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Home > Post-Harvets Biochemical and Microbial Evaluation of Indigenous Carps under Different Storage Conditions

Post-Harvets Biochemical and Microbial Evaluation of Indigenous Carps under Different Storage Conditions

Thesis Info

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Author

Dar, Shahbaz Ahmad

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Zoology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/13601/1/Shahbaz_Ahmad_Dar_Zoology_HSR_2018_UAF_03.04.2018.docx

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726974893

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The present research work was planned to assess the post-harvest biochemical and microbial changes in Indigenous carps (Labeo rohita, Cirrhinus mrigala and Catla catla) under various storage conditions including icing, salting and sun drying. The proposed study was completed in two phases. In the first phase, carps were cultured in the ratio 1:2:1 in earthen ponds under semi-intensive polyculture conditions for a period of six months (two treatments with three replicates). Fish were fed with maize gluten as supplementary feed in the 1st treatment (T1) while in the 2nd treatment (T2) urea fertilizer 0.3g N/100g was added. Growth performance was monitored in terms of morphometric characteristics. Water quality parameters were determined on weekly basis. The results of a phase 1st revealed significant differences statistically (p<0.05) in growth parameters in both the treatments. T2 showed better growth performance in terms of specific growth rate over T1 with a maximum of Catla catla 1.22±0.01, Labeo rohita 1.22±0.00 and minimum in Cirrhinus mrigala 1.15±0.01. In the 2nd phase, the cultured fish stored under icing, salting and sun-drying conditions and samples were drawn for biochemical and microbial evaluation at 0, 1, 3, 7, 14 and 30 days of storage and compared in terms of meat quality and nutritive value with the wild fish obtained from local market. The results of phase 2nd also revealed statistically significant difference (p<0.05) among various parameters tested in this study. Post-harvest proximate analysis of Indigenous carps showed that in icing conditions, wild fish showed higher moisture contents 81.61±0.30% as compared to farmed fish 78.82±0.33%. The same trend was observed in salted and sun-dried fish. Maximum crude protein was observed in sun-dried farmed fish 38.07±1.83% and minimum in wild carps stored in icing conditions 14.88±0.11%. Sun-dried farmed fish deposited more fat 7.96±0.36% as compared to sun-dried wild species 7.74±0.35% also wild fish stored in ice showed low crude fat 1.97±0.06% as compared to farmed fish 2.14±0.06%. Maximum ash contents were observed in wild salted fish 15.19±1.11% and farmed salted fish 14.74±1.06% while wild and farmed fish stored in icing showed minimum ash contents 1.72±0.02% and 1.62±0.02%, respectively. The results of the organoleptic quality assessment (OQA) revealed that farmed raised carps showed better OQA at all the three storage conditions; icing 8.49±0.09, salting 8.25±0.11 and sun-drying 7.47±0.17 as compared to wild species; icing 7.50±0.09, salting 7.28±0.11 and sun-drying 6.55±0.16. Sun-dried farmed species showed better protein solubility 72.15±2.19% as compared to iced 68.63±1.61% and salted fish 69.56±1.69%. Similar trends were observed in wild species with a maximum in sun-dried 70.04±2.17% and minimum in iced fish 65.84±1.62%. Total volatile base nitrogen (TVBN) was observed maximum in farmed 16.26±0.83 mg/100g and wild salted fish 17.93±0.88 mg/100g over other storage conditions. pH value was observed minimum in farmed sun-dried 6.70± 0.10 and wild fish 6.83±0.09 while the pH values of farmed and wild iced and salted fish showed a non-significant difference (p<0.05). Total plate count (TPC) was observed highest in iced 5.41±0.04 log10 CFU/g and minimum in salted fish 4.88±0.06 log10 CFU/g while in wild species, sun-dried fish showed maximum TPC value 6.31±0.07 log10 CFU/g.Correlation matrix for overall data of indigenous carps (Farmed + Wild species) showed that moisture contents was negatively correlated with protein, fat, ash, TVBN, pH, TPC and positively correlated with PS. Crude fat was positively correlated with ash, PS, TVBN, TPC and negatively correlated with pH. Ash showed a positive correlation with TVBN, pH and negatively correlated with PS, TPC. PS was negatively correlated with TVBN, pH and TPC. TVBN showed a positive correlation with pH and TPC while pH showed a positive correlation with TPC. In conclusion, traditional techniques of post-harvest (e.g. icing, salting and sun-drying) affected endemic carps meat quality and the fish stored in ice showed better meat and nutritive quality as compared to salted and sun-dried fish.
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76. Al-Insan/The Human Being

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I/We begin by the Blessed Name of Allah

The Immensely Merciful to all, The Infinitely Compassionate to everyone.

76:01
a. Was there not a time in the past when the human being was nothing even to be mentioned?!

76:02
a. Indeed, it is WE WHO created human being of a drop of the mingling of seminal and
ovarian fluid of male and female, so that WE may test him during his lifetime.
b. Then WE enabled him with hearing and seeing and intellect.

76:03
a. And WE guided him on to the Right Path
b. to see whether he be grateful by following the Right Path, or be ungrateful by straying off the Right Path.

76:04
a. WE have definitely prepared for the ungrateful - by being sinful - chains and shackles and
a blazing fire.

76:05
a. As for the grateful - by being righteous - they will drink from cups containing a mixture of camphor/musk -

76:06
a. from a spring at which the servants of Allah will drink,
b. - making it gush out abundantly.

76:07
a. The righteous are the ones who used to fulfill their vows, and
b. stand in awe of the Time the evil of which will be encompassing everyone.

76:08
a. And they - the righteous – used to give food
- to the needy, for the love of HIM,
- and also to the orphan,
- and to the captive/prisoner of war,

700 Surah 76 * Al-Insan

76:09
a. saying within themselves:
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أساليب الحافظ الزيلعي في نقد متون السنة من خلال نصب الراية

The methods of Al-Hafiz Al-Zaili in criticizing of text of the Sunnah as described in his book Nasbu Al-Raya in analyzing the hadiths of Hidayah. The science of criticism is well known science since the era of the Companions, and critics of the hadith of the honorable companions have played a very important role in the field of narration and carefully criticizing it. And by passage of the era of the Companions and beginning the era of the followers (tabieen), the criticism became more obvious depending on the growing need, especially after spreading of lie, and creation of fake hadith, which led the critics to further research and verification, for scrutiny between the narrations and then differentiation between the right and the weak. The imams and scholars of hadith from the era of the Companions till present continuously inheriting the approach of criticizing the narratives in succession of their predecessors, whether criticizing the narrators or the texts. I have seen that it is worthwhile to stand on the efforts and methods of one of the imams in his criticism of the hadiths and I have chosen the effort of Imam Hafiz al-Zaili through his book " Nasbu Al-Raya in analyzing the hadiths of Hidayah" to learn how he was using the standards traded among the scholars of Hadith for textual criticism of Sunnah. As the those denied the hadiths from orientalists and their followers and those who follow their example simply claim that the scholars of hadith did not criticize the Sunnah in true criticism and even if they have criticized the hadiths, their criticism was only concerning the narratives not the text, now it is clear through this article that the scholars of hadith did not leave the side of the text, but they criticized text as they criticized the attribution of the hadiths. They set solid rules, which remain scholarly proven and accurate forever. We will revolve in this article around the following topics: learning about Al Hafez Zaili and the science of criticism, methods of textual criticism according to Hafiz Zaili, by focusing on: Criticism of the hadiths for violating the explicit meaning of the Qur'an, or for contradicting the Sunnah, or for contradicting the explicit consensus, or for risking and exaggerating the promise or the warning of simple action, or lack thereof in books of hadith These are the most important rules sited by al-Hafiz al-Zaili, which he practiced and criticized the hadiths and distinguished them between the correct and the weak.

Drought Induced Morpho-Physiological Changes in Okra and its Mitigation By

Okra is an important vegetable crop known for its high nutritional value. It needs frequent irrigation for good economic yield. Despite the fact that Pakistan has world’s best irrigation system, it is amongst world’s most water stressed countries. Keeping in view the upcoming water shortage in future, a study composed of four different experiments was conducted during summer season for two consecutive years (i.e. 2013 and 2014). In first experiment, to find out critical crop growth stage and to assess harmful effects of drought on okra growth, quality and yield parameters, deficit irrigation (50% less water than normal requirement) was applied at seedling (S), vegetative (V) and reproductive (R) stage, alone as well as in all possible combinations (i.e. S+V, V+R, S+R, S+V+R). While in second and third experiment the foliar application of salicylic acid and ascorbic acid at different growth stages of okra (2 leaf stage, 4 leaf stage, flowering stage and their combinations) was evaluated for drought mitigation. Additionally, in fourth experiment inoculation of okra seed with different doses of azotobacter prior to seed sowing were also tested under drought conditions. Results of study revealed that drought at seedling stage increased plant mortality percentage. However, the survived plants successfully recovered and gave good yield while drought at vegetative and reproductive stage was highly destructive for morphological and reproductive traits, respectively. However, drought at all growth stages (S+V+R) was found most destructive as compared to single stage. Foliar application of 2 mM salicylic acid at 4 leaf + flowering stage improved okra yield, while application of 2 mM ascorbic acid at 2 leaf + 4 leaf + flowering stage showed good results under water stress conditions. After application of ascorbic acid and salicylic acid revealed significant results in case of morphological (plant height, stem diameter, internodal distance, root length, root shoot ratio, number of leaves, number of branches, leaf area and leaf area index), physiological (photosynthetic rate, stomatal conductance, relative leaf water contents and cell membrane permeability), biochemical (N, K, Ca and chlorophyll pigments) and yield traits (pod length, pod weight, number of pods/plant, number of seeds/pod, fruit yield/plant, fruit yield/ha, seed yield/plant and seed yield/ha). Seed inoculation with 1.5 g per 10 g okra seed was found superior in improving all parameters under water stress conditions except number of branches/plant, total dry matter contents, phosphorus concentration, pod diameter, number of seeds/pod and 1000 seed weight.