Brucellosis is one of the world’s major zoonotic problems. It may cause considerable economic losses. In livestock, it results in reduced productivity, abortion, weak offsprings and is a major impediment for the trade. Almost all domestic species can be affected by this disease. Aim of the present study was to know the prevalence of brucellosis in different food animals and humans. For this purpose, 2375 serum samples were collected. Rose Bengal Plate Test (RBPT) and Serum Agglutination Test (SAT) was applied for initial screening and then positive samples were subjected to i-ELISA and c- ELISA for conformation. Experimental infection was induced in rabbits to study the histopathological changes in different organs. The data thus collected was interpreted and subjected to statistical analysis. The sero-prevalence of brucellosis in relation to age, sex, parity and stage of lactation of the animal was determined.