Search or add a thesis

Advanced Search (Beta)
Home > Purification and Characterization of Proteins Important for Homologous Recombination in Eukaryotes

Purification and Characterization of Proteins Important for Homologous Recombination in Eukaryotes

Thesis Info

Access Option

External Link

Author

Rana Salman Anjum

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2013

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/498

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727048403

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


Successful repair of DNA double strand breaks is crucial; failure of which can lead to tumourigenesis including breast/ovarian cancer. BRCA2 (breast cancer susceptibility protein type 2) and RAD51 from different specie interact in a process called homologous recombination (HR) during the repair of such kind of insults to DNA. BRCA2 being a large protein molecule has different sites of interaction for other proteins like DMC1 (disruption of meiotic control 1). In the present study, we have purified a distinct region in human BRCA2 close to DMC1 binding site which has been shown to undergo CDK (cyclin dependent kinase) based phosphorylation in our kinase assays. Characterization involving dynamic light scattering, limited proteolysis and circular dichroism spectroscopy techniques revealed this region to be mostly disordered. We have also attempted the successful purification of DMC1 (different from the previous method of its purification) employing C-terminus of DMC1 as binding partner for increased solubility. Further studies included the characterization of Trypanosoma brucei BRC repeats (TbBRC) and Rad51 interaction. We observed no interaction of Trypanosoma brucei Rad51 (TbRad51) with its BRC repeats upto 7 BRC repeats based on our in vitro pull down assays. The comparison of human RAD51 and TbRad51 structures highlighted critical differences of residues at hydrophobic cavities for the binding of phenylalanine from BRC repeat. We predicted that TbBRC repeats, collectively, might have WD-40 β-propeller structure. The purification of Trypanosoma brucei 7 BRC repeats rendered this protein highly soluble and stable based on circular dichroism spectroscopy which also revealed the protein having poly-proline structure, a characteristic of unordered proteins. Moreover, we have predicted multiple phosphorylation sites in TbRad51 and compared them with other eukaryotic Rad51 orthologues to get insights into a possible regulatory mechanism for Rad51.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

سید صباح الدین عبدالرحمن

آہ! سید صباح الدین عبدالرحمن!!
مسند شبلی و سلیمان اجڑ گئی
جناب سید صباح الدین عبدالرحمن ناظم دارالمصنفین و اڈیٹر معارف ہم سب کو چھوڑ کر اب خدائے رحمان کے آغوش رحمت میں چلے گئے، گذشتہ ماہ ۱۸؍ نومبر کو ٹیلیفون اور ریڈیو سے دن میں دو ڈھائی بجے کے درمیان ان کی المناک شہادت کی خبر دارالمصنفین میں بجلی بن کر گری اور ان کے متعلقین و وابستگان دارالمصنفین کی امیدوں اور آرزوؤں کے خرمن کو خاک و سیاہ کرگئی، مولانا سید سلیمان ندویؒ، مولانا عبدالسلام ندویؒ اور مولانا شاہ معین الدین احمد ندویؒ کے بعد دارالمصنفین کی بزم علم و ادب اسی چراغ سے روشن تھی لیکن افسوس کہ باد حوادث کے جھونکوں نے اسے بھی گل کردیا:
صبح تک تو نے نہ چھوڑی وہ بھی اے باد صبا
یادگار گل شمع تھی کل تک جو پروانے کی خاک
وہ بالکل ٹھیک تھے، ۱۰؍ نومبر کو لکھنؤ روانہ ہونے سے پہلے فجر بعد ہی مجھے نومبر کے معارف کے شذرات دیے اور ۹ بجے دن میں انھیں کاتب کے حوالے کرنے کی تاکید کی اور عین روانگی کے وقت فرمایا کہ ۱۹؍ نومبر کے بعد واپس ہوں گا، میں ان کی مشایعت کے لیے ان کے پیچھے ہوگیا تو مڑکر میری جانب دیکھا اور فرمایا کہ بیٹھیے! میں نے مصافحہ کے لیے ہاتھ بڑھایا تو انھوں نے مصافحہ کرتے ہوئے خدا حافظ کہا اور آگے بڑھ گئے، میری طبیعت نہ مانی اور میں ان کو رخصت کرنے کے لیے پھاٹک تک گیا، وہاں دوسرے رفقاء اور وابستگان دارالمصنفین بھی ان کو رخصت کرنے کے لیے پہلے سے موجود تھے، سب سے رخصت ہوکر وہ رکشہ پر بیٹھ کر روانہ ہوگئے، ۲۰؍ نومبر کو جمعہ تھا، جمعہ چھوٹ جانے کے اندیشہ سے وہ اس دن سفر نہیں کرتے، اس لیے خیال تھا کہ ۲۱؍ نومبر...

Why Do We Believe in God? An Analysis of the Motives of the Believing Behaviour in Human Beings

This article explores the motives of the human believing behaviour. The author postulates that to believe in God is natural and not to believe is a deviation from the true and pure human nature. This fact has, also, been admitted by many philosophers, psychologists and geneticists. A brief debate with reference to philosophy, anthropology, psychology and genetics has been presented to have a review the opinions of some eminent philosophers, psychologists and anthropologists about the believing behavior of the human nature. The traces of the religiosity of the primitive tribes without exception are a further evidence for the said fact. Some evidences have been presented from history and also from the examples of some living primitive tribes of Australia and Africa to accentuate the stance that to believe in God is a natural, innate, instinctual motive in the human nature. Author also quotes certain verses from the Qur’an to confirm the conformity of the historical, philosophical, psychological and genetical facts and findings with the Qur’anic stance about the believing behaviour of the human nature. The motives behind human behaviour in believing God are counted by the author as: rationality, anxiousness for God and the Life hereafter, Love of God, Affiliation with the native culture, Influence and Inspiration, Religion: A Remedy or Solution and Preaching in Terms of addresses.

Dynamics of Exchange Rate and Stock Prices: A Study on Emerging Asian Economies

The Purpose of this study is to explore the behavior of exchange rates in five Asian economies; namely Pakistan, India, Indonesia, Korea and Sri Lanka. The causality between capital and currency markets has been investigated in the first section of study. In second section, the link between exchange rate and economic variables has been scrutinized, while in the third section, forecasting performance of economic models has been compared with that of random walk and autoregressive integrated moving average model. Using Granger Causality test and Johansen Cointegration, the causality between stock and currency markets has been explored. Link between macro economic fundamentals and exchange rates has been investigated using ordinary least square method and Johansen’s cointegration, while forecasting performance of economic models has been compared with that of random walk and autoregressive integrated moving average model using one graphical and four statistical measures. These measures are Perfect Forecast Line (PFL), Root Mean Square Error (RMSE), Mean Absolute Error (MAE), Median of Absolute Deviation (MAD) and Success Ratio (SR). Nature of short run causality between stock and currency markets has been found different in different countries. In Pakistan and Sri Lanka, causality runs from stock market to currency market while feed back relationship has been found in case of Indonesia and Korea. In India, causality running from exchange rate to stock market has been found significant. However, no long run causality between stock and currency markets has been found in sample economies. Thus these two financial markets support asset market theory in the long run. However, regression analysis proves that economic variables are not senseless, whereas Johansen cointegration technique affirm the existence of long run relationship between exchange rate and macro economic variables. Johansen’s cointegration reports three cointegrating equations in Pakistan, India, Korea and Sri Lanka while two cointegration equations in case of Indonesia. Vector Error Correction Mechanism has been applied to gauge the speed of adjustment in relationship between exchange rate and macroeconomic fundamentals. Lastly predictive capacity of economic fundamentals based models namely Purchasing Power Parity, Interest Rate Parity and Adhoc model has been compared to that of Random Walk and Autoregressive Integrated Moving Average Model. In the sample forecasting has been used for comparison. Predictive capacity has been investigated using one graphical method called Perfect Forecast Line and four statistical methods. Statistical xiimethods include Root Mean Square Error, Mean Absolute Error, Median of Absolute Deviation and Success Ratio. All the four measures support fundamentals based approaches in all the sample economies except Indonesia where Random Walk Model has the power to beat fundamentals’ based approaches on the basis of all the four measures of statistical evaluation.