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Home > Studies on Amylases from Locally Isolated Strains of Aspergillus and Their Characterization

Studies on Amylases from Locally Isolated Strains of Aspergillus and Their Characterization

Thesis Info

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Author

Yahya, Saira

Program

PhD

Institute

University of Karachi

City

Karachi

Province

Sindh

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Microbiology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12900/1/Saira_Yahya_Microbiology_HSR_2018_UoK_Karachi_01.11.2018.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727273033

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Microbial amylases are as important in industrial processes as are proteases. Among the microbes, fungi are gaining repute for the production of amylases. Keeping this in view, the present study was carried out to isolate, identify, characterize and explore the biotechnological applications of indigenous fungal strains. The study began by reviving fungal cultures from the stock collection in our lab and six more fungi were further isolated from the contaminated starch-agar plates. The isolates identified on the basis of cultural and morphological characteristics belonged to genus Aspergillus, Penicillium and Rhizopus. Preliminary screening was performed on starch-agar plate method with minor modification. Amylase production from the fungal isolates was also carried out under submerged fermentation conditions using mineral-salt media supplemented with starch and amylase production was quantitatively evaluated. Based on the results for quantitative production of amylases, 4 fungal isolates showing high IU/ml of amylase productivity were selected for further studies. The amylases from these isolates were characterized on the basis of activities at high temperatures and 2 fungal strains A. tubingensis SY 1 and A. niger MS 101 showing activities at 60oC and 64oC, respectively, were selected. Afterwards, the conditions for the optimum production of amylases from A. tubingensis SY 1 and A. niger MS 101 were worked out. The fungal strains showed optimum amylase production at 30oC with an initial pH of 5.9. Among the carbon sources; starch, glucose and maltose displayed higher amylase production along with the organic nitrogen source peptone. Amylase production was also optimized using a Plackett-Burman statistical design, and the results revealed peptone as the superior factor responsible for higher amylase titers. The optimum pH for amylase activity was determined along with the determination of optimum substrate concentration, the effect of various metal-ions and enzyme modulators. The pH 5.6 was optimum for amylase activity from both the fungal strains, while starch concentration of 0.5% was found to be optimum for the enzyme-substrate reaction to be carried out. Mn2+, K+ and NH4+ ions enhanced amylase activities while urea crystals and EDTA slightly inhibited the amylase activities of both fungal strains. Studies on solid-state fermentation (SSF) and submerged fermentation (SmF) for amylase production was also performed using variety of natural substrates including 2 halophytic plants and the results were compared. Whenever studies were compared with crude natural carbon substrates, whether under solid-state or submerged fermentation conditions together with the quantitative determination of amylase, the concentration of other enzymes, like xylanase, pectinase and cellulose enzyme system (β-glucosidase, endoglucanase, filter paper assay) were also determined. Potato-peels were found to be the most suitable substrate for amylase production by both fungal strains under SmF and SSF conditions. The Tm of amylase from the strain MS101 of A. niger was 65oC and from A. tubingensis SY 1 was 67oC, while Ea values were 73.64 KJ/mol and 46.07 KJ/mol for A. niger MS101 and A. tubingensis SY 1 amylases, respectively. Because of higher Tm values and low energies of activation (Ea) the industrial potential of amylases was determined. For this purpose, the starch-sized fabric was treated with fungal amylases at different temperatures for different time intervals to determine the d-sizing efficiency of amylases. The fabric after de-sizing by A. niger MS 101 amylase resulted in a TEGEWA rating of 8, while by A. tubingensis SY 1 amylase a TEGEWA rating of 9 was observed at 54oC in 12 hr. The results are promising for the use of these amylases in de-sizing. Co-culture studies for bioethanol production under SmF and SSF conditions were carried out using potato-peels under SmF and SSF, when the fermentation medium was simultaneously inoculated with the fungal and yeast strains, ~4 g/Kg and 6 g/Kg ethanol was produced in 120 hr. of incubation at 30oC. The yeast Pichia kudriavzevii SY 11 was also able to produce almost similar amount of ethanol under SmF of potatopeels. Indicating no contribution of fungal amylase to bioethanol. However, when coculture studies were carried out on purified starch 7- to 12- fold more ethanol production was noted (12 and 28 g/Kg) compared to potato-peel (1 and 4 g/Kg). Amylases were subjected to purification using different techniques: affinity and gelfiltration chromatography. No fruitful results were obtained by affinity chromatography while by using gel-filtration technique; a band of ~116 kDa was observed for A. tubingensis SY 1 amylase.
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جالب میں اور کوٹ لکھپت جیل

جالب میں اور کوٹ لکھپت جیل

                                                                                                                ڈاکٹر اسرار شاہ

لاہور میں دوست جالب میلے کا انعقاد کر رہے ہیں اور میں کاغذ اور قلم پکڑے اپنے ماضی میں کھو گیا دوستوں نے اصرار کیا کہ اسرار شاہ لکھو۔

میری دعا ہے کہ کوئی نیا ضیاء الحق پیدا نہ ہو اور مجھے عمرِ رفتہ میں لے جائے میںکالج سے نکلوں تو ایسی یونیورسٹی میں داخل ہو جائوں جہاں ڈاکٹر مبشر حسین ،میاں محمود علی قصوری رائو رشید،رضا کاظم ایڈووکیٹ چوہدری اعتزاز حسین ،جسٹس سعید حسن ،آئی اے رحمن ،پروفیسر امین مغل،چوہدری اصغر خادم ،رشید قریشی ،شعیب ہاشمی ،حمید اختر ،محمد علی ایکٹر اور حبیب جالبؔجیسے پروفیسر اور اساتذہ نظر بند ہوں نئی نسل نا واقف ہے کہ یہ تمام لوگ اپنی ذات میں ایک ادارہ تھے اور ان میں کچھ آج بھی حیات ہیں ۔

کوٹ لکھپت جیل بھی کیا جیل تھی ،جیل کے سپرنٹنڈنٹ نے جیل کی دیوار کے ساتھ شام کو واک کر نے کی اجازت دی ڈاکٹر مبشر صاحب جیل میں ’’ماں ‘‘کا کردارادا کر رہے تھے وہ جیل سے راشن لیتے اس کو پکواتے تمام لوگ چٹانوں پر بیٹھتے اور سب میں برابر تقسیم کرتے ۔صبح دس بجے سے لے کر دوپہرکے کھانے تک عبدا ﷲملک صاحب کے کمرے میں سٹڈی سرکل ہو تا اور آئی اے رحمن صاحب لیکچر دیتے اور تمام سر نگوں ہوتے ۔

حاجی رشید انور جن کا تعلق مزدور کسان پارٹی سے تھا کیا خوبصورت انسان تھے عمر کے اعتبار سے وہ میرے والد کی طرح تھے جسم میں سی آئی اے چونا منڈی کے تششدد کی دردیں موجود تھیں وہ صبح میرے جسم کو دباتے اور بچوں کی طرح پکارتے ہوئے اٹھاتے کہ ’’اسرار شاہ ‘‘اٹھ جائو سورج نکل آ یا...

برصغیر میں محدثین کی خدمات حدیث: تاریخی و تجزیاتی جائزہ

After the Prophet r the Muslims all over the world associate themselves with him by following his pious deeds and acting upon his sayings (Hadith). The Muslims of the sub-continent have been very zealous in this respect and have done great job in this regard. In the subcontinent, the sayings of the Holy Prophet reached with Islam during the era of pious caliphs. In those days, according to some traditions, 25 companions of the Holy Prophet ﷺ Sahaba (R. A) and 42 Tabe-ien (those who had seen the Sahaba R. A i-e their successors) came to India and preached Islam. This preaching was continued by later Muslims and the rulers like Mohammad Bin Qasim and Mehmood Ghaznavi. The services of great Muhaddeseen (narrators and illustrators of the sayings of the Holy Prophet r like Musa Bin Yaqoob, Yazid Bin Abi Kabsha, Abu Musa Israeel Bin Musa and Abu Hafs Rabi Bin Sabih are note worthy. They provided local people the knowledge of Hadith. These scholars earned fame and prestige by their great works in this field. Shah Waliuallah wrote Mussffa and Maswwa, in subcontinent there are great many institutions like Jamia Salfiya Faisalabad, Jamia Ashrafia Lahore, Jamia Naeemia Lahore, Khair- ul-Madaras Multan, Jamia Mohammadia Gujranwala, Dar-ul-Hadith Delhi and Jamia Salfiya Banaras to teach the knowledge of Hadith.

قراءات الإمام عبدالله بن عباس-رضي الله عنهما- وأثرها في تفسير كتاب الله جمع و دراسۃ وتحلیل

Qira’at is plural of Qiraatun which literally means the reading, terminologically means the method of pronunciation used in the recitation of Quran. These methods are different from the seven forms or modes (ahruf) in which the Quran was revealed. The various methods have all been traced back to the Prophet through a number of Sahaabah (R.A) who were most noted for their Quranic recitations. These Sahaabah (R.A) recited the Quran to the Prophet or in his presence and received his approval. Ibn ''Abbaas (R.A), is called the master commentator of the Quran among the Sahaabah. He was one of the renowned Moffassir of Quraan. He has a prominent and unique position among all the Moffassireen. His interpretations of Quraanic verses and Qiraat have a great impact on all most all Tafasir ul Quraan.. In spite of all this significance, the work of Ibne Abbaas (R.A) is scattered in various books of Athar and Tafathir and no effort has ever done to compile his Qiraat, their impact on science of interpretation of Quran in a separate volume and no research has done on their Asanid . This study aims to answer the following questions: 1) Whether the Qiraat of Ibne Abbas (R.A) are so frequent in numbers and have they made major impact over Tafsir ul Quraan, that research can be carried out in this regard? 2) What is the position of Qiraat of Ibne Abbas (R.A) according to their Asanid? 3) What is the significance of Qiraat of Ibne Abbbas (R.A) as for as science of interpretation of Quraan and science of Qiraat is concerned? 4) Whether is it possible to over look the Qiraat of Ibne Abbas (R.A) in the study of Tafsir ul Quran? Research Methodology The research methodology will include collection, study, and assessment of Qiraat of Ibn Abbas (R.A), The research methodology will be systematic analysis. It will include the collection of Qiraat of Ibn Abbas which are scattered in numerous islamic books i.e. Al-Bahrul Moheet, Tafsir ibne kathir, Al-Hujjah fi Qiraat-e-saba,Sahiul Bukhari, Sahihul Muslim, Munjidul Muqreen, Al-Mabsood, Maani-ul-Qiraat, AlMuhtasib etc. The assessment of their Asaneed will also be carried out. Their impact on the interpretation of holy Quraan will be done. His impact on famous Aaima e Qiraat, who borrowed a lot from him, will be discussed. More so, his point of difference with other Aima of Qiraat will also be analyzed. In short, this research would be inductive, comparative, and analytical in nature.