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Studies on Bacteriocins from Environmental Bacteria

Thesis Info

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Author

Syed Iqbal Alam

Program

PhD

Institute

Federal Urdu University of Arts, Science and Technology

City

Karachi

Province

Sindh

Country

Pakistan

Thesis Completing Year

2010

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/707

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727275142

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The present study is primarily concerned with bacteriocins from environmental bacteria. Different bacterial strains were isolated from various environments including hospital air, soil and sewage etc. A total of 51 isolates were screened for bacteriocinogeny, of which 41% (21 isolates) were exhibiting bacteriocinogeny. Two isolates, one of Pseudomonas sp. 40 and Bacillus subtilis strain15 were selected for further studies. Antimicrobial substance from Pseudomonas sp. 40 was neither sensitive to proteolytic enzymes nor showed any antibacterial activity on SDS-PAGE indicating its possible non-proteinaceous nature and hence further studies were not carried out on this strain. Only Bacillus subtilis stain 15 was further studied as it was producing some antimicrobial substance of protein in nature. BLIS of Bacillus subtilis strain 15 was produced during the late exponential-phase or at the beginning of stationary-phase. The inhibitory substance/BLIS was partially purified by ammonium sulphate precipitation. The antimicrobial substance was characterized as a bacteriocins-like inhibitory substance with a molecular weight ranged between 3-5 kDa as determined by SDS-PAGE. BLIS showed the inhibitory activity against locally isolated Bacillus species as well as Bacillus subtilis ATCC 6633 and Bacillus cereus ATCC 11778, Listeria monocytogenes, Staphylococcus aureus, Staphylococcus epidermidis and also showed slight activity against Salmonella typhi and Shigella dysenteriae and those involved in the food-poisoning and spoilage such as Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Micrococcus luteus were inhibited that indicates its possible application in food preservation. BLIS acted bacteriostatically rather than bacteriocidally, on the sensitive cells. It may exhert bactericidal action at higher concentrations. BLIS under study was heat stable up to 80°C as its activity was not affected at this temperature. The BLIS retained its activity over a wide range of pH i.e., from 3-9. It was sensitive to proteinase-K, and α-chymotrypsin indicating about its nature as protein. Its activity remained unaffected in presence of organic solvents like acetone, chloroform, methanol and EDTA except ethyl-alcohol. BLIS from Bacillus subtilis strain 15 showed the effective inhibition of superoxide production in both neutrophils and macrophages. It also had inhibitory effect on whole-blood. The inhibition of superoxide production shows that BLIS may have anti- inflammatory or immuno-suppressive effect. In addition to immunomodulatory activity, BLIS from Bacillus subtilis strain 15 had moderate anti-oxidant activity indicating its probable role in anti-tumorogenic activity.
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عبدالسلام سلام ؔسندیلوی

ڈاکٹر عبدالسلام سلامؔ سندیلوی
افسوس ہے ۱۶؍ ستمبر کی شب میں معروف محقق و نقاد اور شاعر ڈاکٹر عبدالسلام سلامؔ سندیلوی کا انتقال ہوگیا، وہ ہردوئی ضلع کے مردم خیز قصبہ سندیلہ میں ۲۵؍ فروری ۱۹۱۹؁ء کو پیدا ہوئے تھے، لکھنؤ میں اعلیٰ تعلیم حاصل کی، ان کو اردو فارسی کے علاوہ ہندی اور سنسکرت پر بھی عبور تھا، اردو میں پی۔ایچ۔ڈی اور ڈی۔لٹ کی اور ہندی میں وشارد اور ساہتیہ سدھا کر کی ڈگریاں حاصل کیں، مضمون نگاری اور شعر و شاعری کا چسکا طالب علمی کے زمانے میں لگ گیا تھا، شعر و ادب اور تحقیق و تنقید ان کا خاص موضوع تھا، ان میں درجنوں تصنیفات یادگار چھوڑیں۔ اردو رباعیات، اردو شاعری میں نرگیست اور اردو شاعری میں منظر نگاری بڑی اہم تصانیف ہیں، اول الذکر پر پی۔ایچ۔ڈی اور ثانی الذکر پر ڈی۔لٹ کی ڈگری ملی، ۱۹۵۹؁ء میں گورکھ پور یونیورسٹی سے وابستہ ہوئے اور پھر یہیں کے ہوگئے۔ وظیفہ یاب ہونے کے بعد بھی قلم و قرطاس سے تعلق باقی رہا، مگر کئی برس سے بینائی چلی گئی تھی اس لئے معذور اور خانہ نشین ہوگئے تھے، ڈاکٹر صاحب کی تصنیفات کمیت و کیفیت کے لحاظ سے اہم تھیں، لیکن اعزاز اور عہدے کے لئے بھاگ دوڑ ان کو پسند نہ تھی، اس لئے ان کو خاطر خواہ شہرت نہیں ملی، اس سال ان جیسے مستحق کو اترپردیش اردو اکادمی کا مولانا ابوالکلام آزاد ایوارڈ دیا جانا قابل ستایش اقدام تھا، اﷲ تعالیٰ مغفرت فرمائے اور پس ماندگان کو صبر وقرار بخشے۔ آمین!! (ضیاء الدین اصلاحی، اکتوبر ۲۰۰۰ء)

المنهج العاطفي وأهميته في الدعوة إلى الله

The science of Daʿwah has become an independent discipline in the field of Islamic Studies as many researches have discussed its usefulness, historical development, and ground consequences into the society. A preacher draws the sketch of his message by consulting the Quran, Sunnah the is biography prophetic The. (صلى الله عليه وسلم) Prophet Holy of biography and practical example and model in order to preach effectively and call people (صلى الله عليه وسلم) Prophet Holy The. Religion towards particular youth and general in gave a particular emphasize upon the young men of society by giving them peaceful message of Islam and calling towards the light of Islam from darkness of ignorance. In this connection we find many approaches in the life of Holy Prophet (ﷺ) to call youth towards Allah. Among these approaches, one of most common and useful approach is to agitate the emotions and feelings which is also called as emotional approach. In this academic work, the author will discuss the usefulness of this approach by descriptive and qualitative The. (صلى الله عليه وسلم) Prophet Holy of life the into looking research approach has been employed for the collection and analysis of data. The author intended to address the answer of the questions that why is there a gap between the dāʿī and the youth today? Has this led to the failure to keep up with the doctrinal methods of contemporary religious developments and needs? What is the appropriate approach of preaching religion for young people today? Along with the answer of these questions, the author will draw a conclusion and present some recommendations at the end of article.

Pakistan-Us Security Relationship 1947-2006 Analysis of Areas of Convergence and Divergence

The US – Pakistan Security Relationship is almost as old as Pakistan itself. Its origin dates back to the initial days of partition, when a nascent, weak nation, born with a major birth defect “The Kashmir Issue”, looked for external help and support against a hegemonic neighbour. Since then, the US-Pakistan security relationship has been a roller – coaster ride of intimate interaction and a pariah state status during Pakistan’s 63 years existence. The world’s major military and economic power has maintained, what has been termed by many as “transactional” relationship, with its much smaller South Asian ally. During the height of the cold war struggle and the heydays of pacts and alliances, as US looked to form an anti - communist ring around the Soviet Union, Pakistan emerged as a staunch ally in the South – Asia region. It was called by many as the “Most Allied Ally” of the United States. In the ardent quest for gaining military and economic benefits from the US, it invited the wrath of the Soviet Union, hoping to use the US support in resolution of the long – standing dispute with India over Kashmir, and bolster its security against Indian military threat. The seismic changes brought about by the Indo – China War of 1962, radically altered this cozy equation for Pakistan. Its rapprochement with China became a major stumbling block in the security relationship and resulted in estrangement with the US. Pakistan’s military gamble in 1965 to settle the Kashmir dispute resulted in disappointment. Not only did the country fail to achieve anything tangible in Kashmir but the war also exposed all the inconsistencies and differences in the strategic perception of US and Pakistan, ultimately ending the security partnership. Though the alliances continued to exist on paper, yet they did not carry any real weight. President Nixon’s quest for rapprochement with China in 1969-1970 ironically brought Pakistan to the centre stage once again. Now the same issue on China, which had been a source of discord, revitalized the mutual relationship. While General Yahya acted as an intermediary in the US – China dialogues, the country drifted towards anarchy and breakup, owing to years of mismanagement and neglect towards the Eastern Wing. India’s invasion of East Pakistan, resulted in dismemberment of the country and a humiliating defeat, which owed much to the problems of successive regimes in West Pakistan. Yet the US response during crisis once again came under sharp criticism, as it was considered to be Pakistan’s patron and ally, and expected to support the country in the time of need. The thesis tries to identify and analyze the differing strategic perspectives during the two Indo-Pakistan Wars, as well as perceptions that resulted in disappointment and disenchantment in Pakistan. In the aftermath of the 1971 debacle, Prime Minister Bhutto looked towards nuclear deterrence as a security against Indian hegemony, because of failure of the security relationship with US to deliver at the critical time. This led to a new source of divergence in the security relationship, as the US non – proliferation goals clashed with Pakistan’s security imperatives. The Soviet invasion of Afghanistan led to a period of close security relationship, which was terminated abruptly by the US, in the wake of Soviet withdrawal from Afghanistan. This decision reinforced perceptions in Pakistan that US is an unreliable partner. The coming decade was that of Pakistan being put under pressure for its nuclear and missile programs. It also pursued an Afghan policy according to its own national interests that further estranged it from its closest ally, with every passing year. Pakistan’s support to the obscurantist Taliban regime resulted in gradual estrangement of the US Government. Even more sinister for Pakistan’s interests, was the nexus between Taliban and Al-Qaeeda. This was however glossed over by Pakistan, till the fateful events of September 11, 2001. The September 11 attack on the World Trade Center in 2001, was a defining moment in the security relationship, as President Musharraf was asked to make a tough policy decision. This time Pakistan was threatened with military action, if it decided to continue support for the Taliban. Pakistan’s decision to join the war on terrorism has resulted in a renewed security relationship with added economic and political benefits. Yet the tough decisions have resulted in internal discontent and unrest, which Pakistan is still grappling with. Now, as the Obama Administration looks towards an exit strategy from Afghanistan, Pakistan figures out as an essential component of any successful US disengagement. Will the security relationship last after the present partnership of war on terror, or wither away as the historical precedent indicates? What are the ideal building blocks of a stable, sustainable and mutually rewarding partnership? These are the questions which this thesis attempts to answer, in the light of the analysis of area of convergence and divergence over the last 63 years.