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Home > Possible Role of Neuropeptide Y Npy on Hormones During Different Phases of Menstrual Cycle in Adult Rhesus Monkey

Possible Role of Neuropeptide Y Npy on Hormones During Different Phases of Menstrual Cycle in Adult Rhesus Monkey

Thesis Info

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External Link

Author

Kausar, Nahid

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2013

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/2606/1/2618S.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726972858

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Neuropeptide Y (NPY) acts at the hypothalamus to regulate the reproductive function by stimulating the release of GnRH from hypothalamus. In the present study a group of 5 female adult rhesus monkeys (Macaca mulatta), 5.5-9 years old, mean body weight of 10.31±0.90 kg and with menstrual cycle of 31 days was used. Changes in their body weight, behavior and sex skin color were observed throughout the cycle. Menstrual cycle of each monkey was monitored daily by recording the onset and duration of menstrual bleeding with vaginal swabs. Baseline profile of estradiol (E2), progesterone (P), prolactin (PRL) and growth hormone (GH) were measured by collecting blood sample (2 ml) on different days throughout the menstrual cycle of 31 days. Sequential blood samples (2 ml) were collected at an interval of 15 minutes for one hour before NPY administration for the hormonal baseline and for 2 hours and 15 minutes after NPY administration. In order to study the effect of NPY on plasma E2, P, PRL and GH levels on day 1 (menstrual phase), day 7 (follicular phase), day 15 (peri-ovulatory phase) and day 21 (luteal phase) of menstrual cycle, 200 μg of NPY in single bolus intravenous injection was given. Individual and mean body weight during the menstrual cycle was not significantly different. After NPY administration monkeys were relaxed and comfortable. Sex skin coloration changed progressively from whitish pink to deep red following menstrual to periovulatory phase and then decrease in colour intensity occurred during luteal phase. Baseline profile of estradiol showed that plasma E2 concentration was significantly high (P<0.001) in the periovulatory phase of menstrual cycle compared to menstrual, follicular and luteal phases. The luteal phase plasma E2 level was significantly low compared to follicular phase (P<0.003) but not significantly different from the menstrual phase. Plasma estradiol level 15 minutes after NPY administration increased non-significantly in all the four phases of menstrual cycle compared to baseline at 0 minute. Then, subsequent significant temporal increase till 45 minutes on day 1, 75 minutes on day 15, 60 minutes on day 7 and day 21 followed by subsequent significant temporal decrease. At the end of experiment plasma estradiol attained the basal level in all the four phases. Baseline profile of plasma progesterone showed significantly low levels during menstrual, follicular and periovulatory phases compared to the luteal phase. No significant difference was observed in the plasma P concentration between menstrual, follicular, and ovulatory phases. In all the four phases of menstrual cycle plasma progesterone level 15 minutes after NPY administration increased non-significantly followed by significant temporal increase till 60 minutes on day 1, 105 minutes on day 7, 135 minutes (i.e. till the end of experiment) on day 15 and 30 minutes on day 21. After then non-significant temporal decrease on day 7 and significant on day 1 (P<0.0002) and day 21 (P<0.0007) was observed. The baseline profile of plasma PRL showed that plasma PRL levels were significantly high during menstrual (P<0.013) and periovulatory phases (P<0.023) compared to luteal phase. Plasma prolactin level of follicular phase was non-significantly lower than menstrual and peri-ovulatory phases. The plasma prolactin levels of follicular and luteal phases were not different. In plasma prolactin concentration after 15 minutes of NPY bolus injection a non-significant rise was observed on day 1 followed by non-significant temporal increase till 30 minutes and then significant temporal decrease till the end of experiment. On day 7 non-significant and on day 15 significant increase in plasma prolactin level was observed 15 minutes after NPY administration followed by significant temporal decrease on day 7 (P<0.0005) and day 15 (P<0.009). On day 21 a non-significant decrease in plasma prolactin level after 15 minutes of NPY administration followed by significant temporal decreased till the end of experiment. Regression analysis of variance showed highly significant temporal decrease (P<0.0003). The base line plasma in all the four phases of menstrual cycle GH levels in all the four phases of menstrual cycle were non-significantly different (P>0.05). NPY administration inhibited the plasma GH level in all the four phases of menstrual cycle. On day 1 (menstrual phase) of menstrual cycle plasma growth hormone level 15 minutes after NPY administration decreased non-significantly with subsequent non-significant temporal decrease till 45 minutes followed by significant temporal increase till the end of experiment. A highly significant decrease in plasma GH level was observed on day 7 (follicular phase) and non-significantly on day 15 (periovulatory phase) and day 21 (luteal phase) of menstrual cycle 15 minutes after NPY administration followed by non- significant temporal decrease on day 7 and day 15, but significant temporal decrease on day 21 (P<0.004) till the end of experiment. These results show that NPY has stimulatory and inhibitory effects on the ovarian and pituitary hormones by acting as a modulator, neurotransmitter and neurohormone. NPY has applications in pharmacological fields and can be used for further research.
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شعر و شاعر سبھی کمال ہوئے

شعر و شاعر سبھی کمال ہوئے
لب ہلے اور پل خیال ہوئے

میں نے یزداں سے پوچھنا یہ ہے
کیسے یوسف ہیں خوش جمال ہوئے

تیرے ہونٹوں کا تذکرہ نہ ہوا
ختم سارے سخن خیال ہوئے

ان کی مژگاں کی بات کیا چھیڑوں
ان پہ قربان سو ہلال ہوئے

تیری تصویر بھی ملی نہ کہیں
آج دل کو عجب ملال ہوئے

رنگ، تتلی، کلی، صبا، شبنم
یہ فضاؔ کے ہیں خد و خال ہوئے

IMPLEMENTASI KEBIJAKAN SISTEM ZONASI PADA PENERIMAAN PESERTA DIDIK BARU (PPDB) 2019/2020 DI KOTA YOGYAKARTA

The acceptance of new students (PPDB) using the zoning system was first implemented in Yogyakarta in 2018/2019. This zoning system aims to equalize education and improve the quality of education. This research was conducted to find out how the zoning system policy in Yogyakarta city under the auspices of the Yogyakarta City Education Office. This research uses qualitative approach with case study method. The results of the study showed that the zoning system in Yogyakarta has changed from the previous year. In 2019/2020 the zoning system provides a 10% chance of superior seeds, 30% of school zones, 10% of poor learners, 40% of quality pathways, 5% of out-of-school zoning pathways, and 5% of special pathways such as prospective learners whose parents moved from other regions to Yogyakarta. With this zoning system, the whole community has the opportunity to get a good quality education.

Spatio-Temporal Trends and Integrated Management of Sugarcane Whitefly, Aleurolobus Barodensis Mask. , Aleyrodidae: Homoptera

The experiments were conducted to study the spatio temporal trend and integrated management of whitefly, Aleurolobus barodensis (Mask.) (Aleyrodidae: Homoptera) during 2008 to 2009 in sugarcane growing areas of various districts of the Punjab. The results are abstracted under the following sub headings: Spatio-Temporal Trend of Whitefly Three farmer’s fields surrounding each of the Chinar Sugar Mills, Tandlianwala, Faisalabad; Ashraf Sugar Mills, Bahawalpur and Jamal Din Wali Sugar mills, Raheem Yar Khan were selected. The population of whitefly (nymphs and adults) were recorded at fortnight interval during the crop season 2008. The data were also correlated with the ambient weather factors. The results revealed that population of whitefly (nymphs + pupae) differed significantly among dates of observation, districts and in interaction between dates of observation and districts. The population was appeared during 2 nd fortnight of June in district Faisalabad and Bahawalpur while in district Rahim Yar Khan it was appeared during 1 st fortnight of July. The maximum peak population was recorded on 1 st fortnight of November each in district Faisalabad (7.96/leaf) and Rahim Yar Khan (14.94/leaf) while on 2nd fortnight of October in district Bahawalpur (15.81/leaf). On an average basis, the district Rahim Yar Khan showed maximum population of whitefly (4.61/leaf) while district Faisalabad possessed minimum population (2.48/leaf). All the weather factors viz. Maximum temperature, minimum temperature, average temperature, relative humidity and rainfall showed non-significant correlation with the population of whitefly in all the districts. On an average basis relative humidity had positive and significant (P ≤ 0.05) correlation with the whitefly population. Relative humidity was found to be the most important factor that contributed maximum in population fluctuation of the pest in all the districts as well as on cumulative basis by exerting 42.3%, 43.8%, 35.9% and 18.3% role in population fluctuation of the pest, respectively. Host Plant Resistance Twenty (20) genotypes of sugarcane viz. BF-16, BF-129, BF-162, CMP-13, CO- 132, COJ-84, COJ-1148, CP-43/33, CP-72/2086, CP-77/400, CPF-237, I-62-96, NIA-98, CPF-243, HSF-240, HSF-242, SPF-213, SPF-234, SPF-245 and SPSG-26 sown in the farmer’s Jhang following RCBD with three replications were tested for their resistancesusceptibility against whitefly during 2008 as a preliminary screening experiment. From these studies, three varieties showing comparatively susceptible trend (HSF-242, COJ-84 and SPSG-26), three intermediate (CO-132, NIA-98 and CP-72/2086) and three showing resistant response (CPF-237, CPF-243 and CPM-13) were selected for final screening trial during 2009. The selected varieties of sugarcane were sown under the same ecological conditions following RCBD with three replications. The data on whitefly population were recorded at fortnightly interval at the appearance of the pest. The results revealed that in the preliminary screening trial during 2008, significant variations were found to exist among dates of observation, varieties and interactions between dates of observation and varieties. The variety HSF-242 was found to be comparatively susceptible showing maximum population of whitefly (nymphs + pupae) per leaf (15.48) while CPF-243 appeared to be comparatively resistant with minimum population of whitefly (5.63/leaf). In the final screening trial during 2009, the trend was found to be almost similar as that of observed in the preliminary screening experiment. The variety CPF-243 was found to be relatively resistant showing minimum population of whitefly (4.01/leaf) while HSF-242 appeared as susceptible with maximum population of the pest (11.09/leaf). The descending order of other genotypes based on population density count per leaf are COJ-84 (9.68), SPSG-26 (8.76), CO-132 (7.29), NIA-98 (6.70), CP-72/2086 (6.12), CMP-13 (5.43) and CPF-237 (4.82). The 1 st fortnight of the month of October during 2008 showed maximum abundance of the pest i.e. 22.85/leaf and there was only one peak in the season. Similarly during 2009 the 1 st week of October showed 16.75/leaf population of whitefly followed by 2 nd week of October i.e. 14.40/leaf. Thus it was concluded that the 1 st fortnight of October is the most suitable period for the development of the pest. Relative humidity during 2009 showed positive and significant correlation (P < 0.01) with r-value of 0.754 while maximum temperature on cumulative basis exerted negative and significant correlation with r-values of 0.419 at P < 0.05. The impact of relative humidity during 2008 and during 2009 was maximum on the fluctuation of the pest and showed 10.5 and 47.3% contribution. On cumulative basis minimum temperature was found to be an important factor and contributed maximum role i.e. 16.01% in population fluctuation of the pest.Role of Morpho-physical and chemical Plant Characters towards Resistance against Whitefly on Sugarcane Various morpho-physical (plant height, cane girth, leaf area, hair density, thickness of leaf seed and moisture in leaves) and chemical plant characters ( total minerals. nitrogen, phosphorus, potassium, calcium, magnesium, carbohydrates, fat, ferrous, manganese, copper and zinc) were studied in various selected genotypes of sugarcane and correlated with the population density of whitefly. POL, brix, CCS and fiber contents in the samples of sugar from each selected genotype were also determined. The selected genotypes differed significantly in all plant characters except moisture percentage, total minerals and magnesium in the leaves. Cane girth, leaf area and hair density on leaf sheath resulted negative and significant correlation with the pest population whereas, plant height and thickness of leaf sheath showed positive and significant correlation. Cane girth had negative and significant impact on the pest population fluctuation and contributed maximum role i.e. 35.2 percent. nd plant height were the 2 Leaf area and most important factors and showed 19.2 and 18.9% role in population fluctuation of the pest. All the regression equation was found to be fitted the best. Nitrogen, potassium, magnesium and zinc contents had positive and significant correlation with the pest population while phosphorus showed negative and significant correlation. Nitrogen percentage in the leaves of sugarcane plants showed positive and significant impact with maximum role i.e. 96.3% in population fluctuation of the pest. CCS in sugar samples had positive and significant correlation while fiber percentage showed negative and significant correlation with the pest population. CCS showed positive and significant role with 49.2% impact while fiber contents in the samples of sugar had negative and significant role with 25.8% impact on the population fluctuation of whitefly. Integrated Pest Management Studies Various control methods such as cultural (de-trashing during July and September) biological (release of 2 nd instars larva per plant, chemical (application of imidachloprid 200 SL @ 568 ml/ha and botanical (application of neem seed kernel extract 5% @ 6.17 litre/ha twice in the season on August 26 and September 27, 2009) were applied singly and in their possible combinations for the control of whitefly on a resistant and asusceptible genotypes of sugarcane. There were sixteen treatments in total including control. The experiment was sown in two sets (one for resistant genotype and the other for susceptible) at three localities viz. in the farmer’s field near Shakarganj Sugar Mills, Jhang, Kamalia Sugar Mills Toba Tek Singh and Chinar Sugar Mills Tandlianwala, Faisalabad, following randomized complete block design with three replications. The data on the population of whitefly (pupae + nymphs) were recorded at fortnightly interval from each locality. The results revealed that the population of whitefly in the farmer’s field near Shakarganj Sugar Mills, Jhang and Kamalia Sugar Mills did not reach to a desired level, therefore, no plant protection measure were taken. The results obtained from the farmer’s field near Chinar Sugar Mills, Tandlianwala, Faisalabad revealed significant variation among dates of observation, treatments and between genotypes. The maximum control of whitefly was recorded to be 1.57 per leaf and 3.49 per leaf with maximum population reduction of 72.45 and 65.68% in susceptible (HSF-242) and resistant (CPF-243) genotypes of sugarcane, respectively in those plots where all the control methods were applied in combinations. The release of 2 nd instars larva per leaf of Chrysoperla carnea showed minimum control of whitefly with 4.40 and 8.22 individuals per leaf with minimum reduction in population of whitefly i.e. 22.80 and 19.17% in resistant and susceptible genotypes of sugarcane, respectively. The population of whitefly was higher in susceptible genotype of sugarcane as compared to resistant genotype in all the treatments including control. Cane yield also showed significant difference between genotypes, among treatments and between the interaction of genotypes and treatments. The application of all the control methods in combination resulted in maximum cane yield i.e. 2857.79 and 3061.15 maund/ha in susceptible and resistant genotypes of sugarcane with maximum increase in yield over control i.e. 24.27 and 20.17%, respectively. The release of C. carnea showed discouraging results with minimum cane yield i.e. 2310.27 and 2674.19 maund/ha with minimum increase over control i.e. 0.46 and 4.97% in susceptible and resistant genotypes, respectively. The application of imidachloprid 200 SL @ 568 ml/ha twice in the season when applied alone showed maximum cost benefit ratio i.e. 1: 50.80 and 1: 59.63 in susceptible and resistant genotypes of sugarcane, respectively. De-trashing of older leaves twice in the season showing CBR of 1: 37.09 and 1: 40.98 in susceptible and resistant genotypes ofsugarcane, respectively. The minimum CBRs were recorded to be 1: 8.26 and 1: 8.85 in those treatments where all the control methods were combined together in susceptible and resistant genotypes of sugarcane, respectively" xml:lang="en_US