Present research work is based on nutritional, biological and antimicrobial investigation of wild edible plants of Hazara region of Pakistan. Ethnobotanical information was obtained from available literature and inhabitant of Mansehra, Abbottabad and Haripur district. The plants samples were analysed for nutritional components (carbohydrates, fats, proteins, fibers, ash and energy vale), selected metals (Ca, Mn, Fe Mg, K and Na), phytochemical constituents (phenolics, flavonoids, flavonols and ascorbic acid) and antioxidant activity (DPPH scavenging activity) by using standard analytical methods. A total of 3 wild edible plant species including Malva neglecta, Pimpenela stewartii and Viola odorata were investigated in this study. Elemental analysis (mg/kg, dry weight) indicated that Ca exhibited highest average levels, followed by K, Mg and Na while lowest levels were observed for Mn and Fe in the Pimpenella stewartii, Malva neglecta and Viola odorata samples. Their concentrations were varying to different samples. The plants found rich in phytochemical contents and had good antioxidant activities. Significantly higher phenolics, flavonoids, ascorbic acid contents and antioxidant activities were observed in Pimpenella stewartii. The higher concentration of alkaloids was found in Pimpenella stewartii than Viola odorata. The Pimpenella stewartii and Malva neglecta have higher concentration of flavonoids than Viola odorata. The phenolic compounds were found in Pimpenella stewartii and Viola odorata in higher concentration than Malva neglecta. Malva neglecta has relatively low amount of saponins than Pimpenella stewartii and Viola odorata. The Diterpenoids and Triterpenoids were present in all the extracts of Pimpenella stewartii, Malva neglecta and Viola odorata in considerable amounts. Glycosides and Cardiac glycosides were found only in Malva neglecta in low quanitity. Coumarins were also present in Malva neglecta in good quantity but absent in other two plants whereas, tanins were not present in any of three selected plant species. The moisture content was highest in Pimpenella steawartii (9%) and lowest in Viola odorata (6.9 %) and Malva neglecta (6.5%). The ash content was highest in Malva neglecta (17.5%) followed by Viola odorata (13.2%) and Pimpenella stewartii (10.4%). The crude protein content was 5.51%, 4.37%, 3.31% in Pimpenella stewartii, Malva neglecta and Viola odorata respectively. Crude fiber content was found highest in Viola odorata (23.20%) followed by Malva neglecta (21.5%) and lowest in Pimpenella stewartii (12.19%). Lipid content was highest in Viola odorata (12.10%), low in Pimpenella stewartii (3.20%) and lowest in Malva neglecta (2.65%). Carbohydrate content was highest in Pimpenella stewartii (61.9%) and lowest in Malva neglecta (28.70%) and Viola odorata (28.30). The free radical scavenging activity of all the extracts and standard ascorbic acid at lowest concentration of 62.5 ppm and highest 1000 ppm concentrations follows the general order of Ascorbic acid> Pimpenella stewartii> Viola odorata>Malva neglecta. Minimum IC50 value for antioxidant activity was demonstrated by ascorbic acid (< 10 ppm) followed by Malva neglecta (157 ppm), Pimpenella stewartii (205 pmm) and Viola odorata (260 ppm) respectively.The comparative efficacy of the crude, methanolic, ethanolic and phenolic extracts of Pimpenella stewartii was investigated against E.coli, Staphylococcus aureus, Bacillus subitilis, Kleibshiela spp., Clostridium tetani and Mycobacterium spp. Ethanolic extract of Pimpenella stewartii showed highest zone of inhibition against Staphylococcus aureus (16.67mm) and lowest was found in methanolic extract against Kleibshiela spp (6.7 mm) as compared to control (19mm). The crude extract of Malva neglecta showed highest activity (18.3) against E.coli and lowest (7mm) against B.subtilis as compared to control (21.33). Crude extract of Viola odorata showed highest zone of inhibition (18.67mm) against Mycobacterium and lowest (7.3mm) against Kleibshiela spp as compared to control (20.33). The antimicrobial activity of Viola odorata was also compared with Balsamodendron myrrha against Klesbella pneumonia, E.coli, Pseudomonas, Salmonella typhi, Staphylococcus epidermidis and Enterococcus. The highest antifungal activity (18.60mm) was showed by Viola odorata in ethanolic extract against Aspergillus niger and lowest activity of Viola odorata was found in crude extract (11.00mm) against Mucor hiemalis. The Malava neglecta showed highest antifungal activity (18.00mm) in crude extract against Aspergillus niger and lowest activity in crude extract (6.00mm) against Mucor hiemalis. Pimpenella stewartii showed highest antifungal activity (16.67mm) in methanolic extract against Aspergillous flavus and ethanolic extract against Aspergillus niger, whereas lowest activity of Pimpenella stewartii was found in methanolic extract (6.7mm) against Mucor hiemalis. The Viola odorata exhibited alpha-glucosidase inhibitory activity (upto 72.0%), and alpha-amylase enzyme (upto 45.7%) at concentration of 1mg/ml followed by Malva neglecta extract which showed inhibitory effect on alpha-glucosidase enzyme (upto 58.50.0%), and alpha-amylase enzyme (upto 38.30%) at concentration of 1mg/ml. The minimum antidiabetic activity was observed in Pimpenella stewartii which showed inhibitory effect on alpha-glucosidase enzyme (upto 16.76.0%), and alpha-amylase enzyme (upto 32.9%) at concentration of 1mg/ml. All the three plants Pimpenella stewartii, Malva neglecta and Viola odorata showed good brine shrimp larvicidal activity. Maximum mortalities (76.6%) were observed at a concentration of 1 ppm in Malva neglecta followed by 63.66 % at a concentration of 100 ppm in the same plant. The lowest value was found 36.66% at a concentration of 100 ppm of Viola odorata. Among the three plants extracts, Malva neglecta showed the highest growth inhibition of lemna minor followed by Viola odorata at the same dose whereas Pimpenella stewartii showed the lowest mortality of Lemna minor.
سیٹھ جمال محمد دوسرا قومی حادثہ مدراس کے مشہور اور مخیر سیٹھ جمال محمد کی وفات ہے مسلمانوں میں صاحبِ ثروت تاجروں کی کمی نہیں لیکن مرحوم کے اوصاف و خصوصیات کی مثال مشکل سے ملے گی، دولتِ دنیا کے ساتھ اﷲ تعالیٰ نے ان کو دینداری کی دولت بھی عطا فرمائی تھی، اور ان کا دل ملک و ملت کی محبت سے بھی معمور تھا، انہوں نے بڑی دولت پیدا کی اور اسی فیاضی سے اس کو قوم و ملک کی راہ میں صرف کیا، ہندوستان میں مسلمانوں کی کوئی ایسی تحریک نہیں تھی جس میں ان کی امداد شامل نہ رہی ہو، مذہبی اور تعلیمی کاموں سے خصوصیت کے ساتھ بڑی دلچسپی تھی، ندوۃ العلماء لکھنو، دارالعلوم دیوبند، مدرستہ العلوم علی گڑھ، مسلم یونیورسٹی آل انڈیا مسلم ایجوکیشنل کانفرنس، جامعہ ملیہ اور اس قسم کے تمام دوسرے اداروں کے وہ معاون و مددگار تھے، شہر مدراس اور اس کے مضافات میں کئی عربی مدرسے اپنے صرف سے چلاتے تھے، مدراس میں انگریزی خواں مسلمان طالب علموں کے لیے ایک ہوسٹل بنوایا جس میں ان کی مذہبی تعلیم و تربیت کا بھی انتظام تھا، سیکڑوں غریب طالب علموں کو وظائف دیتے تھے، حضرۃ الاستاد مدظلہ کے خطبات مدراس، محمد مارماڈیوک پکتھال اور سراقبال مرحوم کے انگریزی خطبات بھی مرحوم ہی کے جذبہ دینی کی یادگار ہیں۔ مرحوم کو سیاسی کاموں سے بھی دلچسپی تھی، تحریک خلافت میں ان کا بڑاحصہ تھا، اس میں انہوں نے ایک لاکھ کا عطیہ دیا تھا، ایک زمانہ تک کانگریس کے بھی سرگرم رکن رہے ،لیکن پھر سیاست سے الگ ہوگئے تھے، اپنی زندگی میں انہوں نے لاکھوں روپیے دین و ملت کی راہ میں خرچ کئے، مدراس میں ان کا دولت کدہ اہل حاجت کا ملجا و مادیٰ تھا، لیکن اس دولت و ثروت کے ساتھ خود ان کی زندگی...
هدفت هذه الدراسة إلى التعرّف على علاقة التفكير الإستراتيجي بالأداء الوظيفي في وزارة التربية والتعليم الفلسطينية ومديريّاتها، إضافة إلى التعرّف إلى واقع ممارسة أبعاد التفكير الإستراتيجي في الوزارة، ومعرّفة مستوى الأداء الوظيفي في الوزارة، كما تمثلت مشكلة الدراسة في ندرة الدراسات والبحوث التطبيقية التي تتناول التفكير الإستراتيجي ومستوى الاهتمام به وعلاقته بالمتغيرات الأخرى، خاصة في القطاع العام في فلسطين، ولتحقيق أهداف الدراسة استخدم المنهج الوصفي التحليلي، واستخدمت الإستبانة أداة لجمع البيانات اللازمة من مجتمع الدراسة وعدده 550 موظفًا، وقد اعتمدت العينة العشوائية الطبقية النسبية ، وقد توصلت الدراسة إلى مجموعة من النتائج أهمّها أنّ درجة تطبيق موظفي الإدارة العليا في وزارة التربية والتعليم للتفكير الإستراتيجي متوسطة، حيث بلغت نسبة تطبيق موظفي الإدارة العليا للتفكير الإستراتيجي (61.40%)، وأنّ مستوى الأداء الوظيفي لموظفي الإدارة العليا جاء بدرجة مرتفعة وبنسبة تقييم بلغت (70.8%) ، كما وتبين وجود علاقة طردية ذات دلالة احصائية بين تطبيق التفكير الإستراتيجي ومستوى الأداء الوظيفي في وزارة التربية والتعليم الفلسطينية. وفي ضوء النتائج التي توصلت إليها الدراسة، قُدمت مجموعة من التوصيات أبرزها على وزارة التربية والتعليم الفلسطينية تبني ثقافة التفكير الاستراتيجي ونشرها بين العاملين في كافة المستويات ومنحنهم صلاحيات تمكنهم من ممارسة التفكير الاستراتيجي. كما وعليها تعزيز العاملين ذوي الأداء الوظيفي المرتفع وتحفيزهم باستمرار ورصد احتياجاتهم وتدريبهم والذي ينعكس بشكل ايجابي في تحقيق أهداف وزارة التربية والتعليم الفلسطينية. الكلمات المفتاحية: التفكير الاستراتيجي ، الاداء الوظيفي ، التعليم في فلسطين
Abiotic stress is a natural part of every ecosystem that adversely affects the population‘s performance or individual physiology of the organism in a significant way. It is essentially unavoidable. Abiotic stressors are most harmful when combined with other stresses. These stresses alter morphological, physiological, biochemical, metabolic and molecular parameters. Plants, in general, have the ability to cope with these stressful conditions. They do so by triggering a complex set of defense system to survive and maintain growth. Mechanisms of these events are however, not yet clearly understood. To explore intricacies of stress tolerance processes we need to examine natural phenomenon of plant survival under harsh environments such as arid and semi-arid locals. NaCl or salinity stress is just one among abiotic stresses present around. The project being presented has been conceived with the idea of investigating salinity stress tolerance in Vetiveria zizanioide, a hardy grass, which grows abundantly under the harsh environmental conditions of a nearby arid landmass, the Cholistan desert. During the present study two ecotypes (E1 & E2) of Vetiver grass were exposed to varying levels of NaCl stress (0 to 350 mM). Various morphological features including plant health, plant height, shoot length, total number of tillers, no. of nodes on main stem, no. of internodes on main stem, average leaf area, root health and root length were examined. It was seen that on exposure to salt stress most of the E2 plants exhibited better health as compared to E1 plants. However, in both ecotypes 71% of the plants were of green color indicating healthy status of plants. Rest of the plants was either yellowish green (14.29 %) or pale yellow color (14.29 %) due to adverse effects of high salt concentrations. Better health status of E2 plants was also depicted by higher plant height, shoot length and root length observed in case of E2 plants as compared to E1 plants when treated with 200 mM NaCl. Overall morphological results indicated that E1 is more susceptible while E2 is more tolerant ecotype. Morphological analysis was followed by physico-chemical analysis that included measurement of moisture contents (%), dry mass (%), Electrical conductivity (EC), pH, sodium contents and chloride contents. In both ecotypes of hydroponically grown Vetiver plants, at higher salt concentrations % dry mass was higher in shoots followed by leaves and roots. It is obvious that EC of the nutrient solution increases with the increasing levels of NaCl. A gradual increase in EC was seen with increasing NaCl concentration from T1 to T6 as compared to control. Our results of Na and Cl estimation of plant tissues showed that almost all tissues (leaves stem and root) showed a gradual increase in Na and Cl contents as NaCl concentration increased. This verified that under salt stress all tissues had accumulated a considerable amount of Na and Cl contents. We then documented expression profile of three enzymes guaiacol peroxidase, amylase and catalase and their isozymes, , through native PAGE using enzyme specific staining methods. Level of enzymatic activity was accessed by performing enzyme specific quantitative assays. All morphological and biochemical data were analyzed by ANOVA (one-way analysis of variance) employing Post Hoc multiple comparisons test and Independent samples T-test. Moreover, the average leaf area, plant height, shoot length, root length, sodium contents, chloride contents, and specific activity of three studied enzymes were initially increased under low salt concentration (25 mM to 150 mM NaCl) then at high saline conditions beyond 150 mM NaCl, a sharp declined was observed and both ecotypes exhibited statistically significant variations in almost all parameters (P< 0.0005 or 0.005). Variations in expression of these enzymes in different tissues indicated that these tissues sense and hence are exposed to different levels of NaCl stress. They probably adopt different strategies to generate an effective and beneficial response. It is known that these strategies may include comprised of ‘switching on’ or ‘switching off’ the corresponding genes, enhancing or decreasing their levels of expression, generating a variety of isoforms, and coordinated and sequential expression of the related genes. Vetiver plants were analysed to document expression profile of three enzymes (Guaiacol peroxidase, amylase and catalase) and their isoforms in both ecotypes. Densitometric analysis was done by gel image analyzer software. The result of densitometric analysis depicted that 8 isoforms of guaiacol peroxidase (GPX), seven isoforms of amylase and seven isoforms of catalase (CAT) were identified in roots, shoots and leaves of both E1 and E2 when exposed to NaCl salt stress. In almost all cases the expression pattern of isoforms was dependent on salt concentration. Different isoforms were expressed at different salt concentrations. Thus each ecotype develops a unique pattern of response and hence the related genetic variations. This research has provided insight in the stress tolerance process. The information thus obtained demands exploring these responses at molecular and gene levels. Once delineated these genetic components could help to develop transgenic commercial varieties for cultivation in arid land masses such as Cholistan.