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Population Based Case-Control Asthma Association Studies of Single Nucleotide Polymorphisms in Chromosome 17 Potential Genomic Regions

Thesis Info

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Author

Shahid, Mariam

Program

PhD

Institute

University of the Punjab

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2015

Thesis Completion Status

Completed

Subject

Molecular Biology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12835/1/Mariam%20Shahid_%20Molecular%20Bio_2015_UoPunjab_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726965604

Similar


Single nucleotide polymorphisms (SNPs) on 17q21 are known to be associated with asthma disease in multiple populations. This study was designed to know whether this region is associated with asthma in Lahore region population or not. A total of 200 asthma patients and 100 healthy controls were enrolled from different hospitals of Lahore, Pakistan. Twenty five SNPs from chromosomal region 17q21 were selected for analysis in cases and controls by single base extension method and capillary based genetic analyzers. Associations with asthma were analyzed using basic allelic model, genotypic model and results were adjusted for various cofactors by logistic regression analysis using PLINK v1.9. Pair wise Linkage disequilibrium among the SNPs was analyzed and LD map from healthy controls was constructed using Haploview software. Five of the SNPs (rs3816470, rs1011082, rs6503525 rs11078928 and rs1008723) are suggestive of quite significant association with asthma under genotypic model. Five of the remaining SNPs (rs2290400, rs12603332, rs4065275, rs12603332and rs3894194) showed significant associations in the presence of cofactors like gender, smoke exposure in early years and urban or rural environment. Haplotype “TTGTACC” for rs11078928, rs2290400, rs1008723, rs7219923, rs4065275, rs12603332 and rs4795405, appears to be a significant risk factor for predisposition to asthma. The association of genomic region 17q21 with asthma in Lahori population is reported in this study.
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مولوی نور عظیم ندوی

آہ! مولوی نور عظیم ندوی
دارالعلوم ندوۃ العلماء کے لائق فرزند اور ہونہار استاد مولوی نور عظیم ندوی چند ماہ کی علالت کے بعد وفات پاگئے، اِناﷲ وَ اِنا اِلَیہ رَاجِعُون۔
وہ دارالعلوم سے فراغت کے بعد مزید تعلیم کے لیے مصر گئے، اردو کی طرح عربی لکھنے اور بولنے کی اچھی مشق تھی اور درس و تدریس کے ساتھ ہی تقریر و تحریر میں بھی اپنا جوہر دکھاتے تھے، جلسوں کی نظامت بڑی خوبی اور سلیقہ سے کرتے تھے، جس سمینار کی کاروائی وہ چلاتے وہ ضرور کامیاب ہوتا۔
پڑھنے لکھنے کا اچھا ذوق تھا اور اسی میں ان کا سارا وقت گزرتا، ندوۃ العلماء سے شائع ہونے والے اردو اور عربی جرائد میں ان کے مضامین وقتاً فوقتاً چھتے تھے۔ ایک زمانہ میں ندائے ملت کے عملاً وہی اڈیٹر تھے، تعلیم اور دوسرے موضوعات پر اس کے خاص نمبر بھی نکالے، مولانا سید ابوالحسن علی ندوی مدظلہ کی سرپرستی میں رابطہ ادب اسلامی کا قیام عمل میں آیا تو اس کے روح رواں مولانا سید محمد رابع ندوی کے یہی دست راست اور رابطہ کے ترجمان کے ایڈیٹر بھی تھے۔ ان کے پاس بعض اشخاص اور اکیڈمیوں کے مسودے تبصرے یا اصلاح کے لیے آتے تھے جن کو بڑے غور و توجہ سے پڑھتے، تحریر کی خوبیوں اور خرابیوں پر ان کی نظر فوراً پڑتی۔ اس معاملہ پر مولانا علی میاں مدظلہ بھی ان پر اعتماد کرتے تھے۔
ان کا وطن ضلع بستی تھا اور وہ مسلکاً اہل حدیث تھے لیکن ندوۃ العلماء میں شیرولشکر کی طرح گھل مل گئے تھے، بڑے خاموش طبع، کم سخن، خلیق اور متواضع تھے، ان کی عمر پچاس (۵۰) کی رہی ہوگی، آئندہ ان سے بڑی توقعات وابستہ تھیں لیکن ابھی اپنی چمک دمک بھی نہیں دکھانے پائے تھے کہ وقت موعود آگیا۔
؂ خوش درخشید ولے...

Spirituality and Psychological Well-Being Among Muslims and Christians Adolescents and Young Adults

The present study examining the relationship between spirituality and psychological well-being among Muslims and Christians adolescents and young adults. Daily Spiritual Experience Scale and Warwick Edinburgh Mental Well-being Scale were used to examine the study variables. The present study was carried out on the sample of (N = 254) i.e. Muslims (n = 123) and Christians (n = 131). The sub sample of Muslims and Christians were further divided into males (n = 48) and females (n = 75). Similarly Christian males (n = 60) and Christian females (n = 71). The instruments used to measure the variables possessed satisfactory reliability i.e. Spirituality (α =.80) for Muslims and (α =.92) for Christians and psychological well-being (α =.82) for Muslims and (α =.84) for Christians. Results of the study revealed that spirituality not only had significant positive correlation with psychological well-being but also found to be significant positive predictor of psychological well-being among Muslims as well as Christians. Additional findings of the study further revealed that significant differences exist in the terms of gender and age. Limitations, suggestion and implications were also discussed at the end of the study.

Relationship Between Cognitive Levels, Moral Reasoning and Academic Achievement of Secondary School Students

The purpose of the study is to assess relationship between cognitive levels, moral reasoning and academic achievement of the secondary school students. The objectives of the study were; 1) To find out cognitive development level of the students; 2) To determine moral reasoning level of the students; 3) To measure students’ academic achievement on the moral contents they studied; 4) To analyse comparative effectiveness of English and Urdu medium institutions in terms students’ cognitive development, moral reasoning and academic achievement, and; 4) To explore relationship between cognitive development, moral reasoning and academic achievement of the students. The population of the study was secondary level students studying in the FGBEIs of ICT. The sample of the study was selected from the 20 educational institutions, 10 each from English and Urdu medium institutions. The study addressed 10 research questions. The data were collected on the three variables by using three research instruments. The Science Reasoning Tasks were used to measure cognitive levels, a test based on moral dilemmas was constructed to determine moral reasoning levels and an achievement test based on moral contents was developed to assess moral knowledge levels of the grade 10 students. The given three instruments were administered on 600 respondents. The collected data were analysed by SPSS 15 version. According to the nature of the data and to address the research questions, different statistical techniques i.e. descriptive statistics, t-test, Wilcoxon Signed Rank Test, Spearman rho and Partial Correlation were applied. The major findings of the study are; 1. More than half of the grade 10 students were at Concrete Operations level and vii majority of them were at Good boy-Nice Girl Orientation moral reasoning level while most of the respondents were at outstanding level in moral knowledge. 2. The students studying in English medium institutions are better in cognitive development, moral reasoning and moral knowledge, than those studying in Urdu medium institutions. 3. The negligible relationship between cognitive development levels and moral reasoning of the secondary school students does not allow us to determine the moral reasoning level of the students. 4. The secondary level students’ identified cognitive levels allow us to predict to some extent students’ knowledge level. 5. The small correlation between moral reasoning and moral knowledge indicates that the secondary level students’ moral knowledge least influence their moral reasoning development.