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Home > Production and Immunological Evaluation of Polyvalent Vaccine of Avian Influenza Aiv-H9 and Fowl Cholera Pasteurella Multocida

Production and Immunological Evaluation of Polyvalent Vaccine of Avian Influenza Aiv-H9 and Fowl Cholera Pasteurella Multocida

Thesis Info

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External Link

Author

Rafique, Maria

Program

PhD

Institute

Lahore College for Women University

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Biotechnology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/14475/1/Maria%20Rafique%20biotechnology%202019%20lcwu%20lhr%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727021930

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Infectious diseases particularly fowl cholera (FC) and avian influenza (AI) are challenge for birds, causes severe economic loss and hampers to the development of poultry industry in the developing countries including Pakistan. Vaccination programs using monovalent vaccines against the diseases are effective but the repeated vaccination induces stress to the animals and increase cost of vaccination for the farmer. Owing to significant role of hemagglutinin (HA) and neuraminidase (NA) gene in antigenic drift, AIV field isolates leads to escape the immunity provided by vaccines. In this study, the genetic and antigenic nature was explored in currently prevailing H9N2 viruses in Pakistan during year 2017. The efficacy of bivalent AI+FC vaccine was compared to monovalent AI and FC vaccines. H9N2 AIV samples were collected from affected poultry flocks from different areas of the Punjab and were serologically and molecularly confirmed. Positive isolates were propagated on chicken fibroblast cell line. Direct sequencing of HA and NA gene was performed and phylogenetic relationship of identified strains was evaluated. The distribution of mutations was analyzed on different epitopes of HA antigen. To check the antigenic effect of potential predicted substitutions at different positions of HA surface glycoprotein, protein-peptide modelling was done by GlaxayWEB. Previously isolated and characterized AIV subtype “H9N2” and Pasteurella multocida were used as antigen in the vaccine production. Formalin inactivated alum and oil adjuvated AI, FC and AI+FC vaccines were prepared and injected in experimental poultry birds at 0 and 14th day. The serum samples were collected on 0, 14, 21, 48 and 60 days post-priming. Antibody titer was measured by Hemagglutinin Inhibition (HI) for AI “H9N2” virus and enzyme-linked immunosorbent assay (ELISA) was performed for lipopolysaccharide (LPS) of P. multocida. The results showed that H9N2 virus had 99% homology with the earlier reported AIV lineages of Iran, India, Saudi Arabia and Pakistan while their phylogenetic examination showed that the strains of this study belonged to the Pakistani H9N2 viruses isolated during years 2010-2015. The amino acid analysis of isolates (H9N2) showed substitution of A to T at position 198 of HA receptor-binding motif. The in-silico predictive changes in HA’s antigenic sites R180A, T163A and N201A changed the 3D-structure of protein-peptide binding site. The birds exhibited the classical pattern of immune response in multivalent formulation (both oil and alum based) with achievement of peak antibody titers (HI titer 256 and 128; ELISA titer 2.752 and 1.805) at 48th day post vaccination. The HI and ELISA titers in combined vaccine were significantly higher (P < 0.05) than corresponding monovalent formulation. Oil based combined vaccine showed better immune response due to prolonged immunity. In conclusion, our findings demonstrates that present H9N2 AI isolates circulating in poultry flocks were related to previous strains of H9N2 in Pakistan. The change of Amino acid at antigenic sites 163, 180 and 201 play a vital role in determination of the antigen binding to antibody, which may lead to escape immunity provided by vaccine. From immunological point of view, AI vaccine can be combined with FC vaccine and gave better immune response than alone one. Being gram negative, the LPS of P. multocida may acts as a factor contributing towards relatively higher antibody titer in comparison with the respective monovalent vaccine. This combined vaccine may also be helpful to reduce burden as repeated vaccination itself is a stress for poultry birds. It would also be helpful for poultry breeders in reducing the cost as well as frequency of vaccination, leading to less stress and higher immune response in birds. Moreover, oil based combined vaccine is better choice for prolonged titer than alum adjuvanted combined vaccine.
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دیا نرائن نگم

دیا نرائن نگم ، بی ۔ اے
اردو زبان کے مشہور پرانے رسالہ زمانہ کے اڈیٹر دیانرائن نگم نے اسی مہینہ وفات پائی، کالج سے نکلنے کے ساتھ انہوں نے بریلی میں زمانہ کو جو اردو کا ایک معمولی رسالہ تھا، اپنی ادارت میں لیا اور اس کو کانپور لائے اور اس حد تک چمکایا کہ اردو کے رسالوں میں گناجانے لگا، بلکہ اس وقت وہ اردو کا سب سے پرانا رسالہ ہے، پریم چند آنجہانی کو وہی سب سے پہلے اسٹیج پر لائے، ان کے علاوہ اور بہت سے اچھے لکھنے والے اور کہنے والے ہندو اور مسلمان نوجوانوں نے ان کے سایۂ قلم میں تربیت پائی اور کہنا چاہئے کہ زمانہ صرف انہی کی بدولت ہندو اور مسلمان اہل قلم کا سنگم اب تک رہا اور اس کو دیکھ کر تسکین ہوتی تھی کہ ہندو مسلمانوں کی پرانی تہذیب کے شیدائی ہندو ابھی تک زندہ ہیں۔
مدت سے جسے دورِ زماں میٹ رہا ہے
امید ہے کہ زمانہ آئندہ بھی اپنے بانی کی یادگار میں اس کی بنائی ہوئی روش پر چلتا رہے گا۔ تاکہ اس اختلاف آباد ہند کی اس آندھی میں دیانرائن کا یہ دیا جلتا رہے۔
(سید سلیمان ندوی، دسمبر ۱۹۴۲ء)

 

الامام ضیاء المقدسی و منھجہ فی کتابہ الاحادیث المختارۃ

Different scholars have compiled the books which contain a large numbers of authentic Ahadith (Ahadith Sahiha), to achieve this purpose, they introduced different hadith sciences to distinguish between the true and the fabricated hadith. The authentic Sunnah is contained within the vast body of Hadith literature. One of them is Imam Zia ul Maqdasi. Imam Zia Uddin Muhammad bin Abdul Wahid Maqdasi’s book “Al Ahadith al Makhtara” is one of the best books of its kind. Many Islamic scholars have declared it better than Imam Hakim’s book Al Mustadrak. Allama Iraqi, one of his contemporaries said that the Ahadith given in his book Al Ahadith al Makhtara were not ascertained to be authentic before. Only those Ahadith have been given in this book whose asaneed are correct but they have not been reported by Imam Bukhari and Imam Muslim. Also, one of the strengths of this book is that it reflects the glimpses of Muajam. Imam Maqdasi wrote this book in the manner of Masaneed that is to say that he mentioned the name of the companion of the Holy Prophet (SAW) and then reported his traditions. Sometimes he also indicates the factors responsible for the interruption in the authenticity of Ahadith. But, sadly, Imam Maqdasi passed away and could not complete this great book. In this article I will discuss the Imam Zia ul Maqdasi approach towards “Ahadith Sahiha” in his book Al Ahadith ul Mukhtara.

Energy Efficient Radio Resource Management in Wireless Networks

Energy Efficient Radio Resource Management in Wireless Networks The contemporary extension of telecommunication industry and the hike towards more and more data rates have a profound impact on the power disbursement. More sensitive of the disadvantages associated with this power budget are the climatic perils along with the economic bounds. Consequently, the concept of Energy Efficiency (EE) for attaining green communication is evolved that may help to address the above affairs in current and future wireless networks. Enormous efforts have been done in this regard for the attainment of EE that include efficient power control, channel allocation, interference management and data rate maximization, etc. In addition, the incorporation of new techniques like Device to Device (D2D) and Cognitive Radio (CR) in existing wireless communication will increase the EE of the wireless systems. In this thesis, the notion of EE has been thoroughly investigated, with the basic as well as advanced approaches for its attainment. Therefore, resource allocation problems for maximization of EE have been focused in different wireless frameworks like Single Carrier Frequency Division Multiple Access (SC-FDMA), D2D and CR networks. Resource allocation is the field of wireless communication in which the wireless resources like bandwidth, data rates, transmission power etc., are cleverly distributed among users. Therefore, I have considered channel allocation, transmission power allocation, user admission and mode selection in cellular communication frameworks for the achievement of my goal of increasing the EE. Firstly, I have considered resource allocation problem in uplink SCFDMA for maximization of EE. The joint power and subchannel allocation problem is formulated to maximize the EE metric under constraints on the minimum achieved data rate of each user, the maximum transmit power budget of each user, and the exclusive as well as the consecutive allocation of sub-channels among the users. This problem is a nonlinear and combinatorial optimization problem, which has been transformed to equivalent binary integer programming problem for obtaining the optimal solution. Also, a heuristic suboptimal solution with less computational complexity has been presented. This EE utilization of resources may further be extended to advanced wireless techniques like D2D and CR to complement our goal. D2D is one of the promising technologies that can be used in association with cellular networks for the provision of high data rates, which is the foremost demand of today''s cellular users. These high data rates can further be increased through efficient management of resources, which may also increase the EE of such systems. Therefore, I have also address the mode selection, user admission control and the resource allocation in a pooled D2D and cellular network optimization model, for energy efficient resource allocation. The problem formulated is a Mixed Integer Nonlinear Problem (MINLP), whereas such problems belong to a class of problems whose computational complexity is NP-hard. Finding the optimal solution of such hard problems by the brute force and searching the feasible space is also challenging since the complexity increases exponentially by increasing the admitted users. Therefore, a Mesh Adaptive Direct Search (MADS) algorithm has been proposed to find the EE resource allocation with reasonable computational complexity. The D2D connectivity is nonetheless a game changing technique while considering the high data rates, however, more smart utilization of resources in CR assisted D2D networks, may result in more data rates and EE. Therefore, the problem of maximizing EE of CR assisted D2D networks has been considered, subject to compliance of transmit powers of cellular users and interference constraints of primary users of broadcast network. CR network users are cellular users comprising of cellular and D2D users. Cellular users can opt any of the cellular or D2D mode. The cognitive radio network users opportunistically utilize spectrum of Television (TV) white spaces. The problem thus formulated is NP-complete. MADS algorithm has been used to find near optimal solution. The results of MADS have been compared with the exhaustive search algorithm, which clearly proves MADS to be an ideal algorithm, and can be a choice for real life large cellular networks, due to its low computational complexity.