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Preparation and Evaluation of Legume Based Weaning Foods

Thesis Info

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Author

Bilal, Ahmad

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/8025/1/Ahmed_Bilal_HSR_2017_Food_Technology_UAF_31.10.2017.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726993708

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Protein energy malnutrition (PEM) is the leading nutritional problem worldwide especially in infants. It refers to the macronutrient deficiency resulting from an inadequate intake of protein and energy. Legumes are locally available, cheaper source of protein and can complement cereals to prepare weaning foods to combat PEM. Among the legumes, cowpea and mungbean have good protein and amino acid profile, but may contain certain anti-nutritional components. Roasting was employed to increase the nutritional profile of the product and to reduce anti-nutritional factors in the product. In the present study, roasted cowpea and mungbean flours were analyzed for anti-nutritional factors (hemagglutinin & trypsin inhibitor activities and phytate content) and were mixed with wheat flour to make composite flour. The proximate analysis of mungbean depicted higher moisture and ash contents (5.93 and 4.02%) as compared to cowpea (3.37 and 3.35%), whereas crude protein, fat & fiber contents in cowpea were higher (23.10, 2.05 & 3.57%) than mungbean (22.45, 1.34 & 3.07%), respectively. The mineral profile explicated that potassium, calcium, sodium, iron and zinc were 28.19, 387.51, 61.20, 78.33 and 45.75 mg/100g in cowpea while 10.46, 7.55, 5.31, 1.28 and 2.17 mg/100g in mungbean respectively. After roasting, phytate contents presented decrement in cowpea and mungbean up to 67.03 and 71.12% as compared to raw counterparts, respectively. Likewise, the percent decrease in hemagglutinin-lectin and trypsin inhibitor activities were 68.85 & 78.47% and 79.81 & 77.00% in roasted cowpea & mungbean as compared to raw versions, accordingly. Amongst composite flours, T0 (100% Wheat flour) had highest moisture content 12.22%, T3 (30% cowpea) showed highest protein content 13.14% while crude fat, crude fiber and ash contents were maximum in T9 (15% cowpea & 15% mungbean) as 1.69, 1.12 and 1.29%, correspondingly. Furthermore, maximum nitrogen free extract (NFE) value was observed in T5 (20% mungbean) as 76.32% and highest water absorption & oil absorption capacity was noticed in T3 1.44 mL/g & T8 (10% cowpea & 10% mungbean) 1.16 mL/g, respectively. Additionally, the emulsion capacity was highest in T8 as 75.75% while emulsion stability was maximum in treatment; T2 (20% cowpea) i.e. 53.33%. Highest foaming stability was observed in T8 as 68.67%, whereas highest foaming capacity (44.74%) was reported in T3. In the product development phase, nine weaning foods were prepared using different ratios of cowpea and mungbean. Three most acceptable weaning food prototypes; W2= Roasted cowpea (20%)+wheat flour (30%)+rice flour (13%)+sugar (10%)+vegetable oil (10%)+milk powder (16%)+vitamin-mineral mix (1%), W5= Roasted mungbean (20%)+wheat flour (30%)+rice flour (13%)+sugar (10%)+vegetable oil (10%)+milk powder (16%)+vitamin-mineral mix (1%) and W8= Roasted cowpea & mungbean (10:10%)+wheat flour (30%)+rice flour (13%)+sugar (10%)+vegetable oil (10%)+milk powder (16%)+vitamin-mineral mix (1%) were selected on the basis of sensory response with overall acceptability scored as 7.67, 7.66 and 7.92 and were renamed as W1S, W2S and W3S, respectively however, Wc= soybean (20%)+wheat flour (30%)+rice flour (13%)+sugar (10%)+vegetable oil (10%)+milk powder (16%)+vitamin-mineral mix (1%) was run as control. Amongst the selected weaning foods, W2S had more moisture content (4.58%) while higher crude protein was found in W3S (17.01%). On the other hand, fat, fiber and ash were highest in WC as 11.11, 4.57 and 2.67% respectively. W3S showed higher loose and packed bulk density values as well as reconstitution index, viscosity and gross calorific value. Regarding growth study parameters, protein efficiency ratio (PER), net protein ratio (NPR) and relative net protein ratio (RNPR) were viewed maximum in W3 as 2.65, 3.34 and 53.18 among the prepared treatments and found relatively comparable with Wc (2.84, 6.28 and 100), correspondingly. In nitrogen balance study, true digestibility (TD), biological value (BV) and net protein utilization (NPU) were highest in W3S as 80.31, 85.09 and 82.67% i.e. quite equivalent to Wc values (91.25, 91.84 & 89.37%), respectively. The acceptability appraisal of prepared infant formulations was conducted through short term infant feeding trails with the help of nursing mothers. The acceptability scores were ranged from 3.18 to 4.07 (out of 5) from day 1 to day 15. Conclusively, the selected legume based weaning foods presented comparable characteristics to that of existing soy based equivalents in terms of protein content, energy value and acceptability test as well as its impact on growth & nitrogen balance aspects.
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I/We begin by the Blessed Name of Allah

The Immensely Merciful to all, The Infinitely Compassionate to everyone.

99:01
a. When the earth will be shaken violently in its mighty quaking to its very core,

99:02
a. and when the earth will throw out its burdens of the dead from their graves,

99:03
a. and when the human being will be crying out in panic:
b. ‘What is happening to it?’

99:04
a. At that Time it - the earth - will narrate its news,

99:05
a. for your Rabb - The Lord would have commanded it to do so.

99:06
a. That Time the people will proceed in separate groups,
b. so that they may be shown the results of their worldly deeds and dealings.

99:07
a. So whoever would have done good, even to the weight of a particle, will see it, and be rewarded for it.

99:08
a. And whoever would have done evil, even to the weight of a particle, will see it, and be rewarded for it.

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Comprehensive Study of Trace Elements in Juices and Soft Drinks and Their Impact on Human Health

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Isolated fungal species compared with literature review both in fruit juices and soft drinks and microbial load with Gulf standard (only in fruit juices) as well as these were correlated with physicochemical parameters. Present study shows that order range of concentration for Cr was not permissible above within the standards sets by the organization and also dietary intake It was found that upper limit of Fe in tetra pack and plastic bottle also range was found in sachet pack was not safe within limits but if compared with dietary intake order and concentration range below the standards. It was found that Mean (range) of Zn was below the US-EPA also dietary intake of trace element. Range of concentration and order for Ni (T> B >S) was above within the standards also was not permissible with dietary intake Upper limit of Mn in all packing was not lies within standards except range of Mn in plastic bottle permissible by W.H.O recommendation. Range of Mn in tetra pack and plastic bottle was below within in daily intake level but upper level in sachet pack was high. Mean range of Cu and Co in all packaging was within in all standards was also small within a dietary intake. In the present work range of Pb was measured in all packing was within US-EPA but not safe according to W.H.O recommendation. Upper limit of range of Cd in tetra pack and plastic bottle and range was found in sachet pack was not safe within standards. Mean of heavy metals (ppm) in soft drinks was accessible. In present work it was found that means value of Cr and Ni in both packaging (plastic pack and tin pack) was not permissible and above within standards Mean value of Fe in both packaging was high and not safe within 0.3 ml/L. It was observed that range of Zn (plastic pack and tin pack) was low as compared to standards Mean concentration of Mn, Co and Cd was approximate within standards Mean concentration of Pb in (plastic pack and tin pack) was expectable within US-EPA, but obove within W.H.O. Statistical analysis (ANOVA) Result showed that null hypothesis for Zn and Ni was rejected for fruit juices as well in soft drinks Zn, Cu, and Cd were rejected synthetic chemicals used in the packaging, storage, and processing of food stuffs. This is because most of these substances are not inert and can leach into the foods, harmful to human health over the long period. For identification of fungi in the juices, direct plating technique was applied and the species were recovered in a variety of tetra pack fruit juices and plastic bottle soft drinks. A.flavus, A.niger, Penicillium sp, Rhizopus sp, Mucor saccromyces, Fusarium, A. Fumigate, Monilia, A. Wentii and Candida albicans. In present study genus Aspergillus was most frequent in juices. None fungal contamination was observed in plastic bottle and sachet pack of a variety of fruit juices and tin pack of soft drink Furthermore present work showed that presence of fungi was frequently observed in tetrapacks of fruit juices may be due to permeability of packing by which they are made cartons was more risk than plastic bottle carton are bent and hot filled in vacuum condition this process causes a depression within the carton which may lead to the entry of air and consequently favors mould(fungi) production. For the evaluation of total viable count (TVE) total coliform count (TCC) fecal coliform (FCC) and total staphylococcal count (TSC) Standard cultural techniques were applied. In the present work observed bacterial load in tetra pack and plastic bottle of a variety of juices and soft drinks but none bacterial load was seen in sachet pack of fruit juices and tin pack of soft drinks. Present work observed that Total viable count (TVC) in Apple, Mango, Punch and strawberry juice was below the standard while in orange and grape was found within the standard (TCC.) Total coliform count in Apple, Mango, Orange, Grape, Punch and strawberry juices was above the standard. Fecal coliform count (FCC) in Mango, Grape, Punch and strawberry was above the standard while they were absent in Apple and Orange. Staphylococcal count was found in Apple, Mango, Punch juices was within the standard while in Orange was above the standard, they were absent in grape and strawberry. But in present work presence of coliform, fecal coliform and staphylococci in fruit juices and soft drinks of different packing materials indicate that they were contaminated. Fruits become contaminated with microorganism during preharvest, harvest and post harvest period of time all the way through fecal material harvesting equipment, domestic animals and wild animal human use, transport container, wild and domestic animals, ice or water. Many microorganisms was found as natural contaminants in soft drink, but moderately the minority be able to cultivate inside the acidic and little oxygen atmosphere All assessment it was determined that fruits juices and soft drinks might cause serious hazards to human health. It was concluded that care and caution should be taken to improve the quality of consumer product in every as pact on the basis of may research work for health view do not use ready to eat drinks like juices and soft drinks should be utilize fruits and homemade juices since they are actually favorable for our health and life.