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Home > Production of Ethanol from Molasses Using Thermotolerant Kluyveromyces Marxianus

Production of Ethanol from Molasses Using Thermotolerant Kluyveromyces Marxianus

Thesis Info

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Author

Shaheen Aziz

Program

PhD

Institute

Mehran University of Engineering and Technology

City

Jamshoro

Province

Sindh

Country

Pakistan

Thesis Completing Year

2010

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1816

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727026014

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This study shows that strain of thermotolerant Kluyveromyces marxianus was used for the production of ethanol and invertase (fructofuranosiadse, Ffase). This strain (D-67283) was collected from Shakkar Gunj Sugar Mills, Jhang, selected through Gamma rays on 1.5 % (w/v) deoxy-Dglucose (DG) in liquid medium after growth at 60 °C for 5 days and designated as K. marxianus M15. The selected mutant strain produced maximum ethanol and Ffase at 48 h of cultivation on different substrates including glucose, sucrose, and molasses each at 10, 12, 15 and 17% total sugars in 23 L fermentor (working volume 15 L). Optimized studies on different carbon sources displayed that product formation rate (Qp) was greater on glucose- followed by molasses- medium but was found to be lower on sucrose medium. Product yield (Yp/s) and specific product yield (Yp/x) were also significantly higher on glucose (15%) whereas found as lower on sucrose medium. Specific product formation rate (qp) was also recorded higher on glucose medium and remained lower on sucrose medium. Nitrogen sources like ammonium sulphate, corn steep liquor (CSL), and urea were added to the growth medium to enhance growth and ethanol formation. All these sources were used at the rate to contain 0.11, 0.16 and 0.21 % nitrogen in the growth medium. The best results were observed for fermentation kinetic parameters of growth and product formation by ammonium sulphate using 0.75% (w/v). In further studies, temperature of fermentation was optimized for maximum ethanol production, and substrate utilization, for both wild and mutant strains of K. marxianus. For this purpose, they were grown at different temperatures ranging from 20- 65 oC. The study further revealed that maximum ethanol production on molasses medium supplemented with ammonium sulphate was used 0.75% at pH 5.5 after 48 h at 40 oC. xx Effect of various agitation rates from 250-450 rpm on production of ethanol by K. marxianus cells was carried out and the maximum amount of ethanol produced on the sugar based used was more than 89.96 %. Further increase in agitation intensity did not increase ethanol production in both organisms. Hence, agitation rate of 300 rpm was optimized. The production of ethanol is an anaerobic fermentation process; therefore supply of oxygen to the yeast culture is of great importance as it is needed to support an initial amount of cell mass for maximum ethanol production. At a constant rate of oxygen supply, agitation rate supports uniform distribution of cells and maintain a constant temperature by uniformly stirring the media to dissipate excess heat. Effect of supplying air to the fermentor at different aeration rates (0.25 - 1.5 LL-1min-1) on ethanol fermentation, amount of ethanol produced was 72.5-74.8 gL-1 when the aeration rate was kept at 1.0 LL-1min-1 for 8 h and then between 0.25 - 0.50 LL-1min-1. Further increase in aeration rate, resulted in lower production of ethanol and greater amount of cell mass in the fermented broth but up to a certain extent. The maximum amount of ethanol (75 gL-1) produced when the aeration rate was kept at 0.30 LL -1min-1. Maximum ethanol specific or volumetric productivity increased with the increased temperature up to 40 oC and 45 °C in the case of wild and mutant respectively. The activation enthalpy for ethanol formation (DH* = 55.6 KJ/mol) pathway was lower than that for phytase production ((DH*70-80 KJ mol-1).The estimated values of enthalpy of Ffase formation (13.1 kJ mo-1l) network was lower as compared with the product inactivation (12.0 kJ mol-1 ) network. This usually happens in the case of thermotolerant organisms as reported earlier. Over all these studies revealed that mutant strain acquired significantly better changes in the genetic make up and qualifies for its evaluation at industrial scale ethanol production.
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فصل سوم: آیاتِ استفہام میں فہمِ عبادات

دین کی اصل توحید ہے قرآن کے مطلوب انسان کی زندگی ایک اللہ کی وفادار ہوتی ہے اس کا ہر سجدہ ،ہر امید، ہر اندیشہ، ہر محبت، ہر خوف، ہر دعا ،ہر عبادت صرف اور صرف اللہ کے لئے ہوتی ہے وہ اپنے رب کے ساتھ کسی کو شریک کرنے کا تصور بھی نہیں کر سکتا اللہ کے سوا کوئی رب نہیں ہے وہ واحد و یکتا ہے وہی معبود برحق ہے ۔

اللہ تبارک وتعالیٰ قرآن مجید میں ارشاد فرماتے ہیں:

" اِنَّ اِلٰهَكُم لَوَاحِدٌ "۔[[1]]

"بیشک الہ تم سب کا ایک ہے"۔

قرآن میں بہت سے مقامات پر ہمیں توحید کا بیان ملتا ہے۔اس آیت میں الہ کی بنیادی نوعیت یہ ہے کہ معبود حقیقی سب کا ایک ہی ہے۔ اس میں تعدد کا احتمال نہیں ہے یہ خدائی اور معبودی ہے نہ اس کے سوا کسی کو معبود بنا سکتے ہیں اور نہ ہی کسی اور سے خیر کی توقع کی جا سکتی ہے۔ یہ توحید ہی سب سے پہلی اور سب سے بڑی چیز ہے جو ملت ابراہیم علیہ السلام کی وراثت کی حیثیت سے اس امت مسلمہ کی طرف منتقل ہوئی ہے ۔ اللہ تعالیٰ اپنی ذات و صفات میں یکتاو یگانہ ہے کوئی اس کی برابری کرنے والا نہیں ہے کوئی اسکا ہمسر مسلمان ہونے کے لیے ان تمام عقائد پر ایمان لانا ضروری ہے جن کو ہمیں قرآن میں حکم دیا گیا ہے۔

 ارکان"رکن" کی جمع ہے اور رکن کسی بھی چیز کا اہم جز ہوتا ہے جس کے بغیر وہ مکمل نہیں ہوسکتی ایمان کے چھ ارکان ہیں لہذا اگر ایمان کا ایک رکن بھی ساقط ہو جائے تو انسان مومن نہیں رہتا خواہ وہ لاکھ ایمان کے دعوے کرتا رہے جیسے...

ڈاکٹر فاروق احمدخان کی تفسیری خدمات اور آپ کے تفردات کا تجزیاتی مطالعہ An Analytical Study of Dr. Farooq Ahmed's Tafsir Services and His Distinctions

For the guidance of humanity, God revealed the Holy Quran. It is the only book which is recited the most. Those who read and teach this book have been called the best people. From the blessed life of the Holy Prophet (ﷺ) till today, scholars have tried their best to make common sense for the less educated people. And Muhadithin have to bind chapters of "Kitab al-Tafseer and Chapters of Commentary" in their own books. Companions and many commentators interpreted. Some of the interpretations were translated into different languages ​​so that common people can benefit from it. Scholars of the Indian sub-continent also interpreted the Holy Quran for the understanding of common people. A link in the same series is Dr. Muhammad Faruk Khan's "Asan Tarin Tarjuma Wa Tafsir" which is written in simple Urdu language. Dr. Sahib's distinctions on special topics make the reader ponder.  In this article, Dr. Farooq Ahmad Khan's services regarding Tafsir and his distinctions about (Alphabets, prohibited trees, forgetfulness of Adam, etc.) will be examined. Along with the opinions of different commentators will also be present. Keywords:               Qura’n, Dr. Farooq Ahmad, Asan Tarin Tarjuma wa Tafsir, distinctions, Commentators.

Effect of Organic Manures on the Yield Components and Yield of Wheat Triticum Aestivum L. under Arid Conditions

Crop yields are reduced due to limited water availability, poor soil fertility and low organic matter contents. This study comprised of four field experiments on the use of organic amendments for growing wheat cv. Bhakkar 2002 to find out their impact on crop growth, yield, nutrients contents and soil characteristics. First experiment compared the efficiency of various organic manures applied singly and in combination treatments on pre- green manured soil. The organic sources used in the experiment were: farmyard manure (FYM), poultry litter (PL), press mud (PM) and sewage sludge (SS) @ 20 t ha –1 . Two years’ data (2004-05 and 2005-06) on crop growth and yield attributes revealed that PL alone performed the best, and FYM alone gave lowest results. Combined application of PL with other organic manures gave lower growth and yield of wheat, while integration of FYM with other organic materials improved its effectiveness. Soil bulk density was not affected significantly by any treatment of organic manures. Contrastingly, FYM and PM alone or combined gave lower N contents in soil; whereas, soil P contents were greater with SS, PL alone and their integration with other organic amendments. Soil and plant K contents were higher under PL, PM and their integration with other organic amendments, while SS gave the lowest results. The highest N and P contents in wheat grain and straw were found with SS, PL alone and their integration with other organic amendments. Generally, PL alone and combined with SS performed better than all other treatments for wheat production. In Experiment-II residual effects of various organic manures treatments used in Experiment-I were evaluated for two seasons (2005-06 and 2006-07). Crop growth and yield parameters were higher with PL+PM and FYM+PM; while, FYM performed the least. Application of PL, SS alone and combined together enhanced N and P contents in soil and plants; while PM / FYM lowered them. Potassium contents in soil and plants were higher under PL, PM and their integration, while SS gave 1 the lowest contents. Residual effect of PL alone or combined with other organic manures especially SS and FYM was greater than all other treatments for wheat. Combined application of PL and FYM with other organic manures improved their effectiveness. In Experiment-III, efficiency of organic manures used as in Experiment-1 was compared on wheat straw pre-manured soil in 2005-06 and 2006-07. Data on various growth attributes of crop indicated that sole use of all the organic manures (FYM, PL, PM, SS) performed better than their combined application, and PL alone out-performed over others. However, yield and yield contributing parameters responded better to the integration of these organic amendments; among them PL+SS and PL+PM gave better results. Use of PL+PM and PM+SS lowered the soil bulk density significantly and to the maximum. Application of PL, SS both alone and combined increased N and P contents in soil and plants over others. Soil and plant K contents were highest under PL, PM alone and their integration, while SS and its combinations gave the lowest K contents. On the overall, PL used alone or in combination with other organic manures especially SS was superior to all other treatments. Fourth experiment compared the efficiency of NPK fertilizers, PL alone and other organic manures (FYM, PM, SS) used in combination with PL and with each other under different available soil moisture depletion levels (ASDLs) on wheat straw pre-manured soil during 2005-06 and 2006-07). Interactions of ASDLs and organic manures showed that PL alone and NPK fertilizer under ASDL 2 (65%) followed by PL+PM+SS gave higher growth measurements and production of wheat. It was concluded that PL whether used alone or integrated with other organic manures is the best for growing wheat in green manured as well as straw incorporated soil. Further, PL should be used at 65% ASMDL for having higher wheat production under rain-fed conditions.