Present studies were carried out at Rangeland Research Institute, NARC, Islamabad and Quaid-i-Azam university, Islamabad during 2001-2011. Dry seeds of Sudan grass (Sorghum vulgare var, Sudanese) were subjected to ten acute irradiation treatments ranging from 0 to 45 kR with 5 kR intervals at Nuclear Institute for Food and Agriculture (NIFA), Peshawar. Overall germination percentage decreased with increase in the doses of gamma irradiation. Seedling height decreased significantly (p≤0.05) on 4 th and 11 th day of sowing with increasing treatments of gamma irradiation. Similarly root length and root shoot ratio on 4 th day of sowing under lab conditions also depicted significantly (p≤0.01) decreasing effect with an increase in dose rate of treatments. Data regarding plant height and leaf area showed significantly (p≤0.05) increasing effect with an increase in dose rate of treatments under field condition in M-1 generation. Average tiller plant -1 increased significantly (p≤0.01) with the increase of doses in M-1 generation under field condition. Tillers showed a significantly (p≤0.05) increasing effect with increasing treatments in M- 2 generation. Data regarding green matter weight -1 and dry matter weight -1 showed significantly (p≤0.01) increasing effect with highest dose rate of gamma irradiation in M- 3 generation. Data regarding immature flowering percentage also showed increasing effect significantly (p≤0.05) which is a positive sign to prolong its life span till start of winter season which is a lean period for livestock grazing feed under natural conditions. Overall growth rates improved by gamma irradiation treatments in M-4 generation and M-5 generations. On the basis of these results high yielding mutants in Sudan grass may be developed. Data reading proximate analysis showed insignificant differences in all traits studied in M-1 generation of Sudan grass under field condition. Data regarding effect of irradiation on HCN concentration showed significant (p≤0.01) decreasing effect in red and blue color intensity. While insignificant decreasing effect in green color xiintensity which is a positive sign to reduce HCN concentration in Sudan grass and other sorghum species used as a fodder crop. There is much hope to develop HCN free mutants in these crops through irradiation in future. Data regarding SDS-PAGE analysis and RAPD technique showed great variability in genetic diversity as a result of gamma irradiation on Sudan grass under study in M-1 and M-2 generations. Further research is needed to improve the crop and develop such mutants by gamma irradiation in future.