This dissertation consists of two parts; “Part A” and “Part B”. The “Part A” consists of phytochemical studies on three indigenous medicinal plants; Berberis brevissima Jafri, Berberis parkeriana Schneid and Berberis royleana Ahrendt. The selected species of Berberis have been investigated for the first time for their phytochemical constituents. Three new (tirahamine (65), 13-nitrotirahamine (67) and peshawarine (68)) and eighteen known compounds (berberine (69), dehydrocheilanthifoline (70), jatrorrhizine (71), berberrubine (72), 8-oxoberberine (73), columbamine (74), palmatine (75), glutamic acid (76), glutamic acid, methyl ester (77), di-glutamic acid (78), di-glutamic acid, methyl ester (79), di-glutamic acid, di-methyl ester (80), docosanoic acid (81), 23a- homostigmast-5-en-3ß-ol (82), β-sitosterol-3-O-β-D-glucopyranoside (83), nonacosane- 10-ol (84), palmitic acid (85) and linoleic acid (86)) have been isolated for the first time from these selected species. The three new bisbenzylisoquinoline alkaloids (tirahamine, 13-nitrotirahamine and peshawarine) have been isolated from B. brevissima. The spectral studies of the two of the compounds tirahamine and 13-nitrotirahamine were performed at different temperature using different solvents. Acetylation and nitration of tirahamine (65) have also been performed. In this part (Part A) we have described the isolation and structural elucidation of the three new and eighteen reported compounds. The structural characterization of the compounds have been achieved by using UV-Vis, IR, EI-MS, ESI-MS, 1 H NMR, 13 C NMR, DEPTq, DEPT-90, DEPT-135, HMBC, HSQC, COSY, NOESY and NOE techniques. xiIn the “Part B” of the dissertation anticancer, antidiabetic, antimicrobial, antitrypanosomal and nematicidal activities of the fractions and the compounds isolated from B. brevissima, B. parkeriana and B. royleana have been discussed. The anticancer activities were performed against six cancer cell lines i.e. L1210 (Murine lyphocytic leukemia), Colon 38 (Murine colon adrenocarcinoma), CFU-GM (Murine granulocyte macrophage colony forming unit), H-116 (Human colon adrenocarcinoma), H-125 (Human lung adrenocarcinoma) and CEM (Humane leukemic lymphoid). Berberine (69) and jatrorrhizine (71) showed significant activity and selectivity against L1210 and Colon 38 cell lines while palmatine (75) was inactive. The antidiabetic activities were performed against Protein Tyrosine Phosphatase 1B (PTP 1B) which is a negative insulin regulator. Amongst the tested compounds, 8-oxob- berberine (73) was significantly active and showed 29 % of the control. The other isolated compounds i.e berberine (69) (35 %), dehydrocheilanthifoline (70) (38 %), columbamine (74) (33 %) and jatrorrhizine (71) (36 %) also showed good activity while glutamic acid (76) (78 %) was inactive. Antimicrobial activities were carried out against four microbial strains i.e Mycobacterium marinum, sporadic methicillin resistant Staphylococcus aureus (SMRSA), endemic methicillin resistant Staphylococcus aureus (EMRSA) and Escherichia coli while antitrypanosomal against Trypanosoma brucei which is a protozoa belonging to the genus Trypanosoma, causing sleeping sickness. Tsetse fly is responsible for the transferring of this parasitic protozoa from infected human and animals. The Berberis brevissima roots xiifraction C (BBR-C) showed good activity against M. marinum (2.4 % of DMSO (D) control), high activity against SMRSA (0.2 % of D control) and EMRSA (0.2 of % D control) while against E. coli it was inactive. Berberine (69) showed good selectivity and activity against SMRSA and EMRSA (1.1 and 0.5 % of D control). Some of the fractions and compounds have also been tested for % mortality of stage two juvenile of root knot nematodes i.e Meloidogyne javanica. Amongst the various fractions, the Berberis brevissima roots fraction A (BBR-A) exhibited the highest mortality (62.22 %) followed by Berberis parkeriana roots fraction C (BPR-C) 57.22 % and BBR-C (54.00 %). Berberine (69) showed the highest nematicidal potential at 97.3 % of the standard (carbofuran) at the highest conc. (300 μg ml -1 ). Jatrorrhizine (71) ranked second and exhibited 59.50 % mortality followed by berberrubine (72) (49.17 %). Dehydrocheilnthifoline (70) was the least effective, nevertheless, showed good mortality at the highest conc.
علی اکبر ناطق اپنے بہن بھائیوں میں سب سے بڑے ہیں ان کے چھ بھائی ہیں۔ دو بھائی آرکی ٹیکٹ، کچھ کھیتوں میں کام کرتے ہیں اور کچھ نے اپنا خاندانی پیشہ ’’معماری ‘‘ جاری رکھا ہوا ہے۔ تمام بھائی ان سے چھوٹے ہیں۔ کل نو بہن بھائی ہیں ایک بہن تمام بہن بھائیوں سے چھوٹی ہے۔
ان کے چھوٹے بھائی اصغر جن کی وفات ایک حادثے میں ہوئی۔وہ اپنی بہن کے بارے میں بتاتے ہیں کہ ان کا نام خدیجہ تھا ،وہ سکول ٹیچر تھیں ،ان کا قتل ان کے شوہر نے کیا تھا اور ناطق وجہ بھی بتاتے ہیں کہ ان کے شوہر نے ان کا قتل انشورنس کے پیسوں کے لیے کیا تھا۔
هدفت هذه الدراسة إلى التعرّف على علاقة التفكير الإستراتيجي بالأداء الوظيفي في وزارة التربية والتعليم الفلسطينية ومديريّاتها، إضافة إلى التعرّف إلى واقع ممارسة أبعاد التفكير الإستراتيجي في الوزارة، ومعرّفة مستوى الأداء الوظيفي في الوزارة، كما تمثلت مشكلة الدراسة في ندرة الدراسات والبحوث التطبيقية التي تتناول التفكير الإستراتيجي ومستوى الاهتمام به وعلاقته بالمتغيرات الأخرى، خاصة في القطاع العام في فلسطين، ولتحقيق أهداف الدراسة استخدم المنهج الوصفي التحليلي، واستخدمت الإستبانة أداة لجمع البيانات اللازمة من مجتمع الدراسة وعدده 550 موظفًا، وقد اعتمدت العينة العشوائية الطبقية النسبية ، وقد توصلت الدراسة إلى مجموعة من النتائج أهمّها أنّ درجة تطبيق موظفي الإدارة العليا في وزارة التربية والتعليم للتفكير الإستراتيجي متوسطة، حيث بلغت نسبة تطبيق موظفي الإدارة العليا للتفكير الإستراتيجي (61.40%)، وأنّ مستوى الأداء الوظيفي لموظفي الإدارة العليا جاء بدرجة مرتفعة وبنسبة تقييم بلغت (70.8%) ، كما وتبين وجود علاقة طردية ذات دلالة احصائية بين تطبيق التفكير الإستراتيجي ومستوى الأداء الوظيفي في وزارة التربية والتعليم الفلسطينية. وفي ضوء النتائج التي توصلت إليها الدراسة، قُدمت مجموعة من التوصيات أبرزها على وزارة التربية والتعليم الفلسطينية تبني ثقافة التفكير الاستراتيجي ونشرها بين العاملين في كافة المستويات ومنحنهم صلاحيات تمكنهم من ممارسة التفكير الاستراتيجي. كما وعليها تعزيز العاملين ذوي الأداء الوظيفي المرتفع وتحفيزهم باستمرار ورصد احتياجاتهم وتدريبهم والذي ينعكس بشكل ايجابي في تحقيق أهداف وزارة التربية والتعليم الفلسطينية. الكلمات المفتاحية: التفكير الاستراتيجي ، الاداء الوظيفي ، التعليم في فلسطين
Elastomeric sponge and auxetic materials have unique characteristics which distinguish them from other solid materials like metals and ceramics. Such engineering materials with unique performance are a continuous requisite so as to assist the evolution for advance engineering applications. Auxetic materials (materials with negative Poisson’s ratio) have a unique feature of getting fatter when pulled and contract transversely when compressed, longitudinally. They are mainly used for their improved indentation resistance, higher fracture toughness, better thermal shock resistance, and good acoustic damping. Twenty diverse compositions were developed with various reinforcements’ incorporation such as carbon black nano particles etc., varying blending ratios of natural rubber (NR), styrene butadiene rubber (SBR), and nitrile butadiene rubber (NBR) in ethylene propylene diene monomer (EPDM). Sponge and auxetic structures were developed during vulcanization a hot isostatic biaxial hydraulic press by volumetric compression and cooling under pressure. The reinforcements were impregnated into the elastomeric matrices using internal dispersion kneader and two-rolls mixing mill. Seven types of mold geometries were designed and used as per ASTM standards to fabricate nanocomposites using a hot press in order to evaluate the elastomeric composites for rheological, mechanical, structural, vulcanization, compressive strain, dynamic mechanical thermal analysis and impact energy absorption applications. Mechanical characteristics were executed using Universal Testing Machine (UTM), Dynamic Mechanical Thermal Analyzer (DMTA) and rubber hardness tester. Scanning electron microscopy coupled with EDS were used to evaluate sponge and auxetic structures. The synthesized auxetic materials verified by scanning electron images and Poisson’s ratio determined by processing images using ‘Matlab’ software. With the advances in the fabrication and synthesis of a wider range of these thrilling materials, there is enormous potential for applications in industrial and commercial sectors. Among the various rubber systems investigated EPDM based elastomeric composites proved to be the best for development of auxetic structures. Hardness of the EPDM sponge composite with 20% incorporation of carbon black nano fillers enhanced up to 112%. EPDM with the addition of 20% carbon black attained tensile strength 142%, compared to EPDM without reinforcement; this nanocomposite also showed a 20% reduction in impact energy absorption. EPDM-30%NR reinforced with 20% carbon black showed a minimum reduction of 33% in storage modulus indicating good retention of elasticity. Maximum enhancement of 400% loss modulus was obtained in case of EPDM-30%SBR reinforced with 20% carbon black. Rheological bahaviour such as loss tangent during vulcanization enhanced up to 130%, 300%, 85%, 11% by incorporation of 30% carbon black, 30% NBR, 30% NR, and 30 % SBR.