll was high at day 45of transplantation and in CFNSP treated plant. From the results it was conjectured that treated wood coal may be developed as fertilizer for tomato and other crops where further research particularly under field condition are needed.