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New Matrix Development for the Matrix Assisted Laser Desorption/ Ionization Maldi for the Analysis of Biomolecules and Metabolites Profiling of Oral Cancer for Biomarker Search

Thesis Info

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Author

Shahid, Najia

Program

PhD

Institute

University of Karachi

City

Karachi

Province

Sindh

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/10261/1/Najia%20Shahid_Chem_2018_UoK_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726752523

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This dissertation comprises of seven chapters. First chapter summarizes the brief introduction of matrix assisted laser desorption/ ionization mass spectrometry (MALDI-MS), its theory, applications, and significance in small molecule analyses. The second chapter summarizes the brief introduction of the development of metabolomics in omics sciences with relevance to oncology. While third chapter includes the oral cancer pathogenesis, classification, and its associated biomarkers. Fourth chapter is based on the possible increment in MALDI-MS sensitivity by increasing the ion generation via use of dopants in MALDI-MS matrices. These dopants constitute commonly used dyes, along with conventional MALDI-MS matrices which can significantly increase the ion intensity of standard proteins. Moreover, such effect remains consistent when applied to real samples (RSC Adv. 2017, 7, 6598–6604). Fifth chapter describes the successful application of 31 compounds of acylhydrazide, and isatin Schiff bases as alternate UV-LDI matrices for the analyses of peptides (<2000 Da) with significantly low background signals and showed comparable results with the conventional matrix (AJAC, 2012, 3, 779-789). Chapter six focused on the identification of distinguished metabolites of oral cancer tissue samples in comparison with precancerous and control tissue samples using gas chromatography-mass spectrometry (GC-MS) and chemometric analyses. Metabolites obtained were identified through National Institute of Standards and Technology (NIST) mass spectral (Wiley registry) library. Mass Profiler Professional (MPP) software was used for the alignment and for all the statistical analyses. 31 compounds out of 735 found distinguishing among oral cancer, precancerous and control group samples using p-value ≤ 0.05. The sensitivity and specificity of built model was found to be 85.7% and 93.3%, respectively, using statistically significant metabolites (Scientific Reports, 2016, 6, 38985-38993). Last chapter of the dissertation based on the distinctive identification of metabolites of oral cancer, snuff dippers and healthy plasma samples using GC-MS and chemometric analyses. Metabolites obtained were identified through NIST mass spectral library. MPP software was used for the data pretreatment and statistical analyses. 29 compounds were found distinguishing among oral cancer, snuff dippers and healthy group samples using p-value ≤ 0.001 and fold change ≥3. PLSDA model was generated using statistically significant metabolites which gave an overall accuracy of 89.2% (Submitted in Head & Neck).
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مولانا سید محمد مرتضیٰ مظاہری

مولانا سید محمد مرتضیٰ مظاہری مرحوم
ندوۃ العلماء کے کتب خانہ شبلی کے ناظر مولانا سید محمد مرتضیٰ مظاہری بھی ۳؍ اور ۴؍ نومبر کی درمیانی شب میں لکھنؤ میں انتقال فرماگئے، وہ قریباً چالیس برس تک ندوہ کے کتب خانہ کی ترقی و تزئین کے لیے وقف رہے، لائبریری سائنس کی کسی مروجہ عصری سند کے بغیر انہوں نے جس طرح اس کتب خانہ کو ملک کا بہترین کتب خانہ بنایا وہ اپنی مثال آپ ہے، انہوں نے عرصہ تک ندوہ کے مدرسہ ثانویہ اور دارالاقامہ سلیمانیہ میں اتالیق و مربی کے فرائض بھی انجام دیے ندوہ اور مولانا سید ابوالحسن علی ندوی سے ان کو عشق تھا، ندوہ کے لیے انہوں نے کثرت سے سفر کئے، نہایت مرنجاں مرنج خوش اخلاق، ہنس مکھ اور خلیق و شفیق تھے دارالمصنفین سے بھی ان کو خاص تعلق تھا، سید صباح الدین عبدالرحمن مرحوم جب بابری مسجد مرتب کررہے تھے تو مولانا نے بڑی دلچسپی سے ان کو ضروری مخطوطات و کتابیں مہیا کیں جن کا اعتراف کتاب کے مقدمہ میں کیا گیا ہے، گزشتہ مہینہ جب دارالمصنفین میں رابطہ ادب اسلامی کے سمینار کا اعلان ہوا تو وہ اس میں شریک ہونے والے تھے، لیکن قضاو قدر نے اس سے پہلے ہی ان کو اپنے خالق حقیقی کے حضور میں بلالیا، اﷲ تعالیٰ پس ماندگان کو صبر جمیل اور مولانا مرحوم کو جنت الفردوس میں جگہ عطا فرمائے، آمین۔ (’’ع ۔ص‘‘، دسمبر ۱۹۹۵ء)

 

The Importance of Emerging Sciences & Technology in the Light of Quran & Prophetic Teachings

Science and technology is on top position contemporary now it is need of our masses to compare these formulas with holy Quran because Quran is miracle and implementable till the day of  Judge ment  lot of scientists have benefited by Quran and they also agree up on the excellence of Quran. Hence in this research we have to prove dominancy of Quran over on the science and technology the hints of new inventions and discoveries are available in Quran if we study the Quran deeply we will find in it complete agreement with modern science however it was revealed in that time people were not aware from scientific inventions and they have not know-how of it. Comparatively this time is well advanced period and lot of things invented while concept of these things was present in this sky book I have drawn intention of readers on these points. In this research I would discussed that importance of science and technology specially the sub subjects of the modern science math and statistics geology, astronomy, space sciences, Embryology, Genetics and computer technology. It should be remember that the exposition of this book is also two types scientifically and logically because Quran invites us towards intelligence and wisdom.   It is responsibility of scholars to interpret the Quran contemporary because this is not merely ancient book but it is for all times and not for Arabs but for guide line of whole humanity of entire universe. It is challenges itself that no any kind of error or mistake in it foes of Islam tried to find faults but they not succeeded to do so resulting they affirmed dignity of it over on all the books.

Some Cosmological Implications of Extended Scalar Tensor Theories

This thesis is devoted to explore some cosmological implications in gravity and generalizedgravity. Initially, we discuss the cosmological reconstruction of theory (where,and represent the Ricci scalar, scalar invariant and scalar field) corresponding to power law and de Sitter evolution in the framework of FRW universe model. We derive the energy conditions for this modified theory which seem to be more general and can be reduced to some known forms of these conditions in general relativity,andtheories. We also present the general constraints in terms of recent values of snap, jerk, deceleration and Hubble parameters. The energy bounds are analyzed for reconstructed as well as known models in this theory. Finally, the free parameters are analyzed comprehensively. First and second laws of black hole thermodynamics are examined at the apparent horizon of FRW spacetime in gravity. In this modified theory, Friedmann equations are formulated for any spatial curvature. These equations can be presented into the form of first law of thermodynamics for ̂̂ , wherêis an extra entropy term because of the non-equilibrium presentation of the equations and ̂ for the equilibrium presentation. The generalized second law of thermodynamics (GSLT) is expressed in an inclusive form where these results can be represented in GR,andgravities. Finally to check the validity of GSLT, we take some particular models and produce constraints of the parameters. Moreover, we examine static spherically symmetric wormhole solutions in generalizedgravity. To do this, we consider three different kinds of fluids: anisotropic, barotropic and isotropic. We explore differentmodels and inspect the energy conditions for all of those three fluids. It is found that under some models in this theory, it is possible to obtain wormhole solutions without requiring exotic matter. From our results, one can conclude that for all three cases of fluids stable and realistic wormhole solutions can be constructed.Further, we have consideredaction which is non-minimally coupled to the xi scalar field. In this context, we obtain the exact analytical solutions for inflationary era as well as find a graceful exit condition from inflation. We calculate the perturbed parameters, i.e., number of e-folds, slow-roll parameters, scalar and tensor power spectra, corresponding spectral indices and ultimately tensor to scalar ratio. It is showed that the power spectra lead to blue-tilt for this model. The trajectories of the perturbed parameters are plotted to compare the results with recent observations.Finally, we will discuss cosmological models using Bianchi type I for anisotropic fluid intheory of gravity which involves scalar potential. For this purpose, we consider power law assumptions of coupling function and scalar field along with the proportionality condition of expansion and shear scalars. We choose twomodels and obtain exact solutions of field equations in both cases. For these constructed models, the behavior of different physical quantities like EoS parameter, self-interacting potential as well as deceleration and skewness parameters is explored and illustrated graphically for the feasible ranges of free parameters. It is concluded that anisotropic fluid approaches to isotropy in later cosmic times for both models which is compatible with the observational data.