مولوی عبدالحمید ندوی
یہ سطور ابھی پریس نہیں گئی تھیں کہ مولوی عبدالحمید ندوی کے انتقال کی خبر ملی، وہ بارہ بنکی کے ایک گاؤں جیسکھ پور کے رہنے والے تھے، بڑے مخلص، حق پسند اور بہی خواہ خلق تھے، ۱۹۱۹ء میں ندوہ میں داخل ہوئے، تحریک خلافت شباب پر تھی، حمید صاحب بھی اس سے متاثر ہوئے، اس کی وجہ سے سادگی طبیعت ثانیہ بن گئی، مولانا عبدالرحمن نگرامی کی صحبت نے ان کے اندر ندوہ کی محبت اور خاموش خدمت کا جذبہ پیدا کیا، تعلیم سے فراغت کے بعد قیام زیادہ تر وطن ہی میں رہا، لیکن ندوہ برابر آتے جاتے رہتے تھے، دوستوں کے اصرار کئی برس تک بھٹکل میں تعلیمی خدمت انجام دی، ان کے مخلص شاگردوں نے اس کام کو آگے بڑھایا اور ایک بڑا تعلیمی مرکز قائم ہوگیا، عرصہ سے دل کے مریض تھے، رمضان میں ندوہ آئے، مولانا ابوالحسن علی سے ملنے رائے بریلی جانے کا ارادہ تھا، دفعتہ دل کا دورہ پڑا اور تھوڑی دیر میں جان جان آفریں کے سپرد کردی، اﷲ تعالیٰ ان کی مغفرت فرمائے اور ان کے صاحبزادہ مولوی عبدالرشید ندوی اور دوسرے متعلقین کو صبر جمیل عطا فرمائے۔ (عبد السلام قدوائی، ستمبر ۱۹۷۸ء)
The state of Bah┐walp┴r was founded in 1802 by Naw┐b Mohammad Bah┐wal Khan II. After the establishment of Pakistan the state opted to accede to the new, on 14 August 1947 October 1947. It was dominion of Pakistan, with effect from 7.1511 merged into the province of West Pakistan on 14 October Bah┐walp┴r has always been a seat of higher learning. Uch Shar┘f, a nearby ancient town, had one of the largest universities where scholars from all over the world used to come for studies. As a continuation of that tradition, J┐m‘a Abb┐siya was established in Bah┐walp┴r in the year 1925, following the academic pursuits of Jamia Al-Azhar, Egypt. The renowned scholars spread Islam by teaching Tafs┘r of Qura’n, Hadith, Fiqh, and History along with other contemporary subjects. The establishment of Jamia Abb┐sia and the arrival of religious scholars in Bah┐walp┴r bear witness that it is a scholarly and traditional state and it has been proved as a place of protection for educational, cultural and historical traditions.
Poultry industry is a dynamic sub-division of agriculture sector throughout the world as well as in Pakistan owing to rising global demand for this more affordable animal protein than red meat, due to exponential population growth and rapid urbanization in developing regions. However, the major problems encountered by the industry are infectious viral diseases; mainly infectious bursal disease (IBD), causing severe production and economic losses. Currently, live, attenuated or killed classical virus strains are used as vaccines to immunize birds but still outbreaks are reported even in vaccinated flocks probably owing to difference in antigenic structure between vaccine and field vvIBDV strains. Also, the current vaccine production systems including microbial, stable nuclear and transient plant viral technologies are not feasible in terms of cost and biosafety. So, as a preliminary step for development of clean quality and cost-effective recombinant subunit vaccine, a VP2 gene along with engineered choloroplast based Prrn promoter and PsbA terminator was synthesized commercially. Phylogenetic analysis and multiple sequence alignment with representative strains of different geographic origin confirmed the antigenic structure similarity of synthetic VP2 protein with those of circulating vvIBDV strains. Synthetic VP2 gene together with FLARE-S gene cassette was flanked by tobacco chloroplast flanking sequences through sequential cloning leading to development of final chloroplast transformation construct. Fully expanded 4-6 weeks old tobacco leaves were bombarded using a gene gun for chloroplast transformation. Initially, putative transplastomic lines regenerated on spectinomycin selection medium were selected by visual detection through gfp fluorescence as transplastomic sectors in a chimeric leaf tissue fluoresced green under UV light. These fluoresced sectors not only revealed heteroplasmic state of transgenic plants but also confirmed the successful integration of marker gene into tobacco chloroplasts. Putative transplastomic shoots were then subjected to additional rounds of selection and regeneration for developing homoplasmic clones. The genetic analysis of putative transplastomic plants through polymerase chain reaction confirmed the presence of VP2 as well as aada and gfp genes in the plastome. Integration of cassettes into inverted repeat region was confirmed using forward primer specific to aada gene and reverse primer specific to plastome. Further, development of an efficient regeneration system for soybean via somatic embryogenesis was attempted using 2 and 7 days old mature cotyledons from in-vitro germinated seedlings of a local ‘Faisal’ and an exotic ‘Jack’ cultivar with the intention to develop edible VP2 subunit vaccine as soybean is a chief constituent of poultry feed. It was observed that 7 days old mature cotyledonary explants of cultivar Jack were more responsive towards somatic embryogenesis than 2 days old ones and those of cultivar Faisal after callus induction on D20 medium directly. However, no regeneration was observed in either case indicating the need for further experimental investigation