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Sageretia Thea Osbeck. Mediated Synthesis of Multifunctional Nanoparticles and Their Possible Applications

Thesis Info

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Author

Khalil, Ali Talha.

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Biotechnology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9408/1/Ali_Talha_Khalil_Biotechnology_2018_QAU_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727131472

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We successfully performed green process using the aqueous leaf extracts of medicinal plant Sageretia thea (Osbeck.) to produce multifunctional metal oxide nanoparticles. The biosynthesized Zinc oxide (ZnO), Iron oxide (Fe2O3), Nickel oxide (NiO), Cobalt oxide (Co3O4) and Lead oxide (PbO) nanoparticles were subjected to intensive physical characterization techniques like XRD, FTIR, Raman, EDS, SAED, HR-SEM and HR-TEM. Debye Scherer approximation was used to determine the size of the biogenic nanoparticles. Average sizes of the nanoparticles were calculated as 12.5 nm (ZnO), 29 nm (Fe2O3), 18 nm (NiO), 27 nm (PbO) and 20.03 nm (Co3O4). As synthesized nanoparticles were investigated for their possible biological applications. Antimicrobial, cytotoxic, enzyme inhibition, antioxidant and biocompatibility assays were performed. Antibacterial properties against 6 pathogenic bacterial strains using disc diffusion assay and their MIC’s were calculated. Significant antibacterial potential was revealed for ZnO nanoparticles. Furthermore, the effect of UV-illumination in the enhancement of antibacterial properties was studied. Agar tube dilution method for linear mycelial growth inhibition was used to determine the antifungal potential of biogenic metal oxide nanoparticles. Significant cytotoxicity was revealed against Artemia salina. Dose dependent cytotoxicity is reported for the biosynthesized nanoparticles against Leishamnia tropica (promastigotes and amastigotes) and HepG2 cell lines using MTT cytotoxic assay, while their biocompatibility was assessed against freshly isolated human macrophages and RBC’s. Median lethal concentration (IC50) values were calculated. Significant protein kinase inhibition is indicated by Fe2O3 nanoparticles while insignificant alpha amylase inhibition is reported for biosynthesized nanoparticles. Moderate DPPH radical scavenging activity while insignificant total antioxidant and total reducing potential was indicated. In addition, ZnO nanoparticles were deposited on the 3D porous substrate (nickel foam) to fabricate electrode material for supercapacitor applications. The fabricated NiF/ZnO electrode showed high specific capacitance of ~ 453 F g-1 at a relatively high current density of ~ 2 A g-1. The electrode also showed excellent cycling performance with 86% specific capacitance retention after 1000 cycles. Our results suggest that biosynthesized metal oxide nanoparticles can be used in diverse applications.
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ہم پر خدا کے لطف کی برسات ہو گئی


ہم پر خدا کے لطف کی برسات ہو گئی
رحمت نبیؐ کی شاملِ حالات ہو گئی

ہر حرفِ نعت مطلعِ دل پر طلوع ہوا
ہر صبحِ نو عطا مجھے اِک نعت ہو گئی

نعرہ لگایا ’’آمدِ حق‘‘ کا رسولؐ نے
باطل کو ہر جہان میں پھر مات ہو گئی

پیشِ نظر اگر نہ رہا اُسوۂ رسولؐ
نقدِ حیات نذرِ خرافات ہو گئی

عرفان! جس پہ مہرِ رسالت ہے ضوفگن
اُس کو خبر نہیں ہے کہ کب رات ہو گئی

اﻷداء اﻟﻤﺄﻣﻮر ﺑﻪ و ﺗﻄﺒﻴﻘﺎﺗﻪ اﻟﻔﻘﻬﻴﺔ

In the Islamic Law i. E. Quran and Sunnah the above mentioned topic has been discussed clearly and repeatedly to avoid any ambiguity in dealings in our daily life and agreements made between two parties or governments national or international levels. After intensive study I discussed and elaborated the said issue referring to the Qura’nic verses and sayings of the Prophet (SAW). The matter is of great importance; hence the Muslims Jurists have also given their valuable opinions in accordance with the Islamic Law which have been incorporated also to solve the issue. If we act upon these verdicts, we will be able to select the best among ourselves and form an ideal government and will discharge our duties honestly, and eventually our every act will show our responsibility to perform our duties and to give due share to the right person.

Prediction Based Greedy Routing Strategies for Wireless Networks of Moving Objects

Calculation and prediction of link duration between two nodes connected, as to create networks for communication system has always been a main area of high degree of importance. With the introduction of Ad Hoc networking concept along with its emerging subsets like MANETs, VANETs and FLYNETs. The issue regarding the calculation and prediction of the node''s link with reference to time is becoming more challenging because of the requirements of different strategies in different scenarios. As well as the changing topology and high speed of node had a great impact of link duration analysis. Geographic routing algorithms have the potency to work with a large network without maintaining the routing table of a node with complete route information, thus decreased the routing overhead. These routing strategies are incredibly suitable for the networks created by the VANETs with their high speeds and changing topology characterization. A number of researchers are involved with the development of routing strategies for Vehicular Ad Hoc Networks (VANETs), which can cope up with the uncertainties of the scenarios associated with the variable speed and high dynamics change of network topologies. With the advent of IPv6 and cheap availability of GPS, system''s geographic routing is becoming a solution for the problem associated with the possibility of the successful routing in VANETs. Disadvantage associated with the geographic routing is its reliability associated with the precise requirement of position information of the node. These networks are quite hard to handle in terms of the presence of such hindrances or may prove to be much more cost effective. Furthermore, time lagging factor’s presence in the routing table of the neighboring node because of the movement of the node position information is a reality which cannot be denied. Hence, the pattern of movement of a node is another issue which should be taken into account before the selection of routing nodes. Opportunely, according to our analysis vehicle movement is not complicated in highway scenarios. By using this, the significant observation, we had designed a movement pattern which can simulate the nodes moving in the highway scenario. Data set of a project group had been used. Project group had gathered data on a high way for bi directional traffic. Roads are divided into 1000 meter segments as to observe the movement pattern and then analysis had been performed. Our highway scenarios are able to capture different events and also connect the vehicles on the road from each other. We have suggested a fresh approach for geographic routing, which exploits the predicted locations of vehicles. We have proposed a stable link duration technique based on the prediction method by using the heading direction and present speed of a vehicle. This can effectively reduce the requested frequency of position update. Special tool in MATLAB has been designed for the testing different scenarios of VANETs by using different entity based mobility models.