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Home > Production, Purification and Characterization of Biosurfactants from Bacillus Spp. and Their Potential Applications in Food and Pharmaceutical Products

Production, Purification and Characterization of Biosurfactants from Bacillus Spp. and Their Potential Applications in Food and Pharmaceutical Products

Thesis Info

Access Option

External Link

Author

Basit, Madiha

Program

PhD

Institute

Government College University

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Microbiology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/13739/1/Madiha%20Basit_Microbio_GCU%28F%29.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727031054

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Biosurfactants are the secondary metabolites produced by bacteria, fungi and yeast. The aim of the present study was to isolate the Bacillus spp. from the soil to carry out production, extraction and purification of biosurfactants and to evaluate different properties for their potential applications in food and pharmaceutical products. The study was carried out in three different phases. In Phase I, three indigenous strains of B. cereus MMIC 1, MMIC 2 and MMIC 3 were isolated and identified from the garden soil samples followed by their molecular characterization and phylogenetic analysis. Phase II included the production of lipopeptide biosurfactants from these native strains using different agro-industrial wastes as carbon source. All three strains showed significant biosurfactant production potential. The optimum conditions for biosurfactant production were 37°C, 7th day of incubation, 0.5 % NaCl and pH 6.5. The corn steep liquor was found to be the best carbon source for the maximum yield. These biosurfactants were classified as cationic lipopeptides. The lipopeptide biosurfactants were safe/noncytotoxic, thermally stable (50°C) and tolerated acidity (pH=2). In Phase III, evaluation of these biosurfactants exhibited significant antibacterial activity against S. aureus, E. coli, P. aeruginosa and K. pneumoniae. Moreover, they also possessed excellent antifungal activity against A. niger and C. albicans and antiviral activity against NDV. The biosurfactants had the ability to emulsify olive oil, mustard oil and canola oil. They also showed very good antioxidant activity. Besides, they served as an excellent adjuvant in inactivated AIV-H9N2 vaccine and boosted humoral immune response of broiler and layer birds comparable to oil adjuvanted vaccine. Consequently, the study offers an insight for the exploration of new bioactive molecules from the soil. It was concluded that lipopeptide biosurfactants produced from three native strains of B. cereus may be recommended as safe bio-products in food and pharmaceutical industry over commercially available synthetic surfactants in future.
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ڈاکٹر شہزاد احمد بطور محقق و نقاد

ڈاکٹرشہزاد احمد بطور محقق و نقاد
(الف)اُردو نعت پاکستان میں: ایک جائزہ
شعبۂ نعت کا ہر گوشہ رسول کریم صلی اللہ علیہ و آلہٖ وسلم سے محبت اور عقیدت کے اظہار کا ذریعہ ہے۔ ڈاکٹر شہزاد احمد نے نبی کریم صلی اللہ علیہ و آلہٖ وسلم سے محبت کے اظہار کے لیے اپنے تحقیقی مقالے (نعتیہ شاعری کا تحقیقی جائزہ پاکستان کے خصوصی حوالے سے )یعنی ’’اُردو نعت پاکستان میں‘‘ کو وسیلہ بنایا ہے۔ یہ موصوف کا پی ایچ ڈی کا مقالہ ہے جس پر جامعہ کراچی نے ڈاکٹر یٹ کی سند سے نوازا ہے۔ یہ مقالہ موضوعات کی اہمیت اور معلومات کے استناد کی بدولت اُردو نعت شناسی کا ایک اہم سنگ میل ہے۔ کوئی محقق اس مقالے سے صرف نظر کرکے نعت کی حالیہ روایت سے کماحقہ آشنا نہیں ہو سکتا۔ اس مقالے کی تکمیل ۲۰۱۲ء میں ہوئی۔ حافظ عبدالغفار حافظ نے اس کی جو تاریخ نکالی ہے، وہ ملاحظہ ہو:
کیجیے حافظ رقم تاریخ اجرائے سند
روشنی طبع ’’اُردو نعت پاکستان میں‘‘
۲۰۱۲ء
-iنعت گوئی کا تحقیقی و تجزیاتی مطالعہ
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النزعة الصوفية في شعر خوشحال خان

This article focuses on the various aspects of Khushal's mystical poetry. Khushal was well-read and had a lot of scholarly exposure. He also spent time in the company of great scholars. All these factors contributed to his mystical poetry. I. According to him the servant of God knows himself. In other words those people know themselves who know Allah. Ii This world becomes a mirage for those who believes in Allah and the world seems a useless place to them. Iii. There is a universe in the heart of a “darwesh”. Iv. There are two types of mind; one is worldly and the other is spiritual. They both consider each other as the same. V. True love does not depend on wisdom but it depends on the passion of the individuals. In short, Khushal khan khattak has expressed great mystical thoughts in his poetry for the benefits of all and sundry, and invites them to think about their near future and see what is happening around them and what will be the answer of that questions which would be asked on the day of resurrection.

Constructivist Approach to Development of Criteria for Selection of Contents for Teaching English in Secondary School Class Ix-X

The study was conducted to investigate the opinion of the parents (Class IX & X students), English teachers, students of secondary classes and experts in curriculum field about the purpose of studying English and selection of contents accordingly. In addition the experts in curriculum field were also consulted on the procedures and processes used at present for selection of content. The focus was on four major areas i.e. social forces in society /community values and purposes, knowledge and its treatment, the nature of learning and the learner and human growth and development that are fundamental to any curriculum. Content and construct reliability and validity of the questionnaires were ensured through pre-testing as well as by obtaining experts opinion. For data collection a sample of 1000 English teachers, 2000 students, 2000 parents and 50 curriculum experts from all the four provinces and the federal capital was selected by adopting multi-stage random sampling procedure. It was ensured that the sample represents the population from rural and urban areas, Urdu and English mediums and public and private schools for both male and female in the right proportion. The key conclusions based on the descriptive and statistical evidences indicated that the people of Pakistan attach great importance to values and purposes related to religious teachings and want to promote these through schooling. It also revealed that the people are very keen to learn English as it is the language of knowledge and higher learning in all fields. The findings of the study indicated that in the present textbooks of English speaking is least emphasized as compared to other skills. The findings of the study also gave a fairly good idea of themes and sub-themes that the majority of population wants to include as content of English for classes IX–X. Based on statistical analysis of the data, criteria for selection of contents for teaching English in Secondary Schools has been developed and weightage suggested for each which have been validated by the Textbook Boards and Curriculum Bureaus in the country.