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Role of Endophytic Bacteria Containing Carbonic Anhydrase in Improving the Photosynthesis and Plant Biomass of Cereals at Different Moisture Regimes

Thesis Info

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Author

Ana Aslam

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Agricultural Technology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/11611/1/Ana%20Aslam_Soil%20Sci_2019_UAF_PRR.docx

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727112185

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Climate change is one of the serious threats to food security throughout the world especially in arid and semi-arid regions, affecting agricultural productivity. Rapid changes in global climate such as alteration in rain fall pattern and increase in temperature lead to severe drought stress that limits the crop production by reducing photosynthetic rate and suppressing plant growth. Endophytic bacteria containing carbonic anhydrase may improve plant growth and elicit tolerance under water deficit stress by enhancing the photosynthesis in plants. Carbonic anhydrase (CA) catalyzes the reversible conversion of atmospheric carbon dioxide into bicarbonate, first substrate of photosynthesis in C3 and C4 plants. Therefore, present study was conducted to assess the potential of drought tolerant CA containing endophytic bacteria for improving photosynthesis and plant biomass of cereals under different moisture regimes. One hundred and fifty bacterial isolates were isolated form two cereals (wheat and maize) and tested for their ability to tolerate PEG-induced water deficit conditions. Fifty isolates exhibiting higher drought tolerance from each crop were further analyzed for CA activity. Ten drought tolerant isolates with higher CA activity were further assessed for growth promotion in wheat (C3) and maize (C4) plants. Isolates WR2, WS11 and WL19 showed higher photosynthetic rate and plant growth in both wheat cultivars; however, increase was more for Uqab-2000 than Fsd-2008 under PEG- induced water deficit conditions. Moreover, isolates MR17, MS1 and MG9 gave significant increase in photosynthesis and plant growth in both maize hybrids, especially for H2 hybrid under PEG-mediated water stress. Selected isolates from both crops were labeled with Gus and tested for plant growth promotion as well as colonization efficiency in wheat and maize under water deficit stress. Inoculation of selected isolates showed significant results for photosynthesis, growth and colonization efficiency of wheat and maize under well watered (100% FC) and stressed (70 and 40%) conditions especially for Uqab-2000 and H2. In the same ways, isolates WR2, WS11 and WL19 gave significant results for growth, physiology and yield of wheat under field condition where water deficit stress was induced by skipping irrigation at tillering, flowering and grain filling stage. On the other hand, inoculation with isolates MR17, MS1 and MG9 improved growth under normal and stressed conditions which were induced by withholding irrigation at vegetative and reproductive stage of maize. Selected isolates also proved to be efficient auxin producer and p-solubilizer under normal and stressed conditions. These isolates were identified as Bacillus sp. In separate study, it was observed that endophytic bacterial isolates carrying CA activity AR4 and AR14 (belonged to Microbacterium sp. and Psychrobacter sp., respectively) also stimulated the expression of various genes and transcription factors in Arabidopsis thaliana under normal as well as PEG-induced water deficit conditions. Therefore, it can be suggested that inoculation of endophytic bacterial isolates (WR2, WL19 for wheat and MR17, MG9 for maize) is good for enhancing photosynthesis and plant biomass under water deficit conditions. Moreover, multi-site field experiments for these isolates are suggested for evaluating the successful performance in field. However, molecular studies are required to confirm role of bacterially synthesized CA in photosynthesis.
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وادی سینا

وادیِ سینا

جس وادی مقدس طویٰ کا ذکر قرآن میں ملتا ہے اس کی وسعت قلبی واضح نظر آ رہی تھی ۔ گرمی سے نڈھال پیاسی وادی نے اپنی چونچ بحیرہ احمر میں ڈال رکھی تھی ،بحر ہند کا پانی جزیرہ نما عرب کے یمنی ساحلی علاقے راس منہلی اور براعظم افریقہ کے راس سیاں کے درمیا ن کوئی بیس میل کی ایک تنگ گھاٹی سے گزر کر افریقہ اور ایشیا کے درمیان حدِ فاضل کھینچتا ہے جو وادیٔ سینا پہنچ کر دوحصو ںمیںمنقسم ہو جا تا ہے مشرقی حصے کو خلیج عقبہ جبکہ مغربی حصے کو خلیج سویز کہا جا تا ہے ۔قاہرہ سے وادیٔ سینا جاتے ہوئے خلیج سویز کو عبور کرنا پڑتا ہے ۔اس حصے میں موسیٰ  ؑنے اپنی قوم کو فرعونی مظالم سے نجات کے لیے پار کرایا تھا اور اسی بحر میں فرعون منفتاح غرق ہوا تھا۔

مصر ،سعودی عرب،اسرائیل اور اردن کی سرحدیں خلیج عقبہ میں ملتی ہیں ۔سعودی عرب اسی ساحل ’پر نیوم‘ نامی بستی بسا رہا ہے ۔اس جدید بستی کے بارے میں یہ بات کہی جا رہی کہ یہاں عیش و عشرت کے وہ سارے ذرائع موجودد ہوں گے جن کے لیے مغرب و مشرق کے عیاش طبقات دنیا کے کسی بھی کونے میں جانے سے انکار نہیں کرتے ۔

جہاز نے سمت بدلنے کے لیے جب داہنا پنکھ نیچے کیا تو کھڑکی سے خلیج سویز ایک نیلے ربن کی طرح نظر آ رہی تھی ۔محمد علی نے اشارہ کیا کہ وہ پانی خلیج سویز ہے ۔انہوں نے نہر سویز کی لمبائی چوڑائی اور اس پر برطانوی ،امریکی اور فرانسیسی قبضے کی تاریخ بھی بیان کی او ر بڑے فخر سے جمال عبدالناصر کی بہادری اور اس نہر کے قومیانے کے عمل کو سراہا ۔ انہوںنے کہا کہ ۱۸۶۹ء...

اسلام اورہندومت میں احترام انسانیت ایک تقابلی جائزہ Respect for Humanity in Islam and Hinduism A Comparative Review

Every person in the world is respectable because he is a human being. All religions of the world have taught respect for humanity. It will not be wrong if we say that respect is the common heritage of humanity living in the world. Mutual respect is the secret and distinction of the development of nations. How many nations and cultures became enviable simply because they respected each other? In the present era, the dignity of humanity is being trampled everywhere. The murder market is hot everywhere. The standards of human respect are changing, sincerity, truth, human equality, and values ​​are being replaced by deceit, greed, envy, and selfishness. And it is done according to the resources. Man is the best of creation and legitimate respect and honor is his basic right. Therefore, no one can see his self-respect being undermined. That's why it has become very important to bring up topics like respect for humanity and explain the position and status of human beings in the light of religious books so that the respect and dignity of human beings are being trampled underfoot today, people should be made aware of the true status and status of human beings should be informed so that the importance of human blood can be estimated. An attempt will be made in this paper to present a comparative review regarding the respect and reverence that human has for Islam and Hinduism. Keywords: Respect for Humanity, Superior to all creatures, Religion, Islam, Hinduism, Respect, Self-respect

Exploration of Flora of District Bannu, Khyber Pakhtunkhwa, Pakistan

The research work aim to explore the flora of Bannu and get information and report the ethnobotanical knowledge of prevalent medicinal flora of the districtBannu and the adjoining area of FR-Bannu area in 2013-15. As a whole about 400 plant species belonging to 271 genera 90 families were collected, and conserved in Herbarium of Botany Department U.S.T, Bannu.The plants were collected from different villages, and organized alphabetically after identication, along with their botanical, English, local and family names, explanations, part used, flowering period, chemicals constituents, methods of applications of their parts and medicinal importance. Most of the plants reported here were wild some were cultivated. While other were wild as well as cultivated plants withe medicinal values. Similarly large number of plants were used asfodder, fuel, wood for furniture and also as making of ropes. There were found plants with edible fruits e.g. Mangifera indica, Pyrus malus etc. Several plants were classified as ornamental flora while some were of use as fencing and hedging.Out of400 collected flora 60 plants were observed as vegetables i.e. Lycopersicon esculentum, Allium cepa, Allium sativa, Brassica, Chenopodium, Solanum, Cucurbita,Capsicum, Mentha, Cucumis and Amaranthus whilst some plants are classified as cereal crops i.e. wheat, maize, oat and rice. In this way whole of the flora are classified as in various groups. Wild species (73), 4.0% Cultivated plants (45), 5.38% Ornamental plants (9),Indicator species (47) from 36 families (4.10%), Food values species (50) from 22 families(4.02%),. 48 Economically important flora (5.2%),Weed flora 186 plant species (45%), Poisonous plants (87) 54 genera(4.73%), Vegetables (60) plants species from 47 genera belonging to 26 families (13.58%),laticiferous plants 10 from 04 families( 02%), herbs (75%), shrubs (15%) and tree (10%),Aquatic plants (13%). fodder and forage species (14.75%), Fuel (7.3%), Fences (7.02%), Veterinary medicines (5.3%), Roof making (2.13%), furniture making (4.82%), Honeybees species (5.11%), while(1.77%) species considered to be sacred by locals, toothbrushing species (1.77%), basket making species (1.18%), sweeper making species (1.18%), carpet making species (1.18%), condiments and spices (2.25%), cricket bat making species (1.18%), (1.18%) species used after mehindi, soil binder species (3.10%), green tea species (0.59%), dye making species (0.59%) and (0.59%) chewing gum species. Most of the plants belong to the following families Poaceae (45.349%) having 27 genera (10.588%) and 39 species (10.076%), followed by Asteraceae (33.721%) with 22 genera (8.627%) and 29 species (7.494%),Papilionaceae (33.721%) 15 genera (5.882%) 29 species (7.494%), Solanaceae (26.744%) 11 genera (4.314%) 23 species (5.943%) Brassicaceae (22.093%) 11 genera (4.314%) 19 species (4.910%), Euphorbiaceae (17.442%) 6 genera (2.353%) 15 species (3.876%), Polygonaceae (16.279%) 5 genera (1.961%) 14 species (3.618%),Amaranthaceae (1516.1%) 7 genera (2.745%) 13 (3.359%), Apiacea (13.953%) 12 genera (4.706%) 12 species (3.101%), Rosaceae (11.628%) 5 genera (1.961%) 10 species (2.584%), Boraginaceae (10.465%) 6 genera (2.353%) 9 species (2.326%), Cucurbitacae (10.465%) 6 genera (2.353%) 9 species (2.326%) , Cyperaceae (9.302%) 5 genera (1.961%) 8 species (2.067%), Malvaceae (9.302%) 6 genera (2.353%) 8 species (2.067%), Lamiaceae (8.140%) 6 genera (2.353%) 7 species (1.809%), Moraceae (8.140%) 3 genera (1.176%) 7 species (1.809%), Myrtaceae (6.977%) 6 genera (2.353%) 6 species (1.550%),Apocynaceae and Liliaceae (5.814%) 4 genera (1.569%) 5 species (1.292%), and Caryophyllaceae (5.814%) with 3 genera (1.176%) 5 species (1.292%). Other important families were Ranunculaceae, Chenopodiaceae, and Plantaginaceae (5.814%) having 2 genera (0.784%) and 5 species (1.292%) each, Potamogetonaceae (5.814%) with 1 genus (0.392%) and 5 species (1.292%), Convolvulaceae (4.651%) 2 genera (0.784) 4 species (1.034%), Juncaceae (4.651%) 1 genus (0.392%) 4 species (1.034%), Verbenaceae (4.651%) 3 (1.176%) 4 species (1.034%), Nyctaginaceae and Gentianaceae (3.488%)with 3 genera (1.176%)and 3 species (0.775%) each, Mimosaceae and Zygophyllaceae (3.488%) Both having 2 genera (0.784%) 3 species (0.775%), Rhamnaceae (3.488%) and Rutaceae (3.488%) each one have 1 genus (0.392%) and 3 species (0.775%). Similarly Alismataceae,Caesalpiniaceae,Cupressaceae,Hydrocharitaceae,Papaveraceae and Scrophulariaceae (2.326%) contributed 2 Genera (0.784%) and2 species (0.517%) each, while Cactaceae,Equisetaceae,Fumariaceae, Salicaceae, Taxaceae and Typhaceae (2.326%) have 1 genus (0.392%) and 2 species (0.517%) each. The remaining families, Agavaceae, Aizoaceae, Amaryllidaceae Anacardiaceae, Araceae, Aristolochiaceae, Asclepiadaceae, Asphodelaceae, Aspidiaceae, Begoniaceae, Bignoniaceae, Cannabinaceae, Capparidaceae, Combretaceae, Crassulaceae, Cuscutaceae, Cycadaceae, Dennstaedtiaceae, Elaeagnaceae, Fagaceae, Iridaceae, Linaceae, Magnoliaceae, Meliaceae, Oleaceae, Orchidaceae, Orobanchaceae, Oxalidaceae, Piperaceae, Portulacaceae, Primulaceae, Punicaceae, Resedaceae, Rubiaceae,Salvadoraceae, Sapindaceae, Sterculiaceae, Tamaricaceae, Tiliaceae, Urticaceae, Vitaceaehaving family percentage (1.163%) with 1 genus(0.392%) and 1 species(0.258%) each.So for as the important genera contributing more species are concerned it was reported that 73 familieshave more than 2 genera with total contribution of201 species, for instance Euphorbiaceaeand Polygonaceaewere at the top withgenera likeEuphorbia and Polygonum respectively with 9 species (3.529%) having a percentage of (12.329%) in top 73 genera of district Bannu. Other important genera were Brassica with6 (8.219%)species (2.353),Lathyrus, Potamogeton, Solanum with 5 (6.850) having a percentage of 1.961 Amaranthus, Chenopodium, Jancus, Ranunculus, Prunus, Datura, having 4 (5.480) having a percentage of 1.569 Achyranthes, Carthamus, Heliotropium, Cyperus, Hibiscus, Ficcus, Morus, Astragalus, Melilotus, Medicago, Plantago,Lolium, Ziziphus, Rosa, Citrus andPhysalis with 3 (4.110) species each having a percentage of 1.176. while Alternanthera, Nerium, Calendula, Eclipta, Launaea, Sonchus, Taraxacum, Nonea, Neslia, Raphanus, Silene, Spergula, Opuntia, Ipomoea, Convolvulus, Citrullus, Cucurbita, Cucumis, Fimbristylis, Equisetum, Jatropha, Fumaria,Mentha, Ocimum, Allium, Acacia, Trifolium,Veronica, Alopecurus,Aristida,Avena,Echinochloa, Hordeum,Phragmites, Poa,Sorghum, Rumex, Salix, Cestrum,Nicotiana, Withania, Taxus, Typha, Vitex and Fagonia having 2species (2.740%) having a percentage of 0.784. While the remaining 13 famlies have one genus with single species each. The present study also deals with poisonous plants of the area, in which 87 poisonous spp of plants belonging to 54 genera were reported. Important genera reported were Brassica 6 species (11.11%), Lathyrus 5 spp(9.26%),Euphorbia and Astragalus were with 4 spp with 7.40%. Ranunculus, Jatropha, Solanum, Sorghum and Datura were with 3 spp (5.56%) while Taxus, Melilotus, Chenopodium, Amaranthus and Allium have 2 spp (3.70%) each. These 15 genera contribute 48 species (55.17 %) while the 39 genera has single species each and contribute 44.83% to the total poisonous flora of the research area. Other plants were Cannabis sativa, Datura stramonium L., D. metel L., Jatropha curcas, Taraxacum officinale, Taxus baccata L., and Xanthium strumarium. In the present study the genetic diversity of the species in relation to their stem, leaves, root, flower, inflorescence, calyx. Chaorlla, androcium, gynoecium, fruit and seed of the local flora of Bannu and FR Banuu were also studied, it was found that the most common color are grey, brown and milky. The researcher has also noticed great diversity in vegetative and reproductive characteristics of the local flora.