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Home > Polycyclic Aromatic Hydrocarbon Sequestration by Soil Organic Matter Fractions and its Effect on Plant Growth

Polycyclic Aromatic Hydrocarbon Sequestration by Soil Organic Matter Fractions and its Effect on Plant Growth

Thesis Info

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Author

Nisa, Waqar-Un-

Program

PhD

Institute

Pir Mehr Ali Shah Arid Agriculture University

City

Rawalpindi

Province

Punjab

Country

Pakistan

Thesis Completing Year

2015

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/6878/1/Waqar_un_nisa_Environmental_Sciences_2015_PMAS_Rwp.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726961542

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Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental hazards in Pakistan. There are many ways in which the PAH can enter the soil environment and interfere with the soil system and the plants grown in soil. This study demonstrates the efficacy of organic amendments to improve phytoremediation efficiency in PAH contaminated soil. The main objectives of the study were to compare PAH concentration in soil before and after the pot experiments in different treatments and demonstrate the interaction of soil PAHs and compost amendments on PAH bioavailability in soil. To assess plant growth in PAH contaminated soil and their subsequent uptake of PAH by vetiver grass and rye grass and to evaluate the effect of season on the PAH sequestration in SOM fractions. Experiments were conducted with two different soils (S1: Gujar Khan with silty clay loam texture and S2: PMAS-Arid agriculture university Rawalpindi main campus with sandy loam texture) and grass (P1: vetiver; P2: rye grass) types. Each type of soil and grass was studied in six different levels of diesel contamination and compost amendment (T1: Control; T2: 1% compost; T3: 0.5% diesel (PAHs); T4: 1% diesel (PAHs); T5: 0.5% diesel (PAHs) + 1% compost and T6: 1% diesel (PAHs) + 1% compost) and performed with three replications. Pot trials were conducted in two seasons: during June – September (2012), and October – January (2013). Soil physic-chemical analysis and soil organic matter fractionation was performed at the start of experiments and also at the end of pot experiments. Soil PAHs analysis was done after the 15 days of spiking and at the end of experiments. PAHs were analyzed by GC-MS. Physicochemical analysis of the soil pH and EC values were lower than the initial values. 21 Similarly organic matter, TOC and TN concentrations varied significantly in each treatment. Plant growth revealed that PAH contamination negatively influenced both grass species. However adding compost improved the plant growth in PAHcontaminated soils with 1% compost. In treatments with diesel and compost amendments the plant biomass was higher in summer compared to winter. A 56% decrease in root length was observed in vetiver grass when soil was spiked with 1% diesel. Uptake of low molecular weight PAHs was higher compared to high molecular weight PAHs. Accumulation of PAHs in root and shoot corresponded to the removal of PAHs from soil by grasses. The accumulation of PAHs in plant biomass was greater in summer than winter. The effect of various treatments applied showed that the more PAHs accumulated in the absence of compost. Whereas the sequestration by SOM in T5 and T6 reduced the concentration of PAHs in soil. In vetiver most of PAHs are accumulated in roots compared to shoots. GC-MS analysis for soil PAHs indicated that PAH concentration declined from the initial concentration. Microbial community analysis by TRFLP showed that Streptomyces and Mycobacterium were the dominating species in diesel contaminated soil. Soil fractionation showed that the humin fraction had higher percentage in both types of soils, while Fulvic acid concentration was lowest in all treatments. However, fulvic acid content was positively correlated to the PAHs in soil. In the case of high molecular weight PAHs, humic acid was positively correlated with the sequestration matrix. This study clearly showed that the phytoremediation of contaminated soil using organic amendments and plants with a dense root system could be a useful approach for removal of PAHs from contaminated soil. Compost amendment has increased the degradation of PAHs through facilitated by microbial activity. Soil organic matter fractions were the prime sites where PAHs was mostly sequestered.
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کہتے ہیں جب سقراط کو زہر کا پیالہ دیا گیا اور اس نے مسکراتے ہوئے اسے اپنے ہو نٹوں سے لگاناچاہا تو اس کے ایک شاگرد نے زار قطار روتے ہوئے کہا ’’استاد مجھے افسوس ہے کہ آپ بے گناہ مارے جائیں گے‘‘سقراط نے زہر کے پیالے کو ذرا اور مضبوطی سے پکڑتے ہوئے کہا میں بے گناہ ضرور مارا جائوں گا لیکن میری یہ بے گناہی مجھے تاریخ میں ہمیشہ زندہ رکھے گی ۔تم اپنا مشن جا ری رکھنا ‘‘حقیقت بھی یہی ہے کہ ابدی زندگی انہی کو نصیب ہو تی ہے جو بے گناہ مارے گئے یا جو کسی عظیم مقصد کی خاطر اپنے نظریے کی صداقت کا پرچم بلند رکھتے ہوئے اپنے خالقِ حقیقی سے جا ملے ،
یہ تاریخ کا فیصلہ ہے کہ آزاد منش لوگوں نے اپنی آزادی کے لیے ہمیشہ رسم دار کو زندہ رکھا ۔ہر زمانے میں کوئی نہ کوئی منصور ضرور پیدا ہو اجس نے اپنے عہدِ وفا کو نبھانے کے لیے اس رسم کو زندہ رکھنے کے لیے یہ علان کیا کہ :
ہم ہیں منصور اس زمانے کے
ہم سے ہی رسمِ دار زندہ ہے
انسانی تاریخ کا جائزہ لیا جائے تو یہ بات واضح ہو جاتی ہے کہ ہنسی خوشی زندگی قربان کرنے کا جذبہ اور سر فروشی کی رسم کے پس منظر میں دو محرکات بہت نمایاں رہے ایک تو مذہب اور دوسرا سیاسی فلسفہ ۔ انسانی وقار اور تقدیس کو زندہ رکھنے والی سوچ اور فکر کی آزادی ضمیر کی آواز کی سر بلندی کے لیے جن لو گوں نے کسی سیاسی پلیٹ فارم پر جد وجہد کی انسانی قدرو ں کی پاسداری اور حقیقی جمہوری معاشرے کے لیے جدو جہد کی بلا شبہ تاریخ انسانی میں ان کا نام ہمیشہ زندہ رہے گا ۔
سر فروشی کے اس قبیلے...

Factors Affecting the Academic Achievements among Dean’s Listers of Caraga State University

The study assessed the relationship between the factors affecting the academic achievement of the dean’s listers’ of Caraga State University. It involves the total population of the dean’s listers in the said university. The independent variables are those pre-determined factors’ affecting the academic achievement of the dean’s listers’ of Caraga State University and the dependent variable is the grades of the dean’s listers’. The result shows the low relationship between the pre-determined factors and the academic achievement evidenced by the values of the p-values which are greater than. In terms of the academic achievement of the dean’s listers’ their grades signifies their excellence in their different chosen fields. With regards to the pre-determined factors, the factor that got the highest mean is the teachers’ competence with 3.7639 and the lowest one is the learning environment with 3.6690. The study habits’ got the second spot among the 4 factors followed by the learning styles. Based on Spearmen Correlation analysis in the data gathered, the results revealed that there is no significant relationship between the pre-determined factors and the academic achievement of the dean’s listers’ of Caraga State University. The p-values obtained are less than 0.05 for all the data set; that is accepting the null hypothesis. The results clearly depicts that the students’ study habit, learning style teachers’ competence and the learning environment has no influence to the achievement reached by the dean’s listers’. On the other hand, it is still very important to make and to maintain these factors visible in the academic arena for a better learning and for a better outcome. The absence of these factors might affect the performances of the students’ in Caraga State University.

Ethnoecological Studies of Herbaceous Flora of Sahiwal Division, Punjab Pakistan

The present work deals with the ethnoecological studies on the herbaceous flora of Sahiwal Division with special reference to the documentation of ethnobotanical data, phytosociological notes and ethnopharmacological testing of ethnomedicinally important herbs including their antimicrobial, antioxidant and anthelmintic potential. The local people, herbal healer/ hakims were interviewed through questionnaire to collect and document the ethnobotanical data on the local herbaceous plants. In total, 180 herbal plant species, 177 belonging to 43 angiospermic, one species to one gymnospermic and two to pteridophytic families, were found in common use by the local inhabitants as forage / fodder, medicinal, food, feed and various other uses. The crude extracts of five ethnomedicinally important plants were obtained in polar and non-polar solvents (Water, methanol, chloroform and nhexane) by maceration method. The % age yield was found maximum in Cuscuta campestris distilled water extract and minimum in Aerva javanica n-hexane extract. The antimicrobial activity tested by agar well diffusion method indicated strongest antibacterial and antifungal activity by Persicaria glabra against Psedumonas aeruginosa and Aspergillus niger, respectively. The antioxidant activity was found quite convincing when revealed through six different assays. The highest antioxidant activity was shown by Cistanche tubulosa as revealed by ABTS+ Assay, TPC determination, TFC determination and Total antioxidant activity. The highest anthelmintic activity was shown by Cistanche tubulosa while least by Polygonum glabra. Vegetation of the study area was sufficient only for documentation, but insufficient for any economic purpose, yet some plants had shown very important phytosociological attributes. There was no much change in the soil parameters, yet ecological parameters like frequency, distribution and community structure were changing district wise. The TWINSPAN analysis of the vegetation of the study area divided it in to 3 groups and 12 associations, consisting of 159 plant species, in 222 quadrats.