A set of 16 sugarcane genotypes comprising two check cultivars (CP-77/400 and Mardan-93) were assessed for repeatability, genetic gain and path coefficient analysis during 2012-14 and 2013-15 at Sugar Crops Research Institute (SCRI) Mardan, Khyber Pakhtunkhwa, Pakistan. The data were recorded on growth, cane, quality and yield traits for three crop seasons. Analysis of variance showed significant differences among genotypes, crops and genotypes x crops interaction. Repeatability (h2 broad sense) under plant crop, for different characters showed varying levels and it was moderate forinternode length (43%), cane yield (41%), number of nodes (39%), cane length (39%), millablecane (35%) and 2nd plant height (30%). Low repeatability was noted for 2nd tillering (12%) and 1st tillering (10%) under plant crop. Under ratoon crop, moderate repeatability was noted for 2nd tillering (47%), 1st tillering (39%) and internodes length (34%). Low repeatability was noted for brix (28%), cane yield (25%), cane diameter (23%), 1st plant height (19%), millablecane (17%), number of node (16%), recovery (16%) and cane length (15%) under ratoon crop. Across crops low repeatability was noted for internode length (26%), number of nodes (23%), 2nd tillering (14%) and 1st tillering (10%). Genetic gain under plant crop was higher for cane length (36.53 cm), 2nd plant height (31.84 cm) and 2nd tillering (12.98 tillers per 9 m2).Under ratoon crop, the genetic gain was higher for 2nd tillering (54.86 tillers per 9 m2), 1st tillering (40.88 tillers per 9 m2) and 1st plant height (15.63 cm). Genetic gain across crops was higher for 2nd tillering (15.52 tillers per 9 m2), cane length (9.55 cm) and 1st tillering (9.24 tillers per 9 m2). Under plant crop, highly significant and positive correlation of 1st tillering (rg = 1.00 , rp = 0.85), 2nd tillering (rg =0.96, rp =0.83), 1st plant height (rg =0.89, rp =0.77), 2nd plant height (rg =0.95, rp = 0.81), cane length (rg =0.90, rp = 0.76), number of nodes (rg =0.79 , rp = 0.67), internode length (rg =0.80, rp =0.74) and millablecane (rg =0.96, rp = 0.87) was noted with cane yield at genotypic and phenotypic levels. Similarly brix showed positive and highly significant phenotypic correlation with POL (rp =0.84) and recovery (rp = 0.71). Under ratoon crop, highly significant and positive correlation of 1sttillering (rg = 0.89 , rp = 0.81), 2nd tillering (rg = 0.92 , rp = 0.84), 1st plant height (rg = 0.86 , rp = 0.75),2nd plant height (rg = 0.96 , rp = .78), cane length (rg = 0.97 , rp = 0.69), internode length (rg = 0.77 , rp = 0.71), recovery (rg = 0.83 , rp = .64) and millablecane (rg = 0.85 , rp = 0.67) was noted with cane yield at genotypic and phenotypic levels. Brix showed positive and highly significant phenotypic and genotypic correlation with POL (rg = 0.99, rp = 0.98) and recovery (rg = 0.68, rp = 0.65). POL also has highly significant and positive correlation with recovery (rg = 0.72, rp = 0.70) at both the levels. Across crops, highly significant and positive correlation of 1st tillering (rg = 0.78 , rp = 0.70), 2nd tillering (rg = 0.86 , rp = 0.76), 1st plant height (rg = 0.95 , rp = 0.73), 2nd plant height (rg = 1.00 , rp = 0.77), cane length (rg = 0.77, rp = 0.63), internode length(rg = 0.85 , rp = 0.77) and cane diameter (rg = 1.00 , rp = 0.72) was observed with cane yield at phenotypic and genotypic levels. Millablecane showed highly significant and positive correlation at genotypic level while significant at phenotypic level (rg = 0.64, rp = 0.57) with cane yield. Brix showed highly significant and positive correlation with POL (rg = 1.00, rp = 0.95) and recovery (rg = 0.66, rp = 0.67) at genotypic and phenotypic levels. POL also has highly significant and positive correlation with recovery (rg = 0.74, rp = 0.79) at both the levels. Path analysis showed direct positive phenotypic effect on cane yield by 2nd tillering (P1,10 = 0.12), 2nd plant height (P2,10= 0.13), number of nodes (P3,10= 0.14), internode length (P4,10=0.32), brix (P5,10= 0.39), purity (P7,10=0.36) and millablecane (P9,10=0.39)under plant crop. However at genotypic level direct positive effect on cane yield was showed by 2nd tillering (P1,10=0.21), 2nd plant height (P2,10=0.42), number of nodes (P3,10=0.03) and millablecane (P9,10=0.63. Under ratoon crop, path analysis showed direct positive phenotypic effect on cane yield by 2nd tillering (P1,10=0.28), 2nd plant height (P2,10=0.04), cane length (P3,10=0.33), internode length (P5,10=0.32), cane diameter (P6,10=0.08), recovery (P8,10=0.06) and millablecane (P9,10=0.37). The direct positive genotypic effect on cane yield was exhibited by 2nd tillering (P1, 10= 0.16), 2nd plant height (P2, 10=0.40), cane length (P3,10=0.07), internode length (P5,10=0.24) and recovery (P8,10=0.73). Across crops, direct positive phenotypic effects on cane yield was showed by 2nd tillering (P1,10=0.20), 2nd plant height (P2,10=0.27), cane length (P3,10=0.19), internode length (P5,10= 0.28), recovery (P8,10=0.42) and millablecane (P9,10=0.05), however cane length (P3,10=2.36) and recovery (P8,10=1.94) had direct positive genotypic effect on cane yield. GenotypeMS-91-CP-523 had the highest path index values of 240.39 and 439.69 and performed better than rest of the genotypes under plant and across crops, respectively. Under ratoon crop genotype MS-2000-Ho-360 had the highest path index value of 141 and performed better than rest of the genotypes. Results further suggested that path analysis technique combined with development of path index could be successful in selection of sugarcane genotypes for improving overall selection approaches. The parameters with more broad sense heritability and genetic gain can be exploited in sugarcane breeding programs. The parameters having direct effect on cane yield must be given more importance in the breeding and selection strategies. Research should be focused on the selection of genotypes which has good performance both under plant and ratoon crops conditions. The genotypes with good performance may be tested further.
ایمان لانے کے بعد انسان پر سب سے پہلے عبادت کا ادا کرنا لازم ہے ہر مذہب میں عبادت کا ایک خاص طریقہ ہوتا ہے جو مخصوص طریقے کے ساتھ ادائیگی کا حکم دیا جاتا ہے اسی طرح اسلام میں بھی نماز، روزہ، حج اور زكوة عبادات کی مختلف طرق ہیں اصل عبادت کی غایت یہ ہے کہ معبود صرف اللہ تبارک وتعالیٰ ہی کو ماننا ، صرف اسی کی عبادت کرنا ہر چیز میں اسی سے مدد طلب کرنا اسی کو حاجت روا اور مشکل کشا سمجھنا اسی کو مالک، خالق اور رب تسلیم کرنا اسی سے التجاء کرنا، ہر چیز کے لئے اسی کو پکارنا اور یہ یقین رکھنا کہ اللہ کے سوا کسی کے دائرہ اختیار میں کوئی چیز نہیں ہے اگر وہ نفع پہنچانا چاہے تو اسے کوئی روکنے والا نہیں ہے اور اگر ضرر پہنچائے تو اس کو کوئی ہٹانے والا نہیں ہے ہر طرح کی عبادت مثلاً قیام، رکوع، سجدہ صرف اسی کے لئے خاص ہے اور کسی اور کے سامنے جھکنا جائز نہیں۔
انسانوں سے اللہ تعالیٰ نے انکی تخلیق سے پہلے ایک وعدہ لیا تھا جس کا ذکر قرآن مجید میں یوں مذکور ہے:
The downloading of the legal rulings on the facts and their transfer from abstraction to application needs a deep philosophical look from Mujtahid and Mufti. Among the things that must be taken into account consider individual differences between taxpayers because these differences may require a change in the Shari'a ruling by the change of the person who owns the incident according to its characteristics and conditions, from the clues that it embraces and requires this change. This indicates that individual differences between taxpayers should be taken into account during tinnitus litigation which is seen as a pilgrimage to the origins of the Sharia and its general rules and its effects were manifested and manifested in the methods and methods of fundamentalists and jurists.
A study, presented in the dissertation, described clinical and genetic characterization of nineteen consanguineous families (A-S) featuring various forms of inherited skin disorders. Fifteen of these families (A-I, K-P) showed various forms of isolated skin anomalies, while the remaining four families (J, Q-S) exhibited distinct forms of syndromic conditions. Isolated X-linked ichthyosis (XLI) was identified in four families (A-D). Initial marker analysis revealed two distinct interstitial deletions at chromosome Xp22.3. SNP array fine mapped the underlying deletions to ~ 1.67 Mb (family A, B, C) and ~ 1.62 Mb (family D). Different forms of isolated scaling skin phenotype, with autosomal recessive inheritance, was identified in five consanguineous families (E-I). Genotyping using microsatellite markers and haplotype analysis established linkage in the family E, segregating ichthyosis vulgaris, to a previously known gene FLG at chromosome 1q21.3. Subsequently, Sanger sequencing identified a novel homozygous nonsense variant (c.10459A>T; p.Arg3487*) in the third exon of the FLG gene in affected individuals. In family F, with ichthyosiform erythroderma, genetic delineation by exome sequencing revealed a previously reported nonsense variant (c.1630C>T; p.Gln544*) in the ALOXE3 gene. In the third family, segregating scaling phenotype, SNP genotyping and exome sequencing identified a novel gene CLUH carrying a homozygous missense variant (c.2852G>A; p.Arg951His) in affected members. Two other families (H and I), segregating autosomal recessive form of ichthyosis, failed to show linkage to the known genes. Abstract Clinical and Molecular Characterization of Human Hereditary Skin Disorders in Consanguineous Families XXVIII Pure hair and nail ectodermal dysplasia, with autosomal recessive transmission, was observed in an inbred family J. Genotyping established linkage in the family at chromosome 12p11.1-q21.1. Sanger sequencing identified a novel homozygous nonsense variant (c.404C>A; p.Ser135*) in the HOXC13 gene. Clinically various forms of isolated hypotrichosis was observed in six consanguineous families (K-P). Sequencing of a panel of genes failed to reveal potential pathogenic variants in two families (K, L), segregating autosomal dominant form of hair loss disorders. Direct sequencing of the gene LPAR6 in the family M identified a previously defined missense variant (c.562A>T; P.Ile188Phe) causing hypotrichosis with wooly hair. The in-silico studies of mutated LPAR6 protein verified aberrant receptor activity and downstream phospholipid signaling resulting in hair disorder, with curly phenotype. The conventional homozygosity mapping using microsatellites failed to identify linkage to known genes/loci in two other families (N, O). Exome sequencing in the family P wasn’t successful in identifying a homozygous pathogenic sequence variant causing hair loss. The study, described in the dissertation, elaborated genetic characterization of three consanguineous families segregating syndromic forms of hair loss disorders. In the family Q, with hypotrichosis and Juvenile Macular Dystrophy, haplotype analysis established linkage to gene CDH3 on chromosome 16q. Sequence analysis identified a novel homozygous in-frame deletion variant (c.764_766delACT; p.255delTyr) in the CDH3 gene. In family R and S clinical investigation found the condition Woodhouse Sakati syndrome (WSS) and Nonphotosensitive trichothiodystrophy (TTDN), respectively. Exome sequencing identified a novel truncating homozygous variant Abstract Clinical and Molecular Characterization of Human Hereditary Skin Disorders in Consanguineous Families XXIX (c.270delA; p.Lys90Asnfs8*) in the gene DCAF17 and splice site variant c.339+1G>A in the gene MPLKIP in the family S. Structural investigation of mutated CDH3 p.255delTyr and DCAF17 p.Lys90Asnfs8* predicted atypical interactions with associated proteins. cDNA analysis of mutated MPLKIP c.339+1G>A verified unusual splicing event resulting in intron retention and setting up syndromic attributes in the family S.