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Phytochemical and Pharmacological Investigations of Quercus Incana

Thesis Info

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Author

Gul, Farah

Program

PhD

Institute

University of Peshawar

City

Peshawar

Province

KPK

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Pharmacy

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12340/1/Farah%20Gul_Pharmacy_2017_UoPsw_PRR%20personally_HS%20Diff.docx

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726917139

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The present study was aimed to extract, fractionate, isolate and standardize the chemical constituents from the plant, Quercus incana, in order to find out their phytochemical and pharmacological mode of action, so as to discover a new source for potentially active constituents used for the benefits of mankind. Quercus incana, which is a large evergreen tree belongs to family Fagaceae. The Fagaceae is a large plant family found in Asia, North America and Europe. Quercus incana has been used in different ailments such as diarrhea, neurosis, pyrexia, dysentery, pain, inflammation and burns healing in conventional medicines. It is also employed to treat gonorrhea and used as an astringent, diuretic, and various nervous disorders all over the world including Pakistan. In the present research work, the plant Quercus incana was explored for its chemical constituents using chromatographic and spectroscopic techniques for potentially new and therapeutically active agents. The bark portion was selected for isolation, on the basis of preliminary pharmacological activities and its common use by the local people and Hakims and Tabibs in different ailments. Some activities were performed on fruit extract also. Crude extract of bark and fruit of Quercus incana and the new compound isolated from bark were screened for various in-vitro and in-vivo biological activities like antibacterial, anti oxidant, analgesic, anti-inflammatory etc, inorder to give scientific background to various ethnomedicinal applications of the plant which will lead to safe, therapeutic, effective and economical way of treatment of different diseases and to examine and analyze the chemical composition of the plant. For the isolation of pure secondary metabolites, the ethyl acetate fraction (obtained from crude methanolic extract of the bark) was subjected to column chromatography. The structures of purified compounds were elucidated by using various sophisticated spectroscopic techniques i.e, 1H-NMR, 13C-NMR, COSY, NOESY, DEPT, HSQC, HMBC, UV, IR and EI-MS. The plant was found to be a new source of six compounds such as, betulinic acid, 9-octadecanoic acid methyl ester(z-form), gallic acid, gallic ester, b-sitosterol glucoside, and quercitin. Three known compounds like β-sitosterol, ursolic acid, and lupeol were also isolated. Quercuschin, which was a completely new compound was discovered for the first time from this plant source. Results of in-vivo biological studies reveal that the plant possesses very good property of producing peripheral and central analgesia. The data obtained from different experiments supports the use of the plant in relieving fever, and treating inflammatory conditions. The different in-vitro activities of new compound were conclusive of the fact that the plant is a good natural antioxidant and can be used to treat various bacterial and fungal infections. The isolation work done on the plant (bark) of Quercus incana yielded 10 compounds. In these 10 compounds, 7 were reported for first time from this plant species : THESE WERE , betulinic acid, 9-octadecenoic acid methyl ester(z-form), gallic acid, gallic ester, b-sitosterol glucoside,quercitin , and one compound NAMELY Quercuschin: was absolutely new and was never reported before from any source AND was discovered for the first time from this plant source. 3 compounds were KNOWN compounds: NAMELY β-sitosterol, ursolic acid, and lupeol, Findings of this research project, especially the biological activities of chemical constituents of the plant, strongly support the folkloric uses of the plant and its applications in traditional treatment. The isolated compounds strengthen these activities. However, further comprehensive research work on the crude extract as well as on isolated compounds is needed to explore and identify lead compounds of clinical effectiveness for the development of new drugs in order to strengthen the field of drug discovery.
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شہادت حضرت امام حسین رضی اللہ تعالیٰ عنہ

شہادت حضرت امام حسین ص
نحمدہ ونصلی علی رسولہ الکریم امّا بعد فاعوذ بااللہ من الشیطن الرجیم
بسم اللہ الرحمن الرحیم
معزز اساتذہ کرام اور میرے ہم مکتب شاہینو!
آج مجھے جس موضوع پر اظہار خیال کرناہے وہ ہے:’’شہادت حضرت امام حسین ؑ‘‘
غریب و سادہ و رنگیں ہے داستانِ حرم
نہایت اس کی حسینؓ، ابتدا ہے اسمٰعیلؑ
جنابِ صدر!
حضرت امام حسین ؓحضرت رسول کریمؐکے نواسے حضرت علی ؑکے لختِ جگر حضرت فاطمہؓ کے جگر گوشے اور حضرت امام حسن ؓکے بھائی تھے۔ یہی حسنین کریمینؓ سرور کائنات ؐ کی آنکھوں کی ٹھنڈک اور دل کا سکون تھے۔ محبوبِ خدا ؐان کے لیے اپنے سجدے طویل فرماتے ان کا رونا آپؐ پر گراں گزرتا۔ حضرت حسین ؓکے متعلق آپؐنے ارشاد فرمایاحسینؓ مجھ سے ہے اور میں حسینؓ سے ہوں۔ خدا اسے دوست رکھے جوحسین کو دوست رکھے۔
عزیز ساتھیو!
حضرت حسین ؓنے میدانِ کربلا میں بے مثال شجاعت، بہادری اور ایثار و قربانی کا مظاہرہ کیا آپ ؓنے اپنے اور اپنے اہل وعیال کے خون سے شجرِ اسلام کو سینچا۔آپ ؐنے اسلام کی حرمت اور بقا کی خاطر اپنا سرتو کٹوادیامگر باطل کے سامنے جھکے نہیں آپ ؓنے دنیا کے سامنے صبر ورضا اور قربانی کا ایسا نمونہ پیش کیا جس کی نظیر رہتی دنیا تک مشعل حق بن کر جگمگاتی رہے گی۔
یا حسینؓ ابن علیؑ سب پر تِرا احسان ہے
وہ تِرا ممنون ہے جو با ضمیر انسان ہے
جنابِ صدر!
یزید لعین ہر قیمت پر حضرت امام حسینؓسے بیعت لینا چاہتا تھا مگر سیدنا حسین ؑیزید کو خلافت کے اہل نہیں سمجھتے تھے۔ آپ ؓتدبر، تقویٰ اورعلم و حلم کے جس اعلی مقام پر فائز تھے اور پوری امت مسلمہ کی نظر یں آپ پر لگی ہوئی تھیں، خلافت کے لیے عدالت و تقویٰ کے جس معیار کی...

تشکیل معاشرہ میں تصوف کی بنیاد اخلاق حسنہ کا کردارایک تحقیقی مطالعہ

Almighty sent his chosen prophets to every Ummah to teach them the lesson of goodness and to avoid evil. The purposes of the Prophet (peace and blessings of Allah be upon him) were reform processes: to teach and guide, and pushed the people to Almighty Allah. According to the narrative in Islamic law, everyone's either Muslim or non-Muslim, relative or/neighbor has determined the right to live things. And good conduct is that because Abdullah bin Mubarak said That is, "Meeting old habits, doing righteousness, and forbidding people to hurt you". If human behavior is good then its own life will also pass through fortune and joy, and it will also be the mercy and chain of things for others. Collective relations, interacting with the recipients, and the Family system, is proven to be a source of intelligence. The result of the happy moral is Reality and Heaven of Arman al-Rahman, and the fulfillment of the Prophecy is the wrath of Lord Koran and the Fire of Hell.  

Developing a Hybridized Chicken for Rural Poultry

The present breeding study was planned to cross the various indigenous breeds for developing a hybrid rural breed which can live as a scavenger bird under the harsh rural conditions with improved economic traits. The study was conducted in three independent phases. In first phase, breeds comparison was performed for Desi, Fayoumi and Rhode Island Red chickens. Maximum hatchability was recorded in Fayoumi followed by RIR and Desi. The average day old weight was seemed highest in RIR, intermediate in Desi and lowest in Fayoumi. The poor feed conversion was observed in Desi breed and better feed conversion was recorded in RIR breed. The early age of sexual maturity was recorded in Fayoumi followed by RIR and Desi. The RIR chickens had highest egg production followed by Fayoumi and Desi chickens. The all internal egg quality parameters were significantly higher in RIR than Fayoumi and Desi breeds except shell and yolk thickness. In second phase, the performance of RIR and Fayoumi chickens were examined with their reciprocal crossbred chicks. Higher hatchability was noticed in crossbred chickens than their parents. The average day old weight was highest in RIR and FIRI (Fayoumi male × RIR female) followed by RIFI (RIR male × Fayoumi female) and Fayoumi. The average weight gain and daily feed intake was higher in FIRI chickens than RIFI crossbred chickens. The better FCR and liviliability were observed in both crossbred chickens during growing phase. Morphologically the two crossbred chicken (RIFI and FIRI) showed variation in all characters. The dominant skin colour for both crossbred chickens was white while the egg shell colour was chalky white. The major feather colour in RIFI was brown with xxblack spots, while black & white in FIRI. Shank colour was 100% yellow in RIFI and 100% black in FIRI. The dominant comb type in the whole ecotypes was the single one. The heterotic effects on body weights were significant in both crossbred chickens except at day old and gradually increased to 10.27% at 3 weeks in RIFI and 19.45% at 8 weeks in FIRI and then declined to 5.50% at 4 weeks in RIFI and 8.70% at 9 weeks of age in FIRI. Results for reciprocal crosses in this research study revealed that FIRI had high heterotic percentage at 10 weeks of age to get hybrid vigor in growth traits. These results may helpful for the poultry breeders to cross these two breeds (Fayoumi X RIR). The egg production was highest in FIRI followed by RIFI, RIR and Fayoumi chickens respectively. In conclusion, the crossbred chickens of Fayoumi male and RIR female (FIRI) showed better performance in all traits than crossbred chickens of RIR male and Fayoumi female (RIFI). On the basis of above results, crossbred females of FIRI were retained and mated to the third breed (Whitel Leghorn) for further improvement in production performance in the subsequent trial. In third phase, crossbred chicken with proposed name; Rural Leghorn (RLH) breed was developed by crossing White Leghorn male with FIRI females. The progeny as RLH chickens contain White Leghorn (50%), RIR (25%) and Fayoumi (25%). In this phase, according to research plan, only growth data regarding RLH-G1was also recorded and compared with performance of FIRI, RIFI and RLH breeds. The average day old weight was highest in RLH followed by RLH-G1, FIRI and RIFI. The results showed that RLH and RLH-G1 crossbred chickens had lowest mortality and were shown heavier in weight at 20 weeks of age. The dominant skin colour for the crossbred chicken was white for RLH and xxiRLH-GI while the egg shell colour was light brown in RLH. The major feather colour in RLH was white whereas in RLH- G1 ratio of white and colored birds was 75:25 respectively. Shank colour was 100% yellow in RLH whereas 84% white in RLH-G1. The heterotic effects on body weights were significant in both crossbred chickens except at day old and gradually increased to 8.55% at 7 weeks in RLH and 26.5 in RLH-G1 and then declined to 4.28% at 9 weeks in RLH where as 22.67 in RLH-G1. The highest egg production was found in RLH (72.2%) followed by FIRI (60%), RIFI (54%), RIR (41%) and the Fayoumi chickens (36%). As far as the rate of lay (no. of eggs per hen) is concerned, RLH scored higher (253) than those of FIRI (198), RIFI (178), RIR (141) and Fayoumi (123) chickens. In conclusion, newly evolved RLH chicken gained better body weight than Fayoumi and Desi, moderate than RIR and FIRI chickens with lower mortality. The RLH chicken showed better performance in all egg traits than parents and other crossbred chickens of RIR male and Fayoumi female. The growth performance of RLH- G1chicken was better than reciprocal crosses of RIR and Fayoumi chicken and at par with RLH chicken.