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Home > Role of Zinc Ascorbic Acid and Cedrus Deodara Root Oil in Prevention Cyclophosphamide Induced Nephrotoxicity in Rat Model

Role of Zinc Ascorbic Acid and Cedrus Deodara Root Oil in Prevention Cyclophosphamide Induced Nephrotoxicity in Rat Model

Thesis Info

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Author

Kazi, Dr. Maria

Program

PhD

Institute

Isra University

City

Hyderabad

Province

Sindh

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Biochemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9916/1/Dr%20%20maria%20Kazi_Isra_2016.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727127153

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BACKGROUND: Nephrotoxicity is one of the adverse effects of cyclophosphamide which is a cytotoxic alkylating agent that may be nephrotoxic both in humans and animal models by generating reactive oxygen species. Therefore, purpose of this study was to investigate whether antioxidants like zinc and ascorbic acid as well as most prominent traditional-based plant material like Cedrus deodara root oil could decrease the intensity of toxicity caused by cyclophosphamide in albino rats. OBJECTIVES: · To evaluate the histomorphological changes in cyclophosphamide-induced kidney damage in albino rats. · To determine the role of zinc and ascorbic acid in cyclophosphamide-induced renal damage in rat model. · To assess the phytoprotective role of cedrus deodara root oil in cyclophosphamide-induced kidney damage. · To observe the renal function tests in the controls and experimental groups of animals. · To observe the changes in the antioxidative enzymes in the controls and experimental groups of animals. MATERIALS AND METHODS: The present study was conducted at Isra University, Hyderabad and Al-Tibri Medical College, Karachi. The animals used for this study were adult albino rats of (Wistar strain) 200-250 g of either gender and were procured from the animal house of Al-Tibri Medical College, Karachi. They were maintained on well balanced laboratory diet. The animals were kept in 12/12 hour light-dark cycle and water was provided freely x throughout the experimental period. The temperature where the animals were placed was maintained between 25 – 30˚C. total sixty (60) albino rats was taken in this experimental study. Animals were divided into six groups and each group comprised of ten (10) rats placed in separate cages. Control group A were given normal saline 1 ml/ day intraperitoneally for 21 days. Experimental group B received single dose of cyclophosphamide 1mg/100g intraperitoneally on first day of study. Experimental group C received 1mg/100g cyclophosphamide and 500 mg/100g ascorbic acid orally daily for 21 days. Experimental group D received cyclophosphamide 1mg/100g single dose on first day along with zinc-sulphate 0.1mg/100g daily for 21 days. Animals of experimental group E received cyclophosphamide 1mg/100g on first day intraperitoneally along with ascorbic acid 25 mg/100g, zinc sulphate 0.05 mg/100g and Cedrus deodara root oil 0.05 mg/100g intraperitoneally for 21 days. At the end of 21 day of treatment protocol, rats were anesthetized by ether inhalation and scarified for biochemical and histopathological examination. Blood sample 5.0 ml was taken through cardiac puncture and transferred into sterilized gel tubes for estimation of biochemical parameters. Kidneys were removed out after laparotomy and preserved at -80˚C for tissue slicing. Thick tissue sections 5μ were prepared by rotary microtome. The tissue sections were mounted on well cleaned and gelatinized slides and were stained with Hematoxylin and Eosin according to Bancroft and Stevens method for histopathological findings. The data obtained was then analyzed on SPSS version 21.0 (IBM, corporation, USA) P-value 0.05 was taken as significant. xi RESULTS: The results of present study showed that serum superoxide dismutase (SOD), glutathione peroxidase (GPX), reduced glutathione (GSSH), catalase, Malondialdehyde (MDA), creatinine, urea, uric acid (UA) and electrolytes like Na+, K+, Cl-, Ca++ and Mg++ were found statistically significant among the control and experimental groups of animals. Animals of group B when compared with control group A and other groups showed statistically significant difference of antioxidant enzymes (p<0.0001). experimental group F when treated with (cyclophosphamide, ascorbic acid and zinc sulphate) showed significant increase in antioxidant enzymes as well as significant improvement were seen in urea, creatinine and serum electrolytes (p=0.001). CONCLUSION: The present study revealed that the Cedrus deodara root oil exerted potent free radical scavenging property and showed potent antioxidant activity. Hence, it may be concluded that the mechanisms of nephroprotection by herbal agent Cedrus deodara root oil in cyclophosphamide treated animals could be due to its antioxidant and free radical scavenging activity.
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مولوی بشیر الدین

مولوی بشیرالدین
اسی مہینہ ہماری پرانی بزم کی ایک اہم اور قدیم یادگار مولوی بشیر الدین صاحب نے وفات پائی، وہ اپنے دور کی آخری نشانی تھے، ان کی ابتداء سرسید کی مخالفت سے ہوئی تھی، پھر ان کے حامی، مقلد اور ان کے مشن کے مبلغ بن گئے اور اپنی زندگی مسلمانوں کی تعلیمی خدمت کے لیے وقف کردی، اسلامیہ کالج اٹاوہ ان کا بڑا کارنامہ ہے۔
اخبار البشیر کے ذریعہ بھی انھوں نے بڑی خدمات انجام دیں ایک زمانہ میں وہ نہایت وقیع اخبار تھا اور اس کی آواز بڑی مؤثر تھی، تعلیم کے علاوہ بھی انھوں نے بہت سے تعمیری کام انجام دیئے، ان کی زندگی قومی کارکنوں کے لئے نمونہ تھی، وہ اگرچہ سرسید کی جماعت کے آدمی تھی، لیکن سیاسی خیالات میں آزاد اور قوم پرور تھے اور آخر تک اس مسلک پر قائم رہے۔ اب ایسے مخلص اور عملی انسان مشکل سے پیدا ہوں گے، ایک سو سال سے زیادہ کی عمر پائی، اﷲ تعالیٰ اس کہن سال خادم قوم کو اپنی رحمت و مغفرت سے سرفراز فرمائے۔ (شاہ معین الدین ندوی،جولائی ۱۹۵۶ء)

 

Authoritative Position of Investor and Scope of Liability in Modarabah Contract: Classical and Contemporary Jurists Perspective

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The Efficiency of Press Mud, a Sugar Mill By-Product, As an Alternative and Cheaper Source of Phosphorus Fertilizer in Semi-Intensive Fish Culture System

The commercial and conventional organic and inorganic fertilizers being used in Fish Culture System are usually very costly and sometimes their availability is also a big issue. Hence, there is a dire need of the time to search for alternate and cheaper sources to meet the need of the day and covering financial inflation, too. For this purpose, Sugar Press Mud (SPM), a by-product of Sugar Industry was taken into consideration to be a targeted organic Fertilizer / Manure in Semi-Intensive Fish Culture System taking Indian Major Carp, Labeo rohita (Rohu) as a selected fish. A series of experiments were conducted on SPM for its proper futuristic application to the fish ponds. These included its physico-chemical analysis; macro- and micro-nutrients profile along with heavy metal determination; temperature and pH influences on available phosphate releases from SPM and its further effects on fish survival and growth; determination of suitable application rates of SPM for Semi-intensive Fish Culture System and its comparison with other available, already in use manures like Cow dung (CD) and Poultry droppings (PD). The physico-chemical analysis of SPM samples including pH, total Alkalinity, total Hardness, Electric Conductivity and Total Dissolved Solids fulfilled the criteria for Aquaculture needs and were found in accordance with desirable ranges established by WHO, 1986; Boyd, 1990; FEPA, 1991; EPA USA, 1976 and UNECE, 1994. The multi-elemental analysis indicated that SPM contained luxuriously high amounts of macro-nutrients (N, P, K, Ca, Mg and S); moderate levels of micro-nutrients (B, Co, Cr, Cu, Fe, Mn, Mo, Na, Ni, Sr and Zn) availability along with the safer levels of toxic elements (Al, Ba, Cd, La, Li and Pb). The total and available released levels of phosphate from SPM samples at an application rate @ 8000 kg ha−1 were evaluated and found minimum at 0.01 mgL-1 to a maximum of 72.66 mgL-1 for total phosphate, respectively and from a minimum of 0.01 mgL-1 to a maximum of 4.13 mgL-1 for available phosphate, respectively. The effects of temperature and seasons on release of available phosphates trends from SPM as a Phosphate fertilizer @ 10,000 kg hac-1 at three different pH ranges i.e., 6.1-7.0, 7.1-8.0 and 8.1-9.0 in comparison with rock phosphate (RP) was evaluated. It was observed that the phosphate releases always increased periodically with high temperature in summer. However, amongst two fertilizers, press mud of sugar mills proved to be highly rich in the available phosphate-phosphorus and released it at a significantly and fairly higher rate as compared to the rock phosphate. It was further recorded that maximum release was observed in experimental aquaria fortified with press mud of sugar mills maintained at pH level 6.1-7.0. The most suitable pH ranges for efficient phosphate releases, physico-chemical suitability of water and for obtaining better survival and growth targets of Labeo rohita was also appraised which was found to be in the range 7.5-8.5. The best fertilization rates evaluation studies of SPM were undertaken which proved 8000 kg hac-1 at the first priority level followed by 10,000, 12,000 kg hac-1 while least with 6000 kg hac-1 with respect to P-PO4 release from SPM, physico-chemical suitability of water, survival and growth rates of Labeo rohita with a stocking density of 15000 Fish fry / hectare. The SPM compared in combination with Cow dung and Poultry droppings concluded that SPM (100%) was best followed by combination of SPM (67%) + CD (33%) and CD (67%) + SPM (33%) while the ranges observed in SPM (67%) + PD (33%) fertilization ratio were mostly unsuitable. The SPM feasibility in fish polyculture systems with a combination of Indian Major Carps and Chinese Carps revealed the best fish growths was shown by Hypophthalmichthys molitrix. The best survival rate was provided by Ctenopharyngodon idella followed by Labeo rohita, Catla catla, Cirrhinus mrigala and Hypophthalmichthys molitrix. On the basis of our research, Sugar Press Mud can be considered as a good optional organic futuristic Aquaculture fertilizer leading to more sustainable, economical and environment friendly systems due to its phosphates availability and nutrients recycling.