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Home > Production and Characterization of Biochar from Organic Waste and its Application for Bioremediation in Diesel Contaminated Soils

Production and Characterization of Biochar from Organic Waste and its Application for Bioremediation in Diesel Contaminated Soils

Thesis Info

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Author

Sadia Aziz

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Microbiology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/11951/1/Sadia%20Aziz%20Microbiology%202019%20qau%20isb%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727019494

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The pollution of the soil and water due to accidental/anthropogenic release of complex hydrocarbons has been a serious environmental problem all over the world.Current study focused on preparation of biochar from fruit and vegetable waste and sewage sludge and its application for bioremediation of diesel polluted soil. Biochar prepared was physio chemically characterized by pH, CEC, FTIR, SEM, EDX, XRD, surface area and pore analysis, proximate analysis, elemental analysis, nutrient analysis and thermogravimetric analysis. Study was conducted at pilot scale. Both types of biochar was applied in diesel polluted soil in a pot experiment in combination with cow dung to evaluate their relative efficacy in bioremediation. Two set ups were run in parallel. In one set up amendments were applied on naturally contaminated soils while in second setup artificially contaminated soil was used. The diesel degradation was estimated by standard curve, FTIR and Gas chromatography.Physicochemical parameters like pH, EC, total carbon, total nitrogen, phosphorous, potassium in soil were analyzed during treatment.Microbiological analysis was performed by CFU count and dehydrogenase activity. Soil microbial diversity was analyzed by Illumina 16S RNA sequencing. Analytical characterization of biochar depicted its crystalline nature with presence of carbon nanotubes and circular pores. Both types of biochar were aromatic, nutrient rich and thermostable materials. Higher percentage yield and ash content was obtained for sludge biochar while high fraction of fixed carbon was recorded in fruit/vegetable waste biochar. Fruit/vegetable waste biochar exhibited larger BET surface area of 52.50m2/g as compared to sludge biochar having surface area of 46.85m2/g. Results of diesel bioremediation studies showed that soil amended with biochar not only increased the soil pH, EC, nutrients and carbon content but also promoted the degradation of hydrocarbons. In Set up 1 diesel oil degradation efficiency recorded was up to 72.27±0.50% for fruit/vegetable waste biochar and 75.63±0.351% for sludge biochar. Highest removal efficiency was for SDN treatment with 82.86±0.60% following VDN treatment with removal capacity of 78.51±0.38%. In Set up 2 having artificially contaminated soil removal efficiency was recorded higher in both VDA and SDA with 76.23±0.42% and 73.24±0.21% while in treatments VA and SA it was 65.28±0.44% and 68.89±0.63% respectively. Highest microbial count and dehydrogenase activity was recorded in treatments having sludge biochar along with cow dung in both Set up 1 and in Set up 2. Microbial study of SDN treatment showing highest bioremediation efficiency indicated the presence of 27 different phyla with predominance of Proteobacteria and Actinobacteria contributing 29% and 19% to total bacterial community. Biochar amendment into soil to degrade hydrocarbons is very important consideration for research purposes and land applications as well. Another study was conducted with application of biochar for soil fertility and crop productivity purposes. In modern agriculture practices, application of biochar for improving soil fertility, plant growth and agriculture output is gaining great deal of attention. Both fruit/vegetable waste biochar and sludge biochar were applied separately and in combination to analyze their effect on wheat growth. Results proved that wheat growth and biomass production was highest at 0.5% concentration of biochar mixture while highest microbial count was observed with 1% sludge biochar. Promising wheat growth and shift in relative abundance of microbial community could be resulted from improvement in soil parameters such EC, total nitrogen and carbon along with phosphorous and potassium content. Our results conclude that the biochar amendment in soil at optimum level improve soil properties and stimulate soil microflora which in turn improves the agricultural performance of soil.
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ناصر کاظمی

ناصر کاظمی
ناصر کاظمی کا اصل نام سید ناصر رضا کاظمی تھا۔ ان کی ولادت 8 دسمبر 1925ء جبکہ وفات 2 مارچ 1972ء کو ہوئی۔گئے دنوں کا سراغ لیکر کدھر سے آیا، کدھر گیا وہ، عجیب مانوس اجنبی تھا مجھے تو حیران کرگیا وہ ،جیسے ہزاروں اشعار کے خالق اردوغزل کے رجحان ساز شاعر (۵۲۹۱ - ۲۷۹۱) ناصر رضا کاظمی انبالہ (پنجاب) میں پیدا ہوئے ان کا پورا نام سید ناصر رضا کاظمی تھا۔ ابتدائی تعلیم انبالہ میں ہوئی۔ اس کے بعد اسلامیہ کالج لاہور میں زیر تعلیم رہے۔ اعلیٰ تعلیم کی تکمیل نہ ہو سکی۔۔ ۱۹۴۷ میں تقسیم ہند کے بعد لاہور میں سکونت اختیار کی۔ ناصر کاظمی نے 1939ء میں صرف سولہ سال کی عمرمیں لاہور ریڈیو کے ساتھ بطور اسکرپٹ رائٹر منسلک ہو ئے اور پھر آخری وقت تک کسی نہ کسی صورت ریڈیوپاکستان کے ساتھ منسلک رہے۔اسکے علاوہ وہ مختلف ادبی جریدوں، اوراق نو، ہمایوں اور خیال کے مدیر بھی رہے۔شعر گوئی کا آغاز جوانی میں ہو گیا تھا۔ شاعری کے علاوہ موسیقی سے بھی گہری دلچسپی تھی۔
ناصر کاظمی نے اپنی تخلیقی زندگی کی ابتداء تیرہ برس کی عمر میں کی اور ابتداء میں اس زمانے کے معروف شاعر اخترشیرانی سے متاثرہوکر رومانوی نظمیں اور پھر غزلیں کہیں۔اور میر تقی میر کو اپنا مرشد سمجھا یہی وجہ ہے کہ شاعری میں میر تقی میر کی شاعری کا عکس نظر آتا ہے۔
قیام پاکستان کے بعد ان کے اشعار کی مہک گوشے گوشے میں پھیل گئی اور پاکستان کو ناصر کاظمی کے روپ میں ایک نیا شاعر نصیب ہوا۔ ناصر کاظمی نے پیروی میر کرتے ہوئے اپنے سادہ اسلوب ،قادرالکلامی ،ندرت خیال، سچے جذبوں اور احساسات کی بدولت اردو غزل کا دامن ایسے ایسے تابدار موتیوں سے بھر دیا ہے کہ اردو ادب کی تاریخ میں امر ہو گئے۔کپڑے بدل کر جاؤں کہاں،...

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