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Home > Neuropharmacological Studies on Memory Enhancing and Reinforcing Effects of Cns Stimulants in Rats Exposed to Unpredictable Repeated Stress

Neuropharmacological Studies on Memory Enhancing and Reinforcing Effects of Cns Stimulants in Rats Exposed to Unpredictable Repeated Stress

Thesis Info

Access Option

External Link

Author

Kanwal, Sumera

Program

PhD

Institute

University of Karachi

City

Karachi

Province

Sindh

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Biochemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/13534/1/Sumera_Kanwal_Biochemistry_2016_UOK_Karachi.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726749426

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There is growing increase in the prevalence of depression and obesity. In addition there is growing need of cognitive enhancers. CNS stimulants seem to be effective alternative for all these therapies but are addictive. Apomoprhine, a D1/D2 agonist also produces CNS stimulant like effects. The present study is designed to monitor antidepressant and cognitive improving effects of apomorphine, methylphenidate and caffeine in rats exposed to unpredictable repeated stress. Antidepressant like effects was monitored as the ability of drug to reduce stress effects on locomotion in a familiar as well as novel environment. Reinforcing effects of drug were monitored as drug-induced behavioral sensitization and place preference in a CPP paradigm. Cognitive performance and drug-induced enhancement of learning and memory were monitored in a Morris Water Maze Test. Apomoprhine, methylphenidate and caffeine exhibit antidepressant like effects by producing hyperlocomtion and anorexia. Long term administration of moderate dose of apomorphine and low doses of methylphenidate and coffee produces sensitization and enhances CPP greater in stressed animals. However when apomorphine is experienced during the exposure of unpredictable repeated stress it suppresses stress effects and enhances locomotion, drug induced sensitization was also smaller. Single as well as repeated administration of low dose of methylphenidate enhances short term and long term memory whereas high dose enhances memory retention and produces sensitization and reinforcement. Coffee is also effective in reducing stress-induced cognitive impairment, but reinforcing when taken at high dose specially for stressful conditions. The present study shows that CNS stimulants such as methylphenidate and caffeine can reduce stress effects and may be useful for the treatment of depression. Apomorphine which also produces CNS stimulant like effects is also similarly effective. Moreover the present results tend to suggest that psychostimulant as well as apomorphine can improve cognitive performance and alleviate stress-induced memory impairment. Psychostimulant as well as apomorphine have addictive potential and should not be used for long term.
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نسیم سخن اور صاحبِ کتاب

’’نسیم سخن‘‘ اور صاحبِ کتاب
حافظ محمد اکرم راشدؔ سے میرے دیرینہ اور دیر پا تعلقات ہیں ، یہ ایک علمی خاندان سے تعلق رکھتے ہیں۔ اِن کے بزرگوں کے دینِ اسلام کے میدان میں لگائے ہوئے شجر سایہ دار مسحور کن ماحول پیش کر رہے ہیں اور تشنگانِ علم کی پیاس بجھانے کے لیے دورانِ سفر طلباء کے راستے میں آنے والی جہالت اور کم علمی کی تپش اور دھوپ کو رفع کرنے میں مثالی کردار ادا کر رہے ہیں ۔ میں نے صرف کتاب اور صاحبِ کتاب کے بارے میں چند سطور ضبط تحریر میں لانے کے لیے اپنے قلم کو اذنِ خرام دینا ہے ۔موصوف قلم کے میدان کے شاہسوار ہیں ، ندائے حق کی ادارت ہو، منظور العارفین کی تدوین ہو ، یا منظورالعارفین ٹرسٹ کا قیام ہو، موصوف پیش پیش نظر آتے ہیں ،آپ ہمارے ادارے منظور العارفین ٹرسٹ کے ساتھ قلب و اذہان کی جملہ قویٰ کے ساتھ وابستہ رہے ہیں ۔ مُرور ایّام کے ساتھ پیرانہ سالی اور ضعف کا شکار ہو کر کچھ عرصہ سے گوشہ نشین ہیں تاہم تحریر سے عشق کی حد تک لگائو کی بنا پر کوئی نہ کوئی شاہ پارہ تخلیق کرتے رہتے ہیں ۔ آپ کالم نویس ہونے کے ساتھ ساتھ ایک عظیم خوش نویس بھی ہیں عارفوالااور اس کے مضافات میں خطاطی کے حوالے سے ان کا ایک نام ہے ۔ دنیوی اور دینی تعلیم کے امتزاج کے حامل ہیں اور اپنی ایک شناخت رکھتے ہیں ۔ سرکاری ادارہ میں رئیس مدرسہ کے فرائض سر انجام دے چکے ہیں ۔
مذہبی خدمات کے حوالے سے اِن کی خدمات مہر نیم روز کی طرح واضح ہیں ۔ مرکزی جامع مسجد -N بلاک عارفوالا کی امامت اور خطابت کے فرائض بحسن و خوبی سر انجام دے رہے ہیں۔صاحب ورع اور تقویٰ ہونا اِن کی...

Comparative Effect Of Tetrapack Juices And Fresh Fruit Juices On Blood Glucose Level Among Healthy Individuals Comparative Effect of Juices on Blood Glucose Level

Uses of tetra pack juices enhances the risk of diabetes and obesity. People were unaware of impact of fresh fruit juices on health. Objective: To compare the effect of tetra pack juices and fresh fruit juices on blood glucoselevels among healthy individuals Methods: 10 participants were selected using an internationalstandard Glycemic Index (GI) test protocol. After getting their fasting blood sample theywereinstructed to consume all the juice served in a period of 5 min. Further blood samples were takenat different intervals of time that is 0, 30, 60, 90, 120 and 150 minutes after consumption. Participants wereremained sedentaryduring each session. Blood was obtained by finger-prick and tested by the glucometer. Results: The glycemic index of Apple juice Nestle has the glycemic index of 95.87, Orangejuice Nestle has the GI value 93.78, Apple juice fresh has the GI value of 92.17 and Orangejuice fresh has the glycemic index value of 99.07, respectively Conclusions: Detailed study of glycemic index of tetrapack juices (Nestle Apple and Orangejuice) and fresh fruit juices (Apple and Orange juice) showed similar impact on the blood glucose level of healthy individual. In the study, both types of juices were found to be equally hyperglycemic (GI 70+) causing a fast rise in blood-sugarlevels, hence should not be given to diabetic patients

Biomineralization of Cadusafos in Agricultural Soils As Influenced by Co-Occurring Antimicrobial Agents

Cadusafos is a soil-applied pesticide presented as effective against a broad spectrum of nematodes and soil insects on several important crops worldwide. With respect to its toxicity, it is classified as a pesticide of Class I (highly hazardous to human health) and is regarded as ‘dangerous to the environment’ by the regulatory departments. Its presence in the terrestrial environment may pose serious health risks. Triclosan (TCS) and triclocarban (TCC) are the antimicrobial agents that are routinely used in a large number of contemporary consumer and healthcare products. A major fraction of TCS and TCC is washed down the drain after use, and ends up in agricultural soils by the application of biosolids and untreated and partially-treated wastewater. Since TCS and TCC are biocides that are designed to kill microorganisms, their co-occurrence with cadusafos may prolong the persistence of cadusafos in terrestrial environment and cause serious health problems. While living in the soil, the co-existing pesticide and the antimicrobial agents remain in close interaction with the soil constituents, and thus, the soil physicochemical properties may be of vital importance for determining the persistence of the pesticide in the terrestrial environment. Hence, a series of experiments was conducted by using three agricultural soils of different physicochemical properties to study the effect of TCS and TCC on the microbial activity by spiking with biodegradation of 14 14 C-glucose, and on C-cadusafos in adapted (the soil which was previously exposed to cadusafos) and unadapted (the natural soil without previous exposure to cadusafos) agricultural soils. The soils were spiked with 14C-glucose or 14C-cadusafos @ 5 μg/g and TCS @ 30, 90, 270 μg/g or TCC @ 50, 150 and 450 μg/g soil in different experiments. The soils were maintained at 48 and 65% of maximum water holding capacity (MWHC) and were incubated at 28 ± 1 oC up to 28 or 77 days in different experiments. The results revealed a similarity in pattern of biomineralization of 14 C-cadusafos in all the three tested soils. The moisture levels had little effect on biomineralization of well as of 14 14 14 C-glucose as C-cadusafos while soils differed significantly in their potential to degrade C-cadusafos, most likely due to variation in their physicochemical properties. Kinetic analysis revealed that biomineralization of 14 C-cadusafos followed a first order kinetics during the incubation time of the study. Moreover, biomineralization of 14 C-cadusafos was negatively affected by the antimicrobial agents, TCS and TCC, but with different degree of efficacy. The effect of TCS was highly significant and concentration dependent whereas, a very weak and negligible effect of TCC was recorded in the three soils. The TCS application at its highest level (270 μg/g) reduced biomineralization of 14C-glucose in the absence or presence of D-glucose up to 53.6 and 50.1%, respectively. The same dose of TCS caused reduction in 14C-cadusafos biomineralization by 58.4 and 57.4% in the unadapted (fresh) and adapted (conditioned with 14C-cadusafos) soils, respectively. A strong negative correlation was identified between some physicochemical properties (pH and organic matter) and the effect of TCS on biomineralization of 14 C-glucose or 14 C- cadusafos. These findings indicate a strong role of antimicrobial agents, like TCS, in prolonging the persistence of xenobiotics such as cadusafos. This may imply that in addition to investigating the biomineralization of pesticides in soil environment, the continual loading of antimicrobial agents must also be given the due attention. The unrestricted use of the carriers of antimicrobial agents, like biosolids and untreated wastewater, should be avoided for keeping the terrestrial environment safe and healthy. In this connection, if necessary, the laws may also be enacted for the restricted