Wheat is staple food of inhabitants of the Indian subcontinent and is grown all over the irrigated and rain fed areas of this region. This important crop is now under the threat of Karnal bunt disease caused by Tilletia indica; a fungus of basidiomycetes, that seriously affects the quality of the grains, by emitting fishy odour of tri-methylamine which makes the flour quality unacceptable for human consumption. Previously the disease was considered of minor importance and was prevalent in the areas of low temperature and high humidity only, but now it has got the status of emerging infectious quarantine disease in many parts of the world including Pakistan. Present studies were conducted to scrutinize the prevalence, infestation level of the infection and identification of genetic sources resistant to Tilletia indica pathogen. Studies were conducted in the wheat growing areas of the Southern Punjab that is under dry hot climate. Seven districts viz. Khanewal, Multan, Lodhran , Bahawalpur, Vehari, Bahawal Nagar and Rahim Yar Khan were selected to evaluate the disease as well as genotypes potential regarding the disease occurrence. During survey and monitoring of these localities forty nine hot spots of the karnal bunt disease were identified. Six high yielding commercial varieties were grown at these spots, out of which Punjnad-1 showed maximum capacity to resist the disease pressure followed by Inqilab-91. while Manthar -03 and Uqab- 2000.
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