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Home > Role of Aquatic Plants and Indigenous Microbes in Improving Phytoremediation Efficiency of Industrial Sludge

Role of Aquatic Plants and Indigenous Microbes in Improving Phytoremediation Efficiency of Industrial Sludge

Thesis Info

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Author

Khilji, Sheza Ayaz

Program

PhD

Institute

University of the Punjab

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2014

Thesis Completion Status

Completed

Subject

Plant Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/2629/1/3102S.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727109928

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The aim of the present research was to assess the metal phytoextraction potential of some selected plant species (Hemarthria compressa L., Lemna minor L. and Typha angustifolia L.) growing on industrial sludge along with resistant microbial strains (Aspergillus terreus, Aspergillus niger, Bacillus sp. and Acinetobacter sp.) either alone or in combinations. Based upon the result of preliminary experiments, the sludge concentrations selected for the actual greenhouse and field experiments were 30 and 60% along with control (0%). Keeping in view the metal resistance efficiency of microbes, the experiment comprised of seven treatments i.e., C (control without microbes), F1 (Aspergillus terreus), F2 (A. niger), F1+F2 (A. terreus + A. niger), B1 (Bacillus sp.), B2 (Acinetobacter sp.), B1+B2 (Bacillus sp. + Acinetobacter sp.), respectively. Six independent experiments (4 green-house and 2 field) were launched simultaneously. Physicochemical analysis of initial sludge samples revealed that tannery and paper sludge had a high pollution load due to higher values of pH, conductivity, total dissolved solids (TDS), sodium chloride (NaCl), biochemical oxygen demand (BOD), chemical oxygen demand (COD) and metals like Cr, Cd, Zn, Cu and Pb. After 90 days of plant growth in sludge, the growth was significantly decreased with increase in the concentration of sludge. On the other hand, in sludge concentrations along with microbial inoculum, plants showed the maximum shoot length, roots, leaves and fresh/dry weight in F1+F2 and F+B treatment as compared to all other tested treatments. Physicochemical parameters also showed a substantial decrease except TDS that showed an increasing trend. Minimum values of all were observed in combined microbial treatment as compared to control and other treatments. The chlorophyll content of leaves in all selected plants decreased with increasing the concentration of industrial sludge. Plants growing in different concentration of sludge, supplemented with combined fungal treatments in pot experiments and F+B treatments in field experiments, showed higher chlorophyll contents than the plants growing in other treatments. Results revealed that all the tested plants species showed a higher accumulation of essential (Ca, K, Na and iii Mg) and heavy (Cd Cr, Cd, Zn, Cu and Pb) metals at 60% concentration of sludge as compared 0 and 30% after 90 days of plant growth. The metal uptake was higher in their shoots as compared to roots in case of all the plant species, in both paper and tannery sludge. Further, the uptake of metals was significantly higher in F1+F2, B1+B2 and F+B treatment as compared to rest of the treatments i.e. F1, F2, B1, B2, F, B alone and control treatment. The metal extraction efficiency of the experimental plants was observed to be in the order of T. angustifolia > H. compressa > L. minor. The translocation factor and enrichment coefficient indicated that a higher amount of heavy metals was absorbed by the plants than was present in the sludge. The least bioaccumulation was observed for Na in all the cases. Typha angustifolia proved to be a better metal accumulator than all other hydrophytes. Biochemical analysis of T. angustifolia and H. compressa showed that in 60% TS with combine fungal and bacterial inoculum, superoxide dismutase (SOD) and catalase (CAT) activity was high i.e. in the order of 60% TS> 30% TS> 0% TS. The results from this work in the light of contemporary literature indicates that a probable genetic modification at cellular level resulted in an acquisition of metal tolerance that was also evident in enhanced biochemical activity of antioxidant enzymes, uptake of high metal contents and ultimately better plant growth in all the treatments as compared to control. The results of these experiments emphasize that efficient pollution hyperaccumulators can be used for commercial and large-scale cleaning and bioremediation of tannery sludge. The plants can then be harvested easily and incinerated. Constructed wetlands offer the treatment benefits of natural wetlands in a more controlled environment, if developed along the industrial units. The treatment processes within such a system will be self- sustainable, requiring little input of energy, chemicals and operator maintenance
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روگ ہجر دا دل نوں لا سٹیا

روگ ہجر دا دل نوں لا سٹیا
سانوں عشق نے مار مکا سٹیا
نہ خواباں تک اِنہاں سفنیاں نیں
یوسف وچ کھوہ دے پا سٹیا
ہوئے عشق دے وچ فقیر راجے
جامہ حلیہ سب بدلا سٹیا
سائنس نہیں کوئی عشق دا حل کر دی
سائنس داناں مغز کھپا سٹیا
جدوں عشق زلیخا دے کھچ کیتی
یوسف مصر دے وچ لیا سٹیا
مہینوال تجارتاں چھڈ بیٹھے
پاسہ عشق نے سب پرتا سٹیا

COMPARISON OF PATELLAR MOBILIZATION AND TAPING IN PATIENT WITH PATELLOFEMORAL PAIN SYNDROME

Background of the Study: To compare patellar taping and mobilization plus conventional therapy for reducing knee pain in patients with patellofemoral pain syndrome (PFPS). Methodology: Controlled trial with 50 participants divided into two groups who received different treatments for 6 weeks: Group A had patellar taping and iliotibial band stretching, while Group B had patellar mobilization and quadriceps strengthening. Participants received three treatment sessions weekly for six weeks. The VAS was used to conduct pre and post-test pain evaluations for groups A and B. Results: Knee pain decreased in PFPS patients receiving patellar taping (Group A) or mobilization (Group B) using VAS (p<0.05). All treatments are effective for PFPS. The VAS scores after 6 weeks of post A and post B treatments assessed. After 6 weeks of taping, mean = 0.76±0.83. After 6 weeks, mean and SD = 1.20±1.12 from patellar mobilization. No significant difference between means (p = 0.12, α ≤ 0.05). Insignificance. Conclusion: The result of the study indicated that after 6 weeks of treatment for both patellar taping and patellar mobilization were effective in decreasing pain in PFPS.

Study of Mathematical Models for Blood Flow Through Arterial Stenosis

Study of Mathematical Models for Blood Flow Through Arterial Stenosis In this dissertation investigations are carried out for steady two-dimensional motion of an incompressible Newtonian and second grade fluid through the channel and tube. The hemodynamic behavior of blood flow is analyzed in the presence of an arterial stenosis. In the present study Cartesian and cylindrical coordinates are considered respectively for the flow in the channel and in an axisymmetric tube with heat transfer having stenosis of cosine shape. Length of stenosis is assumed to be large as compared to the semi width of the channel or the radius of the tube. The basic governing equations are the Navier-Stokes equations which are reduced to stream function formulation by using transformation. In the last two chapters blood is assumed to behave as an isothermal Oldroyd-B fluid through the channel and tube. By eliminating pressure gradient term form momentum equations, compatibility equation is obtained. The resulting compatibility and energy equations are highly nonlinear and solved analytically with the help of two methods namely the regular perturbation method (RPM) and the Adomian decomposition method (ADM). In case of regular perturbation method δ, which is the ratio of semi width of the channel or radius of the tube to the length of the channel or tube, is considered as a small parameter. The solutions thus obtained from the present analysis are represented in terms of streamlines, wall shear stress, zero wall shear stress, pressure gradient and temperature distribution on blood flow through stenosed channel and tube. Its worthy to mention that results presented in this dissertation are compared with already published results which ensures good agreement. It is found that wall shear stress, pressure gradient and temperature increases with the development of stenosis and causing separation and reattachment points in the region. It is found that even at low xiiivelocity separation is observed, if the thickness of the stenosis is increased. Detailed discussion and graphical representations are also provided.