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Home > Semen Characteristics, Preservation and its Use in Artificial Insemination of Red Jungle Fowl Gallus Gallus Murghi

Semen Characteristics, Preservation and its Use in Artificial Insemination of Red Jungle Fowl Gallus Gallus Murghi

Thesis Info

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External Link

Author

Allah Rakha, Bushra

Program

PhD

Institute

Pir Mehr Ali Shah Arid Agriculture University

City

Rawalpindi

Province

Punjab

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/8107/1/Bushra%20_Allah%20Rakha_HSR_2017_Reproductive_PhysiologyWM_PMAS_Rwp_22.10.2017.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727159101

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The Indian red jungle fowl (IRJF; Gallus gallus murghi) native to South-Asia is facing threats in its natural habitat and needs immediate conservation employing ex-situ and in-situ approaches. For ex-situ, in vitro conservation of IRJF, semen banking is one of the potential techniques that require an extender with appropriate cryoprotectants having adequate retrieval capacity for functional spermatozoa. Therefore, study was designed to evaluate semen production capacity (semen characteristics, timing, frequency of collection and seasonal changes), identification of efficient extender, cryo-damage estimation, permeable (glycerol, DMA, DMSO and DMF) for short and long term storage of semen and the use of non-permeable cryoprotectants (PVP and egg yolk) and fertility outcomes for ex situ in vitro conservation of IRJF germplasm. Semen was collected from eight male birds of IRJF (housed at main campus PMAS-AAUR) in a graduated plastic tube through abdominal massage and transferred to the laboratory for assessment of semen volume, initial motility and concentration. The ejaculates having at least 60% motility were processed for further experimentation. The qualifying ejaculates were studied for motility, volume, concentration, plasma membrane integrity, viability and acrosomal integrity of spermatozoa. Semen production in IRJF was quite low compared to domestic fowl but could be collected safely on daily basis preferentially in the evening. A number of diluents (Beltsville poultry, turkey, Lake, EK, Tselutin poultry and chicken semen extender) were tested against their efficacy to preserve IJRF spermatozoa in liquid (5ºC) and frozen state (LN2; -196ºC). The turkey semen extender was found superior compared to all xxvii 4 4 experimental extenders for storage in liquid and frozen state showing remarkably higher fertility rate. Cryopreservation with 11% glycerol caused maximum loss to motility (50%) followed by plasma membrane integrity (45%), viability (25%) and minimal to acrosomal integrity (20%). Glycerol optimization (11, 15 and 20%) study demonstrated the superiority (P < 0.05) of 20% glycerol in freezing for cryopreservability and fertility after artificial insemination. Various levels of DMA, DMSO, DMF and PVP (4, 6, 8, and 10%) were evaluated; 6% DMA, 8% DMSO, 8% DMF and 6% PVP maintained higher (P < 0.05) semen quality and fertility after artificial insemination compared to control treatment where spermatozoa were exposed to 20% glycerol. Among four levels of egg yolk (10, 15, 20 and 25%) evaluated; 15% egg yolk was superior (P < 0.05) for semen quality and fertility compared to the control. It is concluded that IJRF semen with maximum efficiency can be collected once in a day either in the morning or evening. The germplasm of IJRF can be conserved in liquid (for two days) and in frozen state (for indefinite period) using cryopreservation protocol based on DMA or DMF or DMSO or PVP and egg yolk can be used efficiently in artificial breeding program for conservation.
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محمد دین بھٹی

محمد دین بھٹی (۱۸۸۳۔۱۹۷۵ء) سیالکوٹ میں پیدا ہوئے۔ سکاچ مشن سکول سیالکوٹ اور سکاچ مشن کالج سیالکوٹ کے طالب علم اور علامہ اقبال کے ہم مکتب تھے۔ آپ مولوی میر حسن کے شاگردتھے۔ مولوی میر حسن اپنے گھر کا سودا سلف لانے کے لیے محمد دین بھٹی کو اپنے ساتھ بازار لے جایا کرتے تھے۔ منشی فاضل‘ مولوی عالم کے علاوہ ایم۔ اے انگریزی کرنے کے بعد سکاچ مشن سکول سیالکوٹ میں ۳۵۔۱۹۳۰ کے درمیان مدرس کے طور پر تقرری ہوئی۔ بعد میں مرے کالج سیالکوٹ میں عربی‘ فارسی اور اردو کے لیکچرار تعینات ہوئے۔ محمد دین بھٹی اقبال کے ہم عصر شاعر تھے۔ آپ کا شعری مجموعہ ’’ماء معین‘‘ شائع ہو چکا ہے۔ یہ مجموعہ کلام اقبال اور مولوی میر حسن کے نام سے معنون کیا گیا ہے۔(۲۰۷)راقم الحروف کی کوشش کے باوجود یہ مجموعہ کلام دریافت نہیں ہو سکا۔ نمونۂ کلام ملاحظہ ہو:

کوئی پوچھے کہ کیا ہوتی ہے تقدیر۔                                وہ تھی جو پہلے تھی تدبیر             

اگر تدبیر ہو قاصر بہ تکمیل                          تو تقدیر بھی ہوتی ہے تغیر             (۲۰۸)

۲۰۷۔ طلعت نثار خواجہ، ’’مرے کالج کے ادیب اساتذہ‘‘ ، مقالہ برائے ایم۔ اے اردو، لاہور ،پنجاب یونیورسٹی، ص:۳۷۰

۲۰۸۔ ایضاً ،ص: ۳۷۲

 

Muslim Religious Militancy: Causes and Cure

Islamic religious militancy is a matter of great concern for the Muslim and the non-Muslim world today. The analysis of the ideology of the militants reveals that they find the legitimacy of their military activities in the ideal of the establishment of an Islamic state to establish the universal rule of Islām, and in the specific interpretations of some Qur’ānic verses, Aḥādīth of the Prophet (r), and also from the establishment of the Islamic state in Madīnah by the Prophet (r), his the military expeditions and those of his companions against their opponents and from the treatment of our historians of the individual military campaigns against the Muslim regimes of their times. The Muslim militants also fight against their Muslim governments on the grounds that they are not the true Islamic governments. The militants do not bother to kill the common Muslim masses, who vote and support such rulers. They take it as collateral damage. The world naturally reacts to this cult, especially the west, being at the helm of the world politics. Not only do the West tries to crush the Islamic militants, across the world, but also, topple the Muslim democratic governments having any ideal of an Islamic Khilāfah. This frustrates the peaceful political activists and strengthens the military activists, further. To end this ongoing and mounting cult of religious militancy, it is necessary to review the specific and traditional interpretations of the academic sources of Islām: Qur’ān, Ḥadīth and Fiqh, regarding the legitimacy of militancy in Islām. Secondly, to remove their misconceptions, it is necessary to engage the militants in dialogue through a counter narrative, which the author tried to present here.

Growth Responses and Metal Accumulation Patterns in Fish Exposed to Chronic Metal Mixture Concentrations

Acute toxicity of 19 mixtures of iron, zinc, lead, nickel and manganese were determined in terms of 96-hr LC 50 and lethal concentrationsfor five fish species viz.Catla catla, Labeo rohita, Cirrhina mrigala,Ctenopharyngodon idella and Hypophthalmichthys molitrix under laboratory conditionsat constant pH (7), total hardness (200mg/L) and water temperature (30°C).The extent of metals bio- accumulation in fish body organs viz. gills, liver, kidney, fins, bones, muscle and skin at both 96-hr LC 50 and lethal concentrations were also determined. In the 2 nd phase, growth responses of five fish species were determined, separately, under chronic exposure of 19 mixtures of metals at sub-lethal concentrations (1/3 rd of LC 50 ) for 12 weeks. The bioaccumulation of metals in the fish body organs viz. gills, liver, kidney, fins, bones, muscle and skin were also determined before and after growth trails under the stress of 19 mixtures. All the five fish species showed significantly variable tolerance limits in terms of 96-hr LC 50 and lethal concentrations against 19 mixtures of five metals. Amongst 19 mixtures / treatments, the mixture of five metals (Fe+Zn+Pb+Ni+Mn) caused significantly higher toxicity to the fish, in terms of 96-hr LC 50 and lethal concentrations of 46.23±11.54 and 73.46±14.18mgL -1 , respectively. However, Pb+Mn and Zn+Pb mixtures were significantly least toxic to the fish with the mean LC 50 and lethal concentrations of 83.48±11.34 and 134.70±26.77mg L -1 , respectively. Regarding overall sensitivity of five fish species, Hypophthalmichthys molitrixwere significantly more sensitive to metals mixtures with a mean LC 50 value of 59.63±11.01mgL -1 while Labeo rohita showed significantly least sensitivity (81.73±12.73mgL -1 ).This significantly higher sensitivity of Hypophthalmichthys molitrix towards metal mixtures was followed by that of Cirrhina mrigala, Catla catla, Ctenopharyngodon idella and Labeo rohita. However, the difference between Catla catla and Cirrhina mrigala, for their tolerance limits (LC 50 ), were statistically non-significant. The overall sensitivity of all the five fish species, determined in terms of lethal concentrations, against various metals mixtures varied significantly. Regarding overall response of fish towards 19 mixtures, mean sensitivity of fish to the mixture of five metals (Fe+Zn+Pb+Ni+Mn) was significantly high (P<0.05), followed by that of four metalsmixtures (Fe+Zn+Pb+Mn) with statistically significant difference. Hypophthalmichthys molitrix were significantly more sensitive to metals mixture with themean lethal concentration of 97.85±16.20mgL -1 , followed by that of Catla catla, Cirrhina mrigala, Ctenopharyngodon idella and Labeo rohita with the mean lethal concentrations of 101.70±16.70, 105.80±12.80, 122.40±23.77 and 128.80±19.95mgL -1 , respectively. Amongst five fish species, Labeo rohita showed significantly least sensitivity towards Zn+Pb mixture (170.00±0.60mgL -1 ) while Hypophthalmichthys molitrix showed significantly higher sensitivity to Pb+Mn with the mean lethal concentrations of 18.53±0.61mgL -1 . The overall sensitivity of five fish species varied significantly for 19 mixtures with the mean higher and lower lethal concentrations of 134.70±26.11 and 73.56±14.18mgL -1 for Zn+Pb and Fe+Zn+Pb+Ni+Mn mixtures, respectively. The exposure of four (Fe+Zn+Pb+Mn) and five (Fe+Zn+Pb+Ni+Mn) metal mixtures, at sub-lethal concentrations (1/3 rd of LC 50 ), caused significantly lesser growth to all the five fish species. Amongst 19 mixtures, Fe+Zn+Pb+Ni mixture (#17) caused significantly pronounced impacts on the growth performance of all the five fish species, followed by thatof Fe+Zn+Pb+Ni+Mn (#19) and Fe+Zn+Pb+Mn (#18) mixtures. Under chronic stress, Cirrhina mrigala and Hypophthalmichthys molitix attained significantly higher weights, followed by that of Catla catla, Labeo rohita and Ctenopharyngodon idella. However, the growth of all the five metals mixture exposed fish species was significantly lesser than that of control fish (un-stressed). Significantly variable condition factor values reflected the degree of fish well-beings that correlated directly with fish growth. The metallic ion loads (under the exposure of different mixtures) of the culture media correlated inversely with fish growth due to significant effects of metal’s stress on fish body. The growth performance of fish did not vary significantly due to change in feed intake while significantly positive change in the feed conversion efficiency had affected the fish growth significantly due to the impacts of various mixtures in reducing fish metabolism and activity. Any significant change in feed intake, due to stress, is reflected in terms of fish growth showing the impacts of various mixtures on fish growth were either additive or antagonist / synergistic. Physico-chemistry of the test media (water) used for different treatments exerted significant impacts on fish growth, feed intake, and condition factor and feed conversion efficiency of fish also. Significantly better feed intake enhanced the ammonia production and excretion by the fish resulting into non-significantly positive relationship of fish weight increments with ammonia contents of the test media. Significantly higher feed intake resulted in excessive excretion of ammonia by the fish to cause significant impact on its growth. Sodium and potassium showed significantlypositive correlation with ammonia concentrations of the test media, indicating excessive release of sodium and potassium by the fish under stress of various mixtures that resulted in significantly more excretion of ammonia by the fish. Organ-wise distribution of residual metals viz. iron, zinc, lead, nickel and manganese reveals liver as the prime site of their accumulation with significantly higher persistence, followed by kidney, gills and fins of metals mixture exposed fish. The fish muscle tissues accumulated significantly (P<0.05) lower metals than liver, kidney and gills. The exposure of metals mixture at higher concentrations resulted in escalated levels of these metals in fish body that followed the general order: zinc >iron >nickel >lead >manganese which can be regarded as an indicator of cumulative response of five fish species. Accumulation of all the metals in fish body followed the general order: liver>kidney>gills>fins>muscle>skin>bones. The level of various metals in gills of fish, exposed to different sub-lethal concentrations of mixtures, was significantly higher (P<0.05) than the levels found in all other organs except liver and kidney.