Search or add a thesis

Advanced Search (Beta)
Home > Phytochemical and Biological Studies of Croton Bonplandianum Euphorbiaceae

Phytochemical and Biological Studies of Croton Bonplandianum Euphorbiaceae

Thesis Info

Access Option

External Link

Author

Qaisar, Muhammad Naeem.

Program

PhD

Institute

Bahauddin Zakariya University

City

Multan

Province

KPK

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Pharmacology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9443/1/Muhammad_Naeem_Qaisar_Pharmacy_2017_BZU_11.01.2018.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726915336

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


The research work was carried out for the phytochemical and biological studies of Croton bonplandianum (Euphorbiaceae). Preliminary phytochemical screening revealed the presence of alkaloids, saponins, flavonoids, tannins and terpenoids while anthraquinone glycosides and cardiac glycosides were absent. The extraction of dried plant material was affected by dichloromethane and methanol successively. Both dichloromethane and methanol extracts were subjected to biological activities such as antibacterial, antifungal, antioxidant, α-chymotrypsin inhibitory, urease inhibitory, α-glucosidase inhibitory and butyrylcholinesterase inhibitory activities along with brine-shrimp toxicity, phytotoxicity against Lemna minor. Dichloromethane extract has shown in vitro α-glucosidase inhibitory activity of 97.89 % with IC50 value of 14.93 μg/ml compared to the standard acarbose, which exhibited 92.23 % inhibition with IC50 value of 38.25 μg/ml. Methanol extract appeared with potent butyrylcholinesterase inhibitory activity of 84.14 % with IC50 found to be 31.01 μg/ml compared to the standard eserine, which exhibited 82.82 % inhibition with IC50 value of 30.01 μg/ml. Methanol extract was found toxic with LD50 value of 115.76 (0.0048 - 13.76) μg/ml against Artemia salina and also showed radical scavenging activity (%RSA) of 59.62% with IC50 value of 396.20 μg/ml . Based on these results activity guided isolation of constituents from dichloromethane and methanol extracts were done. Fractionation of dichloromethane extract by column chromatography on silica gel and Sephadex LH 20 using different mobile phase systems led to the purification of compounds (A-I). The structures of these isolated compounds were established by spectroscopic technique such as UV and IR spectroscopy. Proton Nuclear Magnetic Resonance (1H NMR), 13C NMR and Mass spectrophotometry (EIMS, HRMS) were used for elucidation of structure. On the basis of physical and spectral data from literature, these compounds were identified as n-pentacosanyln- nonadeca-7′-en-9′-α-ol-1′-oate (A), n-tridecanyl n-octadec-9,12-dienoate (B), nonacosyl hexadecanoate (C), heptacosanoic acid (D), 1,3,5-trihydroxy-2-hexadecanoylamino-(6e,9e)- heptacosdiene (E), coumarin (F), betulin (G), stigmasterol (H), and 3,5-dimethoxy 4-hydroxy cinnamic acid (I) were isolated. All these compounds were screened for in vitro α-glucosidase inhibitory activity, compound F, G and I possessed significant α-glucosidase inhibitory activity in a concentration-dependent manner and explained more potent inhibitory activity with IC50 values ranging from 23.0 to 26.7 μg/ml than that of a positive control acarbose (IC50, 38.2 6 μg/ml). Fractionation of methanol extract by column chromatography on silica gel using different mobile phase system afforded five compounds (J-N). Based on spectral data the chemical structure has been established as 4-hydroxy-3,5-dimethoxybenzoic acid (J), 5,8- dihydroxycoumarin (K), stigmasterol 3-O- β -D-glucoside (L), sparsifol (M) and 6-O-β-Dglucopyranosyl- β-D-(1-O-sinapoyl,6''-O-sinapoyl)-glucopyranose (N) were isolated from methanol extract of Croton bonplandianum. The compounds J, K, L and N exhibited significant butyrylcholinesterase inhibitory activity in a concentration-dependent manner and exhibited potent inhibitory activity with IC50 values ranging from 21.0 to 36.0 μg/ml, than that of a positive control eserine (IC50, 32.0 μg/ml).
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

خراجِ محبت

خراجِ محبت
(در صنعتِ توشیح)
ی
یورشِ کرب و بلا میں عزم کا کوہِ گراں
و
ورطۂ حیرت میں گم ہے علم و فن کا آسماں
ن
نازشِ اہلِ محبت، افتخارِ دوستاں
س
سرخوشی، وارفتگی کا ایک بحرِ بے کراں

ف
فصلِ گل میں ، حسن پرور ، گل رخوں کا ترجماں
ر
رشحہ فکر و نظر ہے کیف و مستی کا جہاں
ی
یاس نگری میں قسیمِ حوصلہ، ہمت نشاں
د
دشتِ نفرت کو قلم اس کا بنائے گلستاں
ی
یکہ تازِ فکر و فن ہے ، شاعرِ ندرت نشاں!
جمشید کمبوہ

عرب اسلامی روایت کے برصغیر پاک و ہند میں تفسیر نگاری پر اثرات: عہد رسالت تا خلافت عباسیہ کے تناظر میں اختصاصی مطالعہ

It is an established fact that the mainthrust driving force  of all Islamic disciplines is the personality of Prophet Muhammad (PBUH) who appeared in the Arab Peninsula, spreading the rays of divine wisdom throughout the globe. He emerged and prevailed the Prophetic message in such a vivid style that the whole humanity confessed his lasting impressions. The Indian sub-continent has also been one of the impressed territories despite of having no direct social, cultural or religious relationships, yet some approaches from Indian sub-continental Rajas and presentations of gifts to the Prophet (PBUH) have been recorded in the history. Historians have expressed the reality that before Muhammad bin Qasim, a numbers of companions of Prophet reached India and played a pivotal role in making the suitable ground for embracing Islam. Hence, the Arab epistemological tradition engraved in the core of Indian hearts which consequently, reappeared and emerged in the classical exegetical literature of sub-continental scholars, such as Shah Waliullah and his off shoots both in India and Pakistan. This paper has been specifically articulated to explore the Arab impression on exegetical writings in the sub-continent

Household Preferences and Willingness to Pay for Pesticides Free Fruit in Khyber Pakhtunkhwa Kp , Pakistan

The over use of fertilizers and pesticides in agriculture are degrading natural ecosystem and human health. The substantial association of health risk with pesticide residues and its adverse impact on environment has fetched attention of researchers across the globe. In this alarming scenario, pesticide free farming is deemed as panacea to environmental, human and animal health friendly production system. Pesticide free fruit are considered to be superior in quality as compared to fruit produced conventionally and helps in preventing several health and environmental hazards. Therefore, the demand for pesticide free fruit is increasing throughout the world. Given this increasing demand for pesticide free fruit, this study is designed to measure what price premium household would be willing to pay (WTP) for fruit free from pesticides such as apple, peach, and mango. This study also aims to identify factors influencing households‘ WTP decision of pesticide free fruit in Khyber Pakhtunkhwa (KP), Pakistan. The primary data was gleaned from 1000 sampled respondents through face to face interview from two selected districts namely Peshawar and Mardan KP, Pakistan. To elicit household‘s WTP information, Contingent Valuation Method (CVM) was employed. Contingent valuation is a survey based method used for eliciting willingness to pay of the households‘ for environmental and natural resources. Different elicitation techniques are used to design CV question scenario, such as payment card question, double dichotomous choice question and open ended question. Consequently, this study used the entire three payment format to elicit household‘s WTP for pesticide free fruit. The ordered logit model was employed to appraise the payment card method. However, linear regression model was adopted to identify factors influencing household‘s willingness to pay for pesticides free fruit. Nevertheless, the double bounded dichotomous choice model was performed to measure dichotomous choice questions. The major results demonstrate that 93.5 percent households were willing to pay higher prices for fruit pesticides free in the study area. Estimates remarkably reveal that around 35 percent households were willing to pay 16 to 20 percent higher prices and 24 percent households were willing to pay 6 to 10 percent higher prices for pesticides free fruit than the prevailing prices of conventional produce. It is reported by 61 percent of the households that local farmers use irrational pesticides on fruit. Major findings have also corroborated that 88.1 percent sampled household have perceived health concerns over pesticides residues in food. Similarly, 91.1 percent of the households have supported the idea that farmer should be engaged in environmental benign agricultural production practices which have a minimum adverse impact on health and the environment. Socio economic and demographic traits have confirmed positive significant effects on household‘s willingness to pay. The results of household‘s head awareness on WTP are according to the prior expectations that awareness of household‘s head has significant contribution in willingness to pay for pesticides free fruit. While household‘s size has a significant but inverse effect on willingness to pay for pesticides free fruit. Furthermore, household‘s perception regarding health and environment concern has a propensity to positively influence the willingness to pay for pesticides free fruit. It is also depicted by the results that mean WTP by using double dichotomous, payment card and open ended elicitation format validates consumer demand for pesticide free fruit in the study area. Three separate mean WTP were computed for each double dichotomous choice, payment card and open ended format which were figured as Rs.25.76, 16.32 and 14.37 respectively. These results reveal market potential for pesticides free fruit in the study area. This research study is expected to contribute in the three different aspects. Firstly, this study has figured out the potential for pesticides free markets in Pakistan. This study has also abridged the research gap regarding consumer‘s preferences and willingness to pay for pesticides free fruit which can be generalized and replicated to other areas. In addition, results have provided insight to fruit producers helping them to strategize production system decisions. Based on the findings from this research, government, nongovernment organization and other stakeholders should formulate policies and would promote and encourage the production of pesticides free fruit in Pakistan.