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Home > Phylogenetic Relationships in Apocynaceae Based on Both Nuclear and Plastid Molecular Datasets

Phylogenetic Relationships in Apocynaceae Based on Both Nuclear and Plastid Molecular Datasets

Thesis Info

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Author

Nazar, Nazia

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2012

Thesis Completion Status

Completed

Subject

Botany

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1595

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726893722

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The phylogenetic relationships within Apocynaceae were investigated in the present study. In addition trnL-F intron-spacer region and atpB promoter, a part of PHYA exon, a low-copy nuclear gene were sequenced from Apocynaceae. Different taxa of the family were collected from Pakistan and different regions of the world, representing major groups of the family. Separate phylogenetic trees were constructed using trnL-F and PHYA sequences and then combined datasets were used for simultaneous analysis. In the separate trnL-F analyses (comprised of 178 taxa with updated nomenclature) both parsimony and Bayesian, yield a number of stable clades, but placement of tribes (Vinceae, Tabernaemontaneae, Hunterieae and Melodineae) in Rauvolfioideae is uncertain and lack high level of support. A clade comprising Ceropegieae and Marsdenieae receives good support confirming the monophyly of both tribes. The grouping of taxa in Asclepiadeae is not satisfactory to define a subtribal classification. Malagasy Cynanchum group forms a separate clade in both analyses while the monophyly of New World Cynanchineae is not supported here. In the combined phylogenetic analyses, 112 taxa were included representing most major caldes in Apocynaceae. The study confirms that Periplocoideae are nested within Apocynoideae. The APSA clade (Apocynoideae, Periplocoideae, Secamonoideae and Asclepiadoideae) is strongly supported here, but the crown clade of Apocynaceae (comprised of subfamilies Asclepiadoideae, Secamonoideae, Periplocoideae and Echiteae, Mesechiteae, Odontadenieae and Apocyneae of Apocynoideae) has only moderate support. The present study places Periplocoideae as part of the sister group to the rest of the crown clade and the tribe Baisseeae emerges as sister group of Secamonoideae-Asclepiadoideae clade. Old World Cynanchinae form a well-supported clade with the New World MOG (Metastelmatinae, Oxypetalinae and Gonolobinae) tribes rather than with the largely Old World Asclepiadinae and Tylophorinae, as suggested by earlier studies. By addition of atpB promoter sequences of Rauvolfioideae’s taxa in combined dataset (trnL-F and PHYA), the inter-generic resolution was not improved in the subfamily. In the present study, resolution among most groups (such as inter-tribal relationships of Asclepiadoideae) is improved in combined analyses as compared to previous phylogenies, based on only plastid regions. However, there is a need to sequence vmore nuclear loci like PHYA from greater number of taxa to further improve relationships in the family.
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خیر ہی خیر سر بہ سر ہونا

خیر ہی خیر سر بہ سر ہونا
کتنا مشکل ہے بے ضرر ہونا

پوچھ اُن سے جو لوگ بے گھر ہیں
کیسا ہوتا ہے اپنا گھر ہونا

زخم نے جا لیا رگِ جاں کو
کیا ہوا تیرا چارہ گر ہونا

نہ سنے نالے آسماں نے مرے
ہائے نالوں کا بے اثر ہونا

کتنی آساں ہے خوب تر کی طلب
کتنا مشکل ہے خوب تر ہونا

دے گیا عمر بھر کے پچھتاوے
سب دعائوں کا بے ثمر ہونا

پا لیا رازِ زندگی تائبؔ
آ گیا کام در بہ در ہونا

ANALISIS NILAI-NILAI TOLERANSI DALAM BUKU “SENANG BELAJAR AGAMA ISLAM DAN BUDI PEKERTI” DI KELAS IV DAN V SD DI KAB. KARANGANYAR “SENANG BELAJAR AGAMA ISLAM DAN BUDI PEKERTI” DI KELAS IV DAN V SD DI KAB. KARANGANYAR

Oleh Rizky Martha Oktavia Prodi Pendidikan Agama Islam, Pascasarjana, IAIN Surakarta rizkymarthaoktavia@gmail. Com   Abstrak Kesuksesan pendidikan toleransi melalui pendidikan agama Islam dapat ditopang oleh komponen bahan ajar, misalnya buku “Senang Belajar Agama Islam dan Budi Pekerti” bagi Siswa Kelas IV dan V SD di Kab. Karanganyar. Permasalahannya, bagaimana analisis nilai-nilai toleransi dalam buku tersebut? Tujuan penelitian, yaitu menganalisis nilai-nilai toleransi dalam buku “Senang Belajar Agama Islam dan Budi Pekerti” bagi Siswa Kelas IV dan V SD di Kab. Karanganyar. Dengan pendekatan kualitatif dan jenis penelitian pustaka, data penelitian berupa nilai-nilai toleransi diperoleh dari sumber data buku dikumpulkan dengan studi dokumentasi. Keabsahannya diperiksa dengan teknik (1) triangulasi, (2) pengecekan anggota, (3) meningkatkan ketekunan, dan (4) kecukupan bahan. Berdasarkan hasil analisis isi ditemukan nilai-nilai toleransi, yakni 31 nilai toleransi, misalnya (1) tidak menertawakan cara beribadah umat beragama lain, (2) tidak membicarakan kekurangan agama umat beragama lain, (3) tidak mencela aturan beribadah umat beragama lain. Saran kepada penulis buku Pendidikan Agama Islam (PAI) SD, yakni perlu penyebaran nilai toleransi pada setiap bab; guru PAI dan siswa kelas IV dan V SD perlu membaca, bahkan menelusuri nilai toleransi agar dapat diajarkan berkelanjutan; siswa kelas IV dan V SD perlu membaca dan menerapkan nilai toleransi dalam buku dengan berkonsultasi pada orang tua dan guru yang membinanya; kepala SD dapat menjadikan sebaran nilai-nilai toleransi sebagai panduan untuk memilih, menentukan, dan merancang kegiataan sosial kemasyarakatan di sekolah untuk melatih kepekaan siswa menjadi generasi yang toleran. Kata-kata Kunci: nilai toleransi, budi pekerti, pendidikan                               agama Islam

Need of Guidance at Secondary School Level

Laboratory tests were conducted to determine the acute toxicity of Al, As, Ba, Cr and HMM, in terms of 96-hr LC50 and lethal concentrations, to three age groups viz. 60-, 120- and 240-day of Catla catla, Labeo rohita and Cirrhina mrigala at constant water temperature (30oC), pH (7.50) and total hardness (300 mgL-1). The growth performance, condition factor, feed intake and feed conversion efficiency of 240-day three fish species viz. Catla catla, Labeo rohita and Cirrhina mrigala under chronic (1/3 of LC50)exposures of Al, As, Ba, Cr and their mixture for 90 days were also determined. The fish were fed with feed (34% DP and 3.00 Kcalg-1 DE) to satiation daily. At termination of each trial, the fish were dissected and their organs viz. bones, gills, gut, intestine, kidney, liver, scales, skin, muscle and fats isolated for the determination of metal concentrations. The toxicity of aluminium, arsenic, barium and chromium to the three fish species viz. Catla catla, Labeo rohita and Cirrhina mrigala fluctuated significantly as a function of their diverse physico-chemical characteristics. The 60-day all the three fish species showed significantly higher sensitivity to all metals and their mixture while 240-day fish were significantly least sensitive. Catla catla were significantly more sensitivity to arsenic, followed by the sensitivity caused by metal mixture, chromium, aluminium and barium with the mean 96-hr LC50 values of 7.96 ± 1.40, 27.35 ± 9.17, 77.01 ± 22.47, 81.68 ± 28.54 and 112.45 ± 31.35 mgL-1, respectively. Labeo rohita were significantly least sensitive against barium (126.12 ± 22.76 mgL-1) while Cirrhina mrigala to aluminium with the mean concentration of 118.36 ± 28.91 mgL-1. Significant variations in the sensitivity of Catla catla, Labeo rohita and Cirrhina mrigala to various metals appeared species specific depending upon the nature of metals, exposure concentration and properties of water viz. water temperature, dissolved oxygen contents and pH. Exposure of aluminium to the fish caused significantly higher mean metal in the fish body, followed by that of barium, chromium and arsenic with statistically significant differences. Fish organs showed significantly variable ability to concentrate metals and therefore liver and kidney exhibited relatively high potentials for metals accumulation during short term (96-hr) acute exposures. All the three control (un-stressed) fish species exhibited significantly better growth due to significantly higher feed intakes than those grown under sub-lethal toxicity of either individual metals or metal mixture. Cirrhina mrigala gained significantly higher weight gains, followed by that of Catla catla and Labeo rohita under metal stress. All the three fish species showed significantly variable responses, in terms of increase in wet weights, fork and total lengths, feed intake and feed conversion ratios, due to chronic sub-lethal exposure to either individual metals viz. Al, As, Ba, Cr and mixture. However, all the three control (un-stressed) fish species exhibited significantly better growth due to significantly higher feed intakes than those grown under sub-lethal toxicity of either individual metals or metal mixture. The exposure of metal mixture (Al + As + Ba + Cr) caused significantly least growth, in terms of mean wet weights, in all the three fish species indicating a metal combination (Al + As + Ba + Cr) to be additive or synergistic in their mode of action in fish body. Among the three fish species, Cirrhina mrigala gained significantly higher weight gains, followed by that of Catla catla and Labeo rohita under metal stress. However, the difference between Catla catla and Labeo rohita for their growth rates was statistically non-significant. Therefore, lesser growth as a result of low feed utilization and feed conversion efficiency in fish, during sub-lethal exposure of metals and metal mixture, exhibited impaired normal 1 physiological functions in fish. Among the individual metals, chromium exposure caused significantly minimal effects on feed conversion efficiency of all the three fish species. The exposure of fish to individual metals viz. aluminium, arsenic, barium and chromium, during 90-day growth trials, caused significantly variable accumulation of these metals in the body organs of three fish species. However, fish liver, kidney and gills accumulated significant amounts of heavy metals. Three fish species also showed significantly variable responses for their tendencies to accumulate metals in their body organs. The exposure of metal mixture (Al + As + Ba + Cr) to all the three fish species caused significant accumulation of all metals in the fish liver, except arsenic in Labeo rohita. However, metal mixture exposure to all the three fish species caused significantly least accumulation of all metals in body fats. Among the three fish species, Cirrhina mrigala showed significantly higher ability to concentrate all the metals in their body organs.