MicroRNAs (miRNAs) are short, endogenous and non-protein coding RNAs that are 18-26 nucleotides (nt) in length. The main function of the miRNAs is to negatively control the protein coding sequences at post-transcriptional level. By nature majority of the miRNAs are evolutionarily conserved. This conserved nature provides the logic to predict the new miRNAs orthologus in a number of plant species. In plants, miRNAs are involved in almost all biological processes from growth and development to biotic and abiotic stresses with metabolism, apoptosis and cell signaling. In the current dissertation, various computational tools were used for the profiling and characterization of new conserved miRNAs and their targets in four important vegetables of Solanaceae viz: tomato (Solanum lycopersicum L.), potato (Solanum tuberosum L.), eggplant (Solanum melongena L.) and chilli (Capsicum annuum L.). Consequently, a total of 393 miRNAs (belonging to 364 miRNA families) were discovered from the mining of 7,63,564 express sequence tags (ESTs). Of which, 120 miRNAs (110 miRNA families) were from potato, 109 miRNAs (106 miRNA families) from tomato, 88 miRNAs (81 miRNA families) from chilli and 76 miRNAs (67 miRNA families) were from eggplant. In this study, a total of three miRNA genes were found as pre-miRNA clusters, where, two belong to chilli (can-mir-8019 and can-mir-8036) and one is from potato (stu-mir-6164). One potato miRNA family stu-mir-8123 was also found both as an antisense (mir8123a) and sense (mir8123b). A total of thirty randomly selected miRNAs were subjected to reverse transcription-PCR (RT-PCR) validation studies. Furthermore, a total of 1592 protein targets were also predicted for these newly profiled miRNAs. These predicted protein targets were classified as: transcription factor, stress related, disease related, growth and development, hypothetical protein, transporters, signaling pathways, metabolism and structural protein. All of these newly identified miRNAs were reported in tomato, potato, eggplant and chilli for the first time. These results will serve as reference data to improve the regulation, management, and modification of these economically important crops at the molecular level. This will also help us to improve solanum plants for production and biotic and abiotic stress tolerance in the near future.
ایمان کا ایک بنیادی جز اس بات کا متقاضی ہے کہ نہ صرف اس بات پر یقین کامل ہونا چاہیے کہ حضور اکرمﷺبحیثیت خاتم النبیین اللہ تعالیٰ کے آخری نبی ہیں بلکہ اس کے ساتھ ساتھ آپﷺ کی ذات مبارکہ سے انتہا درجے کا عشق ہونا بھی ضروری ہے اور آپ کی تعلیمات پر عمل پیرا رہنا ہر صاحب ایمان مرد عورت پر فرض ہے۔ بحیثیت مسلمان ہمیں حضور پاک ﷺکی سیرت مبارکہ کے مختلف گوشوں کا مطالعہ کرنے کی ضرورت ہے تا کہ اپنی زندگی میں ان اصولوں کی پیروی کی جائے جو ہمارے لئے اسوہ حسنہ ہیں۔ لین دین کے معاملات سے لے کر حقوق العباد تک، عبادات سے لے کر ایمانیات و روحانیات تک الغرض شعبہ زندگی و عبادات سے متعلق ہر ممکنہ پہلوؤں تک ہماری یہی کوشش رہتی ہے کہ ہم سنت رسولﷺ کی پیروی کریں اور رسول اکرمﷺ کی تمام سنتوں کی مکمل طور پر پیروی کی جائے جو بدعات سے پاک ہوں۔ قرآن کے مطلوب و مقصود کے عین مطابق ہوں اور حضور اکرمﷺ کی اصل تعلیمات و تشریحات پر ہی مبنی ہو۔دین کے ایک طالب علم ہونے کی وجہ سے چونکہ ہم سیرت النبیﷺ کے مطالعہ کی اہمیت سے انکار نہیں کر سکتے۔ چنانچہ ہماری کوشش رہتی ہے کہ ہماری علمی تراث میں سیرت نگاری پر جتنا لیٹریچر مرتب کیا جا چکا ہے اس کا ایک تاریخی جائزہ لیا جائے۔ مصادر سیرت کے ہر ہر جز کی بنیاد کے فہم کو سمجھا جائے اور ایک عجمی ہونے کے ناطے ہمیں جہاں عربی مصادر سیرت نگاری کا مطالعہ کرنا درکار ہو گا وہیں اردو زبان میں شائع شدہ سیرت نگاری کا ایک تفصیلی و تحقیقی (بشمول...
The very existence of theology was a prelude to the competition of philosophy and the support of religion. Other religions, for the sake of legitimacy of their religion, have been raising objections to Islam in all respects. The mixture of nations, non-Arab influences, the negligent rhetoric of scholars and shaykhs, and the erroneous beliefs and practices of the people were tarnishing the clean face of Islam. In the time of Ibn Taymiyyah, there was an emphasis on theology. Theologians specialize in their knowledge and art of rationalism and logic. Their method consisted of reasoning and inference, rational and logical proofs. In contrast to them, the Hanbalis were holding the line of reasoning from the apparent meaning of verses and hadiths and texts. In the case of debates and debates, the weight of the theologians would be heavy and the opponent would be considered ignorant. Ibn Taymiyyah paid homage to the authenticity and supremacy of the Qur'an and Sunnah in such a way that he made critical commentary and analysis on philosophy and Greek logic and reasoned weaknesses and errors. One-half or two-thirds of his total writing services on the subject of speech and logic. This shows that Ibn Taymiyyah was feeling the need of defending Islam in this regard because in his time these sciences and arts had become very popular and common practice. The far-reaching effects of his thoughts and opinions in this regard have been felt in every age. The issues in which he has differed are the result of his research, jurisprudence and ijtihad, and constant reflection. The basis and foundation of which is the Quran and Sunnah, the interaction of companions and speculation. In these Ijtihad issues of hiss, there is a collection of arguments and proofs related to the Quran and Sunnah. In this article, an introductory and analytical study of Ibn Taymiyyah's fundamental differences will be presented.
The Bathymetric study of the Tiyon formation, which conformably lies in between Laki and Kirthar Formation around Laki Range is carried out to construct the paleoenvironment interpretation, worldwide correlation and assigned the Standard Chronostratigraphic age. The bathymetric study of the Tiyon formation indicates three zones with their characteristic particular species are: - Ø Brackish water zone (shelf neritic) Elphidium, Nonion Ø Shallow water zone (outer neritic) Quinqueloculina, Robulus Ø Deep water zone (inner neritic) Gyroidina, Rosalina Faunal assemblage and planktonic / benthic ratio suggests that the deposition of the Tiyon formation have took place under marine condition, indicating a near-shore deposition under a very shallow to bathyl zone with clear warm water condition in tropical region. The abundant presence of planktonic foraminifera in the lower part of the Tiyon formation indicates the connection of depositional basin with the open sea, while this condition does not exist in the upper part of the formation. The Tiyon formation contains a variety of stratigraphically important benthic and planktonic species which includes 140 species, 35 planktonic and 120 benthonic forams, belonging to 31 genera & 15 families. The recognized planktonic zonation includes Globigerinatheka semiinvolta Zone, Truncorotaloides rohri Zone, Orbulinoid beckmanni Zone, Globigerina frontosa Zone, Hantkenina aragonensis Zone and Acarinina pentacamerata Zone. These zones verify the Late Ypresian to Early Bartonian age to the Tiyon formation rather than Late Ypresian to Lutetian. The benthic assemblage shows distinct affinities with the Lutetian fauna of Sor Range, (Baluchistan), Raki Nala and Nammal Gorge (Salt Range), Hampshire Basin (England), Paris Basin (France), Gulf Coastal Region (USA), Belgium and Kutch (India). The Tiyon formation is correlated with Pir Koh Limestone (Lower part of Drazinda Shale), Basal Shale unit of Kirthar Formation (Baluchistan), upper part of the Ghazij Shale, Chorgali, Sakesar, Kuldana and basal part of the Kohat Formation. Total number of species and specimen have been plotted graphically to indicate the dominancy of different faunal zone over on another. Temperature is also a determining factor in the study of bathymetric study. Some characteristic species which are exclusively tropical and live in warm temperature also have been discussed in this thesis.