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Home > Serological Characterization and Molecular Diversity of Citrus Tristeza Virus Ctv from Khyber Pakhtunkhwa Kp and Punjab Provinces of Pakistan

Serological Characterization and Molecular Diversity of Citrus Tristeza Virus Ctv from Khyber Pakhtunkhwa Kp and Punjab Provinces of Pakistan

Thesis Info

Access Option

External Link

Author

Kazmi, Syed Zaigham Abbas

Program

PhD

Institute

Pir Mehr Ali Shah Arid Agriculture University

City

Rawalpindi

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Biochemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12230/1/syed%20Zaigham%20abbas%20kazmi%20biochemistry%202019%20arid%20rwp%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727163805

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Citrus tristeza virus (CTV) is a plant pathogenic virus belonging to the genus Closterovirus and family Closteroviridae. It is transmitted by vegetative propagation and by several aphid species. It has been reported that CTV has killed millions of citrus trees worldwide. CTV has previously been reported in Pakistan. A survey was carried out in the year 2014 and 1260 random samples were tested from six districts of Punjab and seven districts of Khyber Pakhtunkhwa (KP). Results from DAS-ELISA revealed incidence of 28.3 percent in Punjab and 30.8 percent in KP. Seventy symptomatic samples from both the provinces were collected and tested through DAS-ELISA. The major coat protein coding gene of CTV from forty-eight ELISA positive samples were amplified, cloned into pGEM®-T Easy vector, sequenced and phylogenetic analysis was carried out. Nucleotide sequence analysis revealed 90-100% similarity within indigenous forty-eight isolates, 91.1 to 100 percent similarity with six isolates previously reported from Pakistan and 97.7 to 99.7 percent similarity with T3 USA, VT USA, VT Israel, RB New Zealand, VT India and an Indian isolate of unknown genotype. A maximum likelihood phylogenetic tree indicated that CTV population is diverse in Pakistan with different isolates consisting of one major isolate, T3, and three minor isolates, VT, RB, and VT IND. The most common group (T3 like) is comprised of 42 Pakistani isolates including 36 of our samples and is dispersed all over the country irrespective of the region and province. The remaining three groups are related to VT Israel, VT India and RB New Zealand confined to specific regions. One coat protein gene sequence from the major clade were selected and expressed in E. coli Expression system. The expressed protein was purified and used to raise antibodies in rabbits. Our own produced antisera was tested by DAC-ELISA with the infected and healthy plant tissues. The ELISA readings showed positive results with the infected tissues from Pakistan and USA and negative results with the healthy tissues which confirmed the authenticity of antisera. Results indicated that our prepared antisera is more specific and sensitive against Pakistani CTV isolates as compare to commercially available kit. ELISA readings showed difference in reactivity between the antiserum from two rabbits. No difference in the ELISA readings were observed among five bleeds at the same dilution factor which revealed no difference in the antibody titer among the bleeds. Antisera was also tested against four different dilutions of plant extracts and results showed the sensitivity of one antiserum up to 1/100 of the plant extract. We can use the antisera for the indexing of trees in Pakistan and certification schemes could be introduced to reduce the spread of the virus in the country.
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میرا ملک پاکستان

میرا ملک پاکستان
نحمدہ ونصلی علی رسولہ الکریم امّا بعد فاعوذ بااللہ من الشیطن الرجیم
بسم اللہ الرحمن الرحیم
معزز اسا تذہ کرام اور میرے ہم مکتب ساتھیو!
آج مجھے جس موضوع پر اظہار خیال کرنا ہے وہ ہے:’’میرا ملک پاکستان‘‘
صدرِذی وقار!
جہاں تک میرے ملک کا تعلق ہے تو یہ مجھے جان سے بھی زیادہ عزیز ہے، اس کے کھیت وکھلیان عزیز ہیں، اس کے کوہ ودمن عزیز ہیں، اس کے گلستان و چمن عزیز ہیں ، میرا ملک پاکستان ایک ایسی سرزمین ہے کہ جس پر جملہ عالم اسلام ناز کرتا ہے۔ جس کا وجود ہرمسلم کے لیے ناگزیر ہے۔ جس کی بقاء وسا لمیت پرکلمہ گو کا مقصد حیات ہے۔
محترم سامعین !
پاکستان کا قیام ایک نعمت غیر مترقبہ ہے، ہم نے یہ آسانی سے حاصل نہیں کیا، اس میں لاکھوں شہیدوں کا لہو شامل ہے، اس شجر ثمر دار کی بنیادوں میں کئی یتیموں کی یتیمی بھی شامل ہے۔ کئی بیواؤں کے اشکبار آنسوشامل ہیں ، کئی نئی نویلی دلہنوں کی شبہائے عروس شامل ہیں۔ کئی نوجوانوں کی اُٹھتی ہوئی جوانیاں شامل ہیں۔
محترم صدر!
پاکستان ہم نے اس لیے حاصل کیا تھا کہ ہم اپنی عبادت آزادانہ کریں گے، ہم آزادی کی نعمت سے مالا مال ہوں گے۔ ہمیں رکوع وسجود میں آزادی ہوگی ، ہمیںصدقہ و خیرات کرنے میں آزادی ہوگی ہمیں تعلیم کے حصول میں آزادی ہوگی، ہمیں خدمت خلق میں آزادی ہوگی ،ہمیں تبلیغ دین میں آزادی ہوگی، ہمیں تفہیم قرآن میں آزادی ہوگی، ہم جملہ امورِ حیات سرانجام دینے میں آزاد ہوں گے۔
صدرِ ذی وقار!
ہم قائد اعظم محمدعلی جناح اور سلف صالحین کی کوششوں سے آزاد تو ہو گئے لیکن ہم نے آزادی کا مطلب غلط سمجھ لیا، ہم مادر پدر آزاد ہو گئے ، ہم اقرباء پروری...

اسلام کے عبوری قوانین

Being the natural religion, Islam demands peaceful, liberal and civilized society. To achieve this goal Islam introduces two types of laws; permanent and Transitional law. Permanent law are those which are abide by every person of the Muslim society i.e Marriage laws, economic laws, political laws, Family laws, heir ship laws, social laws and other such laws. On the other hand there are some temporary or Transitional laws; i.e War laws, Divorce laws, laws for Slave or laws to end slavery, Criminal Laws (Qis┐s, Diyat, Rajam and Lashes etc), there laws are put into practice only in conditional situations. After achieving the goal these Transitional laws are no more in practice. Islam is complete code of life and gives Laws and Rules for any situation. Islam is natural and liberal religion, its laws since beginning are very much practicable and result oriented. Whenever and where ever Islamic laws have been put into practice society has got its fruits. In this article Islamic Transitional Law are briefly discussed to understand the background of transitional laws. Detail of these laws can be seen in Hadith and Fiqah books.

Production Potential of Olive Cultivars at Different Maturity Stages and Optimization of Propagtion Techniques

Production potential of olive cultivars (Frontoio, Manzanilla, Ottobratica, Pendolino and Picual) at different maturity stages (Lemon green, Semi-ripe and Ripe) was investigated and propagation techniques through cutting and air-layering were optimized during 2014 and 2015 under irrigation condition at Olive Model Farm Sangbhatti, Mardan-Pakistan. Flowering date was taken as base line for phonological attributes. Cultivar Pendolino started early flowers opening on 12th April and took more days (9.50) upto fruit set, while less days (6.33), started from 18th April were noted for Ottobratica. Manzanilla and Picual attained lemon green maturity for harvesting after 193.50 (25th October) and 192 (22nd October) days respectively, while 202.17 (2nd November) and 201.33 (1st November) days were taken by these cultivars to reach semi ripe stage and 214.17 and 210 days to ripe stage of harvesting respectively. Frontoio, Ottobratica and Pendolino attained semi ripe stage after 180.17 (11th October), 184.50 (20th October) and 193 (22nd October) days respectively, while 188 (19th October), 195 (30th October) and 203.67 (2nd November) days were taken by these cultivars to attain ripe stage of harvesting. The cultivar Manzanilla produced heavy (4.34 g) fruits; however large sized (4.48 cm3) fruits having more pulp:stone (4.94) were yielded by Picual. The oil extracted from Manzanilla cultivar has less peroxide value (3.21 meq kg-1), more Phenol (616.67 mg kg-1) and chlorophyll (4.58 mg L-1). High fruit yield (35.81 kg tree-1) with more oil percentage (14.66%), less free fatty acid (0.43%) and more carotene (2.70 mg L-1) were determined in the oil extracted from fruits of Frontoio. The values of the quality attributes were within the range of IOC trade standards (IOC, 2003) of extra virgin olive oil category, with the exception of free fatty acid (%) recorded in the oil extracted from fruits of Ottobratica, harvested at ripe stage of maturity that fulfilled the requirement of virgin category. Yield and yield components enhanced from lemon green to semi-ripe and ripe stage of harvesting. Free fatty acid (0.25%) and peroxide value (3.04 meq kg-1) were lower and total phenols were higher (530 mg kg-1) in the oil extracted at semi-ripe stage, while high free fatty acid (1.50%), peroxide value (4.43 meq kg-1) and low phenol (361.67 mg kg-1) were determined in the oil extracted from fruits harvested at ripe stage. The chlorophyll and carotenoids contents significantly decreased with fruit ripening ranged from 4.99 to 2.41 mg L-1 and 3.17 to 1.49 mg L-1 respectively from lemon green to ripe stages of harvesting. The multiplication of olive cultivars (Frontoio, Manzanilla, Ottobratica, Pendolino and Picual) through cuttings and air-layering was optimized in sequential experiments. Semi hardwood cutting proved to be the root generative type. The cuttings of Frontoio produced highest number of roots (10.56) and root length (6.61cm), followed by the cuttings of cultivar Manzanilla that generated 8.17 numbers of roots with 6.06 cm length, while less number of roots (5.50) was produced by cuttings taken from cultivar Pendolino. The sapling of Ottobratica produced through cuttings accomplished 23.64% survival, followed by survival percentage (19.48 and 15.7%) noted in the saplings of cultivar Manzanilla and Frontoio respectively, while less survival percentage (9.73%) recorded in saplings of Pendolino. The semi hardwood cuttings of olive cultivars treated with 3000 ppm IBA solution enhanced rooting. The cuttings of Frontoio produced maximum number of roots (14.50), root length and weight (8.12 cm and 5.53 g respectively), followed by cuttings of Manzanilla which generated 12.04 number of roots with 7.19 cm length while less number of roots (8.57), root length and thickness (4.33 cm and 3.94 mm, respectively) were produced by Pendolino. Manzanilla attained 43.05% survival followed by Frontoio with 40.38 % survival, while less survival (23.24 %) was recorded in plants of Pendolino. The time interval of mid August proved to be most favorable time for air-layering in terms of rooting percentage (42.40 %), number of roots (4.37), root length (4.35 cm), weight (1.68 g), number of re-sprout (3.67) and shoot length (5.61 cm) while late and early layering responded poorly to all the studied attributes. The layering treated with 2000 ppm took less days (35.13) to root appearance, accomplished 72.00% rooting success, generated 5.85 number of roots with 6.65 cm length and 2.47 g weight and 4.89 number of re-sprout and 7.00 cm shoot length. The untreated layering took maximum days to root appearance (50.40), attained less rooting percentage (47.07%). The layering of Manzanilla generated early rooting and took less (36.00) days for root appearance, accomplished high rooting percentage (77.47 %), produced more (6.38 number) and lengthy (7.03 cm) roots and more (5.57) re-sprouts, followed by layers of Frontoio that took 38.8 days for root appearance, attained 65.73% rooting success and produced 5.69 number of roots with 6.14 cm length while the layers of Pendolino took maximum days for root appearance (48.40) and accomplished 48.27% rooting. It is concluded that the olive cultivars Frontoio, Manzanilla and Picual were found high yielding with good oil recovery of extra virgin oil grade. Pendolino is marginally accepted, while the performance of Ottobratica was not satisfactory under the agro-climatic conditions of Sangbhatti, Mardan. The olive fruits harvested at semi ripe stage provided equilibrium in yield, oil recovery and quality attributes. The semi hard wood cuttings treated with 3000 ppm IBA solution developed profuse root and shoot systems in olives cultivars. The plants of Frontoio, Manzanilla and Pendolino when air-layered during mid-August, treated with 2000 ppm IBA solution attained high rotting percentage. Ottobratica produced maximum number of roots when air-layered during mid July, treated with 1000 ppm IBA and Picual during late July, treated with 2000 ppm IBA solution.