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Home > Prevalence and Management of Meloidogyne Graminicola Golden and Birchfield on Rice Oryza Sativa L.

Prevalence and Management of Meloidogyne Graminicola Golden and Birchfield on Rice Oryza Sativa L.

Thesis Info

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Author

Jabbar, Abdul

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Plant Pathology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/11565/1/Abdul%20Jabbar%20plant%20patholoy%202019%20uaf%20fslbd%20prr.docx

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727004372

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Meloidogyne graminicola is one of the major pest threatening rice production in the world. Studies to estimate M. graminicola prevalence and incidence were conducted during 2014-2016 in rice fields of Faisalabad and Chiniot districts of central Punjab, Pakistan. Studies revealed variation in prevalence and incidence of M. graminicola at both districts. Maximum prevalence of M. graminicola was observed 22.5% and 27.5% in Chiniot and Faisalabad respectively during the rice growing season of 2016, while, 20% from Chiniot and 25% from Faisalabad during 2015 and minimum prevalence was recorded 17.5% and 20% during 2014 respectively. The severity of nematode attack on the rice roots and infested fields was based on different attributes like galling index, root-knot nematodes (RKN) per root system, RKN per 100ml of soil, stylet bearing nematodes and non-stylet bearing nematodes were recorded with varying degree of infestation at various locations. Meloidogyne graminicola isolates were recovered from soil and root samples. The isolates were identified on the basis of perineal pattern and ITS based sequencing. Eleven alternate host plant species were examined in this study that showed varying degree of M. graminicola infestation. ITS sequencing and phylogenetic analysis of eight isolates indicated that most of the isolates from this study clustered together with isolates of India, Nepal, Vietnam and China. The results indicate that rice is severely attacked by M. graminicola, necessitating the development of appropriate control measures for its management in rice and other crops related to gramineae family. Twelve cultivars of rice and forty eight advance lines showed different reaction against M. graminicola i.e. tolerant, partially tolerant, susceptible and highly susceptible. Response of forty eight advance lines and 12 cultivars against root knot nematode M. graminicola were observed. Among twelve cultivars Basmati Pak, Super Basmati, PK-386 and Sheen Basmati showed susceptible reaction while Basmati 385 showed highly susceptible reaction and rest seven varieties were moderately susceptible (PS-2, Kashmir basmati, IR-24) and partial tolerant (Basmati-2000, NIAB IR-9, Basmati-198, Basmati 515 ). Among 48 advance lines ten lines showed tolerant reaction, fourteen lines showed partial tolerant, fifteen advance lines showed moderately susceptible and eight advance lines showed susceptible response. Nematicidal potential of five synthetic chemicals i.e., Furadan Rugby, Match, Polo and Matanza were tested against M. graminicola at their standard (S), half (S/2) and double dose (2S) levels. Results of in vitro study disclosed that all chemicals except Polo significantly inhibited egg hatching and J2s mortality of M. graminicola. Furadan, Rugby, Match and Matanza were found effective to inhibit 88.4, 86.3, 89.7 and 81.0% egg hatching, respectively as compare to control while Polo inhibited only 32% egg hatching. Moreover, Furadan and Rugby significantly inhibited the invasion and development of nematodes on rice compared to control. In field study, all chemicals were applied either as root dip or soil application. Match, Furadan and Matanza treated plants observed significantly lower number of eggs and J2s against control. Both applications methods were effective, but soil application was the best. All the chemicals applied either as root dip or soil application improved plant height, shoot weight, grain weight by inhibiting nematode reproduction i.e., gall formation, development of adult females, eggs and nematode population in soil except Rugby that caused phytotoxic effects on plants. All the chemicals were found more effective at their standard dose than double or half dose. In conclusion, soil application of all tested chemicals in this study except Rugby seemed viable option for chemicall control of M. graminicola in rice fields of Pakistan.
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زلزلہ اور ہم

زلزلہ اور ہم
نحمدہ ونصلی علی رسولہ الکریم امّا بعد فاعوذ بااللہ من الشیطن الرجیم
بسم اللہ الرحمن الرحیم
صدرِذی وقار اور میرے ہم مکتب ساتھیو!
آج مجھے جس موضوع پر لب کشائی کرنی ہے وہ ہے:’’زلزلہ اور ہم‘‘
موضوع بہت طویل ہے اور وقت قلیل ، لیکن مجھے اس مختصر سے وقت میں مذکور ہ عنوان پر اپنے خیالات کا اظہار کرنا ہے۔
معزز سامعین!
زلزلے کے بارے میں مختلف قوموں کے مختلف نظریات ہیں یونانی قوم میں زلزلے کے بارے میں یہ تصور تھا کہ یہ عظیم سمندری اشتعال کا نتیجہ ہوتے ہیں ،میکسیکن ، و یکیورس ، کیلی فورنیا کے قبائل کا عقیدہ تھا کہ ایل ڈیبلو نامی انڈین خدا نے زمین کے ایک بڑے خطے کو کاٹ کر اپنے لئے مختص کر لیا، منگولیا چین کے باسیوں کا عقیدہ تھا کہ بڑے مینڈک نے اپنی پیٹھ پر دنیا کو اُٹھایا ہوا ہے جب وقتاًفوقتاً مینڈک اپنے جسم کو حرکت دیتا ہے تو زلزلے آتے ہیں، سائنس کا نقطہ نظر یہ ہے کہ ہزاروں بلکہ لاکھوں سال سے جاری زمین کے اندر مختلف قسم کی تبدیلیاں ، سمندری چٹانوں کی توڑ پھوڑ فالٹ کی صورت اختیار کر جاتی ہیں زمین کی تین سطحوں میں سے پہلی سطح کرسٹ ، دوسری مینٹل اور تیسری کور کہلاتی ہے۔ مینٹل سیمی لیکوڈ ہوتی ہے، کرسٹ اس پر تیرتی ہے جب کہ کورز مین کا درمیانی حصہ ہوتا ہے جب اندرونی چٹانیں ٹوٹتی ہیں تو ان تبدیلیوں سے پیدا ہونے والے دباؤ کو یہ غیر متوقع انداز میں خارج کرتی ہیں تو اس کی وجہ سے زمین کی اندرونی تہوں میں اضطرابی تخریب پیدا ہو جاتی ہے بعض اوقات کوئی ایک تہہ اس دباؤ کی وجہ سے ٹوٹ بھی جاتی ہے اور زمین کی بالائی سطح پرخارج شدہ توانائی شد ید جھٹکوں کا باعث بنتی...

الوحدة العضوية في القصيدة العربية قديما وحديثا

The issue of harmony and unity in the Arabic criticism and poem is of prime concern, which gained the great importance in modern criticism; as various critiques are of the different opinions regarding its existence, significance, applications and concepts even; in both ancient Arabic criticism and modern poetry. The present study will investigate to find out its roots in the ancient Arabic criticism and poetry; and to prove its references in modern Arabic criticism and poetry while indicating how this issue is kept alive by contemporary poets.

Potential of Microbial Insecticides and Diatomaceous Earth Against Three Coleopterous Insect Pests of Stored Grains under Different Abiotic Conditions

Sitophilus granarius (L.), Rhyzopertha dominica (F.) and Oryzaephilus surinamensis (L.) are the damaging insect pests of cereals and their commodities and are able to cause significant losses. Current research was performed to evaluate the lethal and progeny inhibition effect of microbial-based insecticides i.e entomopathogenic fungi (EPF) Metarhizium anisopliae and Beauveria bassiana as well as bacterial based insecticides (spinetoram and abamectin) solitary andwith combination of two formulations of diatomaceous-earth (DE) against S. granarius, O. surinamensis and R. dominica. Three concentrations (1x108, 1.5x108 and 2x108 spores/kg grain) of each EPF were used while for bacterial based insecticides concentrations were 0.5, 1.0 and 1.5ppm. Similarly, for diatomaceous earth, three different doses (200, 400 and 800 ppm) of every formulation were applied. Completely Randomized Design (CRD) was used with three repetitions of all treatments. Mortality of test insects was recorded after 7, 14 and 21 days while after 60 days for post-treatment progeny build up. Each bioassay was performed at three levels of temperature (25°, 30° and 35°C) and relative humidity (r.h.; 45, 60 and 75%). Collected statistics were examined with applicable statistical methods using R-Software.The data analyses had shown diversified results regarding test insect susceptibility at different abiotic conditions. In case of Bacterial based insecticides, the response of treatment remained best at high temperature and low r.h. for all test insects. Complete control was achieved after 14 days for S. granarius and R. dominica while for O. surinamensis 100% mortality was not attained even after 21-d of exposure period. Progeny production was remained totally suppressed for S. granarius and R. dominica while in case of O. surinamensis there was significant reproduction at some abiotic conditions with maximum production at temperature of 30°C with 75% r.h. The response of-D.E against test insects was also effective and the most vulnerable species was O. surinamensis followed by S. granarius and R. dominica. It was observed that high temperature, low r.h. levels, higher dosages and long exposure periods increased the efficacy of DEs. Regarding progeny production, low temperature and high humidity remained most favourable for progeny emergence in DE-treated grains. The effects of EPF remained somewhat similar to the response of DE but overall mortality was low. Among tested species of insects R. dominica remained most susceptible to the application of EPF followed by O. surinamensis and S. granarius. The best abiotic condition for B. bassiana was low temperature (25°C) and moderate (60%) humidity while for M. anisopliae moderate temperature (30°C) and moderate (60%) humidity remained suitable at which maximum response was attained. Long exposure intervals and higher dose rates increased the mortality in each case. In the case of progeny development, the emergence of offspring was suppressed at moderate and low temperature for M. anisopliae and B. bassiana respectively with moderate r.h. When DE was applied in combination with the microbial insecticides, a synergistic effect was noticed in all combinations and R. dominica and S. granarius remained more susceptible as compared to O. surinamensis. The response of abiotic condition was significant for combination of DE and EPF while for DE and bacterial based insecticide it was non-significant. The results of the study divulge that all applied microbial based insecticides and DE are very effective for the control of these test insects and different abiotic conditions are responsible for affecting their efficacy. Furthermore, the combinations of these microbial insecticides with DE have a synergistic response against test insects. This study also recommends that attention should be paid to the interaction of abiotic factors with the efficacy of DE and microbial insecticides before planning IPM strategy for stored grain insect pests.