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Home > Role of Plant Growth Promoting Rhizobacteria and Chemical Fertilizers on Plant Growth, Oil Content and Quality of Safflower and Canola and Their Potential for Biodiesel Production

Role of Plant Growth Promoting Rhizobacteria and Chemical Fertilizers on Plant Growth, Oil Content and Quality of Safflower and Canola and Their Potential for Biodiesel Production

Thesis Info

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Author

Nosheen, Asia

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2014

Thesis Completion Status

Completed

Subject

Botany

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/2419/1/3026S.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727122729

Similar


Experiments were conducted to evaluate the role of plant growth promoting rhizobacteria (PGPR) alone and supplemented with chemical fertilizers to improve quantity and quality of safflower (Carthamus tinctorius L.) and canola (Brassica napus L.) with perspective to biodiesel production. First experiment of the series was conducted to evaluate the effect of PGPR viz. Azospirillum brasilense and Azotobacter vinelandii and chemical fertilizers (Urea and DAP) alone and in combination (under axenic conditions) on enzymes activities of rhizospheric soil in addition to general impact on growth of safflower cvv. Thori and Saif-32. The PGPR were applied as seed inoculation at the rate of 10 6 cells/mL prior to sowing. Chemical fertilizers were applied at full (Urea 60 Kg ha -1 and Diammonium phosphate (DAP) 30 Kg ha -1 ), half (Urea 30 Kg ha -1 and DAP 15 Kg ha -1 ) and quarter doses (Urea 15 Kg ha -1 and DAP 7.5 Kg ha -1 ) during sowing. The colony forming units (cfu) of Azospirillum and Azotobacter were higher in the presence of quarter dose of chemical fertilizers. Activities of soil enzymes viz. urease and phosphatase were enhanced by Azotobacter in combination with quarter doses of chemical fertilizers and Azospirillum in combination with half dose of chemical fertilizers respectively whereas; root proliferation was enhanced by Azotobacter and Azospirillum supplemented with half and quarter doses of chemical fertilizers. The 2 nd experiment was focused on the effect of PGPR and chemical fertilizers under field conditions on plant growth, seed yield, oil contents and quality of safflower with perspective to biodiesel production. The indole acetic acid, gibberellic acid, oil contents, oil/protein ratio and seed phenolics were improved by Azospirillum with half dose of chemical fertilizers. Azospirillum in combination with quarter dose of chemical fertilizers improved the fatty acid profile, oil quality and amino acids contents with parallel increase in biodiesel yield whereas; Azotobacter in combination with quarter dose of chemical fertilizers improved seed crude protein and induced protein of 130 KDa and 100 KDa. Seed nutrients viz. Ca +2 , K + were increased by Azotobacter and Azospirillum supplemented with half dose of chemical fertilizers. In the 3 rd experiment effects of chemical fertilizers, Azospirillum and Azotobacter were studied on plant growth, seed yield and oil quality of canola (Brassica napus L.) var. Pakola pertaining to biodiesel production. Significant improvements in seed yield ixand seed size were recorded in chemical fertilizer treatment while oleic acid (C18:1) was improved by Azospirillum treatment with parallel decrease in erucic acid contents. Azotobacter showed maximum increase in seed oil content with concomitant decrease in seed glucosinolate and moisture content. Chemical fertilizers and Azospirillum decreased the oil acid value and free fatty acid (%FFAs) contents with concomitant increase in seed protein and biodiesel yield (93 % and 92% respectively). Protocol for the production of safflower biodiesel was optimized using 1 H NMR, FT- IR, GC-MS and refractometer techniques. The biodiesel samples prepared by base catalyzed transesterification reaction showed maximum yield (97.84%) at 0.5% catalyst concentration with 6:1 methanol/oil ratio at 65°C as quantified by 1 H NMR technique with minimum (1.41) refractive index and improved physico-chemical properties. The GC/MS analysis showed the presence of five major faty acid methyl esters. Improvement in separation and quantification of fatty acid methyl esters in safflower oil was achieved using comprehensive two-dimensional GC (GC×GC). The GC×GC separation accomplished by the combination of SLB-IL111 with IL59 column phases provided excellent separation of FAME standard mixture and also safflower FAMEs were well separated and quantified in a short run of 16 min. It is inferred that PGPR can supplement the chemical fertilizers upto 50%–75% in order to obtain optimum growth and yield of safflower and use of advanced technologies improved biodiesel and fatty acid analyses being more rapid, precise and cost effective.
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صرع کے دوروں کا الزام

صرع کے دوروں کا الزام
مستشرقین اپنے مذموم عزائم کو برواکار لاتے ہوئے آپ ﷺ کو مصروع ثابت کرنے کے لیے چند واقعات کا سہارا لیتے ہیں جو ان کی ذہنی اختراع اور باطل تاویلات کا نتیجہ ہیں جبکہ ایسے واقعات دیگر مقدس ہستیوں کو بھی پیش آئے لیکن مستشرقین و منکرین سب کو چھوڑ کر صرف حضور ﷺ کے سر الزام دھرتے ہیں اور دوسری برگزیدہ شخصیات کا نام تک نہیں لیتے کیونکہ ایسا کرنے سے ان کی اپنی محبوب ہستیاں بھی زد میں آتی ہیں۔ وہ واقعات جن سے یہ مستشرقین آپ کو مصروع ہونے کی ناکام کوشش کرتے ہیں درج ذیل ہیں ۔
۱۔اسپرنگر حضرت آمنہ کے فرشتوں کو دیکھنے کو مرگی سے تعبیر کیا اور یہی مرض حضور کو ورثہ میں ملا‘ کا الزام دھر دیا ۔ علامہ محمد احسان الحق سیلمانی لکھتے ہیں کہ حضرت آمنہ ؓ ، حضور ﷺ کی والدہ ماجدہ نے اپنے رویا میں فرشتوں کو دیکھا جو انہیں احمد ﷺ کی خوشخبری دینے اور آپ کا نام مبارک تجویز کرنے آئے تھے ۔‘‘ سپرنگر نے کہا کہ فرشتوں نے کیا بشارت دینی تھی ، حقیقت میں حضرت آمنہ کو ضعف دماغ اور صرع کی بیماری تھی ۔ حضرت آمنہ ؓ تو فرشتوں کو دیکھے اور یہی کہے کہ یہ فرشتے ہیں لیکن مستشرقین اسے صرع کہتے ہیں ، عجیب منطق ہے۔ جو ساتھی آپ کے ساتھ مدت العمر رہے انہوں نے کسی موقع پر بھی مصروع نہیں کہا ۔ یہ سب کچھ پیغمبر اسلام اور آپ کے گھرانہ کی شان گھٹاناچاہتے ہیں(۲) دوسرا واقعہ یہ ہے کہ آپ ﷺ اپنے بچپن میں اپنے رضاعی بہن بھائیوں کے ساتھ بکریاں چرانے گئے کہ آپ کا رضاعی بھائی اپنے والدین کے پاس دوڑتا ہوا آیا کہ دو سفید پوش مردوں نے میرے قریشی بھائی کا لٹا کر اس کا...

تعمیر شخصیت کے بنیادی اصول قرآن و سنت کی روشنی میں

Considering the context of this sermon one can see how Hazrat has adapted to the general tendencies of society. Where people are completely immersed in worldlinees. Today our situation is that every one is engaged in the pursuit of wealth and the worldliness has become the target of all. Hazrat Ali (RA) introduced the Quran as a guide in such a case. Calling Quran the book of life, he called it a solution of all the problems of the human society.

Impact of Integrated Application of Biochar and Nitrogen on Biochemical Composition of Maize and Wheat Crops

Application of biochar and nitrogen not only improves cereal crops productivity but also the nutritional quality of grains. Field trials on the impact of biochar and N amendments on the soil physicochemical properties, microbial activity and nutritional and antinutritional components of maize and subsequent wheat crop was conducted in district Buner, Khyber Pakhtunkhwa, Pakistan during 2014-15. Biochar was applied at the rate of 0, 5, 10, 15 and 20 ton/ha along with N at the dose rate of 0, 100, 150 and 200 kg/h for maize and 0, 80, 120 and 200 kg/ha for wheat crop. The immediate effect of biochar and N amendments was assessed on the chemical composition of maize (cv. Azam) grains whereas the residual impact of biochar coupled with fresh application of N was determined on subsequent wheat (cv. Sahar) crop. The soil physicochemical properties and microbial activities were evaluated in the beginning, reproductive stage and at maturity of both crops. The crops were harvested at full maturity and the grains were analyzed for proximate composition, starch content, total protein and fractions, minerals, fatty acids, phytic acid and tannins contents. The average values of soil pH, electrical conductivity (EC), organic matter (OM), N, P, K and microbial biomass-carbon (MB-C), microbial biomass-nitrogen (MB-N) and C/N ratio of the composite soil before sowing of maize crop were found to be 8.2, 0.37dS/m, 0.95%, 1.74 mg/kg, 3.45mg/kg, 61.5mg/kg, 190 μg/g, 19 μg/g and 10.12 μg/g, respectively. The biochar and N levels significantly (p≤0.05) increased soil pH whereas soil OM was substantially enhanced only with biochar at the reproductive stage of maize. At maturity stage of maize crop, MB-C and MB-N were significantly affected by biochar and N treatments whereas soil pH, OM and EC were not significantly (p≥0.05) affected. Residual biochar significantly (p≤0.05) increased soil pH, OM, MB-C, MB-N and C/N at reproductive stage of wheat, while the effect of N levels was significant (p≤0.05) only for soil MB-C, MB-N and C/N ratio. At maturity stage of wheat crop soil OM, MB-C and C/N ratio was significantly (p≤0.05) increased with biochar residues and N applications, whereas soil pH and MB-N was only influenced by residual biochar. The proximate composition of maize grain was significantly (p≤0.05) affected by both biochar and N levels. Treatment combination of 20 t/ha biochar and 150 kg/ha N significantly (p≤0.05) increased the protein content of maize grains. Fat contents was higher with 20 t/ha biochar with no N and crude fiber content with no biochar and 200 kg/ha N application. Nitrogen free extract (NFE) was significantly (p≤0.05) increased with 15 t/ha biochar with no N application. Starch content of maize was highest with 15 t/ha biochar and 100 kg/ha N. Maximum amylose content (26.33%) was recorded in maize grains at treatment combination of 20 t/ha biochar with no N whereas amylopectin content was highest (77.02%) at 100 kg/ha N with no biochar. Maize grain albumin and globulin contents were highest at 20 t/ha biochar and 200 kg/ha N whereas glutelin and prolamine were highest with the application of 20 t/ha biochar and 120 xvi kg/ha N. Stearic acid, myristoleic, palmitoleic and linolenic acids contents were significantly (p≤0.05) higher at 20 t/ha biochar and 200 kg/ha N. Myristic acid content was highest (0.22%) with no biochar and 200 kg/ha N. Similarly, palmitic acid was maximum with the application of 10 t/ha biochar and 100 kg/ha N. The highest oleic acid content (35.15%) was found in maize grains at 10 t/ha biochar and 200 kg/ha N. Maize grain linoleic acid content was the highest (50.06%) with 15 t/ha biochar and 200 kg/ha N. Regarding minerals, maize grain K, P, Ca, Mg and Mn content were maximum at 10 t/ha biochar and 200 kg N/ha whereas the Cr and Ni contents were significantly (p≤0.05) higher at no biochar and 200 kg/ha N application. An increasing trend of phytic acid and tannins content of maize grains were observed with biochar and a reversal of the influence was noted with N application. Residual levels of biochar coupled with fresh application of N substantially influenced the chemical composition of wheat crop. The average wheat grain crude protein was highest with 20 t/ha biochar and 200 kg N/ha, while the average crude fat content was maximum at 20 t/ha biochar residues and no N. The highest average total starch content was recorded at 15 t/ha residual biochar and 120 kg N/ha, while amylose content was maximum at 20 t/ha biochar residues and no N. Average amylopectin content was highest at no residual biochar and 200 kg N/ha. Grain average albumin, globulin, glutelin and prolamine contents were highest at 20 t/ha biochar residues and 200 kg N/ha. Regarding fatty acid profile of wheat grains, the average values of myristic and stearic acids were highest at no residual biochar and at 200 kg N/ha. The average contents of palmitic acid, linoleic acid and linolenic acid were maximum at 15 t/ha biochar, while palmitoleic, oleic and cis- 11- eicosenoic acids were highest at 20 t/ha residual biochar. In response to N applications, the highest average values of palmitic acid, palmitoleic acid, cis- 11- eicosenoic acid and linoleic acid were obtained at 200 kg N/ha, whereas heptadecanoic acid and oleic acid were maximum at 120 kg N/ha. The grain average K, Ca, Mg and Mn contents were highest at 10 t/ha biochar residues and 200 kg N/ha. The highest average values of Fe, Cu, Cr and Ni were examined in wheat grains with the application of 200 kg N/ ha with no residual biochar. Grain P content was highest with 15 t/ha biochar residues and 200 kg N/ha. The wheat grain average phytic acid and tannins content were highest in the absence of N with 20 t/ha residual biochar. Overall, biochar application at the rate of 20 t/ha coupled with 200 Kg N/ha resulted in the highest concentration of majority of the nutrients in both maize and wheat grains. However, the concentration of antinutritional factors were lowest at 200 Kg N/ha in the absence of biochar. It was concluded that biochar amendments coupled with N application is an effective tool to enhance the nutritional quality of cereal grains.