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Serum Biomarkers Profiling for Hepatocellular Carcinoma Patients Positive for Chronic Hepatitis B and C Infections

Thesis Info

Access Option

External Link

Author

Fahim, Amin

Program

PhD

Institute

Isra University

City

Hyderabad

Province

Sindh

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/9110/1/Full%20Thesis.pdf

Added

2021-02-17 19:49:13

Modified

2023-01-06 19:20:37

ARI ID

1676727167613

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مولانا محمد سورتی

مولانا محمد سُورتی ؒ
پچھلے مہینہ کا سب سے اندوہناک علمی حادثہ مولانا محمد سورتی کی وفات ہے، مرحوم اس عہد کے مستثنیٰ دل و دماغ اور حافظہ کے صاحب علم تھے، جہاں تک میری اطلاع ہے اس وقت اتنا وسیع النظر، وسیع المطالعہ، کثیر الحافظہ عالم موجود نہیں، صرف و نحو، لغت و ادب و اخبار و انساب و رجال کے اس زمانہ میں در حقیقت وہ امام تھے، وہ چند ماہ سے مرض استسقا میں مبتلا تھے، علی گڑھ میں ان دنوں قیام تھا اور وہیں ۷؍ اگست کو بروز جمعہ وفات پائی۔
مرحوم کا اصلی وطن سورت (گجرات) تھا، وطن میں ابتدائی تعلیم پاکر یہ دلّی آئے اور رامپور میں مولانا محمد طیب صاحب مکی کا تلمذ حاصل کیا، میری ان کی پہلی ملاقات ۱۹۰۸؁ء میں ہوئی، جب مولانا طیب مکی رامپور چھوڑ کر دارالعلوم ندوہ لکھنؤ میں ادیب اول کے عہدہ پر فائز تھے، فاضل استاد کے ساتھ یہ لائق شاگرد بھی لکھنؤ وارد ہوا اور اس زمانہ سے لے کر اخیر تک ان کے ساتھ میری علمی رفاقت اور ذاتی دوستی کا سلسلہ قائم رہا، معارف بھی ان کے رشحات قلم سے کبھی کبھی مستفید ہوتا رہا ہے۔
مرحوم اس فضل و کمال کے باوجود ہمیشہ پریشان حال رہے اور کہیں ایک جگہ جم کر بیٹھنا ان کو نصیب نہ ہوا، اس کا نتیجہ یہ ہوا کہ ان کے علم سے استفادہ بہت کم کیا جاسکا اور کوئی کارآمد تصنیف بھی اپنی یادگار نہ چھوڑ سکے اور نہ کوئی لائق شاگرد ہی ان کا قائم مقام ہوسکا، البتہ چند جسمانی اولاد ان کی یادگار ہیں۔
ایک زمانہ میں جامعہ ملیہ دہلی میں معلم رہے، پھر بنارس کے جامعہ رحمانیہ میں مدرس ہوئے، بعد کو بمبئی میں ایک اہلحدیث مدرسہ میں حدیث کا درس دینے لگے تھے، ٹونک کے مشہور کتب خانہ کی...

China-Pakistan Economic Corridor: A Case Study Of Internal Security Challenge Faced by Pakistan

The strongest and most crucial strategic and economic links in the south Asian region are the friendships between Pakistan and China. China and Pakistan assert that the people who live on either side of the Himalayas have interacted with one another in a trustworthy and sincere manner from ancient times. Chinese investment in the CPEC represents the greatest sum (62 billion dollars) ever between two friendly nations. China and Pakistan are dealing with numerous internal and external security challenges as a result of their strong friendship and significant investment in this project. Both nations, such as India and Afghanistan, are posing security problems to Pakistan, which has several security issues. The USA, India, the UK, and Israel are also causing strife and tension in Pakistan. These nations are also causing internal problems by sponsoring terrorist organizations like the Taliban and ISIS as well as nationalist organizations like the Baloch, the Lashkar-e-Jhangvi, and Sipah Muhammad. The challenges to Pakistan's internal security are discussed in this study.

Rna Interference Based Therapeutic Interventions for Leukemias

Leukemia, a heterogeneous group of hematological malignancies, continues to cause significant morbidity and mortality despite decades of research and development. Chromosomal aberrations are the main cause of leukemia and lead to the generation of fusion/chimeric genes, resulting in activation of proto-oncogenes and suppression of tumor-suppressor genes. Incidence of different aberrations associated with different leukemias varies in different regions of the world and the data from population based studies in South Asia, including Pakistan, are lacking. The expression of chimeric/fusion genes can be detected using sensitive molecular methods like reverse transcriptase polymerase chain reaction (RT-PCR) and dot blot hybridization. In this study, classic BCR-ABL t(9;22) variants (e13a2 and e14a2) were detected in 96% of CML patients, while one of the patient possessed a unique e13-1a BCR-ABL variant. A total of 68 patients of paediatric ALL, were screened by RT-PCR to determine the relative frequency of t(9;22), t(12;21), t(1;19), and t(4;11,). Translocation (9;22) was seen in 2/68 (3%) and t(1;19) in 2/68 (3) children. Seven children showed t(12;21) while 8 showed t(4;11) translocations. In AML patients, t(8;21) was found in 4/21 patients while t(1;19) was seen in only one of the patient out of 21 screened. Thus, there appears to be a significant under representation of the fusion transcripts for TEL-AML, a good prognostic marker, in this study, unlike in the West, where it is seen in 35% of children with ALL. This, together with the generally increased leukemic burden seen in Pakistani patients, may explain in part, the poor treatment outcome reported. Conventional therapeutic approaches for leukemias include chemotherapy, radiation therapy, interferon therapy, stem-cell transplantation and surgery but their application is limited due to their side effects. The advent of RNA interference (RNAi) technology has opened the door to previously unrefined methods of therapeutic interventions. Gene targeting of the chimeric genes by small interfering RNA is an ideal way to kill tumor cells specifically, while leaving the normal cells unaffected. In case of CML, the over- expressed protein, tyrosine kinase, from BCR-ABL fusion genes, trigger malignant transformation and abnormal proliferation of the cells. Although targeting the BCR-ABL tyrosine kinase activity by imatinib mesylate has rapidly become first-line therapy, drug resistance suggests that combination therapy directed to a complementing target may significantly improve treatment results. To identify such potential targets, we used lentivirus-mediated RNA interference (RNAi) as a tool for functional genomics in human leukemic as well as murine hematopoietic cell lines. Expression of STAT1 and STAT3 proteins was successfully knocked down using specific shRNAs targeting STAT1 and STAT3 mRNA. RNAi-mediated reduction of STAT1 and STAT3 protein expression inhibited BCR-ABL–dependent (K562) but not cytokine-dependent (32D) cell proliferation. The data indicate that BCR-ABL expression may affect the function of normal signaling molecules. Targeting these molecules may harbor significant therapeutic potential for the treatment of patients with CML.