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Home > Sequencing and Analysis of a Cdna Encoding a Putative Coclaurine N-Methyltransferase Cnmt from Aristolochia Fimbriata, a Basal Angiosperm

Sequencing and Analysis of a Cdna Encoding a Putative Coclaurine N-Methyltransferase Cnmt from Aristolochia Fimbriata, a Basal Angiosperm

Thesis Info

Access Option

External Link

Author

Roshan Ali

Program

PhD

Institute

University of Peshawar

City

Peshawar

Province

KPK

Country

Pakistan

Thesis Completing Year

2010

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1342

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727160791

Similar


Alkaloids are produced in plants through various pathways involving several enzymes that lead to diverse alkaloids. One of the most important alkaloid biosynthetic enzymes is coclaurine N-methyltransferase (CNMT) which is an S-adenosyl-L- methionine-dependent methyltransferase (SAM-MTase). SAM-MTases utilize S- adenosyl-L-methionine (SAM) as a cofactor to methylate other molecules. CNMT catalyzes the methylation of coclaurine. Crystal structures of more than hundred SAM-MTases have been investigated. Several O-methyltransferases have been characterized at the molecular as well as structural levels, but there have been very few molecular studies of N-methyltransferases especially about CNMTs. In this study, the amino acids sequence of Aristolochia fimbriata putative CNMT has been determined by isolating and translating the full-length cDNA. In order to investigate the mechanism of methylation by this putative CNMT, three-dimensional homology model has been built and the ligand (SAM) as well as the substrate (S- Coclaurine) has been docked into its active site. Phylogenetic analyses were performed using the MEGA 4.0 software. The phylogenetic relationship of A. fimbriata putative CNMT with their homologs has also been analyzed. In order to identify the putative CNMT gene and determine its function, online similarity searches were performed by BLAST program using the cDNA sequence as well as the putative protein that could be encoded by the gene. All the methods, applied, predicted that the gene identified might be involved in the production of CNMT. The predicted homology model consists of two domains: the N-terminal catalytic core domain and the C-terminal domain. The catalytic core domain has a central sheet of β-strands surrounded by α helices. The catalytic core domain contains binding site for VSAM. The C-terminal domain consists of alpha helices and a few beta sheets creating a pocket for the substrate in between them. The SAM-binding pocket is located next to substrate binding pocket and there is an opening in between these two cavities through which the methyl group of SAM projects towards the substrate. The most important residues involved in the methyl transfer reaction seem to be Tyr-79 and Glu-96.
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مولانا محمد سلیم

مولانا محمد سلیم
سخت افسوس ہے کہ مدرسہ صولتیہ مکہ مکرمہ کے ناظم اعلیٰ اورمہتمم مولانا محمد سلیم صاحب ایک ماہ کی معمولی علالت کے بعد۱۸/جولائی کوبروزشنبہ نمازفجر کے وقت نصف گھنٹہ پہلے رہ گزائے عالم جاودانی ہوگئے۔ اناﷲ وانا الیہ راجعون۔
مولانا کاآبائی وطن کیرانہ ضلع مظفر نگر تھا۔ آپ کے والد ماجد محمد سعید صاحب المتوفی ۱۳۵۷ھ مولانا رحمت اﷲ صاحب کیرانوی المتوفی ۱۳۰۸ھ صاحب ’’اظہار الحق‘‘کے بھتیجے محمد صدیق صاحب کے فرزند ارجمند تھے۔ مولانا رحمت اﷲ صاحب جب ۱۸۵۷ء کی جنگ آزادی میں شرکت کے بعد مکہ مکرمہ ہجرت کرگئے توآپ نے مولانا محمد سعید کوجن کی عمر صرف بارہ برس کی تھی، اپنے پاس مکہ مکرمہ بلالیا اوران کی تعلیم وتربیت کاخاص اہتمام کیا اوروہ بھی مستقلاً یہیں رہ پڑے، چنانچہ مولانا محمدسلیم صاحب کی پیدائش بھی مکہ مکرمہ میں ہوئی۔ حضرت مولانا رحمت اﷲ نے کلکتہ کی ایک مخیرخاتون صولت النساء بیگم کی امداد واعانت سے مکہ کے محلہ خندریسہ میں زمین کا ایک پلاٹ خریدکرایک عمارت بنوائی اور ۱۲۹۰ھ میں انہی خاتون کے نام پر مدرسہ صولتیہ کے نام سے اس عمارت میں ایک مدرسہ جاری کیا تھا۔مولانا محمد سلیم صاحب نے اسی مدرسہ میں تعلیم پائی۔ فراغت کے بعد پندرہ بیس سال اسی مدرسہ میں درس دیا، ۱۳۴۶ھ میں اس کے نائب ناظم اور ۱۳۵۷ھ میں ناظم مقررہوئے جس پر وہ اخیرتک رہے۔
مولانا نے نصف صدی سے زیادہ کایہ مدرسہ کے اہتمام وانتظام کازمانہ جس عالی ہمتی،صبر واستقلال فہم وتدبر اورلیاقت وقابلیت سے گزاراہے، وہ اُن کی زندگی کاسب سے بڑا اور دوسروں کے لیے ایک بڑاسبق آموزکارنامہ ہے۔ چنانچہ اسی اثنا میں جب ملک کے سیاسی حالات بدلے تو آپ کوچند در چند دشواریوں اور مختلف قسم کی آزمائشوں سے سابقہ پیش آیا، لیکن آپ نے بڑی پامردی اور ہمت واستقلال سے ان کا مقابلہ کیا۔ انہی...

دور الإعلام الجديد في تعزيز قضية المصالحة الوطنية: دراسة ميدانية على عينة من الإعلاميين العاملين في قطاع غزة

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