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Home > Pharmacological Investigations on Some Indigenous Plants of Cholistan Desert

Pharmacological Investigations on Some Indigenous Plants of Cholistan Desert

Thesis Info

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Author

Naveed Aslam

Program

PhD

Institute

Bahauddin Zakariya University

City

Multan

Province

KPK

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Pharmacology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/13366/1/Naveed%20Aslam%20Pharmacy%202019%20BZU%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726879012

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Aims of study The present study was undertaken to validate traditional medicinal claims and to further explore pharmacological actions of four indigenous plants of Cholistan desert (Asphodelus tenuifolius, Corchorus depressus, Gisekia pharnaceoides and Salsola imbricata) in relation to gastrointestinal, respiratory and cardiovascular systems. Material and methods The dried and powdered plants materials were extracted by maceration using aqueous-ethanol as solvent to obtain crude extracts. The crude extracts, their ethyl acetate and aqueous fractions were tested for gastrointestinal, bronchodilator and cardiovascular activities; in vitro experiments were performed upon isolated tissue preparations of rabbit using standard tissue organ bath techniques and in vivo experiments were performed in mice and rats. Results The crude extract of Asphodelus tenuifolius (Cr.At) relaxed spontaneous, potassium (25 mM) and potassium (80 mM) mediated contractions in isolated jejunum preparations, while being equipotent in relaxing potassium (25 mM) and potassium (80 mM) mediated contractions, in manner similar to verapamil. Pretreatment of jejunum preparations with Cr.At or verapamil shifted calcium concentration response curves (CRCs) rightward with suppression of maximum effect. Aqueous fraction of Cr.At exhibited contractile effect upon spontaneous contractions in jejunum preparations. Oral administration of Cr.At to mice caused significant increase of charcoal meal intestinal transit at dose of 100 mg per kg; enhanced wet and total feces counts at doses of 50 and 100 mg per kg; but it significantly decreased charcoal meal intestinal transit at 500 mg per kg dose. Cr.At (300, 500 and 700 mg per kg; orally) reduced castor oil induced diarrhea in mice. In rabbit isolated tracheal preparations, Cr.At was more potent against potassium (80 mM) than carbachol (1 µM) mediated contractions and shifted carbachol CRCs rightward in non-parallel fashion. In rabbit isolated aortic preparations (endothelium denuded), Cr.At was more potent against potassium (80 mM) than phenylephrine (1 µM) mediated contractions. In isolated rabbit paired atria, Cr.At caused overall negative inotopic and chronotropic effects. Intravenous administration of Cr.At (3-30 mg per kg) to anesthetized normotensive rats produced hypotensive effect, which remained unchanged on pre-treating the animals with atropine. Spasmolytic constituents were found partitioned in ethyl acetate fraction of Cr.At, whereas aqueous fraction was found to contain spasmogenic activity. The crude extract of Corchorus depressus (Cr.Cd) relaxed spontaneous, potassium (25 mM), potassium (80 mM) and carbachol (1 µM) mediated contractions in rabbit isolated jejunum preparations; being most potent in relaxing carbachol (1 µM) mediated contractions, and equally potent in relaxing potassium (25 mM) and potassium (80 mM) mediated contractions, similar to that of dicyclomine. It shifted calcium CRCs upon jejunum preparations rightward in non-parallel manner, similar to that of dicyclomine. Aqueous fraction of Cr.Cd exhibited atropine sensitive contractile effect on spontaneous contractions of rabbit jejunum preparations. Oral administration of Cr.Cd to mice (50 and 100 mg per kg) caused significant increase in charcoal meal intestinal travel, enhanced formation of wet and total stool; but at 300 and 500 mg per kg doses, it significantly decreased charcoal meal intestinal travel. It also reduced castor oil induced diarrhea in mice at oral doses of 500 and 700 mg per kg. In isolated tracheal preparations, Cr.Cd was more potent in relaxing carbachol (1 µM) than potassium (80 mM) mediated contractions and shifted carbachol CRCs rightward, similar to that of dicyclomine. In isolated rabbit aorta, Cr.Cd relaxed potassium (80 mM) mediated contractions. In isolated rabbit paired atria, Cr.Cd was found to possess atropine sensitive cardio-depressant activity. Intravenous administration of Cr.Cd (1-30 mg per kg) to anesthetized normotensive rats produced hypotensive effect, which was reversed upon pre-treating animals with atropine. Gisekia pharnaceoides extract (Cr.Gp) and its ethyl acetate fraction caused preferential inhibition of potassium (25 mM) than potassium (80 mM) mediated contractions in rabbit isolated jejunum, tracheal and aortic preparations; which was attenuated in presence of glibenclamide (3 µM), in a manner similar to that of cromakalim. The aqueous fraction of Cr.Gp produced contractions on baseline of rabbit jejunum, tracheal and aortic preparations; which were attenuated subsequent to pre-incubation of tissues with caffeine (10 mM). Cr.Gp was found to possess glibenclamide sensitive cardio-depressant effects upon spontaneously beating rabbit paired atria. Oral administration of Cr.Gp to mice (50 and 100 mg per kg) resulted significant inhibition of castor oil induced diarrhea as well as significant decrease in charcoal meal intestinal travel; but at higher doses (300 and 500 mg per kg), it significantly increased charcoal meal intestinal travel and increased formation of diarrheal feces in normal mice. Intravenous administration of Cr.Gp (1-30 mg per kg) to anesthetized normotensive rats produced hypotensive effect. The crude extract of Salsola imbricata (Cr.Si) relaxed spontaneous, potassium (25 mM), potassium (80 mM) and carbachol (1 µM) mediated contractions in smooth muscle preparations of rabbit isolated jejunum. Pretreatment of tissue preparations with Cr.Si shifted calcium CRCs toward right with suppression of maximum effect, in a manner similar to verapamil. Pretreatment of tissue with propranolol (1 µM) partially antagonized the relaxant activity of the extract upon carbachol mediated contractions, similar to that of isoprenaline. Oral administration of Cr.Si (100, 300 and 500 mg per kg) in mice caused inhibition of charcoal meal intestinal travel and prevented castor oil induced diarrhea. In smooth muscle preparations of rabbit isolated trachea, Cr.Si relaxed potassium (80 mM) and carbachol (1 µM) mediated sustained contractions equipotently; shifted carbachol CRCs rightward in non-parallel fashion; and propranolol (1 µM) decreased its relaxant effect upon carbachol (1 µM) mediated contractions. In isolated aortic preparations, the Cr.Si caused doxazosin sensitive contractions upon baseline tension and relaxed potassium (80 mM) mediated contractions. Cr.Si demonstrated cardiotonic activity upon spontaneously beating paired atria, which was reversed in presence of propranolol (1 µM). Intravenous administration of Cr.Si (1-30 mg per kg) to normotensive anesthetized rats produced dose dependent hypertensive effect, which was blocked in co-presence of propranolol and doxazosin. Calcium channel blocking constituents were found to be concentrated in ethyl acetate fraction, whereas aqueous fraction of Cr.Si was found responsible for aortic contractions. The crude extracts, when administered orally to rats, increased urine and urinary electrolytes (Na+, K+ and Cl-) excretion to different extent. Conclusion The study concludes that Cr.At, Cr.Cd and Cr.Gp possess gut modulatory, bronchorelaxant, hypotensive and diuretic activities; whereas Cr.Si was found to exhibit gut relaxant, bronchorelaxant, hypertensive and diuretic activities. Possible mechanisms responsible for the observed pharmacological activities of the extracts include, but not limited to, calcium channel blocking mechanism in Asphodelus tenuifolius; calcium channel blocking, muscarinic and antimuscarinic mechanisms in Corchorus depressus; ATP dependent potassium channel opening and calcium channel blocking mechanisms in Gisekia pharnaceoides; and calcium channel blocking and adrenergic receptors agonistic mechanisms in Salsola imbricata. The study validated some of the ethnic medicinal claims of the plants along with mechanistic background.
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میں ول ول تکناں مرشد نوں

میں ول ول تکنا مرشد نوں
مار گیا سوہنیا ایہہ مینوں تیرا پیار وے
دل کرے میں ویکھاں تینوں بار بار وے

تیریاں اداواں مینوں بڑا ای ستاندیاں
جدوں یاد آوے تیری بڑا ای رواندیاں
اکھیاں وی ہر ویلے مینہ برساندیاں
جے توں آویں فیر آوے دل نوں قرار وے
مار گیا سوہنیا اے مینوں تیرا پیار وے

جدوں دا میں ویکھا تینوں ہوش میری بھل گئی
چھڈ کاروبار میں تاں گلیاں چ رُل گئی
سارے بھار ہولے ہوئے ککھاں نال تُل گئی
مینوں چنگی لگی تیرے کجلے دی دھار وے
مار گیا سوہنیا اے مینوں تیرا پیار وے

مکھ تیرا ایویں جیویں چن اسمانی اے
ویکھے جو وی ہک واری ہووے اوہ دیوانی اے
اجڑیاں دلاں اُتے ہووے مہربانی اے
ہک واری دے جا مینوں اپنا دیدار وے
مار گیا سوہنیا اے مینوں تیرا پیار وے

تینوں جد تکیا تے میں تیری ہوئی وے
جگ وچ تیرے جیہا دسدا نہ کوئی وے
مینوں سد لے توں میرا یار ’’سنگوئی‘‘ وے
تیرا اوتھے رج رج کراں گی دیدار وے
مار گیا سوہنیا اے مینوں تیرا پیار وے

قادریؔ سائیں ہن نہ گھبرا توں
ٹلے جا کے مندراں وی گل وچ پا توں
جوگی بن در در بین بجا توں
خلق کرے گی فیر تیرے نال پیار وے
مار گیا سوہنیا اے مینوں تیرا پیار وے

Impact of Covid-19 on Interest Rates

The global economy is experiencing a crisis due to the Covid-19 pandemic, the stock market index has collapsed. The rupiah exchange rate against the USA dollar weakened this was due to the large number of foreign investors leaving the Indonesian financial market, the stock market plummeted. The banking sector can carry out an economic stimulus given restructuring authority for all credit or financing without requiring restrictions on the credit ceiling or type of debtor, especially debtors for MSMEs and informal workers. The economic stimulus that needs to be maximized is prundential monetary and macro policies through lowering interest rates and maintaining stability in the rupiah exchange rate. Budget relocation is also enforced to maintain the availability of basic foodstuffs for the community, which has increased due to panic buying or market panic. Also providing assistance to increase people's purchasing power.

Human Rights in Pakistan With Special Focus Bonded Labour

This study explores the issue of human rights of bonded labor and women in Pakistan. Bonded labor and women are very weak segments of our policy focus and empirical research. The study provides new insights into the thematic exploration in a qualitative study. The data was accessed through interviews, witnesses and oral opinions from the respondents. Meetings, observations and informal methods of data collection were also used. This has focused to investigate how, when and where human rights of bonded/fortified workers have been violated. The study provides a theoretical perspective on the roots and history of human rights. Human Rights can be defined as the most inalienable rights of the people ensured by the constitution and safeguarded by the judiciary and criminal justice system. Human rights in Islam are derived from the Will of God. God has created humans equally and there is no discrimination on the basis of caste, creed and color. However, before Allah the righteous are highly valued. No political authority but Allah alone has sole power of governing the rights of global humanity. The Human Rights of laboring class is highly challenging theme especially in context of Pakistan. Pakistan belongs to kind of a country where millions of people are undergoing hard labor. There are substantial pockets of laboring in all provinces of Pakistan. The earliest theorists like Karl Marx and Mex Weber meticulously focused on laboring perspective, labor economy, capitalistic injustices and low wages of laboring population. Capitalists have created an unequal structure for the poorest classes enabling powerful class to control their fate. Social and economic structures thus created will never secure and protect the laboring population on any part of the globe. World would be never stable without balancing the inequitable resources. Slavery and labor victimization are continued all over the world in various forms and manifestations. Country like Pakistan is no exception to the bitter scenario. This study is conducted in provinces of Sindh, Punjab and Baluchistan on the laboring in the context of their human rights status. Interviews, witness opinions, case-profiles and secondary information were utilized in the study. As research design the study included data collected from twenty one (21) districts along with information related to twenty six (26) districts of Baluchistan. The study focused the eleven (11) localities from Sindh province and seven (7) regions of Balouchistan. Further, the field study included its activity to talking to respondents (52 percent) of area of Sindh and twenty seven (27) belonging to Baluchistan. Women have been symbolized as commodity for male members in tribal structure. Women are considered Zar (wealth) which is possessed by men. There are terms like Zar (wealth), Zamin (land) and Zan (woman) which actually portray and reflect how we treat our women. Women face all sorts of sacrifices when revenges/enmities are settled. (Mehdi, 2000) It is very complex to investigate the issue of Bonded labor in Pakistan due to several constraints rooted in culture, society and political milieu. It is very difficult to know and address the slavery and servitude in Pakistan. Asian Development Bank has indicated a significant volume of destitution and slavery occurring in Sindh and Baluchistan. People are bonded by strongers and kept in captivation. Labor violations in Sindh are deep-rooted in land holdings and nature of distribution of lands. There is very high number of landless farmers in Sindh. Punjab has proprietorship on seven sections of land and Sindh has possession on twenty eight sections of land. International Labor Organization (ILO) depicts 1.8 million people who are involved in fortified work in Pakistan while majority of them are landless peasants found in Sindh. Today, Sindh has the most elevated rate of landlessness in Pakistan. More than 40 percent of the area in Sindh is tenanted out by enormous proprietors. There are about 1.2 million who are fortified in work along with their women and children. Sindh Tenancy Act has failed to protect the purview- fortified workers. The Bonded Labor Abolition Act has also failed to abolish Bonded Labor in Pakistan. Female babies born from age one to four are highly susceptible to death, disease and malnutrition. Women are highly victimized physically in South East Asia besides hindering them in development role. Women are denied access to human rights in rural areas in the study region and its purview. Children are engaged/forced/involved. Fate of women are settled by tribal jirga laws which have no statutory status in Pakistani laws but are popularly practiced in backward areas of Pakistan. Killing and other serious issues related to women are settled by family or tribal chief reviewed by tribesmen. Human societies still face grave issues of poor-child rearing, poor nutrition, forced labour, lack of sanitation and illiteracy. Girls face more severe social challenges in poor families and regions. The study concluded that seventy (70) percent rural women and eighty (80) percent urban women are thought inferior to men comparatively. Popularly; women are born for social bondage and marriage. Female babies born from age one to four are highly susceptible to death, disease and mal-nutrition. Women are highly victimized physically in South East Asia besides hindering them in development role. Women are denied access to human rights in rural areas in the study region and its purview. Children are engaged/forced/involved the activities in which children are employed are hazardous, including rag picking, leather tanning, coal mining, deep-sea fishing, brick making, carpet weaving, and manufacturing surgical instruments and glass bangles. Children working in the glass bangle sector are exposed to high temperatures, unstable material, fumes, and sharp objects. Children working in the tannery sector are exposed to toxic chemicals, and those working in the brick sector lift heavy loads. Children working in carpet-weaving suffer eye and lung diseases due to unsafe working conditions. Child miners, child domestics, and other working children who are far from their families are particularly vulnerable to sexual abuse. ‣ Bonded child labor reportedly exists in the coal, brick, and carpet industries. Some children working in mining, domestic service, and agriculture are from families who are bonded or indebted to their employers. Commercial sexual exploitation of children continues to be a problem. Children are trafficked within Pakistan for the purposes of sexual exploitation and bonded labor. Girls are trafficked internationally for forced labor. ‣There are reports of children being kidnapped, maimed, and forced to work as beggars. There also reports of children under the minimum voluntary recruitment age of 17 years being involved in armed conflict. Children below age of 14 are employed in hard labour and industrial work. This is pure violation of their human rights. Children are exposed to all types of harzards in Pakistan. Bonded labour is gross violation of law leading to maximum five years of prison and fine. International Law on children also prohibits trafficking of children and amounts to criminal implications for the offender. Prostitution of all kinds is globally prohibited by world laws and penal codes of all respective countries. There are no generally acknowledged figures for the quantity of reinforced youngster workers in India. Once more, Government''s dedication to general instruction and destitution annihilation projects have brought about noteworthy diminishing in number of reinforced works. In the customary commercial ventures of astounding hand-woven fabrics and painstaking work, expanded mindfulness by universal purchasers and stringent weighs put set up by multinational companies on their suppliers has brought about suppliers and producers to supplant reinforced tyke work by rather offering instructive offices to offspring of their representatives and laborers. Global Tourists to places like Rajasthan additionally have influence and have at commonly reported occurrences of tyke work to powers who quickly act to check any youngster work. Conversely, of 20 million fortified workers in Pakistan 7.5 million are youngsters." xml:lang="en_US