Search or add a thesis

Advanced Search (Beta)
Home > Pharmacological Prospecting of Rhus Punjabensis Stewart, Bioassay Guided Isolation and Characterization of Lead Compounds

Pharmacological Prospecting of Rhus Punjabensis Stewart, Bioassay Guided Isolation and Characterization of Lead Compounds

Thesis Info

Access Option

External Link

Author

Tabassum, Saira

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Biotechnology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/13651/1/SAIRA%20TABASSUM%20PH.D%20COMPLETE%20THESIS%2025.5.18.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726879828

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


The endorsement of ethno-botanical data from the under explored folk plant remedies represent an illimitable reservoir of novel compounds for drug discovery. Nature has always been the most imperative source of novel drugs against a number of unremitting and challenging health anomalies since antiquity. The present work was aimed to discover natural compounds from untapped natural source. After an extensive survey and information gathered by local vicinity, Rhus punjabensis Stewart (Anacardiaceae) plant was selected from Pakistan for investigation. For initial screening, a total of 22 extracts from leaf and stem parts of R. punjabensis were prepared by using total ‗11‘ different extraction solvent of escalating polarity. All these extracts were subjected to standard phytochemical and in-vitro biological evaluation. The phytochemical assays included standard chromogenic assays for the determination of total phenolic (TP) and total flavonoid contents (FC) and specific polyphenol quantification by RP-HPLC. Multimode antioxidant, antimicrobial, cytotoxic, antileishmanial, nuclear factor kappa-B (NF-κB) and mitochondrial transmembrane potential (MTP) detection assays were employed to assess the in-vitro biological prospective of the subject plants. All-inclusive, the results of initial screening were promising and led to the selection of R. punjabensis leaves as the most bioactive plant material for further appraisal. Briefly, maximum total phenolic and flavonoid contents were determined in the polar solvents extracts whereas, substantial amounts of gallic acid, rutin, apigenin and catechin were quantified in the moderately polar solvents extracts. A proficient antioxidant stature in terms of DPPH free radical scavenging (FRSA), total antioxidant capacity (TAC) and reducing power potential (RP) was also manifested by the moderately polar extracts of R. punjabensis leaves. A noteworthy antibacterial activity (MIC 1.11 µg/mL) against S. typhimurium. Sulphorhodamine B (SRB) cytotoxicity against human cancer cell lines (DU-145, IC50 value of 11.11 µg/mL), (HL-60, IC50 value of 10.82 µg/mL), and NF-κB based chemo-preventive proficiency was demonstrated by the ethyl acetate leaf extract.. All the extracts/fractions were tested for their anticancer and cancer chemopreventive activity by employing different independent assays. Overall the results of the initial screening were very encouraging and led to the selection of the most potent plant material for further investigation. Keeping in view the results of aforementioned assays, ethyl acetate was selected as the extraction solvent to proceed for preparative scale extraction. A total of 6 fractions were prepared from the crude ethyl acetate extract of leaves (863 g) by employing solid phase extraction as the fractionation technique. Bioassay directed isolation from active fractions using column chromatography led to the isolation of 5 bioactive compounds, n-hexane (n-hex), chloroform+n-hexane (n-hex+CHCl3) and ethyl acetate (EtOAc) fractions showed significant activities against tested bioassays. Structural elucidation of these compounds was conceded by infrared spectroscopy (IR), nuclear magnetic resonance spectroscopy (1D and 2D NMR) and mass spectrometry (MS). Compound 1 (RPPEC1) showed substantial antibacterial potential against S. typhimurium and B. bronchiseptica (MIC: 11.11 µg/mL) as well as antileishmanial activity (IC50 11.6 µg/mL). Compound 2 (PEEA-C2) demonstrated noteworthy cytotoxic activities against DU-145 and HL-60 human cancer cell lines with an IC50 value of 11.2 and 15.2 μg/mL, respectively. It also exhibited chemopreventive prospective in terms of inhibition of NF-κB (IC50 value of 19.4 μg/mL) and MTP (IC50 value of 28.6 μg/mL). Compound 3 (PEEA-C3) manifested noteworthy cytotoxicity against DU-145 cell line (IC50 value of 21.72 μg/mL). Compound 5 (RPEA-C5) demonstrated significant antibacterial spectrum against all the tested gram positive and gram negative strains while compound 4 manifested significant antibacterial activity against S. typhimurium (MIC 11.1 μg/mL) and cytotoxicity against DU-145 cell line (IC50 value of 29.5 μg/mL). The present study have been reported for the first time from R. punjabensis total five bioactive compounds were isolated, among them compound 4 (RPEA-C4) is a new compound.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

مولانا سید محمد میاں

مولانا سید محمد میاں
افسوس ہے ہماری انجمن علم وعمل کی ایک اورشمع روشن بجھ گئی، یعنی مولانا سید محمد میاں نے مختصر علالت کے بعد۷۴برس کی عمر میں۲۲/اکتوبر کوعین مغرب کے وقت ارون ہسپتال میں داعی اجل کو لبیک کہااور راہی ملک بقا ہوگئے۔اناﷲ واناالیہ راجعون۔مولانا گوناگوں علمی وعملی کمالات کے جو ایک شخص میں شاذونادر ہی جمع ہوتے ہیں جامع تھے، ایک طرف وہ بلند پایہ عالم،فقیہ ومحدث تھے دوسری طرف جنگ حریت وآزادی کے نہایت بہادراور بے خوف سپاہی، ایک طرف مورخ ومحقق اورکثیر التصانیف مصنف اوردوسری جانب اعلیٰ دفتری تنظیمی صلاحیتوں کے مالک،ایک طرف عابد شب زندہ دار اوردوسری طرف نہایت متواضع اورخلیق وملنسار،بے لوث وبے غرض،نام ونمود سے دور،شہرت ووجاہت طلبی سے نفور،نرم دم گفتار اورگرم بوقت ِپیکار۔ مرحوم دیوبند کے سادات رضوی سے تعلق رکھتے تھے اس لیے دیوبند میں پیدا ہوئے اوروہیں ازاوّل تاآخر تعلیم حاصل کی۔ فراغت کے بعد بعض مقامات پرمدرس رہے مگر پھر جمعیت علماء سے وابستہ ہوئے تو اسی کے ہوکر رہ گئے۔ وہ مولانا حفظ الرحمن سیوہاریؒ کے دست راست تھے، اس سلسلے میں کئی مرتبہ جیل بھی گئے، باتیں کم کرتے تھے اورکام زیادہ، نہایت سمجھ بوجھ اورہوش وگوش کے انسان تھے اورنہایت چست اور مستعد۔ حقیقت یہ ہے کے دفتری نظم ونسق کا بھرم ان کے دم سے قائم تھا۔اگرچہ ایک عرصہ سے درس وتدریس کاباقاعدہ سلسلہ نہیں رہاتھا لیکن مطالعۂ کتب اور تصنیف وتالیف کاذوق فطری تھا اس بناپر جمعیت علماء کی ہنگامہ خیز اور شبانہ روز مصروفیات کے باوجود وہ پابندی سے اس میں بھی لگے رہے ،چنانچہ اسی زمانہ میں دوکتابیں ’علماء ہند کاشاندار ماضی‘(تین جلدوں میں)اور’ علماء حق‘(۲ جلدوں میں) ان کے قلم سے نکلیں اورشائع ہوتے ہی ارباب علم وذوق کے حلقوں میں مقبول و مشتہر ہوگئیں، مشرق ومغرب میں ان سے استفادہ کیا گیا اور ان دونوں...

تفسیر روح المعانی کی روشنی میں حضرت عیسی (علیہ السلام) کی رفع سماوی کا تحقیقی جائزہ

Jesus of Nazareth is the central figure of the Christian religion, a savior believed to be both God incarnate and a human being. He is also known as Jesus Christ, the term “Christ” meaning anointed or chosen once. Most of the details of his life are unclear, and much of what is known about his life comes from the four Gospels of the Bible. The Gospels tell the story of Jesus’s auspicious birth in a stable in Bethlehem, and then of his life as an adult, a teacher with miraculous powers who foretold his own death to his closest followers, called apostles. Jesus, betrayed by the apostle Judas, was crucified by the Romans, and his resurrection three days after his death was taken as proof of his divinity. The date of Jesus’s birth to Mary is celebrated each December 25th as Christmas Day. The occasion was used as the base year for the modern Christian calendar, though researchers now believe that earlier estimates were inexact and that Jesus was actually born between 4 B.C. And 7 B.C. The date of the crucifixion is now marked as Good Friday, and the resurrection celebrated as Easter.

Improvement of Potato Solanum Tuberosum L. for Abiotic Stress Tolerance Through Genetic Engineering

Input applications of agricultural and industrial activities have increased salt levels in our soils. In the modern era of agricultural production of crops, shortage of water resources makes them inaccessible for growing crops. Plant species have different mechanisms that deal with the salt tolerance; but the capability to sustain low cytosolic Na+ is supposed to be one of the vital factors of plant salt tolerance. Removal of Na+ from the cytoplasm of the cells and/or the maintenance of the low cytosolic Na+ concentrations is carried out either by pumping Na+ out of cells (plasma membrane antiporter) or into the vacuoles (vacuolar antiporter) under high salinity conditions. This process is brought about by the operation of plasma membrane-bound H+-pumps responsible for energizing Na+/H+ antiporters. In addition to this, engineering of the regulatory machinery involving transcription factors has emerged as a new tool now for controlling the expression of many stress-responsive genes. Development and use of transgenic plants with enhanced capability of salt tolerance by over-expression of genes may help to meet the future challenges of abiotic stresses. The effect of plant growth regulators in different combinations on in vitro regeneration of currently grown potato cultivars (cvs). ‘Kuroda’, ‘Cardinal’ and ‘Desiree’ were determined. Overall, the callus production and in vitro regeneration efficiency was maximum in Cardinal and Kuroda. Media combination and cultivars having potential for good callus induction and regeneration were selected for transformation. The novel synthetic HSR1 gene was cloned under double CaMV35S promoter in the pGreen0029 plant expression vector. HSR1 and AVP1 genes were transformed in potato through Agrobacterium-mediated transformation method. Putative transgenic calli and regenerated shoots were obtained in the presence of kanamycin (50 mg/ml) as plant tissue selection agent. Varying transformation efficiencies (30 and 25 %) were observed in different batches for Kuroda and Cardinal, respectively. A total of 57 transgenic plants were obtained from independent events and were successfully established in pots containing sterilized sand. Transgenic plants were confirmed by PCR and Southern hybridization. Variable numbers (1-4) of integration sites for the transgenes were observed in the genomic DNA of transgenic potato plants when AVP1/HSR1 specific probes were used for Southern analysis. In order to check abiotic stress tolerance potential, transgenic plants were subjected to in vitro screening in response to different levels of stress inducing agents like NaCl and PEG (6000). Agronomic parameters (shoot length, root length, leaf area index, shoot fresh weight, shoot dry weight, root fresh weight, root dry weight and relative water contents) were recorded that shows the transgenic potato lines performed better under stress conditions compared to the control plants. Transgenic potato plants containing AVP1 and synthetic HSR1 genes were analyzed for salt stress tolerance. Significantly (P ≤ 0.05) higher photosynthetic rates, stomatal, sub-stomatal conductance and transpiration rate were observed in transgenic plants harboring HSR1 and AVP1 genes compared to control plants. Higher Membrane Stability Index was noted in transgenic plants than non-transformed plants. Transgenic plants showed higher accumulation of Na+, K+ and a higher Na+/K+ ratio than non-transformed plants. Salt analyses showed high accumulation of total free amino acids, proline contents, and total soluble sugars indicating salt tolerance manifested by transgenic AVP1 and HSR1 plants. To avoid the toxic level of ROS and protect the cells from oxidative injury, accumulation of complex antioxidant enzymes such as superoxide dismutase (SOD) (EC 1.15.1.1), peroxidase (POD) (EC 1.11.1.7), ascorbic peroxidase (APX) (EC 1.11.1.11) and catalase (CAT) (EC 1.11.1.6) were determined in stressed leaves which showed a significantly higher accumulation in transgenic potato plants compared to controls. Tunnel experiment was performed for yield components to check the potential of transgenic plants under various levels (100, 75 and 50%) of drought stress. Significantly (P ≤ 0.05) higher biomass, number of tubers/plant and weight of tubers/plant was observed at 75 and 50% of drought stress. This study provides an efficient protocol for regeneration efficiency of potato cultivar Kuroda, Cardinal and Desiree using internodal explants. The results suggest that transgenic plants expressing higher levels of AVP1 and HSR1 transcripts in potato are able to withstand salt and drought stress regimes.